BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?
Само за регистроване кориснике
2022
Аутори
Ćuruvija, IvanaBlagojević, Veljko
Dragačević, Luka
Vujić, Vesna
Lukić, Ivana
Stanojević, Stanislava
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BCG vaccination induces a memory-like response in innate immune cells known as
trained immunity. In this study, we investigated the modification of innate immune
cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with
thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with
a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were
examined for 7 days after TG injection by flow cytometry, and for NO production and
peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage
cells as following: increased in vitro LPS and BCG stimulated NO production from total
cells compared to adherent cells (day 1); increased cell number (days 3 and 5);
increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and
eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on
CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated pero...xidase
activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On
day 7, cells from both experimental groups showed no production of NO in response to
in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the
acute phase of sterile inflammation, which may lead to the later observed phenotypic
and functional changes that could be seen as accelerated resolution of inflammation
and possibly point to trained immune response.
Извор:
2022, 52, S2, 127-127Издавач:
- Wiley
Институција/група
TorlakTY - JOUR AU - Ćuruvija, Ivana AU - Blagojević, Veljko AU - Dragačević, Luka AU - Vujić, Vesna AU - Lukić, Ivana AU - Stanojević, Stanislava PY - 2022 UR - http://intor.torlakinstitut.com/handle/123456789/693 AB - BCG vaccination induces a memory-like response in innate immune cells known as trained immunity. In this study, we investigated the modification of innate immune cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were examined for 7 days after TG injection by flow cytometry, and for NO production and peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage cells as following: increased in vitro LPS and BCG stimulated NO production from total cells compared to adherent cells (day 1); increased cell number (days 3 and 5); increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On day 7, cells from both experimental groups showed no production of NO in response to in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the acute phase of sterile inflammation, which may lead to the later observed phenotypic and functional changes that could be seen as accelerated resolution of inflammation and possibly point to trained immune response. PB - Wiley T1 - BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity? EP - 127 IS - S2 SP - 127 VL - 52 UR - https://hdl.handle.net/21.15107/rcub_intor_693 ER -
@article{ author = "Ćuruvija, Ivana and Blagojević, Veljko and Dragačević, Luka and Vujić, Vesna and Lukić, Ivana and Stanojević, Stanislava", year = "2022", abstract = "BCG vaccination induces a memory-like response in innate immune cells known as trained immunity. In this study, we investigated the modification of innate immune cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were examined for 7 days after TG injection by flow cytometry, and for NO production and peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage cells as following: increased in vitro LPS and BCG stimulated NO production from total cells compared to adherent cells (day 1); increased cell number (days 3 and 5); increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On day 7, cells from both experimental groups showed no production of NO in response to in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the acute phase of sterile inflammation, which may lead to the later observed phenotypic and functional changes that could be seen as accelerated resolution of inflammation and possibly point to trained immune response.", publisher = "Wiley", title = "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?", pages = "127-127", number = "S2", volume = "52", url = "https://hdl.handle.net/21.15107/rcub_intor_693" }
Ćuruvija, I., Blagojević, V., Dragačević, L., Vujić, V., Lukić, I.,& Stanojević, S.. (2022). BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. Wiley., 52(S2), 127-127. https://hdl.handle.net/21.15107/rcub_intor_693
Ćuruvija I, Blagojević V, Dragačević L, Vujić V, Lukić I, Stanojević S. BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. 2022;52(S2):127-127. https://hdl.handle.net/21.15107/rcub_intor_693 .
Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?", 52, no. S2 (2022):127-127, https://hdl.handle.net/21.15107/rcub_intor_693 .