TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/424
AB  - This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains
EP  - 1449
IS  - 4
SP  - 1434
VL  - 38
DO  - 10.1007/s10753-015-0118-0
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2015",
abstract = "This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains",
pages = "1449-1434",
number = "4",
volume = "38",
doi = "10.1007/s10753-015-0118-0"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V.,& Dimitrijević, M.. (2015). Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation
Springer/Plenum Publishers, New York., 38(4), 1434-1449.
https://doi.org/10.1007/s10753-015-0118-0

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Dimitrijević M. Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation. 2015;38(4):1434-1449.
doi:10.1007/s10753-015-0118-0 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains" in Inflammation, 38, no. 4 (2015):1434-1449,
https://doi.org/10.1007/s10753-015-0118-0 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/382
AB  - Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats
EP  - 572
IS  - 16
SP  - 564
VL  - 93
DO  - 10.1016/j.lfs.2013.08.021
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Dimitrijević, Mirjana",
year = "2013",
abstract = "Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats",
pages = "572-564",
number = "16",
volume = "93",
doi = "10.1016/j.lfs.2013.08.021"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I.,& Dimitrijević, M.. (2013). Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 93(16), 564-572.
https://doi.org/10.1016/j.lfs.2013.08.021

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Dimitrijević M. Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences. 2013;93(16):564-572.
doi:10.1016/j.lfs.2013.08.021 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Dimitrijević, Mirjana, "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats" in Life Sciences, 93, no. 16 (2013):564-572,
https://doi.org/10.1016/j.lfs.2013.08.021 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/383
AB  - The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production
EP  - 1254
IS  - 11
SP  - 1243
VL  - 48
DO  - 10.1016/j.exger.2013.07.001
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Radojević, Katarina and Leposavić, Gordana",
year = "2013",
abstract = "The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production",
pages = "1254-1243",
number = "11",
volume = "48",
doi = "10.1016/j.exger.2013.07.001"
}

Dimitrijević, M., Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I., Radojević, K.,& Leposavić, G.. (2013). The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 48(11), 1243-1254.
https://doi.org/10.1016/j.exger.2013.07.001

Dimitrijević M, Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Radojević K, Leposavić G. The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology. 2013;48(11):1243-1254.
doi:10.1016/j.exger.2013.07.001 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Radojević, Katarina, Leposavić, Gordana, "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production" in Experimental Gerontology, 48, no. 11 (2013):1243-1254,
https://doi.org/10.1016/j.exger.2013.07.001 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/389
AB  - New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.
PB  - Wiley-Blackwell, Hoboken
T2  - Experimental Physiology
T1  - Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production
EP  - 678
IS  - 3
SP  - 665
VL  - 98
DO  - 10.1113/expphysiol.2012.070524
ER  - 

@article{
author = "Stanojević, Stanislava and Dimitrijević, Mirjana and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Leposavić, Gordana",
year = "2013",
abstract = "New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Experimental Physiology",
title = "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production",
pages = "678-665",
number = "3",
volume = "98",
doi = "10.1113/expphysiol.2012.070524"
}

Stanojević, S., Dimitrijević, M., Kuštrimović, N., Mitić, K., Vujić, V.,& Leposavić, G.. (2013). Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology
Wiley-Blackwell, Hoboken., 98(3), 665-678.
https://doi.org/10.1113/expphysiol.2012.070524

Stanojević S, Dimitrijević M, Kuštrimović N, Mitić K, Vujić V, Leposavić G. Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology. 2013;98(3):665-678.
doi:10.1113/expphysiol.2012.070524 .

Stanojević, Stanislava, Dimitrijević, Mirjana, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Leposavić, Gordana, "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production" in Experimental Physiology, 98, no. 3 (2013):665-678,
https://doi.org/10.1113/expphysiol.2012.070524 . .

TY  - CONF
AU  - Aleksić, Iva
AU  - Kuštrimović, Nataša
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/963
AB  - It етра тле that KM exerts ap fics рon ит of the res immune ои: The aim of the present Study was
to clarify whether ageing influences phenotype characteristics of the resident and thioglycollate-elicited rat peritoneal macrophages
and their functional capacities.
Frequency of Е02+ cells was considerably reduced in resident peritoneal exudate cells obtained from old animals (17 months)
when compared to young (2 months) and adult animals (8 months). The expression of ED2 and CD14 molecules on ED2+ cells also
gradually decreased during ageing. In addition, LPS-elicited NO production and TNF-a were decreased in macrophages acquired from
adult and old rats, respectively.
In contrast, the frequency of ED2+ cells did not change during ageing in thioglycollate-elicited peritoneal macrophages, whereas
density of ED2 marker on these cells was markedly increased in old animals when compared to young and adult animals. Induction
of thioglycollate peritonitis also led to the rise of CD16+ cells within population of peritoneal cells in adult and old animals when
compared to young rats, while density of this marker on ED2+ cells gradually decreased during ageing. Phagocytic potential of macrophages was also decreased during ageing.
Overall, these data suggest that functional capabilities of rat macrophages are affected by senescence and that it could be a
consequence of observed age-related changes in expression of ED2, CD14 and CD16 markers on macrophages.
(Supported by Ministry of Science, Serbia, Grant 175050)
PB  - EMDS
C3  - 26th Annual EMDS Meeting 1-3 September, 2012 Debrecen, Hungary
T1  - C Role of macrophages in pathophysiology
SP  - C01
UR  - https://hdl.handle.net/21.15107/rcub_intor_963
ER  - 

@conference{
author = "Aleksić, Iva and Kuštrimović, Nataša and Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2012",
abstract = "It етра тле that KM exerts ap fics рon ит of the res immune ои: The aim of the present Study was
to clarify whether ageing influences phenotype characteristics of the resident and thioglycollate-elicited rat peritoneal macrophages
and their functional capacities.
Frequency of Е02+ cells was considerably reduced in resident peritoneal exudate cells obtained from old animals (17 months)
when compared to young (2 months) and adult animals (8 months). The expression of ED2 and CD14 molecules on ED2+ cells also
gradually decreased during ageing. In addition, LPS-elicited NO production and TNF-a were decreased in macrophages acquired from
adult and old rats, respectively.
In contrast, the frequency of ED2+ cells did not change during ageing in thioglycollate-elicited peritoneal macrophages, whereas
density of ED2 marker on these cells was markedly increased in old animals when compared to young and adult animals. Induction
of thioglycollate peritonitis also led to the rise of CD16+ cells within population of peritoneal cells in adult and old animals when
compared to young rats, while density of this marker on ED2+ cells gradually decreased during ageing. Phagocytic potential of macrophages was also decreased during ageing.
Overall, these data suggest that functional capabilities of rat macrophages are affected by senescence and that it could be a
consequence of observed age-related changes in expression of ED2, CD14 and CD16 markers on macrophages.
(Supported by Ministry of Science, Serbia, Grant 175050)",
publisher = "EMDS",
journal = "26th Annual EMDS Meeting 1-3 September, 2012 Debrecen, Hungary",
title = "C Role of macrophages in pathophysiology",
pages = "C01",
url = "https://hdl.handle.net/21.15107/rcub_intor_963"
}

Aleksić, I., Kuštrimović, N., Stanojević, S., Mitić, K., Vujić, V.,& Dimitrijević, M.. (2012). C Role of macrophages in pathophysiology. in 26th Annual EMDS Meeting 1-3 September, 2012 Debrecen, Hungary
EMDS., C01.
https://hdl.handle.net/21.15107/rcub_intor_963

Aleksić I, Kuštrimović N, Stanojević S, Mitić K, Vujić V, Dimitrijević M. C Role of macrophages in pathophysiology. in 26th Annual EMDS Meeting 1-3 September, 2012 Debrecen, Hungary. 2012;:C01.
https://hdl.handle.net/21.15107/rcub_intor_963 .

Aleksić, Iva, Kuštrimović, Nataša, Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, "C Role of macrophages in pathophysiology" in 26th Annual EMDS Meeting 1-3 September, 2012 Debrecen, Hungary (2012):C01,
https://hdl.handle.net/21.15107/rcub_intor_963 .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/357
AB  - Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions
EP  - 31
IS  - 1-2
SP  - 23
VL  - 245
DO  - 10.1016/j.jneuroim.2012.01.013
ER  - 

@article{
author = "Dimitrijević, Mirjana and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Stanojević, Stanislava",
year = "2012",
abstract = "Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions",
pages = "31-23",
number = "1-2",
volume = "245",
doi = "10.1016/j.jneuroim.2012.01.013"
}

Dimitrijević, M., Mitić, K., Kuštrimović, N., Vujić, V.,& Stanojević, S.. (2012). NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology
Elsevier, Amsterdam., 245(1-2), 23-31.
https://doi.org/10.1016/j.jneuroim.2012.01.013

Dimitrijević M, Mitić K, Kuštrimović N, Vujić V, Stanojević S. NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology. 2012;245(1-2):23-31.
doi:10.1016/j.jneuroim.2012.01.013 .

