Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4
Само за регистроване кориснике
2015
Аутори
Blagojević, VeljkoĆuruvija, Ivana
Petrović, Raisa
Vujnović, I.
Dimitrijević, Mirjana
Vujić, V.
Stanojević, Stanislava
Leposavić, Gordana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of eit...her LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.
Извор:
4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book, 2015, 341-341Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Blagojević, Veljko AU - Ćuruvija, Ivana AU - Petrović, Raisa AU - Vujnović, I. AU - Dimitrijević, Mirjana AU - Vujić, V. AU - Stanojević, Stanislava AU - Leposavić, Gordana PY - 2015 UR - http://intor.torlakinstitut.com/handle/123456789/671 AB - The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected. C3 - 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book T1 - Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4 EP - 341 SP - 341 SP - P.C.12.06 UR - https://hdl.handle.net/21.15107/rcub_intor_671 ER -
@conference{ author = "Blagojević, Veljko and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, I. and Dimitrijević, Mirjana and Vujić, V. and Stanojević, Stanislava and Leposavić, Gordana", year = "2015", abstract = "The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.", journal = "4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book", title = "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4", pages = "341-341-P.C.12.06", url = "https://hdl.handle.net/21.15107/rcub_intor_671" }
Blagojević, V., Ćuruvija, I., Petrović, R., Vujnović, I., Dimitrijević, M., Vujić, V., Stanojević, S.,& Leposavić, G.. (2015). Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book, 341-341. https://hdl.handle.net/21.15107/rcub_intor_671
Blagojević V, Ćuruvija I, Petrović R, Vujnović I, Dimitrijević M, Vujić V, Stanojević S, Leposavić G. Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book. 2015;:341-341. https://hdl.handle.net/21.15107/rcub_intor_671 .
Blagojević, Veljko, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, I., Dimitrijević, Mirjana, Vujić, V., Stanojević, Stanislava, Leposavić, Gordana, "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4" in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book (2015):341-341, https://hdl.handle.net/21.15107/rcub_intor_671 .