TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Dragačević, Luka
AU  - Vujić, Vesna
AU  - Lukić, Ivana
AU  - Stanojević, Stanislava
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/693
AB  - BCG vaccination induces a memory-like response in innate immune cells known as
trained immunity. In this study, we investigated the modification of innate immune
cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with
thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with
a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were
examined for 7 days after TG injection by flow cytometry, and for NO production and
peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage
cells as following: increased in vitro LPS and BCG stimulated NO production from total
cells compared to adherent cells (day 1); increased cell number (days 3 and 5);
increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and
eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on
CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase
activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On
day 7, cells from both experimental groups showed no production of NO in response to
in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the
acute phase of sterile inflammation, which may lead to the later observed phenotypic
and functional changes that could be seen as accelerated resolution of inflammation
and possibly point to trained immune response.
PB  - Wiley
T1  - BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?
EP  - 127
IS  - S2
SP  - 127
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_intor_693
ER  - 

@article{
author = "Ćuruvija, Ivana and Blagojević, Veljko and Dragačević, Luka and Vujić, Vesna and Lukić, Ivana and Stanojević, Stanislava",
year = "2022",
abstract = "BCG vaccination induces a memory-like response in innate immune cells known as
trained immunity. In this study, we investigated the modification of innate immune
cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with
thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with
a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were
examined for 7 days after TG injection by flow cytometry, and for NO production and
peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage
cells as following: increased in vitro LPS and BCG stimulated NO production from total
cells compared to adherent cells (day 1); increased cell number (days 3 and 5);
increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and
eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on
CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase
activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On
day 7, cells from both experimental groups showed no production of NO in response to
in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the
acute phase of sterile inflammation, which may lead to the later observed phenotypic
and functional changes that could be seen as accelerated resolution of inflammation
and possibly point to trained immune response.",
publisher = "Wiley",
title = "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?",
pages = "127-127",
number = "S2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_intor_693"
}

Ćuruvija, I., Blagojević, V., Dragačević, L., Vujić, V., Lukić, I.,& Stanojević, S.. (2022). BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. 
Wiley., 52(S2), 127-127.
https://hdl.handle.net/21.15107/rcub_intor_693

Ćuruvija I, Blagojević V, Dragačević L, Vujić V, Lukić I, Stanojević S. BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. 2022;52(S2):127-127.
https://hdl.handle.net/21.15107/rcub_intor_693 .

Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?", 52, no. S2 (2022):127-127,
https://hdl.handle.net/21.15107/rcub_intor_693 .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Dragačević, Luka
AU  - Vujić, Vesna
AU  - Lukić, Ivana
AU  - Stanojević, Stanislava
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/694
AB  - Inflammation is a redistribution of immune cells, providing a more efficient elimination of the inflammatory offense. However, it is not limited to local microenvironment. In this study, the interaction of the effect of BCG priming and peritoneal inflammation on the remote inflammatory milieu of infected lung was investigated. Young male AO rats infected with Mycoplasma spp. were s.c. injected with BCG (3x105 CFU) or saline, and 7 days later received an i.p. injection of 7ml of thioglycollate (TG) or saline. Up to 7 days after TG injection, a broncho-alveolar lavage (BAL) was performed, and cells were analysed for their surface marker expression and NO production. Infected rats had a high percentage of HIS48HiCD11bHi neutrophils. BCG priming didn’t alter BAL cells phenotype, while TG injection increased the proportion of MHCII+CD11blow activated alveolar macrophages (aAMFs) on day 7. However, the BCG+TG group showed significant changes – percentage of HIS48HiCD11bHi neutrophils decreased from day 3, the share of aAMFs increased from day 5 and the share of MHCII+CD11b-AMFs increased on days 3-5. However, the percentage of B220+FSClow B lymphocytes were increased from day 1. Production of NO from BAL fluid cells was low in all groups. We conclude that BCG vaccination likely increased the number of circulating B lymphocytes, while TG-induced peritoneal inflammation potentially prevented their entry into the peritoneal cavity, forcing them into permissive tissues, such as lungs.
PB  - Wiley
T1  - Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation
EP  - 55
IS  - S2
SP  - 55
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_intor_694
ER  - 

@conference{
author = "Blagojević, Veljko and Ćuruvija, Ivana and Dragačević, Luka and Vujić, Vesna and Lukić, Ivana and Stanojević, Stanislava",
year = "2022",
abstract = "Inflammation is a redistribution of immune cells, providing a more efficient elimination of the inflammatory offense. However, it is not limited to local microenvironment. In this study, the interaction of the effect of BCG priming and peritoneal inflammation on the remote inflammatory milieu of infected lung was investigated. Young male AO rats infected with Mycoplasma spp. were s.c. injected with BCG (3x105 CFU) or saline, and 7 days later received an i.p. injection of 7ml of thioglycollate (TG) or saline. Up to 7 days after TG injection, a broncho-alveolar lavage (BAL) was performed, and cells were analysed for their surface marker expression and NO production. Infected rats had a high percentage of HIS48HiCD11bHi neutrophils. BCG priming didn’t alter BAL cells phenotype, while TG injection increased the proportion of MHCII+CD11blow activated alveolar macrophages (aAMFs) on day 7. However, the BCG+TG group showed significant changes – percentage of HIS48HiCD11bHi neutrophils decreased from day 3, the share of aAMFs increased from day 5 and the share of MHCII+CD11b-AMFs increased on days 3-5. However, the percentage of B220+FSClow B lymphocytes were increased from day 1. Production of NO from BAL fluid cells was low in all groups. We conclude that BCG vaccination likely increased the number of circulating B lymphocytes, while TG-induced peritoneal inflammation potentially prevented their entry into the peritoneal cavity, forcing them into permissive tissues, such as lungs.",
publisher = "Wiley",
title = "Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation",
pages = "55-55",
number = "S2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_intor_694"
}

Blagojević, V., Ćuruvija, I., Dragačević, L., Vujić, V., Lukić, I.,& Stanojević, S.. (2022). Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation. 
Wiley., 52(S2), 55-55.
https://hdl.handle.net/21.15107/rcub_intor_694

Blagojević V, Ćuruvija I, Dragačević L, Vujić V, Lukić I, Stanojević S. Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation. 2022;52(S2):55-55.
https://hdl.handle.net/21.15107/rcub_intor_694 .

Blagojević, Veljko, Ćuruvija, Ivana, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, "Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation", 52, no. S2 (2022):55-55,
https://hdl.handle.net/21.15107/rcub_intor_694 .