Dimitrijević, Mirjana, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Stanojević, Stanislava, "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions" in Journal of Neuroimmunology, 245, no. 1-2 (2012):23-31,
https://doi.org/10.1016/j.jneuroim.2012.01.013 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Leposavić, Gordana
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/359
AB  - Much evidence has identified a direct anatomical and functional link between the brain and the immune system, with glucocorticoids (GCs), catecholamines (CAs), and neuropeptide Y (NPY) as its end-point mediators. This suggests the important role of these mediators in immune system homeostasis and the pathogenesis of inflammatory autoimmune diseases. However, although it is clear that these mediators can modulate lymphocyte maturation and the activity of distinct immune cell types, their putative role in the pathogenesis of autoimmune disease is not yet completely understood. We have contributed to this field by discovering the influence of CAs and GCs on fine-tuning thymocyte negative selection and, in particular, by pointing to the putative CA-mediated mechanisms underlying this influence. Furthermore, we have shown that CAs are implicated in the regulation of regulatory T-cell development in the thymus. Moreover, our investigations related to macrophage biology emphasize the complex interaction between GCs, CAs and NPY in the modulation of macrophage functions and their putative significance for the pathogenesis of autoimmune inflammatory diseases.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology
EP  - 80
IS  - 1-2
SP  - 64
VL  - 52
DO  - 10.1007/s12026-012-8275-9
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Leposavić, Gordana",
year = "2012",
abstract = "Much evidence has identified a direct anatomical and functional link between the brain and the immune system, with glucocorticoids (GCs), catecholamines (CAs), and neuropeptide Y (NPY) as its end-point mediators. This suggests the important role of these mediators in immune system homeostasis and the pathogenesis of inflammatory autoimmune diseases. However, although it is clear that these mediators can modulate lymphocyte maturation and the activity of distinct immune cell types, their putative role in the pathogenesis of autoimmune disease is not yet completely understood. We have contributed to this field by discovering the influence of CAs and GCs on fine-tuning thymocyte negative selection and, in particular, by pointing to the putative CA-mediated mechanisms underlying this influence. Furthermore, we have shown that CAs are implicated in the regulation of regulatory T-cell development in the thymus. Moreover, our investigations related to macrophage biology emphasize the complex interaction between GCs, CAs and NPY in the modulation of macrophage functions and their putative significance for the pathogenesis of autoimmune inflammatory diseases.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology",
pages = "80-64",
number = "1-2",
volume = "52",
doi = "10.1007/s12026-012-8275-9"
}

Dimitrijević, M., Stanojević, S., Kuštrimović, N.,& Leposavić, G.. (2012). End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology. in Immunologic Research
Humana Press Inc, Totowa., 52(1-2), 64-80.
https://doi.org/10.1007/s12026-012-8275-9

Dimitrijević M, Stanojević S, Kuštrimović N, Leposavić G. End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology. in Immunologic Research. 2012;52(1-2):64-80.
doi:10.1007/s12026-012-8275-9 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Leposavić, Gordana, "End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology" in Immunologic Research, 52, no. 1-2 (2012):64-80,
https://doi.org/10.1007/s12026-012-8275-9 . .

TY  - CONF
AU  - Kuštrimović, Nataša
AU  - Mitić, Nataša
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/966
AB  - Na modelu akutne inflamacije šape indukovane intraplantarnim ubrizgavanjem
konkanavalina A (engl., Concanavalin A, Con A) kod pacova
pokazali smo sojne razlike u pogledu intenziteta i kinetike inflamatorne
reakcije, pri čemu su Dark Agouti (DA) pacovi razvijali jači
odgovor u odnosu na Albino Oxford (AO) pacove. Cilj ovog rada Je bio
da se utvrdi da li medijatori mastocita doprinose sojnim razlikama u
inflamaciji šape kod DA i AO pacova. Sistemska deplecija mastocita
Je izvršena sukcesivnim |. p. tretmanom rastućim dozama supstance
48/80, а ınflamacija Je indukovana 24 sata nakon poslednjeg tretmana.
Rezultati su pokazali da prethodna deplecija mastocita potencira inflamaciju
kod DA pacova tako što povećava dijametar ınflamirane šape
(meren nonijusom) | ekstravazaciju plazme (određivana metodom sa
Evans blue), ali smanjuje influks granulocita (određivan merenjem mi-
Jeloperoksidazne aktivnosti) u odnosu na kontrolne Životinje. Nasuprot
tome, kod AO pacova deplecija mastocita dovodi do supresije svih
parametara ınflamacije. Ispitivanjem fenotipa mononuklearnih ćelija
izolovanih iz inflamiranog tkiva šape u piku ınflamacije potvrđeno Je
smanjenje procenta HIS48* granulocita usled deplecije mastocita u
oba soja pacova. Takođe, tretman sa 48/80 povećava procenat ED2?
makrofaga u drenirajućim limfnim čvorovima AO ı DA pacova, а! | zastupljenost
ED2* makrofaga u tkivu inflamiranin Sapa kod AO pacova
Rezultati ukazuju da su medijatorı mastocita neophodni za razvoj ınflamatornog
edema Sape | ekstravazacyu plazme u AO, ali ne ı u DA
pacova. dok su u oba soja pacova mediator mastocita neophodni za
regrutaciju ćelija u ınflamatorni fokus ı remodelovanje ćelijskog miljea
drenirajućih limfnih čvorova u ınflamaciji.
C3  - VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija
T1  - Uloga mast ćelija u inflamaciji šape i dva inbredna soja pacova, Dark Agouti i Albino Oxford
SP  - PO6
UR  - https://hdl.handle.net/21.15107/rcub_intor_966
ER  - 

@conference{
author = "Kuštrimović, Nataša and Mitić, Nataša and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2011",
abstract = "Na modelu akutne inflamacije šape indukovane intraplantarnim ubrizgavanjem
konkanavalina A (engl., Concanavalin A, Con A) kod pacova
pokazali smo sojne razlike u pogledu intenziteta i kinetike inflamatorne
reakcije, pri čemu su Dark Agouti (DA) pacovi razvijali jači
odgovor u odnosu na Albino Oxford (AO) pacove. Cilj ovog rada Je bio
da se utvrdi da li medijatori mastocita doprinose sojnim razlikama u
inflamaciji šape kod DA i AO pacova. Sistemska deplecija mastocita
Je izvršena sukcesivnim |. p. tretmanom rastućim dozama supstance
48/80, а ınflamacija Je indukovana 24 sata nakon poslednjeg tretmana.
Rezultati su pokazali da prethodna deplecija mastocita potencira inflamaciju
kod DA pacova tako što povećava dijametar ınflamirane šape
(meren nonijusom) | ekstravazaciju plazme (određivana metodom sa
Evans blue), ali smanjuje influks granulocita (određivan merenjem mi-
Jeloperoksidazne aktivnosti) u odnosu na kontrolne Životinje. Nasuprot
tome, kod AO pacova deplecija mastocita dovodi do supresije svih
parametara ınflamacije. Ispitivanjem fenotipa mononuklearnih ćelija
izolovanih iz inflamiranog tkiva šape u piku ınflamacije potvrđeno Je
smanjenje procenta HIS48* granulocita usled deplecije mastocita u
oba soja pacova. Takođe, tretman sa 48/80 povećava procenat ED2?
makrofaga u drenirajućim limfnim čvorovima AO ı DA pacova, а! | zastupljenost
ED2* makrofaga u tkivu inflamiranin Sapa kod AO pacova
Rezultati ukazuju da su medijatorı mastocita neophodni za razvoj ınflamatornog
edema Sape | ekstravazacyu plazme u AO, ali ne ı u DA
pacova. dok su u oba soja pacova mediator mastocita neophodni za
regrutaciju ćelija u ınflamatorni fokus ı remodelovanje ćelijskog miljea
drenirajućih limfnih čvorova u ınflamaciji.",
journal = "VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija",
title = "Uloga mast ćelija u inflamaciji šape i dva inbredna soja pacova, Dark Agouti i Albino Oxford",
pages = "PO6",
url = "https://hdl.handle.net/21.15107/rcub_intor_966"
}

Kuštrimović, N., Mitić, N., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2011). Uloga mast ćelija u inflamaciji šape i dva inbredna soja pacova, Dark Agouti i Albino Oxford. in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija, PO6.
https://hdl.handle.net/21.15107/rcub_intor_966

Kuštrimović N, Mitić N, Vujić V, Dimitrijević M, Stanojević S. Uloga mast ćelija u inflamaciji šape i dva inbredna soja pacova, Dark Agouti i Albino Oxford. in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija. 2011;:PO6.
https://hdl.handle.net/21.15107/rcub_intor_966 .

Kuštrimović, Nataša, Mitić, Nataša, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Uloga mast ćelija u inflamaciji šape i dva inbredna soja pacova, Dark Agouti i Albino Oxford" in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija (2011):PO6,
https://hdl.handle.net/21.15107/rcub_intor_966 .

