TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/733
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/732
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Novović, Katarina
AU  - Di Luca, Mariagrazia
AU  - Jovčić, Branko
AU  - Fira, Đorđe
AU  - Neve, Horst
AU  - Kojić, Milan
AU  - McAuliffe, Olivia
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/721
AB  - Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Medicine
T1  - Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii
VL  - 7
DO  - 10.3389/fmed.2020.00426
ER  - 

@article{
author = "Vukotić, Goran and Obradović, Mina and Novović, Katarina and Di Luca, Mariagrazia and Jovčić, Branko and Fira, Đorđe and Neve, Horst and Kojić, Milan and McAuliffe, Olivia",
year = "2020",
abstract = "Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Medicine",
title = "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii",
volume = "7",
doi = "10.3389/fmed.2020.00426"
}

Vukotić, G., Obradović, M., Novović, K., Di Luca, M., Jovčić, B., Fira, Đ., Neve, H., Kojić, M.,& McAuliffe, O.. (2020). Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmed.2020.00426

Vukotić G, Obradović M, Novović K, Di Luca M, Jovčić B, Fira Đ, Neve H, Kojić M, McAuliffe O. Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine. 2020;7.
doi:10.3389/fmed.2020.00426 .

Vukotić, Goran, Obradović, Mina, Novović, Katarina, Di Luca, Mariagrazia, Jovčić, Branko, Fira, Đorđe, Neve, Horst, Kojić, Milan, McAuliffe, Olivia, "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii" in Frontiers in Medicine, 7 (2020),
https://doi.org/10.3389/fmed.2020.00426 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Jovanović, Sofija
AU  - O'Connor, Paula M.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Dinić, Miroslav
AU  - Studholme, David John
AU  - Fira, Đorđe
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1264
UR  - http://intor.torlakinstitut.com/handle/123456789/702
AB  - Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials
IS  - 5
VL  - 14
DO  - 10.1371/journal.pone.0216773
ER  - 

@article{
author = "Miljković, Marija and Jovanović, Sofija and O'Connor, Paula M. and Mirković, Nemanja and Jovčić, Branko and Filipić, Brankica and Dinić, Miroslav and Studholme, David John and Fira, Đorđe and Cotter, Paul D. and Kojić, Milan",
year = "2019",
abstract = "Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials",
number = "5",
volume = "14",
doi = "10.1371/journal.pone.0216773"
}

Miljković, M., Jovanović, S., O'Connor, P. M., Mirković, N., Jovčić, B., Filipić, B., Dinić, M., Studholme, D. J., Fira, Đ., Cotter, P. D.,& Kojić, M.. (2019). Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One
Public Library Science, San Francisco., 14(5).
https://doi.org/10.1371/journal.pone.0216773

Miljković M, Jovanović S, O'Connor PM, Mirković N, Jovčić B, Filipić B, Dinić M, Studholme DJ, Fira Đ, Cotter PD, Kojić M. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One. 2019;14(5).
doi:10.1371/journal.pone.0216773 .

Miljković, Marija, Jovanović, Sofija, O'Connor, Paula M., Mirković, Nemanja, Jovčić, Branko, Filipić, Brankica, Dinić, Miroslav, Studholme, David John, Fira, Đorđe, Cotter, Paul D., Kojić, Milan, "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials" in PLoS One, 14, no. 5 (2019),
https://doi.org/10.1371/journal.pone.0216773 . .

TY  - JOUR
AU  - Berić, Tanja
AU  - Stanković, Slaviša
AU  - Draganić, V.
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Fira, Đorđe
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/792
UR  - http://intor.torlakinstitut.com/handle/123456789/837
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample
EP  - 510
IS  - 3
SP  - 502
VL  - 116
DO  - 10.1111/jam.12393
ER  - 

@article{
author = "Berić, Tanja and Stanković, Slaviša and Draganić, V. and Kojić, Milan and Lozo, Jelena and Fira, Đorđe",
year = "2014",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample",
pages = "510-502",
number = "3",
volume = "116",
doi = "10.1111/jam.12393"
}

Berić, T., Stanković, S., Draganić, V., Kojić, M., Lozo, J.,& Fira, Đ.. (2014). Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample. in Journal of Applied Microbiology
Wiley, Hoboken., 116(3), 502-510.
https://doi.org/10.1111/jam.12393

Berić T, Stanković S, Draganić V, Kojić M, Lozo J, Fira Đ. Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample. in Journal of Applied Microbiology. 2014;116(3):502-510.
doi:10.1111/jam.12393 .