TY  - CONF
AU  - Kovačević Jovanović, Vesna
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/673
AB  - BACKGROUND A variety of commensal bacterial taxa elicit serum IgA responses resulting in protection against polymicrobial sepsis, whereas anti-commensal IgG may have deleterious role in gastrointestinal and systemic inflammation. OBJECTIVES The aim was to determine the systemic levels of specific antibodies directed to autologous E.coli in rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains during colitis. METHODS Rats were intrarectally injected with ethanol or trinitrobenzenesulfonic acid (TNBS, 10 or 40mg/kg) whereas controls received saline in the same manner. Sera were tested for the level of anti-E.coli antibodies of IgG1, IgG2a, IgG2b and IgA classes by ELISA. RESULTS Both rat strains developed colitis, but the degree of colon necrosis and hyperemia were slightly greater in DA than in AO rats and the survival rate was significantly lower in DA relative to AO rats. Among saline-treated controls, the levels of IgG1, IgG2a, and IgG2b anti-E.coli antibodies were comparable between rat strains, whereas the amounts of IgA were significantly higher in DA compared to AO rats. Development of colitis significantly increased the amount of anti-E.coli antibodies of IgA and IgG2a classes in sera of AO rats, and those of IgG2a and IgG2b classes in sera of DA rats. Hence, mostly beneficial role of IgA and probably deleterious role of IgG2b antibodies directed to commensal E.coli during colitis may be suggested, whereas the role of anti-E.coli antibodies of IgG2a classes remains intriguing.
PB  - Serbian Society of Microbiology
C3  - FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia
T1  - The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats
SP  - 883
SP  - 883
SP  - 1538
UR  - https://hdl.handle.net/21.15107/rcub_intor_673
ER  - 

@conference{
author = "Kovačević Jovanović, Vesna and Ćuruvija, Ivana and Stanojević, Stanislava and Blagojević, Veljko",
year = "2022",
abstract = "BACKGROUND A variety of commensal bacterial taxa elicit serum IgA responses resulting in protection against polymicrobial sepsis, whereas anti-commensal IgG may have deleterious role in gastrointestinal and systemic inflammation. OBJECTIVES The aim was to determine the systemic levels of specific antibodies directed to autologous E.coli in rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains during colitis. METHODS Rats were intrarectally injected with ethanol or trinitrobenzenesulfonic acid (TNBS, 10 or 40mg/kg) whereas controls received saline in the same manner. Sera were tested for the level of anti-E.coli antibodies of IgG1, IgG2a, IgG2b and IgA classes by ELISA. RESULTS Both rat strains developed colitis, but the degree of colon necrosis and hyperemia were slightly greater in DA than in AO rats and the survival rate was significantly lower in DA relative to AO rats. Among saline-treated controls, the levels of IgG1, IgG2a, and IgG2b anti-E.coli antibodies were comparable between rat strains, whereas the amounts of IgA were significantly higher in DA compared to AO rats. Development of colitis significantly increased the amount of anti-E.coli antibodies of IgA and IgG2a classes in sera of AO rats, and those of IgG2a and IgG2b classes in sera of DA rats. Hence, mostly beneficial role of IgA and probably deleterious role of IgG2b antibodies directed to commensal E.coli during colitis may be suggested, whereas the role of anti-E.coli antibodies of IgG2a classes remains intriguing.",
publisher = "Serbian Society of Microbiology",
journal = "FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia",
title = "The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats",
pages = "883-883-1538",
url = "https://hdl.handle.net/21.15107/rcub_intor_673"
}

Kovačević Jovanović, V., Ćuruvija, I., Stanojević, S.,& Blagojević, V.. (2022). The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats. in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia
Serbian Society of Microbiology., 883.
https://hdl.handle.net/21.15107/rcub_intor_673

Kovačević Jovanović V, Ćuruvija I, Stanojević S, Blagojević V. The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats. in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia. 2022;:883.
https://hdl.handle.net/21.15107/rcub_intor_673 .

Kovačević Jovanović, Vesna, Ćuruvija, Ivana, Stanojević, Stanislava, Blagojević, Veljko, "The intriguing role of anti-commensal bacteria antibodies in sera of colitic rats" in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia (2022):883,
https://hdl.handle.net/21.15107/rcub_intor_673 .

TY  - CONF
AU  - Kovačević Jovanović, Vesna
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/672
AB  - BACKGROUND Deregulation of the immune response to microbiota or pathogens, and increased intestinal permeability have been proposed as disease-driving mechanisms in colitis. Since peritoneal macrophages guard the sterility of peritoneal cavity from bacterial leakage from the gut, it is plausible to assume that peritoneal macrophages are involved in colitis development. OBJECTIVES The objective was to investigate changes in the composition of peritoneal cells and their response to stimulation by selected gut microbiota during colitis. METHODS Seven days following induction of colitis with intrarectal instillation of ethanol or trinitrobenzenesulfonic acid (TNBS, 10mg/kg or 40mg/kg), peritoneal cells of Dark Agouti (DA) rats were isolated and subjected to flow cytometry. The amount of IL-6 and TNF-α produced by adherent cells was determined by ELISA following in vitro stimulation with LPS and commensal E.coli and Enteroccocus spp.
RESULTS
Instillation of ethanol or TNBS (10 and 40 mg/kg) increased the proportion of CD11bintCD4low monocytes
and decreased the proportion of resident CD163+MHCIIIo macrophages and CD163-MHCIIhi macrophage/dendritic cells.
In vitro treatment with Enterococcus spp. was superior over LPS and E.coli in increasing macrophage TNF-α release in all
but saline-injected control rats. In vitro treatment with E.coli exceeded the level of LPS stimulation in
inducing macrophage IL-6 release in saline- and ethanol-injected rats. It may be concluded that changes in the
composition of peritoneal cells during colitis and, subsequently, their selectively altered response to gut commensals
may perpetuate or modulate inflammation during disease development
PB  - Serbian Society of Microbiology
C3  - FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia
T1  - Changes in the composition of rat peritoneal cells and their response to stimulation by selected gut microbiota during colitis
SP  - 880
SP  - 880
SP  - 1536
UR  - https://hdl.handle.net/21.15107/rcub_intor_672
ER  - 

