Potential impact of early-life probiotic supplementation on peritoneal macrophage function
Аутори
Blagojević, VeljkoPetrović, Raisa
Ćuruvija, Ivana
Prijić, Ivana
Vujić, Vesna
Stanojević, Stanislava
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser ...degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.
Кључне речи:
Lactobacillus rhamnosus / early life probiotic consumptionИзвор:
Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia, 2019, 34-Издавач:
- Belgrade: Institute for Biological Research "Siniša Stanković"
- University of Belgrade; Immunological Society of Serbia
Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Blagojević, Veljko AU - Petrović, Raisa AU - Ćuruvija, Ivana AU - Prijić, Ivana AU - Vujić, Vesna AU - Stanojević, Stanislava PY - 2019 UR - http://intor.torlakinstitut.com/handle/123456789/665 AB - Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats. PB - Belgrade: Institute for Biological Research "Siniša Stanković" PB - University of Belgrade; Immunological Society of Serbia C3 - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia T1 - Potential impact of early-life probiotic supplementation on peritoneal macrophage function SP - 34 UR - https://hdl.handle.net/21.15107/rcub_intor_665 ER -
@conference{ author = "Blagojević, Veljko and Petrović, Raisa and Ćuruvija, Ivana and Prijić, Ivana and Vujić, Vesna and Stanojević, Stanislava", year = "2019", abstract = "Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.", publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia", journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia", title = "Potential impact of early-life probiotic supplementation on peritoneal macrophage function", pages = "34", url = "https://hdl.handle.net/21.15107/rcub_intor_665" }
Blagojević, V., Petrović, R., Ćuruvija, I., Prijić, I., Vujić, V.,& Stanojević, S.. (2019). Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia Belgrade: Institute for Biological Research "Siniša Stanković"., 34. https://hdl.handle.net/21.15107/rcub_intor_665
Blagojević V, Petrović R, Ćuruvija I, Prijić I, Vujić V, Stanojević S. Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. 2019;:34. https://hdl.handle.net/21.15107/rcub_intor_665 .
Blagojević, Veljko, Petrović, Raisa, Ćuruvija, Ivana, Prijić, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Potential impact of early-life probiotic supplementation on peritoneal macrophage function" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia (2019):34, https://hdl.handle.net/21.15107/rcub_intor_665 .