Berić, Tanja, Stanković, Slaviša, Draganić, V., Kojić, Milan, Lozo, Jelena, Fira, Đorđe, "Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample" in Journal of Applied Microbiology, 116, no. 3 (2014):502-510,
https://doi.org/10.1111/jam.12393 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Mihajlović, Sanja
AU  - Uzelac, Gordana
AU  - Golić, Nataša
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/751
AB  - The aim of this study was to identify and characterize the lactic acid bacteria (LAB) of artisanal Golija raw and cooked cows' milk cheeses traditionally manufactured without the addition of starter culture. A total of 188 Gram-positive and catalase-negative isolates of Golija cheeses were obtained from seven samples of different ripening time. Phenotype-based assays as well as rep-PCR and 16S rDNA sequence analysis were undertaken for all 188 LAB strains. The most diverse species were isolated from 20-day-old BGGO8 cheese (Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus casei/paracasei, Lactobacillus sucicola, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis by. diacetylactis, Enterococcus faecium, Enterococcus durans and Leuconostoc mesenteroides). In other Golija cheeses Lactobacillus reuteri, Lactobacillus curvatus, Lactobacillus rhamnosus, Lactococcus lactis subsp. cremoris, Lactococcus garvieae, Streptococcus thermophilus and Leuconostoc pseudomesenteroides were found. Pronounced antimicrobial properties showed enterococci (13/42) and lactococci (12/31), while the good proteolytic activity demonstrated lactococci (13/31) and lactobacilli (10/29).
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows' milk cheeses
EP  - 192
IS  - 1
SP  - 179
VL  - 66
DO  - 10.2298/ABS1401179T
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Mihajlović, Sanja and Uzelac, Gordana and Golić, Nataša and Fira, Đorđe and Kojić, Milan and Topisirović, Ljubiša",
year = "2014",
abstract = "The aim of this study was to identify and characterize the lactic acid bacteria (LAB) of artisanal Golija raw and cooked cows' milk cheeses traditionally manufactured without the addition of starter culture. A total of 188 Gram-positive and catalase-negative isolates of Golija cheeses were obtained from seven samples of different ripening time. Phenotype-based assays as well as rep-PCR and 16S rDNA sequence analysis were undertaken for all 188 LAB strains. The most diverse species were isolated from 20-day-old BGGO8 cheese (Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus casei/paracasei, Lactobacillus sucicola, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis by. diacetylactis, Enterococcus faecium, Enterococcus durans and Leuconostoc mesenteroides). In other Golija cheeses Lactobacillus reuteri, Lactobacillus curvatus, Lactobacillus rhamnosus, Lactococcus lactis subsp. cremoris, Lactococcus garvieae, Streptococcus thermophilus and Leuconostoc pseudomesenteroides were found. Pronounced antimicrobial properties showed enterococci (13/42) and lactococci (12/31), while the good proteolytic activity demonstrated lactococci (13/31) and lactobacilli (10/29).",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows' milk cheeses",
pages = "192-179",
number = "1",
volume = "66",
doi = "10.2298/ABS1401179T"
}

Terzić-Vidojević, A., Mihajlović, S., Uzelac, G., Golić, N., Fira, Đ., Kojić, M.,& Topisirović, L.. (2014). Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows' milk cheeses. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 179-192.
https://doi.org/10.2298/ABS1401179T

Terzić-Vidojević A, Mihajlović S, Uzelac G, Golić N, Fira Đ, Kojić M, Topisirović L. Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows' milk cheeses. in Archives of Biological Sciences. 2014;66(1):179-192.
doi:10.2298/ABS1401179T .

Terzić-Vidojević, Amarela, Mihajlović, Sanja, Uzelac, Gordana, Golić, Nataša, Fira, Đorđe, Kojić, Milan, Topisirović, Ljubiša, "Identification and characterization of lactic acid bacteria isolated from artisanal white brined Golija cows' milk cheeses" in Archives of Biological Sciences, 66, no. 1 (2014):179-192,
https://doi.org/10.2298/ABS1401179T . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Golić, Nataša
AU  - Begović, Jelena
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/632
UR  - http://intor.torlakinstitut.com/handle/123456789/834
AB  - Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus
VL  - 4
DO  - 10.3389/fmicb.2013.00002
ER  - 

@article{
author = "Strahinić, Ivana and Lozo, Jelena and Terzić-Vidojević, Amarela and Fira, Đorđe and Kojić, Milan and Golić, Nataša and Begović, Jelena and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus",
volume = "4",
doi = "10.3389/fmicb.2013.00002"
}

Strahinić, I., Lozo, J., Terzić-Vidojević, A., Fira, Đ., Kojić, M., Golić, N., Begović, J.,& Topisirović, L.. (2013). Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 4.
https://doi.org/10.3389/fmicb.2013.00002

Strahinić I, Lozo J, Terzić-Vidojević A, Fira Đ, Kojić M, Golić N, Begović J, Topisirović L. Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology. 2013;4.
doi:10.3389/fmicb.2013.00002 .

Strahinić, Ivana, Lozo, Jelena, Terzić-Vidojević, Amarela, Fira, Đorđe, Kojić, Milan, Golić, Nataša, Begović, Jelena, Topisirović, Ljubiša, "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus" in Frontiers in Microbiology, 4 (2013),
https://doi.org/10.3389/fmicb.2013.00002 . .