@conference{
author = "Kovačević Jovanović, Vesna and Blagojević, Veljko and Ćuruvija, Ivana and Stanojević, Stanislava",
year = "2022",
abstract = "BACKGROUND Deregulation of the immune response to microbiota or pathogens, and increased intestinal permeability have been proposed as disease-driving mechanisms in colitis. Since peritoneal macrophages guard the sterility of peritoneal cavity from bacterial leakage from the gut, it is plausible to assume that peritoneal macrophages are involved in colitis development. OBJECTIVES The objective was to investigate changes in the composition of peritoneal cells and their response to stimulation by selected gut microbiota during colitis. METHODS Seven days following induction of colitis with intrarectal instillation of ethanol or trinitrobenzenesulfonic acid (TNBS, 10mg/kg or 40mg/kg), peritoneal cells of Dark Agouti (DA) rats were isolated and subjected to flow cytometry. The amount of IL-6 and TNF-α produced by adherent cells was determined by ELISA following in vitro stimulation with LPS and commensal E.coli and Enteroccocus spp.
RESULTS
Instillation of ethanol or TNBS (10 and 40 mg/kg) increased the proportion of CD11bintCD4low monocytes
and decreased the proportion of resident CD163+MHCIIIo macrophages and CD163-MHCIIhi macrophage/dendritic cells.
In vitro treatment with Enterococcus spp. was superior over LPS and E.coli in increasing macrophage TNF-α release in all
but saline-injected control rats. In vitro treatment with E.coli exceeded the level of LPS stimulation in
inducing macrophage IL-6 release in saline- and ethanol-injected rats. It may be concluded that changes in the
composition of peritoneal cells during colitis and, subsequently, their selectively altered response to gut commensals
may perpetuate or modulate inflammation during disease development",
publisher = "Serbian Society of Microbiology",
journal = "FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia",
title = "Changes in the composition of rat peritoneal cells and their response to stimulation by selected gut microbiota during colitis",
pages = "880-880-1536",
url = "https://hdl.handle.net/21.15107/rcub_intor_672"
}

Kovačević Jovanović, V., Blagojević, V., Ćuruvija, I.,& Stanojević, S.. (2022). Changes in the composition of rat peritoneal cells and their response to stimulation by selected gut microbiota during colitis. in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia
Serbian Society of Microbiology., 880.
https://hdl.handle.net/21.15107/rcub_intor_672

Kovačević Jovanović V, Blagojević V, Ćuruvija I, Stanojević S. Changes in the composition of rat peritoneal cells and their response to stimulation by selected gut microbiota during colitis. in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia. 2022;:880.
https://hdl.handle.net/21.15107/rcub_intor_672 .

Kovačević Jovanović, Vesna, Blagojević, Veljko, Ćuruvija, Ivana, Stanojević, Stanislava, "Changes in the composition of rat peritoneal cells and their response to stimulation by selected gut microbiota during colitis" in FEMS conference on microbiology in association with Serbian Society of Microbiology, 30 June - 2 July, Serbia (2022):880,
https://hdl.handle.net/21.15107/rcub_intor_672 .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Veljović, Katarina
AU  - Soković Bajić, Svetlana
AU  - Stanojević, Stanislava
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/667
AB  - We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from 
day 4  to day 30). Feces were collected  for microbial analysis. Colitis was induced at day 85. Seven days later  rats were graded  for histological damage in colon, and samples of 
mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic 
treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well 
as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, 
attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an 
extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. 
The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to 
be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and 
severity of colitis symptoms in untreated rats.
PB  - Wiley
C3  - European Journal of Immunology
T1  - Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats
EP  - 311
SP  - 311
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_intor_667
ER  - 

@conference{
author = "Blagojević, Veljko and Vujić, Vesna and Ćuruvija, Ivana and Veljović, Katarina and Soković Bajić, Svetlana and Stanojević, Stanislava",
year = "2021",
abstract = "We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from 
day 4  to day 30). Feces were collected  for microbial analysis. Colitis was induced at day 85. Seven days later  rats were graded  for histological damage in colon, and samples of 
mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic 
treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well 
as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, 
attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an 
extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. 
The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to 
be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and 
severity of colitis symptoms in untreated rats.",
publisher = "Wiley",
journal = "European Journal of Immunology",
title = "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats",
pages = "311-311",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_intor_667"
}

Blagojević, V., Vujić, V., Ćuruvija, I., Veljović, K., Soković Bajić, S.,& Stanojević, S.. (2021). Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology
Wiley., 51, 311-311.
https://hdl.handle.net/21.15107/rcub_intor_667

Blagojević V, Vujić V, Ćuruvija I, Veljović K, Soković Bajić S, Stanojević S. Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology. 2021;51:311-311.
https://hdl.handle.net/21.15107/rcub_intor_667 .

Blagojević, Veljko, Vujić, Vesna, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Stanojević, Stanislava, "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats" in European Journal of Immunology, 51 (2021):311-311,
https://hdl.handle.net/21.15107/rcub_intor_667 .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujić, Vesna
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/611
AB  - Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.
PB  - Springer Nature
T2  - Inflammation
T1  - Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity
DO  - 10.1007/s10753-021-01513-z
ER  - 

@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujić, Vesna",
year = "2021",
abstract = "Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.",
publisher = "Springer Nature",
journal = "Inflammation",
title = "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity",
doi = "10.1007/s10753-021-01513-z"
}

Stanojević, S., Blagojević, V., Ćuruvija, I.,& Vujić, V.. (2021). Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation
Springer Nature..
https://doi.org/10.1007/s10753-021-01513-z

Stanojević S, Blagojević V, Ćuruvija I, Vujić V. Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation. 2021;.
doi:10.1007/s10753-021-01513-z .

Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujić, Vesna, "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity" in Inflammation (2021),
https://doi.org/10.1007/s10753-021-01513-z . .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Ćuruvija, Ivana
AU  - Prijić, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/665
AB  - Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"
PB  - University of Belgrade; Immunological Society of Serbia
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia
T1  - Potential impact of early-life probiotic supplementation on peritoneal macrophage function
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_intor_665
ER  - 

@conference{
author = "Blagojević, Veljko and Petrović, Raisa and Ćuruvija, Ivana and Prijić, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2019",
abstract = "Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia",
title = "Potential impact of early-life probiotic supplementation on peritoneal macrophage function",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_intor_665"
}

Blagojević, V., Petrović, R., Ćuruvija, I., Prijić, I., Vujić, V.,& Stanojević, S.. (2019). Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"., 34.
https://hdl.handle.net/21.15107/rcub_intor_665

Blagojević V, Petrović R, Ćuruvija I, Prijić I, Vujić V, Stanojević S. Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. 2019;:34.
https://hdl.handle.net/21.15107/rcub_intor_665 .

Blagojević, Veljko, Petrović, Raisa, Ćuruvija, Ivana, Prijić, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Potential impact of early-life probiotic supplementation on peritoneal macrophage function" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia (2019):34,
https://hdl.handle.net/21.15107/rcub_intor_665 .

