Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats
Само за регистроване кориснике
2021
Аутори
Blagojević, VeljkoVujić, Vesna
Ćuruvija, Ivana
Veljović, Katarina
Soković Bajić, Svetlana
Stanojević, Stanislava
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from
day 4 to day 30). Feces were collected for microbial analysis. Colitis was induced at day 85. Seven days later rats were graded for histological damage in colon, and samples of
mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic
treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well
as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells,
attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rat...s. Our findings reveal that effects of probiotic treatment are sex‐specific to an
extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood.
The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to
be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and
severity of colitis symptoms in untreated rats.
Кључне речи:
Animal models / inflammatory bowel disease / lymphoid organs / macrophageИзвор:
European Journal of Immunology, 2021, 51, 311-311Издавач:
- Wiley
Напомена:
- Supplement: 1; Meeting Abstract: P-0690
Институција/група
TorlakTY - CONF AU - Blagojević, Veljko AU - Vujić, Vesna AU - Ćuruvija, Ivana AU - Veljović, Katarina AU - Soković Bajić, Svetlana AU - Stanojević, Stanislava PY - 2021 UR - http://intor.torlakinstitut.com/handle/123456789/667 AB - We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from day 4 to day 30). Feces were collected for microbial analysis. Colitis was induced at day 85. Seven days later rats were graded for histological damage in colon, and samples of mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and severity of colitis symptoms in untreated rats. PB - Wiley C3 - European Journal of Immunology T1 - Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats EP - 311 SP - 311 VL - 51 UR - https://hdl.handle.net/21.15107/rcub_intor_667 ER -
@conference{ author = "Blagojević, Veljko and Vujić, Vesna and Ćuruvija, Ivana and Veljović, Katarina and Soković Bajić, Svetlana and Stanojević, Stanislava", year = "2021", abstract = "We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from day 4 to day 30). Feces were collected for microbial analysis. Colitis was induced at day 85. Seven days later rats were graded for histological damage in colon, and samples of mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and severity of colitis symptoms in untreated rats.", publisher = "Wiley", journal = "European Journal of Immunology", title = "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats", pages = "311-311", volume = "51", url = "https://hdl.handle.net/21.15107/rcub_intor_667" }
Blagojević, V., Vujić, V., Ćuruvija, I., Veljović, K., Soković Bajić, S.,& Stanojević, S.. (2021). Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology Wiley., 51, 311-311. https://hdl.handle.net/21.15107/rcub_intor_667
Blagojević V, Vujić V, Ćuruvija I, Veljović K, Soković Bajić S, Stanojević S. Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology. 2021;51:311-311. https://hdl.handle.net/21.15107/rcub_intor_667 .
Blagojević, Veljko, Vujić, Vesna, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Stanojević, Stanislava, "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats" in European Journal of Immunology, 51 (2021):311-311, https://hdl.handle.net/21.15107/rcub_intor_667 .