TY  - CONF
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/965
AB  - Poznato je da neuropeptid Y (NPY) moduliše aktivnost ćelija urođene
imunosti koje učestvuju u razvoju lokalne inflamatorne reakcije.
Efekti NPY su posredovani različitim podtipovima У receptora (Y1—5)
i zavise od aktivacije ćelija i prisustva dipeptidil peptidaze 4 (DP4),
enzima koji je odgovoran za razgradnju NPY do peptida koji nema
afinitet za Y1 receptor. Cilj rada bio је da se ispita uticaj NPY na lokalnu
inflamaciju indukovanu ubrizgavanjem lipopolisaharıda (LPS) u
dorzalni vazdušni mehur Dark Agouti (DA) pacova, kao i sposobnost
NPY da u in vitro uslovima moduliše aktivnost granulocita periferne
krvi stimulisanih LPS-om. Imajući u vidu da granulociti ispoljavaju više
podtipova Y receptora u eksperimentima su pored NPY korišćeni razliciti
agonisti i antagonisti specifični za Y1, Y2 i Y5 receptore. Rezultati
su pokazali da NPY direktno ubrizgan u dorzalni vazdušni mehur
značajno smanjuje broj ćelija na mestu inflamacije, kao i da suprimira
njihovu sposobnost adhezije (merena aktivnošću mijeloperoksidaze),
fagocitoze zimozana (merena redukcijom tetrazolijum soli) i produkci-
Je NO (merena Griessovom reakcijom). Primenom agonista i antagonista
specifičnih za pojedine podtipove Y receptora utvrđeno je da su
Y2 ı YS receptori uključeni u supresiju adhezije i produkcije МО ćelija
iz vazdušnog mehura, dok je supresija fagocitoze posredovana Y2 receptorima.
Nasuprot ovim nalazima, NPY posredstvom Y1 receptora
stimuliše sposobnost adhezije i fagocitoze granulocita periferne krvi.
Takođe je pokazano da razlike u efektima NPY in vivo i in vitro potiču
od visoke aktivnosti enzima DP4 u plazmi DA pacova.
C3  - VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija
T1  - Supresivni efekti NPY na funkcije granulocita kod pacova: uloga Y2 i У5 receptora
SP  - PO5
UR  - https://hdl.handle.net/21.15107/rcub_intor_965
ER  - 

@conference{
author = "Mitić, Katarina and Dimitrijević, Mirjana and Kuštrimović, Nataša and Vujić, Vesna and Kovačević-Jovanović, Vesna and Stanojević, Stanislava",
year = "2011",
abstract = "Poznato je da neuropeptid Y (NPY) moduliše aktivnost ćelija urođene
imunosti koje učestvuju u razvoju lokalne inflamatorne reakcije.
Efekti NPY su posredovani različitim podtipovima У receptora (Y1—5)
i zavise od aktivacije ćelija i prisustva dipeptidil peptidaze 4 (DP4),
enzima koji je odgovoran za razgradnju NPY do peptida koji nema
afinitet za Y1 receptor. Cilj rada bio је da se ispita uticaj NPY na lokalnu
inflamaciju indukovanu ubrizgavanjem lipopolisaharıda (LPS) u
dorzalni vazdušni mehur Dark Agouti (DA) pacova, kao i sposobnost
NPY da u in vitro uslovima moduliše aktivnost granulocita periferne
krvi stimulisanih LPS-om. Imajući u vidu da granulociti ispoljavaju više
podtipova Y receptora u eksperimentima su pored NPY korišćeni razliciti
agonisti i antagonisti specifični za Y1, Y2 i Y5 receptore. Rezultati
su pokazali da NPY direktno ubrizgan u dorzalni vazdušni mehur
značajno smanjuje broj ćelija na mestu inflamacije, kao i da suprimira
njihovu sposobnost adhezije (merena aktivnošću mijeloperoksidaze),
fagocitoze zimozana (merena redukcijom tetrazolijum soli) i produkci-
Je NO (merena Griessovom reakcijom). Primenom agonista i antagonista
specifičnih za pojedine podtipove Y receptora utvrđeno je da su
Y2 ı YS receptori uključeni u supresiju adhezije i produkcije МО ćelija
iz vazdušnog mehura, dok je supresija fagocitoze posredovana Y2 receptorima.
Nasuprot ovim nalazima, NPY posredstvom Y1 receptora
stimuliše sposobnost adhezije i fagocitoze granulocita periferne krvi.
Takođe je pokazano da razlike u efektima NPY in vivo i in vitro potiču
od visoke aktivnosti enzima DP4 u plazmi DA pacova.",
journal = "VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija",
title = "Supresivni efekti NPY na funkcije granulocita kod pacova: uloga Y2 i У5 receptora",
pages = "PO5",
url = "https://hdl.handle.net/21.15107/rcub_intor_965"
}

Mitić, K., Dimitrijević, M., Kuštrimović, N., Vujić, V., Kovačević-Jovanović, V.,& Stanojević, S.. (2011). Supresivni efekti NPY na funkcije granulocita kod pacova: uloga Y2 i У5 receptora. in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija, PO5.
https://hdl.handle.net/21.15107/rcub_intor_965

Mitić K, Dimitrijević M, Kuštrimović N, Vujić V, Kovačević-Jovanović V, Stanojević S. Supresivni efekti NPY na funkcije granulocita kod pacova: uloga Y2 i У5 receptora. in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija. 2011;:PO5.
https://hdl.handle.net/21.15107/rcub_intor_965 .

Mitić, Katarina, Dimitrijević, Mirjana, Kuštrimović, Nataša, Vujić, Vesna, Kovačević-Jovanović, Vesna, Stanojević, Stanislava, "Supresivni efekti NPY na funkcije granulocita kod pacova: uloga Y2 i У5 receptora" in VI Naučni sastanak Društva imunologa Srbije, Program i zbornik sažetaka, Beograd, 5. maj 2011. godine Vojnomedicinska akademija (2011):PO5,
https://hdl.handle.net/21.15107/rcub_intor_965 .

TY  - CONF
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Dimitrijević, Mirjana
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/964
AB  - The aim of the study was to clarify whether strain differences in the phenolype and function of inflammatory peritoneal exudate cells
(PEC) from two inbred rat strains, Dark Agouti (DA) and Albino Oxford (АО), might be connected to their diverse regulation by mast
cells, While thioglycollate injection enhanced proportion of granulocytes among PEC in DA rats paralleled by the increase in the PEC
phagocytosing ability and the decrease in their capacity to produce nitric oxide (NO) and tumor necrosis alpha (TNFalpha),
thioglycollate injection decreased the percentages of granulocytes in AO rats, followed by the increase in both NO and TNFalpha
production of PEC. Mast cells depletion during peritonitis in DA rats, opposite to thioglycollate alone, diminished PEC yield and ability to
produce hydrogen peroxide, and diminished proportion of granulocytes, ED1+ cells and ED2+ cells bearing H1 receptors. In contrast,
mast cell depletion during ongoing peritonitis in AO rat strain exerted mostly additive effects to thioglycollate, observed in the additional
increase of the PEC yield, phagocytosis, hydrogen peroxide, NO and TNFalpha production, and the supplementary decrease in the
percentages of peritoneal granulocytes. Thus, differences in the regulation by peritoneal mast cells might contribute to the variations in
the pentoneal inflammation of DA and AO rat strains (Supported by Ministry of Science, Serbia, Grant 175050).
PB  - EMDS
C3  - 25th Annual EMDS Meeting: Clinical and Fundamental Aspects of Monocyte, Macrophage and DC Plasticity, 22-24 September 2011 Brussels, Belgium
T1  - The effect of mast cells depletion on thioglycollate-induced peritoneal cells phenotype and function in two inbred rat strains
SP  - A01
UR  - https://hdl.handle.net/21.15107/rcub_intor_964
ER  - 

@conference{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Dimitrijević, Mirjana",
year = "2011",
abstract = "The aim of the study was to clarify whether strain differences in the phenolype and function of inflammatory peritoneal exudate cells
(PEC) from two inbred rat strains, Dark Agouti (DA) and Albino Oxford (АО), might be connected to their diverse regulation by mast
cells, While thioglycollate injection enhanced proportion of granulocytes among PEC in DA rats paralleled by the increase in the PEC
phagocytosing ability and the decrease in their capacity to produce nitric oxide (NO) and tumor necrosis alpha (TNFalpha),
thioglycollate injection decreased the percentages of granulocytes in AO rats, followed by the increase in both NO and TNFalpha
production of PEC. Mast cells depletion during peritonitis in DA rats, opposite to thioglycollate alone, diminished PEC yield and ability to
produce hydrogen peroxide, and diminished proportion of granulocytes, ED1+ cells and ED2+ cells bearing H1 receptors. In contrast,
mast cell depletion during ongoing peritonitis in AO rat strain exerted mostly additive effects to thioglycollate, observed in the additional
increase of the PEC yield, phagocytosis, hydrogen peroxide, NO and TNFalpha production, and the supplementary decrease in the
percentages of peritoneal granulocytes. Thus, differences in the regulation by peritoneal mast cells might contribute to the variations in
the pentoneal inflammation of DA and AO rat strains (Supported by Ministry of Science, Serbia, Grant 175050).",
publisher = "EMDS",
journal = "25th Annual EMDS Meeting: Clinical and Fundamental Aspects of Monocyte, Macrophage and DC Plasticity, 22-24 September 2011 Brussels, Belgium",
title = "The effect of mast cells depletion on thioglycollate-induced peritoneal cells phenotype and function in two inbred rat strains",
pages = "A01",
url = "https://hdl.handle.net/21.15107/rcub_intor_964"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I.,& Dimitrijević, M.. (2011). The effect of mast cells depletion on thioglycollate-induced peritoneal cells phenotype and function in two inbred rat strains. in 25th Annual EMDS Meeting: Clinical and Fundamental Aspects of Monocyte, Macrophage and DC Plasticity, 22-24 September 2011 Brussels, Belgium
EMDS., A01.
https://hdl.handle.net/21.15107/rcub_intor_964