TY  - JOUR
AU  - Ivanović, Zarko
AU  - Stanković, Slaviša
AU  - Zivković, Svetlana
AU  - Gavrilović, Veljko
AU  - Kojić, Milan
AU  - Fira, Đorđe
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/564
UR  - http://intor.torlakinstitut.com/handle/123456789/828
AB  - Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia
EP  - 203
IS  - 1
SP  - 191
VL  - 134
DO  - 10.1007/s10658-012-9978-4
ER  - 

@article{
author = "Ivanović, Zarko and Stanković, Slaviša and Zivković, Svetlana and Gavrilović, Veljko and Kojić, Milan and Fira, Đorđe",
year = "2012",
abstract = "Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia",
pages = "203-191",
number = "1",
volume = "134",
doi = "10.1007/s10658-012-9978-4"
}

Ivanović, Z., Stanković, S., Zivković, S., Gavrilović, V., Kojić, M.,& Fira, Đ.. (2012). Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 134(1), 191-203.
https://doi.org/10.1007/s10658-012-9978-4

Ivanović Z, Stanković S, Zivković S, Gavrilović V, Kojić M, Fira Đ. Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology. 2012;134(1):191-203.
doi:10.1007/s10658-012-9978-4 .

Ivanović, Zarko, Stanković, Slaviša, Zivković, Svetlana, Gavrilović, Veljko, Kojić, Milan, Fira, Đorđe, "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia" in European Journal of Plant Pathology, 134, no. 1 (2012):191-203,
https://doi.org/10.1007/s10658-012-9978-4 . .

TY  - JOUR
AU  - Tolinački, Maja
AU  - Lozo, Jelena
AU  - Veljović, Katarina
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/556
UR  - http://intor.torlakinstitut.com/handle/123456789/830
AB  - Cilj ove studije je izučavanje antimikrobnog potencijala 52 prirodna izolata vrste L. casei/paracasei. Učestalost gena koji kodiraju BacSJ (bacSJ2-8/bacSJ2-8i genski klaster), acidocin 8912 (acdT), ABC-transporter (abcT) i pomoćni protein (acc) su takođe izučavani. Genski klaster bacSJ2-8/bacSJ2-8i prisutan je kod 49 (94.23%), a acdT kod 41 (78.85%) od 52 testirana soja. Četrdeset sojeva (76.92%) poseduje oba analizirana gena. Interesantno je da samo 17 sojeva (32.69%) koji poseduju bacSJ2-8/bacSJ2-8i genski klaster i/ili acdT gen proizvode bakteriocine. Soj L. paracasei BGNK1-62 poseduje bacSJ2-8/bacSJ2-8i genski klaster, ali ne proizvodi bakteriocin BacSJ što je verovatno posledica nedostatka abcT i acc gena. Nakon transformacije soja BGNK1-62 konstruktom pA2A koji poseduje abcT i acc gene ostvarena je proizvodnja bakteriocina BacSJ. Osim toga, utvrđeno je da soj L. paracasei BGGR2-66 proizvodi nov bakteriocin označen kao BacGR, koji je biohemijski okarakterisan, a određena je i njegova N-terminalna sekvenca.
AB  - The aim of this study was to investigate the antimicrobial potential of 52 natural isolates of Lactobacillus casei/paracasei. The incidence of relevant genes encoding BacSJ (bacSJ2-8/bacSJ2-8i gene cluster), acidocin 8912 (acdT), ABC-transporter (abcT) and accessory protein (acc) was also studied. These genes were found to be widespread amongst the analyzed L. casei/paracasei strains. The bacSJ2-8/bacSJ2-8i gene cluster was present in 49 (94.23%) and acdT in 41 (78.85%) of the 52 tested strains. Forty of these strains (76.92%) harbored both analyzed genes. Interestingly, only 17 strains (32.69%) with the bacSJ2-8/bacSJ2-8i gene cluster and/or the acdT gene showed bacteriocin production. Strain L. paracasei BGNK1-62 contained the bacSJ2-8/bacSJ2-8i gene cluster, but did not produce bacteriocin BacSJ possibly due to absence of the abcT and acc genes. Hence, these genes were introduced into BGNK1-62 by transformation with constructed plasmid pA2A, after which BacSJ was produced. In addition, it was found that L. paracasei BGGR2-66 produced new bacteriocin designated as BacGR that was biochemically characterized and its N- terminal sequence was determined.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe
T1  - Examination of antimicrobial potential in natural isolates of lactobacillus casei/paracasei group
EP  - 677
IS  - 3
SP  - 661
VL  - 44
DO  - 10.2298/GENSR1203661T
ER  - 