TY  - CONF
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Prijić, Ivana
AU  - Vujić, Vesna
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/664
AB  - Aging differently affects the expression of estrogen receptors alpha and beta
(ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of
male and female rats. We explored the involvement of ERα and ERβ in the in
vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol
(which stimulates both receptors) or genistein (which is predominantly an ERβ
agonist) on inflammatory cytokine secretion from young (3 months old) and
middle-aged (16 months old) female and male AO rats. Aging diminished the
proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages
from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via
ERα, and suppressed it in cells from young females via ERβ. Genistein-induced
decrease of IL-1β in macrophages from all experimental groups was probably
mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all
experimental groups via ERα while genistein diminished it in all females’ and
in middle-aged male rats’ macrophages by activating ERβ. However, genistein
increased IL-6 secretion from macrophages of young male rats via ERα.
Although 17β-estradiol and genistein stimulated secretion of macrophage
inflammatory cytokines via ERα and suppressed it probably via ERβ, their
modulatory actions were determined by aging-induced changes in macrophage
ERs expression and possible ERα / ERβ interactions (Supported by Ministry of
Education, Science and Technological development, Republic of Serbia, Grant
No 175050).
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"
PB  - University of Belgrade; Immunological Society of Serbia
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.
T1  - 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner
EP  - 132
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_intor_664
ER  - 

@conference{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna",
year = "2019",
abstract = "Aging differently affects the expression of estrogen receptors alpha and beta
(ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of
male and female rats. We explored the involvement of ERα and ERβ in the in
vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol
(which stimulates both receptors) or genistein (which is predominantly an ERβ
agonist) on inflammatory cytokine secretion from young (3 months old) and
middle-aged (16 months old) female and male AO rats. Aging diminished the
proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages
from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via
ERα, and suppressed it in cells from young females via ERβ. Genistein-induced
decrease of IL-1β in macrophages from all experimental groups was probably
mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all
experimental groups via ERα while genistein diminished it in all females’ and
in middle-aged male rats’ macrophages by activating ERβ. However, genistein
increased IL-6 secretion from macrophages of young male rats via ERα.
Although 17β-estradiol and genistein stimulated secretion of macrophage
inflammatory cytokines via ERα and suppressed it probably via ERβ, their
modulatory actions were determined by aging-induced changes in macrophage
ERs expression and possible ERα / ERβ interactions (Supported by Ministry of
Education, Science and Technological development, Republic of Serbia, Grant
No 175050).",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.",
title = "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner",
pages = "132-132",
url = "https://hdl.handle.net/21.15107/rcub_intor_664"
}

Ćuruvija, I., Stanojević, S., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2019). 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"., 132-132.
https://hdl.handle.net/21.15107/rcub_intor_664

Ćuruvija I, Stanojević S, Blagojević V, Petrović R, Prijić I, Vujić V. 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.. 2019;:132-132.
https://hdl.handle.net/21.15107/rcub_intor_664 .

Ćuruvija, Ivana, Stanojević, Stanislava, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. (2019):132-132,
https://hdl.handle.net/21.15107/rcub_intor_664 .

TY  - JOUR
AU  - Blagojević, Veljko
AU  - Kovačević-Jovanović, Vesna
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/512
AB  - Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
EP  - 157
SP  - 147
VL  - 197
DO  - 10.1016/j.lfs.2018.02.011
ER  - 

@article{
author = "Blagojević, Veljko and Kovačević-Jovanović, Vesna and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2018",
abstract = "Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?",
pages = "157-147",
volume = "197",
doi = "10.1016/j.lfs.2018.02.011"
}

Blagojević, V., Kovačević-Jovanović, V., Ćuruvija, I., Petrović, R., Vujnović, I., Vujić, V.,& Stanojević, S.. (2018). Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 197, 147-157.
https://doi.org/10.1016/j.lfs.2018.02.011

Blagojević V, Kovačević-Jovanović V, Ćuruvija I, Petrović R, Vujnović I, Vujić V, Stanojević S. Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences. 2018;197:147-157.
doi:10.1016/j.lfs.2018.02.011 .

Blagojević, Veljko, Kovačević-Jovanović, Vesna, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?" in Life Sciences, 197 (2018):147-157,
https://doi.org/10.1016/j.lfs.2018.02.011 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Prijić, Ivana
AU  - Vujić, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/511
AB  - The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats
EP  - 94
SP  - 86
VL  - 113
DO  - 10.1016/j.exger.2018.09.024
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna",
year = "2018",
abstract = "The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats",
pages = "94-86",
volume = "113",
doi = "10.1016/j.exger.2018.09.024"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2018). The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 113, 86-94.
https://doi.org/10.1016/j.exger.2018.09.024

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Prijić I, Vujić V. The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology. 2018;113:86-94.
doi:10.1016/j.exger.2018.09.024 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats" in Experimental Gerontology, 113 (2018):86-94,
https://doi.org/10.1016/j.exger.2018.09.024 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Veljović, Katarina
AU  - Soković-Bajić, Svetlana
AU  - Kotur-Stevuljević, Jelena
AU  - Bogdanović, Andrija
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Kovačević-Jovanović, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/505
AB  - The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis
EP  - 105
SP  - 92
VL  - 48
DO  - 10.1016/j.jff.2018.07.014
ER  - 

@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Veljović, Katarina and Soković-Bajić, Svetlana and Kotur-Stevuljević, Jelena and Bogdanović, Andrija and Petrović, Raisa and Vujnović, Ivana and Kovačević-Jovanović, Vesna",
year = "2018",
abstract = "The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis",
pages = "105-92",
volume = "48",
doi = "10.1016/j.jff.2018.07.014"
}

Stanojević, S., Blagojević, V., Ćuruvija, I., Veljović, K., Soković-Bajić, S., Kotur-Stevuljević, J., Bogdanović, A., Petrović, R., Vujnović, I.,& Kovačević-Jovanović, V.. (2018). Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 48, 92-105.
https://doi.org/10.1016/j.jff.2018.07.014

Stanojević S, Blagojević V, Ćuruvija I, Veljović K, Soković-Bajić S, Kotur-Stevuljević J, Bogdanović A, Petrović R, Vujnović I, Kovačević-Jovanović V. Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods. 2018;48:92-105.
doi:10.1016/j.jff.2018.07.014 .

Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Veljović, Katarina, Soković-Bajić, Svetlana, Kotur-Stevuljević, Jelena, Bogdanović, Andrija, Petrović, Raisa, Vujnović, Ivana, Kovačević-Jovanović, Vesna, "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis" in Journal of Functional Foods, 48 (2018):92-105,
https://doi.org/10.1016/j.jff.2018.07.014 . .

TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Arsenović-Ranin, Nevena
AU  - Blagojević, Veljko
AU  - Dimitrijević, Mirjana
AU  - Vidić-Danković, Biljana
AU  - Vujić, Vesna
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/496
AB  - The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression
EP  - 1101
IS  - 3
SP  - 1087
VL  - 40
DO  - 10.1007/s10753-017-0551-3
ER  - 

@article{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Arsenović-Ranin, Nevena and Blagojević, Veljko and Dimitrijević, Mirjana and Vidić-Danković, Biljana and Vujić, Vesna",
year = "2017",
abstract = "The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression",
pages = "1101-1087",
number = "3",
volume = "40",
doi = "10.1007/s10753-017-0551-3"
}

Ćuruvija, I., Stanojević, S., Arsenović-Ranin, N., Blagojević, V., Dimitrijević, M., Vidić-Danković, B.,& Vujić, V.. (2017). Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation
Springer/Plenum Publishers, New York., 40(3), 1087-1101.
https://doi.org/10.1007/s10753-017-0551-3

Ćuruvija I, Stanojević S, Arsenović-Ranin N, Blagojević V, Dimitrijević M, Vidić-Danković B, Vujić V. Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation. 2017;40(3):1087-1101.
doi:10.1007/s10753-017-0551-3 .

Ćuruvija, Ivana, Stanojević, Stanislava, Arsenović-Ranin, Nevena, Blagojević, Veljko, Dimitrijević, Mirjana, Vidić-Danković, Biljana, Vujić, Vesna, "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression" in Inflammation, 40, no. 3 (2017):1087-1101,
https://doi.org/10.1007/s10753-017-0551-3 . .

TY  - CONF
AU  - Petrović, Raisa
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujnović, Ivana
AU  - Arsenović-Ranin, Nevena
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/669
AB  - Cilj rada je bio da se ispita uticaj starenja na aktivnost makrofaga aktivisanih M1/M2 polarizujućim faktorima. Peritonealne rezidentne makrofage (rMf) i tioglikolatom-indukovane makrofage (trFm) mladih (3 meseca) i starih (18-19) meseci pacova su kultivisane u prisustvu stimulatora klasične (M1) – lipopolisaharida (LPS) i faktora stimulacije kolonija granulocita i makrofaga (GM-CSF), i alternativne (M2) aktivacije makrofaga – interleukina-4 (IL-4), ili u odsustvu poznatih simulatora. Ispitivana je sposobnost fagocitozr zimozana i sekrecije inflamatornih medijatora. Starenjem se povećavala učestalost makrofaga monocitnog porekla (CCR*7CD68* ćelije) u okviru populacije rMf, dok je u okviru populacije tgMf nađena povećana učestalost makrofaga najzrelijeg fenotipa (CD163*CD68* ćelije). Nijedan od ispitivanih stimulatora nije uticao na fagocitnu sposobnost rMf i tgMf. Povećana sekrecija IL-1β, IL-6 I IL-10 I smanjena sekrecija TGF-β u odgovoru na stimulaciju LPS-om je nađena kod rMf i tgMF pacova obe starosti. GM-CSF je povećao sekreciju  IL-1β i IL-6 kod rMf starih pacova i tgMf mladih pacova. Paradoksalno, IL-4 je povećao sekreciju pro-inflamatornih citokina,  IL-1β i IL-6, kod rMf starih pacova. Starenjem se metabolizam arginina i u rMf i u tgMf usmeravao ka sintezi uree. Rezultati su pokazali da sa starenjem tgMf gube sposobnost polatizacije pod uticajem GM-CSF, i da rMf pod uticajem IL-4 polarizuju prema pro-inflamatornom M1 sekretornom fenotipu. Gubitak kontrole sekrecije inflamatornih medijatora iz makrofaga u odgovoru na M1/M2 aktivatore mogao bi da doprinese povećanom riziku od oboljevanja od infektivnih i inflamatornih bolesti u starosti.
C3  - VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata
T1  - Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro
UR  - https://hdl.handle.net/21.15107/rcub_intor_669
ER  - 

@conference{
author = "Petrović, Raisa and Dimitrijević, Mirjana and Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujnović, Ivana and Arsenović-Ranin, Nevena and Vujić, Vesna and Leposavić, Gordana",
year = "2016",
abstract = "Cilj rada je bio da se ispita uticaj starenja na aktivnost makrofaga aktivisanih M1/M2 polarizujućim faktorima. Peritonealne rezidentne makrofage (rMf) i tioglikolatom-indukovane makrofage (trFm) mladih (3 meseca) i starih (18-19) meseci pacova su kultivisane u prisustvu stimulatora klasične (M1) – lipopolisaharida (LPS) i faktora stimulacije kolonija granulocita i makrofaga (GM-CSF), i alternativne (M2) aktivacije makrofaga – interleukina-4 (IL-4), ili u odsustvu poznatih simulatora. Ispitivana je sposobnost fagocitozr zimozana i sekrecije inflamatornih medijatora. Starenjem se povećavala učestalost makrofaga monocitnog porekla (CCR*7CD68* ćelije) u okviru populacije rMf, dok je u okviru populacije tgMf nađena povećana učestalost makrofaga najzrelijeg fenotipa (CD163*CD68* ćelije). Nijedan od ispitivanih stimulatora nije uticao na fagocitnu sposobnost rMf i tgMf. Povećana sekrecija IL-1β, IL-6 I IL-10 I smanjena sekrecija TGF-β u odgovoru na stimulaciju LPS-om je nađena kod rMf i tgMF pacova obe starosti. GM-CSF je povećao sekreciju  IL-1β i IL-6 kod rMf starih pacova i tgMf mladih pacova. Paradoksalno, IL-4 je povećao sekreciju pro-inflamatornih citokina,  IL-1β i IL-6, kod rMf starih pacova. Starenjem se metabolizam arginina i u rMf i u tgMf usmeravao ka sintezi uree. Rezultati su pokazali da sa starenjem tgMf gube sposobnost polatizacije pod uticajem GM-CSF, i da rMf pod uticajem IL-4 polarizuju prema pro-inflamatornom M1 sekretornom fenotipu. Gubitak kontrole sekrecije inflamatornih medijatora iz makrofaga u odgovoru na M1/M2 aktivatore mogao bi da doprinese povećanom riziku od oboljevanja od infektivnih i inflamatornih bolesti u starosti.",
journal = "VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata",
title = "Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro",
url = "https://hdl.handle.net/21.15107/rcub_intor_669"
}

Petrović, R., Dimitrijević, M., Stanojević, S., Blagojević, V., Ćuruvija, I., Vujnović, I., Arsenović-Ranin, N., Vujić, V.,& Leposavić, G.. (2016). Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata.
https://hdl.handle.net/21.15107/rcub_intor_669

Petrović R, Dimitrijević M, Stanojević S, Blagojević V, Ćuruvija I, Vujnović I, Arsenović-Ranin N, Vujić V, Leposavić G. Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata. 2016;.
https://hdl.handle.net/21.15107/rcub_intor_669 .

