17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner
Аутори
Ćuruvija, IvanaStanojević, Stanislava
Blagojević, Veljko
Petrović, Raisa
Prijić, Ivana
Vujić, Vesna
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aging differently affects the expression of estrogen receptors alpha and beta
(ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of
male and female rats. We explored the involvement of ERα and ERβ in the in
vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol
(which stimulates both receptors) or genistein (which is predominantly an ERβ
agonist) on inflammatory cytokine secretion from young (3 months old) and
middle-aged (16 months old) female and male AO rats. Aging diminished the
proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages
from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via
ERα, and suppressed it in cells from young females via ERβ. Genistein-induced
decrease of IL-1β in macrophages from all experimental groups was probably
mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all
experim...ental groups via ERα while genistein diminished it in all females’ and
in middle-aged male rats’ macrophages by activating ERβ. However, genistein
increased IL-6 secretion from macrophages of young male rats via ERα.
Although 17β-estradiol and genistein stimulated secretion of macrophage
inflammatory cytokines via ERα and suppressed it probably via ERβ, their
modulatory actions were determined by aging-induced changes in macrophage
ERs expression and possible ERα / ERβ interactions (Supported by Ministry of
Education, Science and Technological development, Republic of Serbia, Grant
No 175050).
Кључне речи:
estrogen receptors alpha / estrogen receptors beta / LPS-stimulated peritoneal macrophagesИзвор:
Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia., 2019, 132-132Издавач:
- Belgrade: Institute for Biological Research "Siniša Stanković"
- University of Belgrade; Immunological Society of Serbia
Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Ćuruvija, Ivana AU - Stanojević, Stanislava AU - Blagojević, Veljko AU - Petrović, Raisa AU - Prijić, Ivana AU - Vujić, Vesna PY - 2019 UR - http://intor.torlakinstitut.com/handle/123456789/664 AB - Aging differently affects the expression of estrogen receptors alpha and beta (ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of male and female rats. We explored the involvement of ERα and ERβ in the in vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol (which stimulates both receptors) or genistein (which is predominantly an ERβ agonist) on inflammatory cytokine secretion from young (3 months old) and middle-aged (16 months old) female and male AO rats. Aging diminished the proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via ERα, and suppressed it in cells from young females via ERβ. Genistein-induced decrease of IL-1β in macrophages from all experimental groups was probably mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all experimental groups via ERα while genistein diminished it in all females’ and in middle-aged male rats’ macrophages by activating ERβ. However, genistein increased IL-6 secretion from macrophages of young male rats via ERα. Although 17β-estradiol and genistein stimulated secretion of macrophage inflammatory cytokines via ERα and suppressed it probably via ERβ, their modulatory actions were determined by aging-induced changes in macrophage ERs expression and possible ERα / ERβ interactions (Supported by Ministry of Education, Science and Technological development, Republic of Serbia, Grant No 175050). PB - Belgrade: Institute for Biological Research "Siniša Stanković" PB - University of Belgrade; Immunological Society of Serbia C3 - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. T1 - 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner EP - 132 SP - 132 UR - https://hdl.handle.net/21.15107/rcub_intor_664 ER -
@conference{ author = "Ćuruvija, Ivana and Stanojević, Stanislava and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna", year = "2019", abstract = "Aging differently affects the expression of estrogen receptors alpha and beta (ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of male and female rats. We explored the involvement of ERα and ERβ in the in vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol (which stimulates both receptors) or genistein (which is predominantly an ERβ agonist) on inflammatory cytokine secretion from young (3 months old) and middle-aged (16 months old) female and male AO rats. Aging diminished the proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via ERα, and suppressed it in cells from young females via ERβ. Genistein-induced decrease of IL-1β in macrophages from all experimental groups was probably mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all experimental groups via ERα while genistein diminished it in all females’ and in middle-aged male rats’ macrophages by activating ERβ. However, genistein increased IL-6 secretion from macrophages of young male rats via ERα. Although 17β-estradiol and genistein stimulated secretion of macrophage inflammatory cytokines via ERα and suppressed it probably via ERβ, their modulatory actions were determined by aging-induced changes in macrophage ERs expression and possible ERα / ERβ interactions (Supported by Ministry of Education, Science and Technological development, Republic of Serbia, Grant No 175050).", publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia", journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.", title = "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner", pages = "132-132", url = "https://hdl.handle.net/21.15107/rcub_intor_664" }
Ćuruvija, I., Stanojević, S., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2019). 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. Belgrade: Institute for Biological Research "Siniša Stanković"., 132-132. https://hdl.handle.net/21.15107/rcub_intor_664
Ćuruvija I, Stanojević S, Blagojević V, Petrović R, Prijić I, Vujić V. 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.. 2019;:132-132. https://hdl.handle.net/21.15107/rcub_intor_664 .
Ćuruvija, Ivana, Stanojević, Stanislava, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. (2019):132-132, https://hdl.handle.net/21.15107/rcub_intor_664 .