@article{
author = "Tolinački, Maja and Lozo, Jelena and Veljović, Katarina and Kojić, Milan and Fira, Đorđe and Topisirović, Ljubiša",
year = "2012",
abstract = "Cilj ove studije je izučavanje antimikrobnog potencijala 52 prirodna izolata vrste L. casei/paracasei. Učestalost gena koji kodiraju BacSJ (bacSJ2-8/bacSJ2-8i genski klaster), acidocin 8912 (acdT), ABC-transporter (abcT) i pomoćni protein (acc) su takođe izučavani. Genski klaster bacSJ2-8/bacSJ2-8i prisutan je kod 49 (94.23%), a acdT kod 41 (78.85%) od 52 testirana soja. Četrdeset sojeva (76.92%) poseduje oba analizirana gena. Interesantno je da samo 17 sojeva (32.69%) koji poseduju bacSJ2-8/bacSJ2-8i genski klaster i/ili acdT gen proizvode bakteriocine. Soj L. paracasei BGNK1-62 poseduje bacSJ2-8/bacSJ2-8i genski klaster, ali ne proizvodi bakteriocin BacSJ što je verovatno posledica nedostatka abcT i acc gena. Nakon transformacije soja BGNK1-62 konstruktom pA2A koji poseduje abcT i acc gene ostvarena je proizvodnja bakteriocina BacSJ. Osim toga, utvrđeno je da soj L. paracasei BGGR2-66 proizvodi nov bakteriocin označen kao BacGR, koji je biohemijski okarakterisan, a određena je i njegova N-terminalna sekvenca., The aim of this study was to investigate the antimicrobial potential of 52 natural isolates of Lactobacillus casei/paracasei. The incidence of relevant genes encoding BacSJ (bacSJ2-8/bacSJ2-8i gene cluster), acidocin 8912 (acdT), ABC-transporter (abcT) and accessory protein (acc) was also studied. These genes were found to be widespread amongst the analyzed L. casei/paracasei strains. The bacSJ2-8/bacSJ2-8i gene cluster was present in 49 (94.23%) and acdT in 41 (78.85%) of the 52 tested strains. Forty of these strains (76.92%) harbored both analyzed genes. Interestingly, only 17 strains (32.69%) with the bacSJ2-8/bacSJ2-8i gene cluster and/or the acdT gene showed bacteriocin production. Strain L. paracasei BGNK1-62 contained the bacSJ2-8/bacSJ2-8i gene cluster, but did not produce bacteriocin BacSJ possibly due to absence of the abcT and acc genes. Hence, these genes were introduced into BGNK1-62 by transformation with constructed plasmid pA2A, after which BacSJ was produced. In addition, it was found that L. paracasei BGGR2-66 produced new bacteriocin designated as BacGR that was biochemically characterized and its N- terminal sequence was determined.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe, Examination of antimicrobial potential in natural isolates of lactobacillus casei/paracasei group",
pages = "677-661",
number = "3",
volume = "44",
doi = "10.2298/GENSR1203661T"
}

Tolinački, M., Lozo, J., Veljović, K., Kojić, M., Fira, Đ.,& Topisirović, L.. (2012). Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 44(3), 661-677.
https://doi.org/10.2298/GENSR1203661T

Tolinački M, Lozo J, Veljović K, Kojić M, Fira Đ, Topisirović L. Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe. in Genetika-Belgrade. 2012;44(3):661-677.
doi:10.2298/GENSR1203661T .

Tolinački, Maja, Lozo, Jelena, Veljović, Katarina, Kojić, Milan, Fira, Đorđe, Topisirović, Ljubiša, "Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe" in Genetika-Belgrade, 44, no. 3 (2012):661-677,
https://doi.org/10.2298/GENSR1203661T . .

TY  - JOUR
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Topisirović, Ljubiša
AU  - Degrassi, Giuliano
AU  - Myers, Michael
AU  - Venturi, Vittorio
AU  - Fira, Đorđe
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/574
UR  - http://intor.torlakinstitut.com/handle/123456789/691
AB  - Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria
EP  - 31
IS  - 1
SP  - 25
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_imagine_574
ER  - 

@article{
author = "Berić, Tanja and Kojić, Milan and Stanković, Slaviša and Topisirović, Ljubiša and Degrassi, Giuliano and Myers, Michael and Venturi, Vittorio and Fira, Đorđe",
year = "2012",
abstract = "Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria",
pages = "31-25",
number = "1",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_imagine_574"
}

Berić, T., Kojić, M., Stanković, S., Topisirović, L., Degrassi, G., Myers, M., Venturi, V.,& Fira, Đ.. (2012). Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology
University of Zagreb., 50(1), 25-31.
https://hdl.handle.net/21.15107/rcub_imagine_574

Berić T, Kojić M, Stanković S, Topisirović L, Degrassi G, Myers M, Venturi V, Fira Đ. Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology. 2012;50(1):25-31.
https://hdl.handle.net/21.15107/rcub_imagine_574 .

Berić, Tanja, Kojić, Milan, Stanković, Slaviša, Topisirović, Ljubiša, Degrassi, Giuliano, Myers, Michael, Venturi, Vittorio, Fira, Đorđe, "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria" in Food Technology and Biotechnology, 50, no. 1 (2012):25-31,
https://hdl.handle.net/21.15107/rcub_imagine_574 .