Petrović, Raisa, Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Arsenović-Ranin, Nevena, Vujić, Vesna, Leposavić, Gordana, "Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro" in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata (2016),
https://hdl.handle.net/21.15107/rcub_intor_669 .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujnović, Ivana
AU  - Petrović, Raisa
AU  - Arsenović-Ranin, Nevena
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/466
AB  - Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4
EP  - 371
IS  - 2
SP  - 359
VL  - 17
DO  - 10.1007/s10522-015-9620-x
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujnović, Ivana and Petrović, Raisa and Arsenović-Ranin, Nevena and Vujić, Vesna and Leposavić, Gordana",
year = "2016",
abstract = "Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4",
pages = "371-359",
number = "2",
volume = "17",
doi = "10.1007/s10522-015-9620-x"
}

Dimitrijević, M., Stanojević, S., Blagojević, V., Ćuruvija, I., Vujnović, I., Petrović, R., Arsenović-Ranin, N., Vujić, V.,& Leposavić, G.. (2016). Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology
Springer, New York., 17(2), 359-371.
https://doi.org/10.1007/s10522-015-9620-x

Dimitrijević M, Stanojević S, Blagojević V, Ćuruvija I, Vujnović I, Petrović R, Arsenović-Ranin N, Vujić V, Leposavić G. Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology. 2016;17(2):359-371.
doi:10.1007/s10522-015-9620-x .

Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Petrović, Raisa, Arsenović-Ranin, Nevena, Vujić, Vesna, Leposavić, Gordana, "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4" in Biogerontology, 17, no. 2 (2016):359-371,
https://doi.org/10.1007/s10522-015-9620-x . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/453
AB  - Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging
EP  - 107
SP  - 95
VL  - 85
DO  - 10.1016/j.exger.2016.10.005
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2016",
abstract = "Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging",
pages = "107-95",
volume = "85",
doi = "10.1016/j.exger.2016.10.005"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Vujić, V.,& Dimitrijević, M.. (2016). Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 85, 95-107.
https://doi.org/10.1016/j.exger.2016.10.005

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Vujić V, Dimitrijević M. Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology. 2016;85:95-107.
doi:10.1016/j.exger.2016.10.005 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Vujić, Vesna, Dimitrijević, Mirjana, "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging" in Experimental Gerontology, 85 (2016):95-107,
https://doi.org/10.1016/j.exger.2016.10.005 . .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, I.
AU  - Dimitrijević, Mirjana
AU  - Vujić, V.
AU  - Stanojević, Stanislava
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/671
AB  - The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.
C3  - 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book
T1  - Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4
EP  - 341
SP  - 341
SP  - P.C.12.06
UR  - https://hdl.handle.net/21.15107/rcub_intor_671
ER  - 

@conference{
author = "Blagojević, Veljko and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, I. and Dimitrijević, Mirjana and Vujić, V. and Stanojević, Stanislava and Leposavić, Gordana",
year = "2015",
abstract = "The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.",
journal = "4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book",
title = "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4",
pages = "341-341-P.C.12.06",
url = "https://hdl.handle.net/21.15107/rcub_intor_671"
}

Blagojević, V., Ćuruvija, I., Petrović, R., Vujnović, I., Dimitrijević, M., Vujić, V., Stanojević, S.,& Leposavić, G.. (2015). Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book, 341-341.
https://hdl.handle.net/21.15107/rcub_intor_671

Blagojević V, Ćuruvija I, Petrović R, Vujnović I, Dimitrijević M, Vujić V, Stanojević S, Leposavić G. Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book. 2015;:341-341.
https://hdl.handle.net/21.15107/rcub_intor_671 .

Blagojević, Veljko, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, I., Dimitrijević, Mirjana, Vujić, V., Stanojević, Stanislava, Leposavić, Gordana, "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4" in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book (2015):341-341,
https://hdl.handle.net/21.15107/rcub_intor_671 .

TY  - CONF
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Vujnović, Ivana
AU  - Petrović, Raisa
AU  - Dimitrijević, Mirjana
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/666
AB  - The present study was designed to examine influence of aging on
macrophage proinflammatory/anti-inflammatory capacity in rat model of
thioglycollate-induced peritonitis. Peritoneal macrophages were isolated
from young (3-months-old) and aged (18-months-old) Dark Agouti (DA)
and Albino Oxford (AO) rats seven days post-injection of thioglycollate
medium. Freshly isolated peritoneal exudate cells were examined for the
expression of CD163, CCR7, CD14 and TLR4, whereas cytokine
production (TNF-α, IL-6 and IL-10) and arginine metabolism end-products
(NO and urea) were assayed in vitro under basal conditions and following
stimulation with LPS. In DA rat inflammatory peritoneal exudate, aging
diminished the frequency of cells with a “resolving macrophage”
CD14+CD163+ phenotype. However, in AO rats, which exhibited stable
frequency of CD14+CD163+ cells in inflammatory peritoneal exudate with
aging, the proportion of CCR7-bearing peritoneal cells, presumably
immigrating inflammatory monocytes, was diminished in aged animals.
Under basal culture conditions, macrophages from aged rats of both strains
released less amount of TNF-α, IL-6 and IL-10, but produced more urea
than cells from young strain-matched rats. However, these changes were
more pronounced in peritoneal macrophages from AO rats. Additionally,
age-related decrease in the frequency of TLR4-expressing cells was
observed among fresh peritoneal exudate cells from AO rats. Upon LPS
stimulation, the production of prototypic inflammatory cytokines (TNF-α
and IL-6) was diminished in macrophages from aged AO rats, whereas
aging had the opposite effect on their production in DA rat macrophages.
Moreover, aging increased NO production in LPS-stimulated macrophages
from DA rats, whereas urea production was enhanced in macrophages from
both strains, but this increase was strikingly more pronounced in
macrophages from AO rats. Collectively, results suggest that aging affects
inflammatory peritoneal exudate cellular composition and macrophage
proinflamatory/immunomodulatory capacity in a strain- specific manner
PB  - Immunological society of Serbia
PB  - University of Kragujevac, Faculty of medical sciences
C3  - 3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015
T1  - Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner
EP  - 58
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_intor_666
ER  - 