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Dimitrijević M. The effect of mast cells depletion on thioglycollate-induced peritoneal cells phenotype and function in two inbred rat strains. in 25th Annual EMDS Meeting: Clinical and Fundamental Aspects of Monocyte, Macrophage and DC Plasticity, 22-24 September 2011 Brussels, Belgium. 2011;:A01.
https://hdl.handle.net/21.15107/rcub_intor_964 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Dimitrijević, Mirjana, "The effect of mast cells depletion on thioglycollate-induced peritoneal cells phenotype and function in two inbred rat strains" in 25th Annual EMDS Meeting: Clinical and Fundamental Aspects of Monocyte, Macrophage and DC Plasticity, 22-24 September 2011 Brussels, Belgium (2011):A01,
https://hdl.handle.net/21.15107/rcub_intor_964 .

TY  - CONF
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/325
PB  - Birkhauser Verlag Ag, Basel
C3  - Inflammation Research
T1  - Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells
EP  - 87
SP  - 87
VL  - 60
UR  - https://hdl.handle.net/21.15107/rcub_intor_325
ER  - 

@conference{
author = "Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2011",
publisher = "Birkhauser Verlag Ag, Basel",
journal = "Inflammation Research",
title = "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells",
pages = "87-87",
volume = "60",
url = "https://hdl.handle.net/21.15107/rcub_intor_325"
}

Kuštrimović, N., Mitić, K., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2011). Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research
Birkhauser Verlag Ag, Basel., 60, 87-87.
https://hdl.handle.net/21.15107/rcub_intor_325

Kuštrimović N, Mitić K, Vujić V, Dimitrijević M, Stanojević S. Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research. 2011;60:87-87.
https://hdl.handle.net/21.15107/rcub_intor_325 .

Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells" in Inflammation Research, 60 (2011):87-87,
https://hdl.handle.net/21.15107/rcub_intor_325 .

TY  - JOUR
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/326
AB  - The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection.
AB  - Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors
T1  - Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora
EP  - 132
IS  - 2-3
SP  - 119
VL  - 61
DO  - 10.2298/AVB1103119K
ER  - 

@article{
author = "Kuštrimović, Nataša and Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava",
year = "2011",
abstract = "The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection., Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors, Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora",
pages = "132-119",
number = "2-3",
volume = "61",
doi = "10.2298/AVB1103119K"
}

Kuštrimović, N., Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V., Miletić, T.,& Stanojević, S.. (2011). Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 119-132.
https://doi.org/10.2298/AVB1103119K

Kuštrimović N, Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Miletić T, Stanojević S. Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd. 2011;61(2-3):119-132.
doi:10.2298/AVB1103119K .

Kuštrimović, Nataša, Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors" in Acta veterinaria - Beograd, 61, no. 2-3 (2011):119-132,
https://doi.org/10.2298/AVB1103119K . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/327
AB  - Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors
EP  - 1633
IS  - 8
SP  - 1626
VL  - 32
DO  - 10.1016/j.peptides.2011.06.007
ER  - 

@article{
author = "Mitić, Katarina and Stanojević, Stanislava and Kuštrimović, Nataša and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2011",
abstract = "Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors",
pages = "1633-1626",
number = "8",
volume = "32",
doi = "10.1016/j.peptides.2011.06.007"
}

Mitić, K., Stanojević, S., Kuštrimović, N., Vujić, V.,& Dimitrijević, M.. (2011). Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides
Elsevier Science Inc, New York., 32(8), 1626-1633.
https://doi.org/10.1016/j.peptides.2011.06.007

Mitić K, Stanojević S, Kuštrimović N, Vujić V, Dimitrijević M. Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides. 2011;32(8):1626-1633.
doi:10.1016/j.peptides.2011.06.007 .

Mitić, Katarina, Stanojević, Stanislava, Kuštrimović, Nataša, Vujić, Vesna, Dimitrijević, Mirjana, "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors" in Peptides, 32, no. 8 (2011):1626-1633,
https://doi.org/10.1016/j.peptides.2011.06.007 . .

TY  - JOUR
AU  - Rakin, Ana
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Živković, Irena
AU  - Janković, I.
AU  - Mićić, Mileva
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/335
AB  - To investigate the differences between thymocytes development in vivo and in vitro, thymus lobe fragments from 12-weeks old male Albino Oxford rats were cultivated over a 7-days period. In the controls and cultivated thymic lobes fragments were evaluated and the viability, apoptosis and cell cycle of thymocytes, as well as the histological characteristics of thymic tissue. Additionally, we analyzed the expression of CD4, CD8 and TCRαβ on thymocytes by flow cytometry. The obtained results showed that thymus cellularity decreased during cultivated time due to expanded apoptosis, decreased proliferation and the absence of progenitors reseeding thymus. The relative proportion of thymocyte subsets in the first 24 hours of culture remained similar as in the control. However, cultivation for 3 and 7 days modulated the relative proportions between thymoctye subsets. The percentage of DP TCRαβlow increased, DP TCRαβhi subset remained unchanged, both SP TCRαβhi subsets decreased while the same mature SP phenotype dominated in culture media. These results demonstrate that cultivated thymic fragments retain the capacity for T cell development, although cultivation modulates this process.
AB  - Sa namerom da ispitamo razlike između in vivo i in vitro sazrevanja timocita gajili smo fragmente lobusa timusa poreklom od mužjaka Albino Oksford pacova, starih dvanaest nedelja, u vremenskom periodu od sedam dana. Nakon kultivacije određivani su vijabilnost, apoptoza i ćelijski ciklus timocita, kao i histološke osobine timusnog tkiva. Takođe je analizirano ispoljavanje markera diferentovanja CD4, CD8 and TCRαβ na površini timocita metodom tečne citofluorometrije. Dobijeni rezultati su ukazali da sedmodnevna kultivacija dovodi do smanjenja broja ćelija u timusu usled povećane apoptoze, smanjene proliferacije i odsustva ulaska progenitora timocita. Tokom prvih 24 sata kultivacije ne dolazi do promena u odnosima timocitnih populacija. Međutim, duže vreme kultivacije - 3 i 7 dana moduliše relativne odnose između timocitnih subpopulacija - povećava se procenat DP TCRαβlow, procenat DP TCRαβhi timocita ostaje nepromenjen, dok su procenti ćelija oba subseta SP TCRαβhi smanjeni, mada je prisustvo pomenutih SP subsetova dominantno u medijumu za kultivaciju. Navedeni rezultati pokazuju da kultivisani fragmenti timusnog tkiva zadržavaju sposobnost da podrže sazrevanje timocita u jednostruko pozitivne T ćelije, mada je diferentovanje timocita donekle modulisano kultivacijom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Rat thymocytes differentiation in adult thymus organ culture
T1  - Diferentovanje timocita u organ kulturi timusa odraslih pacova
EP  - 478
IS  - 5-6
SP  - 461
VL  - 61
DO  - 10.2298/AVB1106461R
ER  - 