TY  - CHAP
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Golić, Nataša
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/519
UR  - http://intor.torlakinstitut.com/handle/123456789/682
PB  - CABI Publishing
T2  - Natural Antimicrobials in Food Safety and Quality
T1  - A survey of antimicrobial activity in lactic acid bacteria of different origin
EP  - 38
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_intor_682
ER  - 

@inbook{
author = "Topisirović, Ljubiša and Kojić, Milan and Strahinić, Ivana and Fira, Đorđe and Golić, Nataša",
year = "2011",
publisher = "CABI Publishing",
journal = "Natural Antimicrobials in Food Safety and Quality",
booktitle = "A survey of antimicrobial activity in lactic acid bacteria of different origin",
pages = "38-27",
url = "https://hdl.handle.net/21.15107/rcub_intor_682"
}

Topisirović, L., Kojić, M., Strahinić, I., Fira, Đ.,& Golić, N.. (2011). A survey of antimicrobial activity in lactic acid bacteria of different origin. in Natural Antimicrobials in Food Safety and Quality
CABI Publishing., 27-38.
https://hdl.handle.net/21.15107/rcub_intor_682

Topisirović L, Kojić M, Strahinić I, Fira Đ, Golić N. A survey of antimicrobial activity in lactic acid bacteria of different origin. in Natural Antimicrobials in Food Safety and Quality. 2011;:27-38.
https://hdl.handle.net/21.15107/rcub_intor_682 .

Topisirović, Ljubiša, Kojić, Milan, Strahinić, Ivana, Fira, Đorđe, Golić, Nataša, "A survey of antimicrobial activity in lactic acid bacteria of different origin" in Natural Antimicrobials in Food Safety and Quality (2011):27-38,
https://hdl.handle.net/21.15107/rcub_intor_682 .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Kojić, Milan
AU  - Tolinački, Maja
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/465
UR  - http://intor.torlakinstitut.com/handle/123456789/825
AB  - Aims: The study of proteolytic activity and examination of proteinase gene region organization in proteolytically active Lactobacillus plantarum strains from different natural sources. Methods and Results: A set of 37 lactobacilli was distinguished by using multiplex PCR assay. Results showed that 34 strains were Lact. plantarum and three of them were Lact. paraplantarum. The examination of proteolytic activity revealed that 28 Lact. plantarum and two Lact. paraplantarum hydrolyse beta-casein. Further analyses of all proteolytically active Lact. plantarum with primers specific for different types of CEPs demonstrated that strain BGSJ3-18 has prtP catalytic domain as well as prtP-prtM intergenic region showing more than 95% sequence identity with the same regions present in Lact. paracasei, Lact. casei and L. lactis. No presence of prtB, prtH or prtR proteinase genes was detected in any of tested Lact. plantarum strains. Conclusions: One out of 28 analysed Lact. plantarum strains harbours the prtP-like gene. The other proteolytically active Lact. plantarum probably possesses a different type of extracellular proteinase(s). Significance and Impact of the Study: It is the first report about the presence of the prtP-like gene in Lact. plantarum, which illustrates the mobility of this gene and its presence in different species.
PB  - Wiley, Hoboken
T2  - Letters in Applied Microbiology
T1  - The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18
EP  - 49
IS  - 1
SP  - 43
VL  - 50
DO  - 10.1111/j.1472-765X.2009.02748.x
ER  - 

@article{
author = "Strahinić, Ivana and Kojić, Milan and Tolinački, Maja and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "Aims: The study of proteolytic activity and examination of proteinase gene region organization in proteolytically active Lactobacillus plantarum strains from different natural sources. Methods and Results: A set of 37 lactobacilli was distinguished by using multiplex PCR assay. Results showed that 34 strains were Lact. plantarum and three of them were Lact. paraplantarum. The examination of proteolytic activity revealed that 28 Lact. plantarum and two Lact. paraplantarum hydrolyse beta-casein. Further analyses of all proteolytically active Lact. plantarum with primers specific for different types of CEPs demonstrated that strain BGSJ3-18 has prtP catalytic domain as well as prtP-prtM intergenic region showing more than 95% sequence identity with the same regions present in Lact. paracasei, Lact. casei and L. lactis. No presence of prtB, prtH or prtR proteinase genes was detected in any of tested Lact. plantarum strains. Conclusions: One out of 28 analysed Lact. plantarum strains harbours the prtP-like gene. The other proteolytically active Lact. plantarum probably possesses a different type of extracellular proteinase(s). Significance and Impact of the Study: It is the first report about the presence of the prtP-like gene in Lact. plantarum, which illustrates the mobility of this gene and its presence in different species.",
publisher = "Wiley, Hoboken",
journal = "Letters in Applied Microbiology",
title = "The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18",
pages = "49-43",
number = "1",
volume = "50",
doi = "10.1111/j.1472-765X.2009.02748.x"
}

Strahinić, I., Kojić, M., Tolinački, M., Fira, Đ.,& Topisirović, L.. (2010). The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18. in Letters in Applied Microbiology
Wiley, Hoboken., 50(1), 43-49.
https://doi.org/10.1111/j.1472-765X.2009.02748.x

Strahinić I, Kojić M, Tolinački M, Fira Đ, Topisirović L. The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18. in Letters in Applied Microbiology. 2010;50(1):43-49.
doi:10.1111/j.1472-765X.2009.02748.x .