@conference{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Vujnović, Ivana and Petrović, Raisa and Dimitrijević, Mirjana and Leposavić, Gordana",
year = "2015",
abstract = "The present study was designed to examine influence of aging on
macrophage proinflammatory/anti-inflammatory capacity in rat model of
thioglycollate-induced peritonitis. Peritoneal macrophages were isolated
from young (3-months-old) and aged (18-months-old) Dark Agouti (DA)
and Albino Oxford (AO) rats seven days post-injection of thioglycollate
medium. Freshly isolated peritoneal exudate cells were examined for the
expression of CD163, CCR7, CD14 and TLR4, whereas cytokine
production (TNF-α, IL-6 and IL-10) and arginine metabolism end-products
(NO and urea) were assayed in vitro under basal conditions and following
stimulation with LPS. In DA rat inflammatory peritoneal exudate, aging
diminished the frequency of cells with a “resolving macrophage”
CD14+CD163+ phenotype. However, in AO rats, which exhibited stable
frequency of CD14+CD163+ cells in inflammatory peritoneal exudate with
aging, the proportion of CCR7-bearing peritoneal cells, presumably
immigrating inflammatory monocytes, was diminished in aged animals.
Under basal culture conditions, macrophages from aged rats of both strains
released less amount of TNF-α, IL-6 and IL-10, but produced more urea
than cells from young strain-matched rats. However, these changes were
more pronounced in peritoneal macrophages from AO rats. Additionally,
age-related decrease in the frequency of TLR4-expressing cells was
observed among fresh peritoneal exudate cells from AO rats. Upon LPS
stimulation, the production of prototypic inflammatory cytokines (TNF-α
and IL-6) was diminished in macrophages from aged AO rats, whereas
aging had the opposite effect on their production in DA rat macrophages.
Moreover, aging increased NO production in LPS-stimulated macrophages
from DA rats, whereas urea production was enhanced in macrophages from
both strains, but this increase was strikingly more pronounced in
macrophages from AO rats. Collectively, results suggest that aging affects
inflammatory peritoneal exudate cellular composition and macrophage
proinflamatory/immunomodulatory capacity in a strain- specific manner",
publisher = "Immunological society of Serbia, University of Kragujevac, Faculty of medical sciences",
journal = "3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015",
title = "Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_intor_666"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Vujnović, I., Petrović, R., Dimitrijević, M.,& Leposavić, G.. (2015). Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner. in 3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015
Immunological society of Serbia., 58-58.
https://hdl.handle.net/21.15107/rcub_intor_666

Stanojević S, Ćuruvija I, Blagojević V, Vujnović I, Petrović R, Dimitrijević M, Leposavić G. Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner. in 3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015. 2015;:58-58.
https://hdl.handle.net/21.15107/rcub_intor_666 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Vujnović, Ivana, Petrović, Raisa, Dimitrijević, Mirjana, Leposavić, Gordana, "Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner" in 3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015 (2015):58-58,
https://hdl.handle.net/21.15107/rcub_intor_666 .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/423
AB  - Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
EP  - 456
IS  - 5
SP  - 445
VL  - 74
DO  - 10.1111/aji.12424
ER  - 

@article{
author = "Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana and Vujić, Vesna and Ćuruvija, Ivana and Blagojević, Veljko and Leposavić, Gordana",
year = "2015",
abstract = "Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat",
pages = "456-445",
number = "5",
volume = "74",
doi = "10.1111/aji.12424"
}

Stanojević, S., Kovačević-Jovanović, V., Dimitrijević, M., Vujić, V., Ćuruvija, I., Blagojević, V.,& Leposavić, G.. (2015). Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology
Wiley, Hoboken., 74(5), 445-456.
https://doi.org/10.1111/aji.12424

Stanojević S, Kovačević-Jovanović V, Dimitrijević M, Vujić V, Ćuruvija I, Blagojević V, Leposavić G. Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology. 2015;74(5):445-456.
doi:10.1111/aji.12424 .

Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Ćuruvija, Ivana, Blagojević, Veljko, Leposavić, Gordana, "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat" in American Journal of Reproductive Immunology, 74, no. 5 (2015):445-456,
https://doi.org/10.1111/aji.12424 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/449
AB  - We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats
EP  - 19
IS  - 1
SP  - 1
VL  - 62
DO  - 10.1556/AMicr.62.2015.1.1
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2015",
abstract = "We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats",
pages = "19-1",
number = "1",
volume = "62",
doi = "10.1556/AMicr.62.2015.1.1"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2015). Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Zrt, Budapest., 62(1), 1-19.
https://doi.org/10.1556/AMicr.62.2015.1.1

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica. 2015;62(1):1-19.
doi:10.1556/AMicr.62.2015.1.1 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats" in Acta Microbiologica et Immunologica Hungarica, 62, no. 1 (2015):1-19,
https://doi.org/10.1556/AMicr.62.2015.1.1 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/424
AB  - This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains
EP  - 1449
IS  - 4
SP  - 1434
VL  - 38
DO  - 10.1007/s10753-015-0118-0
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2015",
abstract = "This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains",
pages = "1449-1434",
number = "4",
volume = "38",
doi = "10.1007/s10753-015-0118-0"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V.,& Dimitrijević, M.. (2015). Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation
Springer/Plenum Publishers, New York., 38(4), 1434-1449.
https://doi.org/10.1007/s10753-015-0118-0

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Dimitrijević M. Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation. 2015;38(4):1434-1449.
doi:10.1007/s10753-015-0118-0 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains" in Inflammation, 38, no. 4 (2015):1434-1449,
https://doi.org/10.1007/s10753-015-0118-0 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/402
AB  - Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains
EP  - 486
IS  - 5
SP  - 475
VL  - 15
DO  - 10.1007/s10522-014-9513-4
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Vujić, Vesna and Aleksić, Iva and Pilipović, Ivan and Leposavić, Gordana",
year = "2014",
abstract = "Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains",
pages = "486-475",
number = "5",
volume = "15",
doi = "10.1007/s10522-014-9513-4"
}

Dimitrijević, M., Stanojević, S., Vujić, V., Aleksić, I., Pilipović, I.,& Leposavić, G.. (2014). Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology
Springer, New York., 15(5), 475-486.
https://doi.org/10.1007/s10522-014-9513-4

Dimitrijević M, Stanojević S, Vujić V, Aleksić I, Pilipović I, Leposavić G. Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology. 2014;15(5):475-486.
doi:10.1007/s10522-014-9513-4 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Vujić, Vesna, Aleksić, Iva, Pilipović, Ivan, Leposavić, Gordana, "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains" in Biogerontology, 15, no. 5 (2014):475-486,
https://doi.org/10.1007/s10522-014-9513-4 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Aleksić, Iva
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Pilipović, Ivan
AU  - von Hoersten, Stephan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/397
AB  - In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
PB  - Springer, Dordrecht
T2  - AGE
T1  - Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro
IS  - 4
VL  - 36
DO  - 10.1007/s11357-014-9696-2
ER  - 

@article{
author = "Dimitrijević, Mirjana and Aleksić, Iva and Vujić, Vesna and Stanojević, Stanislava and Pilipović, Ivan and von Hoersten, Stephan and Leposavić, Gordana",
year = "2014",
abstract = "In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.",
publisher = "Springer, Dordrecht",
journal = "AGE",
title = "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro",
number = "4",
volume = "36",
doi = "10.1007/s11357-014-9696-2"
}

Dimitrijević, M., Aleksić, I., Vujić, V., Stanojević, S., Pilipović, I., von Hoersten, S.,& Leposavić, G.. (2014). Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE
Springer, Dordrecht., 36(4).
https://doi.org/10.1007/s11357-014-9696-2

Dimitrijević M, Aleksić I, Vujić V, Stanojević S, Pilipović I, von Hoersten S, Leposavić G. Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE. 2014;36(4).
doi:10.1007/s11357-014-9696-2 .