@article{
author = "Rakin, Ana and Kuštrimović, Nataša and Kosec, Duško and Živković, Irena and Janković, I. and Mićić, Mileva",
year = "2011",
abstract = "To investigate the differences between thymocytes development in vivo and in vitro, thymus lobe fragments from 12-weeks old male Albino Oxford rats were cultivated over a 7-days period. In the controls and cultivated thymic lobes fragments were evaluated and the viability, apoptosis and cell cycle of thymocytes, as well as the histological characteristics of thymic tissue. Additionally, we analyzed the expression of CD4, CD8 and TCRαβ on thymocytes by flow cytometry. The obtained results showed that thymus cellularity decreased during cultivated time due to expanded apoptosis, decreased proliferation and the absence of progenitors reseeding thymus. The relative proportion of thymocyte subsets in the first 24 hours of culture remained similar as in the control. However, cultivation for 3 and 7 days modulated the relative proportions between thymoctye subsets. The percentage of DP TCRαβlow increased, DP TCRαβhi subset remained unchanged, both SP TCRαβhi subsets decreased while the same mature SP phenotype dominated in culture media. These results demonstrate that cultivated thymic fragments retain the capacity for T cell development, although cultivation modulates this process., Sa namerom da ispitamo razlike između in vivo i in vitro sazrevanja timocita gajili smo fragmente lobusa timusa poreklom od mužjaka Albino Oksford pacova, starih dvanaest nedelja, u vremenskom periodu od sedam dana. Nakon kultivacije određivani su vijabilnost, apoptoza i ćelijski ciklus timocita, kao i histološke osobine timusnog tkiva. Takođe je analizirano ispoljavanje markera diferentovanja CD4, CD8 and TCRαβ na površini timocita metodom tečne citofluorometrije. Dobijeni rezultati su ukazali da sedmodnevna kultivacija dovodi do smanjenja broja ćelija u timusu usled povećane apoptoze, smanjene proliferacije i odsustva ulaska progenitora timocita. Tokom prvih 24 sata kultivacije ne dolazi do promena u odnosima timocitnih populacija. Međutim, duže vreme kultivacije - 3 i 7 dana moduliše relativne odnose između timocitnih subpopulacija - povećava se procenat DP TCRαβlow, procenat DP TCRαβhi timocita ostaje nepromenjen, dok su procenti ćelija oba subseta SP TCRαβhi smanjeni, mada je prisustvo pomenutih SP subsetova dominantno u medijumu za kultivaciju. Navedeni rezultati pokazuju da kultivisani fragmenti timusnog tkiva zadržavaju sposobnost da podrže sazrevanje timocita u jednostruko pozitivne T ćelije, mada je diferentovanje timocita donekle modulisano kultivacijom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Rat thymocytes differentiation in adult thymus organ culture, Diferentovanje timocita u organ kulturi timusa odraslih pacova",
pages = "478-461",
number = "5-6",
volume = "61",
doi = "10.2298/AVB1106461R"
}

Rakin, A., Kuštrimović, N., Kosec, D., Živković, I., Janković, I.,& Mićić, M.. (2011). Rat thymocytes differentiation in adult thymus organ culture. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 461-478.
https://doi.org/10.2298/AVB1106461R

Rakin A, Kuštrimović N, Kosec D, Živković I, Janković I, Mićić M. Rat thymocytes differentiation in adult thymus organ culture. in Acta veterinaria - Beograd. 2011;61(5-6):461-478.
doi:10.2298/AVB1106461R .

Rakin, Ana, Kuštrimović, Nataša, Kosec, Duško, Živković, Irena, Janković, I., Mićić, Mileva, "Rat thymocytes differentiation in adult thymus organ culture" in Acta veterinaria - Beograd, 61, no. 5-6 (2011):461-478,
https://doi.org/10.2298/AVB1106461R . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/311
AB  - We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.
AB  - Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction
T1  - Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).
EP  - 121
IS  - 2-3
SP  - 105
VL  - 60
DO  - 10.2298/AVB1003105M
ER  - 

@article{
author = "Mitić, Katarina and Miletić, Tatjana and Kovačević-Jovanović, Vesna and Kuštrimović, Nataša and Kosec, Duško and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2010",
abstract = "We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity., Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction, Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).",
pages = "121-105",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003105M"
}

Mitić, K., Miletić, T., Kovačević-Jovanović, V., Kuštrimović, N., Kosec, D., Dimitrijević, M.,& Stanojević, S.. (2010). Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 105-121.
https://doi.org/10.2298/AVB1003105M

Mitić K, Miletić T, Kovačević-Jovanović V, Kuštrimović N, Kosec D, Dimitrijević M, Stanojević S. Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd. 2010;60(2-3):105-121.
doi:10.2298/AVB1003105M .

Mitić, Katarina, Miletić, Tatjana, Kovačević-Jovanović, Vesna, Kuštrimović, Nataša, Kosec, Duško, Dimitrijević, Mirjana, Stanojević, Stanislava, "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction" in Acta veterinaria - Beograd, 60, no. 2-3 (2010):105-121,
https://doi.org/10.2298/AVB1003105M . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/298
AB  - It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity
EP  - 109
IS  - 1-3
SP  - 100
VL  - 159
DO  - 10.1016/j.regpep.2009.11.002
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2010",
abstract = "It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity",
pages = "109-100",
number = "1-3",
volume = "159",
doi = "10.1016/j.regpep.2009.11.002"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2010). Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 159(1-3), 100-109.
https://doi.org/10.1016/j.regpep.2009.11.002

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides. 2010;159(1-3):100-109.
doi:10.1016/j.regpep.2009.11.002 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity" in Regulatory Peptides, 159, no. 1-3 (2010):100-109,
https://doi.org/10.1016/j.regpep.2009.11.002 . .

TY  - CONF
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Kosec, Duško
AU  - Dimitrijević, Mirjana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/967
AB  - Objectives: Upon the induction of experimental autoimmune encephalomyelitis (EAE), the innate immune system activation occurs prior to the expansion of
encephalitogenic T lymphocytes. Therefore, we have investigated phenotype and functions of peritoneal macrophages obtained from EAE-susceptible Dark Agouti
(DA) and EAE-resistant Albino Oxford (AO) rat strains over the time period shortly following the immunization.
Methods: On days 1, 3 and 7 post immunization resident cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyses
for the presence of ED1, ED2 and CD11b molecules, and tested for zymosan phagocytosis and hydrogen peroxide (H2O2) production.
Results: In non-immunized rats, macrophages from DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from AO strain. The immunization
induced an increase in phagocytosis in DA rats’ cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability
in DA rats was associated with significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to more pronounced expression of ED2
antigen (resident macrophages) on cells from AO rats. The immunization also increased the expression of CD11b molecule on non-resident ED2- macrophages of
DA, but not of AO rats.
Conclusion: Our results suggest that the observed early increase in macrophage phagocytosis, accompanied by a low level of H2O2 production, is permissive for
EAE induction in DA rat strain. The inability of AO rats macrophages to up-regulate both CD11b and phagocytosis, in combination with constantly augmented oxidation
state, might contribute to their resistance to the induction of EAE. The early and subtle phenotype changes in peritoneal cells of both rat strains might be
significant for the mechanism contributing to their different sensitivity to the induction of autoimmunity.
PB  - Wiley
C3  - European Journal of Immunology: Abstracts from the 2nd European Congress of Immunology
T1  - The changes in peritoneal macrophage phenotype and functions at the early stages following immunization for experimental autoimmune encephalomyelitis (EAE) reflected different susceptibility to eae induction in two rat strains
IS  - S1
SP  - PA07/94
VL  - 39
UR  - https://hdl.handle.net/21.15107/rcub_intor_967
ER  - 

@conference{
author = "Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Kosec, Duško and Dimitrijević, Mirjana",
year = "2009",
abstract = "Objectives: Upon the induction of experimental autoimmune encephalomyelitis (EAE), the innate immune system activation occurs prior to the expansion of
encephalitogenic T lymphocytes. Therefore, we have investigated phenotype and functions of peritoneal macrophages obtained from EAE-susceptible Dark Agouti
(DA) and EAE-resistant Albino Oxford (AO) rat strains over the time period shortly following the immunization.
Methods: On days 1, 3 and 7 post immunization resident cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyses
for the presence of ED1, ED2 and CD11b molecules, and tested for zymosan phagocytosis and hydrogen peroxide (H2O2) production.
Results: In non-immunized rats, macrophages from DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from AO strain. The immunization
induced an increase in phagocytosis in DA rats’ cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability
in DA rats was associated with significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to more pronounced expression of ED2
antigen (resident macrophages) on cells from AO rats. The immunization also increased the expression of CD11b molecule on non-resident ED2- macrophages of
DA, but not of AO rats.
Conclusion: Our results suggest that the observed early increase in macrophage phagocytosis, accompanied by a low level of H2O2 production, is permissive for
EAE induction in DA rat strain. The inability of AO rats macrophages to up-regulate both CD11b and phagocytosis, in combination with constantly augmented oxidation
state, might contribute to their resistance to the induction of EAE. The early and subtle phenotype changes in peritoneal cells of both rat strains might be
significant for the mechanism contributing to their different sensitivity to the induction of autoimmunity.",
publisher = "Wiley",
journal = "European Journal of Immunology: Abstracts from the 2nd European Congress of Immunology",
title = "The changes in peritoneal macrophage phenotype and functions at the early stages following immunization for experimental autoimmune encephalomyelitis (EAE) reflected different susceptibility to eae induction in two rat strains",
number = "S1",
pages = "PA07/94",
volume = "39",
url = "https://hdl.handle.net/21.15107/rcub_intor_967"
}

Stanojević, S., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Kosec, D.,& Dimitrijević, M.. (2009). The changes in peritoneal macrophage phenotype and functions at the early stages following immunization for experimental autoimmune encephalomyelitis (EAE) reflected different susceptibility to eae induction in two rat strains. in European Journal of Immunology: Abstracts from the 2nd European Congress of Immunology
Wiley., 39(S1), PA07/94.
https://hdl.handle.net/21.15107/rcub_intor_967

Stanojević S, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Kosec D, Dimitrijević M. The changes in peritoneal macrophage phenotype and functions at the early stages following immunization for experimental autoimmune encephalomyelitis (EAE) reflected different susceptibility to eae induction in two rat strains. in European Journal of Immunology: Abstracts from the 2nd European Congress of Immunology. 2009;39(S1):PA07/94.
https://hdl.handle.net/21.15107/rcub_intor_967 .

Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Kosec, Duško, Dimitrijević, Mirjana, "The changes in peritoneal macrophage phenotype and functions at the early stages following immunization for experimental autoimmune encephalomyelitis (EAE) reflected different susceptibility to eae induction in two rat strains" in European Journal of Immunology: Abstracts from the 2nd European Congress of Immunology, 39, no. S1 (2009):PA07/94,
https://hdl.handle.net/21.15107/rcub_intor_967 .

TY  - CONF
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/969
AB  - A sympathetic neurotransmitter neuropeptide Y (NPY)
plays an important role in regulation of the immune
and inflammatory responses. The pleiotropic action
of NPY is accomplished by the multiplicity of NPY
receptors, of which at least five subtypes (Y1, Y2, Y4,
Y5 and Y6) have been identified so far. In addition to
tissue-specific receptor subtype expression, NPY actions may also be regulated by its processing by dipeptidyl peptidase IV (DPIV, also known as CD26) which
cleaves NPY1-36 to an Y2/Y5 receptor preferring peptide, NPY3-36, therefore terminating its function on
Y1 receptor. The present study investigated the agedependent effect of NPY on Concanavalin A-induced
inflammatory paw edema and peritoneal macrophage
nitric oxide production in Dark Agouti rats exhibiting
a high plasma DPIV activity, as acknowledged earlier.
The receptor specificity was examined by use of NPY
related peptides with different individual pharmacological specificity for Y receptor subtypes. The results
showed that plasma DPIV activity decreased, while
macrophage DPIV activity, as well as macrophage
CD26 expression, increased with aging. NPY-induced
suppression of paw edema in adult and aged rats was
mediated via Y1 and Y5 receptors. In contrast to the
in vivo situation, NPY stimulated macrophage nitric
oxide production in vitro only in young rats, and this
effect was mediated via Y1 and Y2 receptors. It can
be concluded that age-dependant modulation of inflammatory reactions by NPY is, at least in part, determined by plasma and/or macrophage DPIV activity
at different ages. This work was supported by grant
(145049) from the Ministry of Science, Serbia.
PB  - Sciendo
C3  - Serbian Journal of Experimental and Clinical Research
T1  - The anti-inflammatory effect of neuropeptide y (NPY) in rats is dependent on dipeptidyl peptidase iv (DPIV) activity and age
EP  - 94
IS  - Supplement 1
SP  - 93
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_intor_969
ER  - 

@conference{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2008",
abstract = "A sympathetic neurotransmitter neuropeptide Y (NPY)
plays an important role in regulation of the immune
and inflammatory responses. The pleiotropic action
of NPY is accomplished by the multiplicity of NPY
receptors, of which at least five subtypes (Y1, Y2, Y4,
Y5 and Y6) have been identified so far. In addition to
tissue-specific receptor subtype expression, NPY actions may also be regulated by its processing by dipeptidyl peptidase IV (DPIV, also known as CD26) which
cleaves NPY1-36 to an Y2/Y5 receptor preferring peptide, NPY3-36, therefore terminating its function on
Y1 receptor. The present study investigated the agedependent effect of NPY on Concanavalin A-induced
inflammatory paw edema and peritoneal macrophage
nitric oxide production in Dark Agouti rats exhibiting
a high plasma DPIV activity, as acknowledged earlier.
The receptor specificity was examined by use of NPY
related peptides with different individual pharmacological specificity for Y receptor subtypes. The results
showed that plasma DPIV activity decreased, while
macrophage DPIV activity, as well as macrophage
CD26 expression, increased with aging. NPY-induced
suppression of paw edema in adult and aged rats was
mediated via Y1 and Y5 receptors. In contrast to the
in vivo situation, NPY stimulated macrophage nitric
oxide production in vitro only in young rats, and this
effect was mediated via Y1 and Y2 receptors. It can
be concluded that age-dependant modulation of inflammatory reactions by NPY is, at least in part, determined by plasma and/or macrophage DPIV activity
at different ages. This work was supported by grant
(145049) from the Ministry of Science, Serbia.",
publisher = "Sciendo",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "The anti-inflammatory effect of neuropeptide y (NPY) in rats is dependent on dipeptidyl peptidase iv (DPIV) activity and age",
pages = "94-93",
number = "Supplement 1",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_intor_969"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2008). The anti-inflammatory effect of neuropeptide y (NPY) in rats is dependent on dipeptidyl peptidase iv (DPIV) activity and age. in Serbian Journal of Experimental and Clinical Research
Sciendo., 9(Supplement 1), 93-94.
https://hdl.handle.net/21.15107/rcub_intor_969

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. The anti-inflammatory effect of neuropeptide y (NPY) in rats is dependent on dipeptidyl peptidase iv (DPIV) activity and age. in Serbian Journal of Experimental and Clinical Research. 2008;9(Supplement 1):93-94.
https://hdl.handle.net/21.15107/rcub_intor_969 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "The anti-inflammatory effect of neuropeptide y (NPY) in rats is dependent on dipeptidyl peptidase iv (DPIV) activity and age" in Serbian Journal of Experimental and Clinical Research, 9, no. Supplement 1 (2008):93-94,
https://hdl.handle.net/21.15107/rcub_intor_969 .

TY  - CONF
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/972
AB  - Our previous study revealed that rats of Dark Agouti
(DA) strain developed more pronounced inflammatory
paw edema upon intraplantar (i.pl.) administration
of 50 Bl of Concanavalin A (Con A, 400 Bg per paw)
than rats of Albino Oxford (AO) strain. The aim of the
present study was to pharmacologically define Con Ainduced paw edema and to investigate contribution of
immune- and neurally-derived mediators of inflammation to the differences observed between these rat
strains. Seven months old male DA and AO rats were
i.pl. treated with 100 Bg of antagonists specific for
different receptors: opiod (naloxone), beta and alpha
adrenergic (propranolol and phentolamine, respectively), histamine H1 and H2 (chloropyramine and
ranitidine, respectively), 5-hydroxytryptamine 5HT3
(granisetron), or calcium antagonist verapamil. Thirty
minutes later rats were i.pl. treated with 400 Bg of Con
A. Intensity of inflammation was measured by the diameter of tarso-metatarsal joints with nonius before
and 3, 6, 9, 12 and 24h after injection of Con A. Results
revealed that naloxone and granisetron suppressed development of inflammatory edema in both rat strains,
confirming involvement of opioid and 5HT3 receptors
in Con A-induced inflammation in DA and AO rats. In
contrast, propranolol, phentolamine, chloropyramine,
ranitidine and verapamil oppositelly affected Con Ainduced inflammation in DA and AO rats. It could be
concluded that difference in the expression and/or
regulation of mediators of inflammation, especially
those acting via adrenergic and histamine receptors,
as well as calcium ions, in the subcutaneous tissue
of DA and AO rats, contribute to the differences in
the inflammatory response between these rat strains.
(Supported by Ministry of Science, Belgrade, Serbia,
Grant No145049)
PB  - Sciendo
C3  - Serbian Journal of Experimental and Clinical Research
T1  - Pharmacological characterization of the concanavalin A-induced paw edema in two inbred rat strains
IS  - Supplement 1
SP  - 95
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_intor_972
ER  - 

@conference{
author = "Kuštrimović, Nataša and Mitić, Katarina and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2008",
abstract = "Our previous study revealed that rats of Dark Agouti
(DA) strain developed more pronounced inflammatory
paw edema upon intraplantar (i.pl.) administration
of 50 Bl of Concanavalin A (Con A, 400 Bg per paw)
than rats of Albino Oxford (AO) strain. The aim of the
present study was to pharmacologically define Con Ainduced paw edema and to investigate contribution of
immune- and neurally-derived mediators of inflammation to the differences observed between these rat
strains. Seven months old male DA and AO rats were
i.pl. treated with 100 Bg of antagonists specific for
different receptors: opiod (naloxone), beta and alpha
adrenergic (propranolol and phentolamine, respectively), histamine H1 and H2 (chloropyramine and
ranitidine, respectively), 5-hydroxytryptamine 5HT3
(granisetron), or calcium antagonist verapamil. Thirty
minutes later rats were i.pl. treated with 400 Bg of Con
A. Intensity of inflammation was measured by the diameter of tarso-metatarsal joints with nonius before
and 3, 6, 9, 12 and 24h after injection of Con A. Results
revealed that naloxone and granisetron suppressed development of inflammatory edema in both rat strains,
confirming involvement of opioid and 5HT3 receptors
in Con A-induced inflammation in DA and AO rats. In
contrast, propranolol, phentolamine, chloropyramine,
ranitidine and verapamil oppositelly affected Con Ainduced inflammation in DA and AO rats. It could be
concluded that difference in the expression and/or
regulation of mediators of inflammation, especially
those acting via adrenergic and histamine receptors,
as well as calcium ions, in the subcutaneous tissue
of DA and AO rats, contribute to the differences in
the inflammatory response between these rat strains.
(Supported by Ministry of Science, Belgrade, Serbia,
Grant No145049)",
publisher = "Sciendo",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Pharmacological characterization of the concanavalin A-induced paw edema in two inbred rat strains",
number = "Supplement 1",
pages = "95",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_intor_972"
}

Kuštrimović, N., Mitić, K., Kovačević-Jovanović, V., Miletić, T., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2008). Pharmacological characterization of the concanavalin A-induced paw edema in two inbred rat strains. in Serbian Journal of Experimental and Clinical Research
Sciendo., 9(Supplement 1), 95.
https://hdl.handle.net/21.15107/rcub_intor_972

Kuštrimović N, Mitić K, Kovačević-Jovanović V, Miletić T, Vujić V, Dimitrijević M, Stanojević S. Pharmacological characterization of the concanavalin A-induced paw edema in two inbred rat strains. in Serbian Journal of Experimental and Clinical Research. 2008;9(Supplement 1):95.
https://hdl.handle.net/21.15107/rcub_intor_972 .