Strahinić, Ivana, Kojić, Milan, Tolinački, Maja, Fira, Đorđe, Topisirović, Ljubiša, "The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18" in Letters in Applied Microbiology, 50, no. 1 (2010):43-49,
https://doi.org/10.1111/j.1472-765X.2009.02748.x . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/432
UR  - http://intor.torlakinstitut.com/handle/123456789/727
AB  - A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli. Lactobacillus and Lactococcus strains. It showed high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L paracasei subsp. paracasei BGSJ2-8 was cloned into pA13 using BamHI to obtain the construct, pB5. Sequencing and in silico analysis of pB5 revealed fifteen open reading frames (ORF). Plasmid pSJ2-8 harbours genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. Combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled determination of the primary structure of bacteriocin BacSJ. The bacSJ2-8 gene encodes 68-amino-acid peptide with a double-glycine leader peptide consisting of 18 amino acids, followed by the orf2 (bacSJ2-8i) which encodes the immunity protein of BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirements for production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to class II bacteriocins.
PB  - Elsevier, Amsterdam
T2  - International Journal of Food Microbiology
T1  - Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8
EP  - 124
IS  - 2-3
SP  - 117
VL  - 140
DO  - 10.1016/j.ijfoodmicro.2010.04.010
ER  - 

@article{
author = "Kojić, Milan and Lozo, Jelena and Jovčić, Branko and Strahinić, Ivana and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli. Lactobacillus and Lactococcus strains. It showed high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L paracasei subsp. paracasei BGSJ2-8 was cloned into pA13 using BamHI to obtain the construct, pB5. Sequencing and in silico analysis of pB5 revealed fifteen open reading frames (ORF). Plasmid pSJ2-8 harbours genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. Combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled determination of the primary structure of bacteriocin BacSJ. The bacSJ2-8 gene encodes 68-amino-acid peptide with a double-glycine leader peptide consisting of 18 amino acids, followed by the orf2 (bacSJ2-8i) which encodes the immunity protein of BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirements for production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to class II bacteriocins.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8",
pages = "124-117",
number = "2-3",
volume = "140",
doi = "10.1016/j.ijfoodmicro.2010.04.010"
}

Kojić, M., Lozo, J., Jovčić, B., Strahinić, I., Fira, Đ.,& Topisirović, L.. (2010). Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8. in International Journal of Food Microbiology
Elsevier, Amsterdam., 140(2-3), 117-124.
https://doi.org/10.1016/j.ijfoodmicro.2010.04.010

Kojić M, Lozo J, Jovčić B, Strahinić I, Fira Đ, Topisirović L. Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8. in International Journal of Food Microbiology. 2010;140(2-3):117-124.
doi:10.1016/j.ijfoodmicro.2010.04.010 .

Kojić, Milan, Lozo, Jelena, Jovčić, Branko, Strahinić, Ivana, Fira, Đorđe, Topisirović, Ljubiša, "Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8" in International Journal of Food Microbiology, 140, no. 2-3 (2010):117-124,
https://doi.org/10.1016/j.ijfoodmicro.2010.04.010 . .

TY  - JOUR
AU  - Tolinački, Maja
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Topisirović, Ljubiša
AU  - Fira, Đorđe
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/719
AB  - The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9
EP  - 899
IS  - 4
SP  - 889
VL  - 62
DO  - 10.2298/ABS1004889T
ER  - 

@article{
author = "Tolinački, Maja and Kojić, Milan and Lozo, Jelena and Terzić-Vidojević, Amarela and Topisirović, Ljubiša and Fira, Đorđe",
year = "2010",
abstract = "The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9",
pages = "899-889",
number = "4",
volume = "62",
doi = "10.2298/ABS1004889T"
}

Tolinački, M., Kojić, M., Lozo, J., Terzić-Vidojević, A., Topisirović, L.,& Fira, Đ.. (2010). Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 889-899.
https://doi.org/10.2298/ABS1004889T

Tolinački M, Kojić M, Lozo J, Terzić-Vidojević A, Topisirović L, Fira Đ. Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences. 2010;62(4):889-899.
doi:10.2298/ABS1004889T .

Tolinački, Maja, Kojić, Milan, Lozo, Jelena, Terzić-Vidojević, Amarela, Topisirović, Ljubiša, Fira, Đorđe, "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9" in Archives of Biological Sciences, 62, no. 4 (2010):889-899,
https://doi.org/10.2298/ABS1004889T . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/681
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Retraction notice to: Kojić, M.; Lozo, J.; Jovčić, B.; Strahinić, I.; Fira, Đ.; Topisirović, L. A Successful Use of a New Shuttle Cloning Vector pA13 for the Cloning of the Bacteriocins BacSJ and Acidocin 8912. Archives of Biological Sciences 2010, 62 (2), 231–243. https://doi.org/10.2298/ABS1002231K.
EP  - 349
IS  - 2
SP  - 349
VL  - 62
DO  - 10.2298/ABS1004251U
ER  - 