Dimitrijević, Mirjana, Aleksić, Iva, Vujić, Vesna, Stanojević, Stanislava, Pilipović, Ivan, von Hoersten, Stephan, Leposavić, Gordana, "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro" in AGE, 36, no. 4 (2014),
https://doi.org/10.1007/s11357-014-9696-2 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/391
AB  - For many years, the central nervous system and the immune system were considered two autonomous entities. However, extensive research in the field of neuroimmunomodulation during the past decades has demonstrated the presence of different neuropeptides and their respective receptors in the immune cells. More importantly, it has provided evidence for the direct effects of neuropeptides on the immune cell functions. Neuropeptide Y (NPY) is generally considered the most abundant peptide in the central and peripheral nervous system. However, it is also distinguished by exhibiting pleiotropic functions in many other physiological systems, including the immune system. NPY affects the functions of the cells of the adaptive and innate immunity. In this respect, NPY is known to modulate immune cell trafficking, T helper cell differentiation, cytokine secretion, natural killer cell activity, phagocytosis and the production of reactive oxygen species. The specific Y receptors have been found in immune cells, and their expression is amplified upon immune stimulation. Different Y receptor subtypes may mediate an opposite effect of NPY on the particular function, thus underlining its regulatory role. Since the immune cells are capable of producing NPY upon appropriate stimulation, this peptide can regulate immune cell functions in an autocrine/paracrine manner. NPY also has important implications in several immune-mediated disorders, which affirms the clear need for further investigation of its role in either the mechanisms of the disease development or its possible therapeutic capacity. This review summarises the key points of NPY's mission throughout the immune system.
PB  - Springer Wien, Wien
T2  - Amino Acids
T1  - The intriguing mission of neuropeptide Y in the immune system
EP  - 53
IS  - 1
SP  - 41
VL  - 45
DO  - 10.1007/s00726-011-1185-7
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2013",
abstract = "For many years, the central nervous system and the immune system were considered two autonomous entities. However, extensive research in the field of neuroimmunomodulation during the past decades has demonstrated the presence of different neuropeptides and their respective receptors in the immune cells. More importantly, it has provided evidence for the direct effects of neuropeptides on the immune cell functions. Neuropeptide Y (NPY) is generally considered the most abundant peptide in the central and peripheral nervous system. However, it is also distinguished by exhibiting pleiotropic functions in many other physiological systems, including the immune system. NPY affects the functions of the cells of the adaptive and innate immunity. In this respect, NPY is known to modulate immune cell trafficking, T helper cell differentiation, cytokine secretion, natural killer cell activity, phagocytosis and the production of reactive oxygen species. The specific Y receptors have been found in immune cells, and their expression is amplified upon immune stimulation. Different Y receptor subtypes may mediate an opposite effect of NPY on the particular function, thus underlining its regulatory role. Since the immune cells are capable of producing NPY upon appropriate stimulation, this peptide can regulate immune cell functions in an autocrine/paracrine manner. NPY also has important implications in several immune-mediated disorders, which affirms the clear need for further investigation of its role in either the mechanisms of the disease development or its possible therapeutic capacity. This review summarises the key points of NPY's mission throughout the immune system.",
publisher = "Springer Wien, Wien",
journal = "Amino Acids",
title = "The intriguing mission of neuropeptide Y in the immune system",
pages = "53-41",
number = "1",
volume = "45",
doi = "10.1007/s00726-011-1185-7"
}

Dimitrijević, M.,& Stanojević, S.. (2013). The intriguing mission of neuropeptide Y in the immune system. in Amino Acids
Springer Wien, Wien., 45(1), 41-53.
https://doi.org/10.1007/s00726-011-1185-7

Dimitrijević M, Stanojević S. The intriguing mission of neuropeptide Y in the immune system. in Amino Acids. 2013;45(1):41-53.
doi:10.1007/s00726-011-1185-7 .

Dimitrijević, Mirjana, Stanojević, Stanislava, "The intriguing mission of neuropeptide Y in the immune system" in Amino Acids, 45, no. 1 (2013):41-53,
https://doi.org/10.1007/s00726-011-1185-7 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/389
AB  - New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.
PB  - Wiley-Blackwell, Hoboken
T2  - Experimental Physiology
T1  - Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production
EP  - 678
IS  - 3
SP  - 665
VL  - 98
DO  - 10.1113/expphysiol.2012.070524
ER  - 

@article{
author = "Stanojević, Stanislava and Dimitrijević, Mirjana and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Leposavić, Gordana",
year = "2013",
abstract = "New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Experimental Physiology",
title = "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production",
pages = "678-665",
number = "3",
volume = "98",
doi = "10.1113/expphysiol.2012.070524"
}

Stanojević, S., Dimitrijević, M., Kuštrimović, N., Mitić, K., Vujić, V.,& Leposavić, G.. (2013). Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology
Wiley-Blackwell, Hoboken., 98(3), 665-678.
https://doi.org/10.1113/expphysiol.2012.070524

Stanojević S, Dimitrijević M, Kuštrimović N, Mitić K, Vujić V, Leposavić G. Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology. 2013;98(3):665-678.
doi:10.1113/expphysiol.2012.070524 .

Stanojević, Stanislava, Dimitrijević, Mirjana, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Leposavić, Gordana, "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production" in Experimental Physiology, 98, no. 3 (2013):665-678,
https://doi.org/10.1113/expphysiol.2012.070524 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/386
AB  - We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Strain differences in the humoral immune response to commensal bacterial antigens in rats
EP  - 288
IS  - 3
SP  - 271
VL  - 60
DO  - 10.1556/AMicr.60.2013.3.4
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2013",
abstract = "We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Strain differences in the humoral immune response to commensal bacterial antigens in rats",
pages = "288-271",
number = "3",
volume = "60",
doi = "10.1556/AMicr.60.2013.3.4"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2013). Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 60(3), 271-288.
https://doi.org/10.1556/AMicr.60.2013.3.4

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica. 2013;60(3):271-288.
doi:10.1556/AMicr.60.2013.3.4 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences in the humoral immune response to commensal bacterial antigens in rats" in Acta Microbiologica et Immunologica Hungarica, 60, no. 3 (2013):271-288,
https://doi.org/10.1556/AMicr.60.2013.3.4 . .