Kuštrimović, Nataša, Mitić, Katarina, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Pharmacological characterization of the concanavalin A-induced paw edema in two inbred rat strains" in Serbian Journal of Experimental and Clinical Research, 9, no. Supplement 1 (2008):95,
https://hdl.handle.net/21.15107/rcub_intor_972 .

TY  - CONF
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/970
AB  - We have previously shown that restraint stress (RS) potentiated and suppressed paw edema in Albino Oxford
(AO) and Dark Agouti (DA) rat strains, respectively. The
objective of the present study was to investigate: (a)
the effect of RS on peritoneal macrophage functions in
these two rat strains, and (b) the involvement different
neuroendocrine mediators of stress and inflammation
in the effect of RS. Rats were subjected to RS for 2 h
and elicited peritoneal macrophages were harvested
24 h later. Cells were tested for adherence and zymosan phagocytosis in the absence or presence of 10-12
–10-6 M of antagonists specific for different receptors:
opioid (naloxone), beta and alpha adrenergic (propranolol and phentolamine, respectively), histamine H1
and H2
 (chloropyramine and ranitidine, respectively),
5-hydroxytryptamine 5HT3
 (granisetron), or calcium
antagonist verapamil. Exposure to RS diminished adherence and increased phagocytosis in macrophages
obtained from rats of DA strain, but did not influence
macrophage functions in rats of AO strain. The effect of
RS on macrophages in DA rat strain was mediated via
5HT3
 receptors and Ca++ (adherence) and 5HT3
, H1
 and
H2 receptors (phagocytosis). Besides, exposure to RS
abolished stimulating effect of opioid receptor blockade and alpha-adrenergic receptor blockade on macrophage adherence. In contrast, antagonists of opioid
and alpha- and beta- adrenergic receptors potentiated
phagocytosis in macrophages from DA rats regardless of
RS. In AO rat strain RS did not modulate macrophages’
functions, but increased their sensitivity to blockade of
H2 receptors resulting in the suppression of both adherence and phagocytosis. It can be concluded that: (a) the
effect of RS on rat macrophage functions depends on
genetic background, and (b) in vivo exposure to neuroendocrine mediators released during stress creates a
hormonal milieu that change macrophage reactivity to
mediators of inflammation. (Supported by Ministry of
Science, Belgrade, Serbia, Grant No 145049).
PB  - Sciendo
C3  - Serbian Journal of Experimental and Clinical Research
T1  - Strain differences in the effect of acute restraint stress on peritoneal macrophage activity in rats
IS  - Supplement 1
SP  - 101
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_intor_970
ER  - 

@conference{
author = "Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2008",
abstract = "We have previously shown that restraint stress (RS) potentiated and suppressed paw edema in Albino Oxford
(AO) and Dark Agouti (DA) rat strains, respectively. The
objective of the present study was to investigate: (a)
the effect of RS on peritoneal macrophage functions in
these two rat strains, and (b) the involvement different
neuroendocrine mediators of stress and inflammation
in the effect of RS. Rats were subjected to RS for 2 h
and elicited peritoneal macrophages were harvested
24 h later. Cells were tested for adherence and zymosan phagocytosis in the absence or presence of 10-12
–10-6 M of antagonists specific for different receptors:
opioid (naloxone), beta and alpha adrenergic (propranolol and phentolamine, respectively), histamine H1
and H2
 (chloropyramine and ranitidine, respectively),
5-hydroxytryptamine 5HT3
 (granisetron), or calcium
antagonist verapamil. Exposure to RS diminished adherence and increased phagocytosis in macrophages
obtained from rats of DA strain, but did not influence
macrophage functions in rats of AO strain. The effect of
RS on macrophages in DA rat strain was mediated via
5HT3
 receptors and Ca++ (adherence) and 5HT3
, H1
 and
H2 receptors (phagocytosis). Besides, exposure to RS
abolished stimulating effect of opioid receptor blockade and alpha-adrenergic receptor blockade on macrophage adherence. In contrast, antagonists of opioid
and alpha- and beta- adrenergic receptors potentiated
phagocytosis in macrophages from DA rats regardless of
RS. In AO rat strain RS did not modulate macrophages’
functions, but increased their sensitivity to blockade of
H2 receptors resulting in the suppression of both adherence and phagocytosis. It can be concluded that: (a) the
effect of RS on rat macrophage functions depends on
genetic background, and (b) in vivo exposure to neuroendocrine mediators released during stress creates a
hormonal milieu that change macrophage reactivity to
mediators of inflammation. (Supported by Ministry of
Science, Belgrade, Serbia, Grant No 145049).",
publisher = "Sciendo",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Strain differences in the effect of acute restraint stress on peritoneal macrophage activity in rats",
number = "Supplement 1",
pages = "101",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_intor_970"
}

Stanojević, S., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T., Vujić, V.,& Dimitrijević, M.. (2008). Strain differences in the effect of acute restraint stress on peritoneal macrophage activity in rats. in Serbian Journal of Experimental and Clinical Research
Sciendo., 9(Supplement 1), 101.
https://hdl.handle.net/21.15107/rcub_intor_970

Stanojević S, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Vujić V, Dimitrijević M. Strain differences in the effect of acute restraint stress on peritoneal macrophage activity in rats. in Serbian Journal of Experimental and Clinical Research. 2008;9(Supplement 1):101.
https://hdl.handle.net/21.15107/rcub_intor_970 .

Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, "Strain differences in the effect of acute restraint stress on peritoneal macrophage activity in rats" in Serbian Journal of Experimental and Clinical Research, 9, no. Supplement 1 (2008):101,
https://hdl.handle.net/21.15107/rcub_intor_970 .

TY  - CONF
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/971
AB  - There is evidence for the critical role of hydrogen peroxide
(H2O2) and the nitric oxide (NO) produced by
phagocytes in development of inflammatory processes
and autoimmune inflammatory diseases. In the present
study we have investigated both phagocytotic and
secretory activity of resident peritoneal macrophages
of two rat strains: Dark Agouti (DA), susceptible, and
Albino Oxford (AO), resistant to the induction of experimental
autoimmune encephalomyelitis (EAE).
Macrophages were obtained from rats immunized with
guinea pig spinal cord in complete Freund’s adjuvant
(GPSC/CFA) on days 1, 3 and 7 post immunization
(dpi 1, 3 and 7), as well as from non-immunized control
rats, and were tested for zymosan phagocytosis,
phorbol myristate acetate-stimulated H2O2 production
and lipopolysaccharide-stimulated NO production. Before
immunization macrophages from AO, rats demonstrated
lower phagocytosis capability and higher
H2O2 production in comparison with macrophages
from DA rats, while cells from both strains produced
comparable amounts of NO. All three macrophage
functions tested in immunized rats of AO strain were
suppressed on dpi 1 and/or dpi 3 and returned to the
control levels on dpi 7. Immunization with GPSC/CFA
did not influence H2O2 and NO production in peritoneal
macrophages from DA rats, but increased zymosan
phagocytosis on dpi 1. Taken together, our results
suggest that an early increase in macrophage phagocytosis
before the development of clinical signs of the
disease in EAE-susceptible rats, as well as an early and transient decline of macrophage functions following
immunization with GPSC/CFA in EAE-resistant rats is
associated with rat strain differences in the sensitivity
to EAE induction. (This work is supported by Ministry
of Science, Belgrade, Serbia, Grant No 145049.)
PB  - Sciendo
C3  - Serbian Journal of Experimental and Clinical Research
T1  - Strain differences in peritoneal macrophage activity in experimental autoimmune encephalomyelitis in rats
EP  - 98
IS  - Supplement 1
SP  - 97
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_intor_971
ER  - 