@article{
author = "Kojić, Milan and Lozo, Jelena and Jovčić, Branko and Strahinić, Ivana and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Retraction notice to: Kojić, M.; Lozo, J.; Jovčić, B.; Strahinić, I.; Fira, Đ.; Topisirović, L. A Successful Use of a New Shuttle Cloning Vector pA13 for the Cloning of the Bacteriocins BacSJ and Acidocin 8912. Archives of Biological Sciences 2010, 62 (2), 231–243. https://doi.org/10.2298/ABS1002231K.",
pages = "349-349",
number = "2",
volume = "62",
doi = "10.2298/ABS1004251U"
}

Kojić, M., Lozo, J., Jovčić, B., Strahinić, I., Fira, Đ.,& Topisirović, L.. (2010). Retraction notice to: Kojić, M.; Lozo, J.; Jovčić, B.; Strahinić, I.; Fira, Đ.; Topisirović, L. A Successful Use of a New Shuttle Cloning Vector pA13 for the Cloning of the Bacteriocins BacSJ and Acidocin 8912. Archives of Biological Sciences 2010, 62 (2), 231–243. https://doi.org/10.2298/ABS1002231K.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 349-349.
https://doi.org/10.2298/ABS1004251U

Kojić M, Lozo J, Jovčić B, Strahinić I, Fira Đ, Topisirović L. Retraction notice to: Kojić, M.; Lozo, J.; Jovčić, B.; Strahinić, I.; Fira, Đ.; Topisirović, L. A Successful Use of a New Shuttle Cloning Vector pA13 for the Cloning of the Bacteriocins BacSJ and Acidocin 8912. Archives of Biological Sciences 2010, 62 (2), 231–243. https://doi.org/10.2298/ABS1002231K.. in Archives of Biological Sciences. 2010;62(2):349-349.
doi:10.2298/ABS1004251U .

Kojić, Milan, Lozo, Jelena, Jovčić, Branko, Strahinić, Ivana, Fira, Đorđe, Topisirović, Ljubiša, "Retraction notice to: Kojić, M.; Lozo, J.; Jovčić, B.; Strahinić, I.; Fira, Đ.; Topisirović, L. A Successful Use of a New Shuttle Cloning Vector pA13 for the Cloning of the Bacteriocins BacSJ and Acidocin 8912. Archives of Biological Sciences 2010, 62 (2), 231–243. https://doi.org/10.2298/ABS1002231K." in Archives of Biological Sciences, 62, no. 2 (2010):349-349,
https://doi.org/10.2298/ABS1004251U . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/421
UR  - http://intor.torlakinstitut.com/handle/123456789/680
AB  - The aim of this paper was to research the molecular cloning of genes encoding the novel bacteriocin BacSJ from Lactobacillus paracasei subsp. paracasei BGSJ2-8 by using a newly constructed shuttle cloning vector pA13. A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli, Lactobacillus and Lactococcus strains, showing a high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L. paracasei subsp. paracasei BGSJ2-8 was cloned in the pA13 using BamHI, obtaining the construct pB5. Sequencing and in silico analysis of the pB5 revealed 15 open reading frames (ORF). Plasmid pSJ2-8 harbors the genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. The combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled the determination of the primary structure of a bacteriocin BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirement for the production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to the class II bacteriocins. The shuttle cloning vector pA13 could be used as a tool for genetic manipulations in lactobacilli and lactococci.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912
EP  - 243
IS  - 2
SP  - 231
VL  - 62
DO  - 10.2298/ABS1002231K
ER  - 

@article{
author = "Kojić, Milan and Lozo, Jelena and Jovčić, Branko and Strahinić, Ivana and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "The aim of this paper was to research the molecular cloning of genes encoding the novel bacteriocin BacSJ from Lactobacillus paracasei subsp. paracasei BGSJ2-8 by using a newly constructed shuttle cloning vector pA13. A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli, Lactobacillus and Lactococcus strains, showing a high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L. paracasei subsp. paracasei BGSJ2-8 was cloned in the pA13 using BamHI, obtaining the construct pB5. Sequencing and in silico analysis of the pB5 revealed 15 open reading frames (ORF). Plasmid pSJ2-8 harbors the genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. The combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled the determination of the primary structure of a bacteriocin BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirement for the production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to the class II bacteriocins. The shuttle cloning vector pA13 could be used as a tool for genetic manipulations in lactobacilli and lactococci.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912",
pages = "243-231",
number = "2",
volume = "62",
doi = "10.2298/ABS1002231K"
}

Kojić, M., Lozo, J., Jovčić, B., Strahinić, I., Fira, Đ.,& Topisirović, L.. (2010). A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 231-243.
https://doi.org/10.2298/ABS1002231K

Kojić M, Lozo J, Jovčić B, Strahinić I, Fira Đ, Topisirović L. A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912. in Archives of Biological Sciences. 2010;62(2):231-243.
doi:10.2298/ABS1002231K .