@conference{
author = "Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2008",
abstract = "There is evidence for the critical role of hydrogen peroxide
(H2O2) and the nitric oxide (NO) produced by
phagocytes in development of inflammatory processes
and autoimmune inflammatory diseases. In the present
study we have investigated both phagocytotic and
secretory activity of resident peritoneal macrophages
of two rat strains: Dark Agouti (DA), susceptible, and
Albino Oxford (AO), resistant to the induction of experimental
autoimmune encephalomyelitis (EAE).
Macrophages were obtained from rats immunized with
guinea pig spinal cord in complete Freund’s adjuvant
(GPSC/CFA) on days 1, 3 and 7 post immunization
(dpi 1, 3 and 7), as well as from non-immunized control
rats, and were tested for zymosan phagocytosis,
phorbol myristate acetate-stimulated H2O2 production
and lipopolysaccharide-stimulated NO production. Before
immunization macrophages from AO, rats demonstrated
lower phagocytosis capability and higher
H2O2 production in comparison with macrophages
from DA rats, while cells from both strains produced
comparable amounts of NO. All three macrophage
functions tested in immunized rats of AO strain were
suppressed on dpi 1 and/or dpi 3 and returned to the
control levels on dpi 7. Immunization with GPSC/CFA
did not influence H2O2 and NO production in peritoneal
macrophages from DA rats, but increased zymosan
phagocytosis on dpi 1. Taken together, our results
suggest that an early increase in macrophage phagocytosis
before the development of clinical signs of the
disease in EAE-susceptible rats, as well as an early and transient decline of macrophage functions following
immunization with GPSC/CFA in EAE-resistant rats is
associated with rat strain differences in the sensitivity
to EAE induction. (This work is supported by Ministry
of Science, Belgrade, Serbia, Grant No 145049.)",
publisher = "Sciendo",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Strain differences in peritoneal macrophage activity in experimental autoimmune encephalomyelitis in rats",
pages = "98-97",
number = "Supplement 1",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_intor_971"
}

Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2008). Strain differences in peritoneal macrophage activity in experimental autoimmune encephalomyelitis in rats. in Serbian Journal of Experimental and Clinical Research
Sciendo., 9(Supplement 1), 97-98.
https://hdl.handle.net/21.15107/rcub_intor_971

Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Vujić V, Dimitrijević M, Stanojević S. Strain differences in peritoneal macrophage activity in experimental autoimmune encephalomyelitis in rats. in Serbian Journal of Experimental and Clinical Research. 2008;9(Supplement 1):97-98.
https://hdl.handle.net/21.15107/rcub_intor_971 .

Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Strain differences in peritoneal macrophage activity in experimental autoimmune encephalomyelitis in rats" in Serbian Journal of Experimental and Clinical Research, 9, no. Supplement 1 (2008):97-98,
https://hdl.handle.net/21.15107/rcub_intor_971 .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/260
AB  - Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress
EP  - 116
IS  - 2
SP  - 108
VL  - 15
DO  - 10.1159/000148193
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Miletić, Tatjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2008",
abstract = "Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress",
pages = "116-108",
number = "2",
volume = "15",
doi = "10.1159/000148193"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Miletić, T., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2008). The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation
Karger, Basel., 15(2), 108-116.
https://doi.org/10.1159/000148193

Stanojević S, Kuštrimović N, Mitić K, Miletić T, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation. 2008;15(2):108-116.
doi:10.1159/000148193 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Miletić, Tatjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress" in Neuroimmunomodulation, 15, no. 2 (2008):108-116,
https://doi.org/10.1159/000148193 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/259
AB  - We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.
PB  - Churchill Livingstone, Edinburgh
T2  - Neuropeptides
T1  - Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors
EP  - 158
IS  - 2
SP  - 147
VL  - 42
DO  - 10.1016/j.npep.2007.12.004
ER  - 

@article{
author = "Stanojević, Stanislava and Vujić, Vesna and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Dimitrijević, Mirjana",
year = "2008",
abstract = "We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Neuropeptides",
title = "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors",
pages = "158-147",
number = "2",
volume = "42",
doi = "10.1016/j.npep.2007.12.004"
}

Stanojević, S., Vujić, V., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T.,& Dimitrijević, M.. (2008). Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides
Churchill Livingstone, Edinburgh., 42(2), 147-158.
https://doi.org/10.1016/j.npep.2007.12.004

Stanojević S, Vujić V, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Dimitrijević M. Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides. 2008;42(2):147-158.
doi:10.1016/j.npep.2007.12.004 .

Stanojević, Stanislava, Vujić, Vesna, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Dimitrijević, Mirjana, "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors" in Neuropeptides, 42, no. 2 (2008):147-158,
https://doi.org/10.1016/j.npep.2007.12.004 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/243
AB  - Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age
EP  - 2187
IS  - 12
SP  - 2179
VL  - 29
DO  - 10.1016/j.peptides.2008.08.017
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2008",
abstract = "Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age",
pages = "2187-2179",
number = "12",
volume = "29",
doi = "10.1016/j.peptides.2008.08.017"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2008). The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides
Elsevier Science Inc, New York., 29(12), 2179-2187.
https://doi.org/10.1016/j.peptides.2008.08.017

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides. 2008;29(12):2179-2187.
doi:10.1016/j.peptides.2008.08.017 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age" in Peptides, 29, no. 12 (2008):2179-2187,
https://doi.org/10.1016/j.peptides.2008.08.017 . .

TY  - CONF
AU  - Mitić, Nataša
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/952
AB  - relevance of peripheral macrophage activity for the
susceptibility to the induction of experimental allergic
encephalomyelitis (EAE). Rats of EAE-susceptible Dark
Agouti and EAE-resistant Albino Oxford strain were
immunized with guinea pig spinal cord homogenate
(DAGPSC and AOGPSC), while non-immunized rats
served as controls (DANIM, AONIM). On day 15 after
immunization rat peritoneal macrophages were tested for
adherence capacity, zymosan phagocytosis and respiratory
burst. Macrophages from AONIM rats exhibited
lower adherence capacity and higher phagocytosis and
H2O2 production then macrophages from DANIM rats.
Immunization decreased adherence and phagocytosis and
increased H2O2 production in macrophages from AO
rats, but did not influence these activities in macrophages
from DA rats. Our results suggest that inflammatory
activities of macrophages from AO rats could be considered
as regulatory mechanisms connected with the
resistance to EAE induction (Supported by Ministry of
Science and Environmental Protection, Republic of
Serbia, Grant No 145049).
PB  - Springer
C3  - Inflammation Research (IR)
T1  - Strain differences in peritoneal macrophage activity and susceptibility to experimental allergic encephalomyelitis induction in rats
IS  - Suppl 3
SP  - S454/P09.01
VL  - 56
DO  - 10.1007/BF03353884
ER  - 

@conference{
author = "Mitić, Nataša and Stanojević, Stanislava and Kuštrimović, Nataša and Dimitrijević, Mirjana",
year = "2007",
abstract = "relevance of peripheral macrophage activity for the
susceptibility to the induction of experimental allergic
encephalomyelitis (EAE). Rats of EAE-susceptible Dark
Agouti and EAE-resistant Albino Oxford strain were
immunized with guinea pig spinal cord homogenate
(DAGPSC and AOGPSC), while non-immunized rats
served as controls (DANIM, AONIM). On day 15 after
immunization rat peritoneal macrophages were tested for
adherence capacity, zymosan phagocytosis and respiratory
burst. Macrophages from AONIM rats exhibited
lower adherence capacity and higher phagocytosis and
H2O2 production then macrophages from DANIM rats.
Immunization decreased adherence and phagocytosis and
increased H2O2 production in macrophages from AO
rats, but did not influence these activities in macrophages
from DA rats. Our results suggest that inflammatory
activities of macrophages from AO rats could be considered
as regulatory mechanisms connected with the
resistance to EAE induction (Supported by Ministry of
Science and Environmental Protection, Republic of
Serbia, Grant No 145049).",
publisher = "Springer",
journal = "Inflammation Research (IR)",
title = "Strain differences in peritoneal macrophage activity and susceptibility to experimental allergic encephalomyelitis induction in rats",
number = "Suppl 3",
pages = "S454/P09.01",
volume = "56",
doi = "10.1007/BF03353884"
}

Mitić, N., Stanojević, S., Kuštrimović, N.,& Dimitrijević, M.. (2007). Strain differences in peritoneal macrophage activity and susceptibility to experimental allergic encephalomyelitis induction in rats. in Inflammation Research (IR)
Springer., 56(Suppl 3), S454/P09.01.
https://doi.org/10.1007/BF03353884

Mitić N, Stanojević S, Kuštrimović N, Dimitrijević M. Strain differences in peritoneal macrophage activity and susceptibility to experimental allergic encephalomyelitis induction in rats. in Inflammation Research (IR). 2007;56(Suppl 3):S454/P09.01.
doi:10.1007/BF03353884 .

Mitić, Nataša, Stanojević, Stanislava, Kuštrimović, Nataša, Dimitrijević, Mirjana, "Strain differences in peritoneal macrophage activity and susceptibility to experimental allergic encephalomyelitis induction in rats" in Inflammation Research (IR), 56, no. Suppl 3 (2007):S454/P09.01,
https://doi.org/10.1007/BF03353884 . .