Kojić, Milan, Lozo, Jelena, Jovčić, Branko, Strahinić, Ivana, Fira, Đorđe, Topisirović, Ljubiša, "A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912" in Archives of Biological Sciences, 62, no. 2 (2010):231-243,
https://doi.org/10.2298/ABS1002231K . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Kojić, Milan
AU  - Tolinački, Maja
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/821
AB  - Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors
EP  - 88
IS  - 1
SP  - 78
VL  - 106
DO  - 10.1111/j.1365-2672.2008.03977.x
ER  - 

@article{
author = "Strahinić, Ivana and Kojić, Milan and Tolinački, Maja and Fira, Đorđe and Topisirović, Ljubiša",
year = "2009",
abstract = "Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors",
pages = "88-78",
number = "1",
volume = "106",
doi = "10.1111/j.1365-2672.2008.03977.x"
}

Strahinić, I., Kojić, M., Tolinački, M., Fira, Đ.,& Topisirović, L.. (2009). Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology
Wiley, Hoboken., 106(1), 78-88.
https://doi.org/10.1111/j.1365-2672.2008.03977.x

Strahinić I, Kojić M, Tolinački M, Fira Đ, Topisirović L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology. 2009;106(1):78-88.
doi:10.1111/j.1365-2672.2008.03977.x .

Strahinić, Ivana, Kojić, Milan, Tolinački, Maja, Fira, Đorđe, Topisirović, Ljubiša, "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors" in Journal of Applied Microbiology, 106, no. 1 (2009):78-88,
https://doi.org/10.1111/j.1365-2672.2008.03977.x . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Begović, Jelena
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/819
AB  - A large chromosomal inversion that confers resistance to high concentrations of the antibiotic spectinomycin in Lactococcus lactis subsp. lactis bv. diacetylactis S50 was identified by pulsed field gel electrophoresis. The same type of inversion was identified in 4 independent experiments and in 4 different derivatives of strain S50, indicating the same position and the same mechanism of recombination as a response to antibiotic selective pressure in all derivatives. An analysis of ribosomal operons in strain S50 and mutants revealed that ribosomal operons are not endpoints of the recombination. Spectinomycin-resistant mutants appeared in a population of S50 derivatives at a high frequency of 2 x 10(-7). These spectinomycin-resistant mutants were not able to compete successfully with the wild-type strain during 25 generations (48 h) of co-culture in vitro, indicating that inversion had a significant fitness cost. Results demonstrate that as a mechanism of genome plasticity, inversion can be directly involved in one-step development of the adaptation to a high concentration of spectinomycin.
PB  - Natl Research Council Canada-N R C Research Press, Ottawa
T2  - Canadian Journal of Microbiology
T1  - Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50
EP  - 149
IS  - 2
SP  - 143
VL  - 54
DO  - 10.1139/W07-121
ER  - 

@article{
author = "Kojić, Milan and Jovčić, Branko and Begović, Jelena and Fira, Đorđe and Topisirović, Ljubiša",
year = "2008",
abstract = "A large chromosomal inversion that confers resistance to high concentrations of the antibiotic spectinomycin in Lactococcus lactis subsp. lactis bv. diacetylactis S50 was identified by pulsed field gel electrophoresis. The same type of inversion was identified in 4 independent experiments and in 4 different derivatives of strain S50, indicating the same position and the same mechanism of recombination as a response to antibiotic selective pressure in all derivatives. An analysis of ribosomal operons in strain S50 and mutants revealed that ribosomal operons are not endpoints of the recombination. Spectinomycin-resistant mutants appeared in a population of S50 derivatives at a high frequency of 2 x 10(-7). These spectinomycin-resistant mutants were not able to compete successfully with the wild-type strain during 25 generations (48 h) of co-culture in vitro, indicating that inversion had a significant fitness cost. Results demonstrate that as a mechanism of genome plasticity, inversion can be directly involved in one-step development of the adaptation to a high concentration of spectinomycin.",
publisher = "Natl Research Council Canada-N R C Research Press, Ottawa",
journal = "Canadian Journal of Microbiology",
title = "Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50",
pages = "149-143",
number = "2",
volume = "54",
doi = "10.1139/W07-121"
}

Kojić, M., Jovčić, B., Begović, J., Fira, Đ.,& Topisirović, L.. (2008). Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology
Natl Research Council Canada-N R C Research Press, Ottawa., 54(2), 143-149.
https://doi.org/10.1139/W07-121

Kojić M, Jovčić B, Begović J, Fira Đ, Topisirović L. Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology. 2008;54(2):143-149.
doi:10.1139/W07-121 .

Kojić, Milan, Jovčić, Branko, Begović, Jelena, Fira, Đorđe, Topisirović, Ljubiša, "Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50" in Canadian Journal of Microbiology, 54, no. 2 (2008):143-149,
https://doi.org/10.1139/W07-121 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Cvetanović, D.
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Tolinački, Maja
AU  - Topisirović, Ljubiša
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/815
AB  - Soj Lactococcus lactis subsp. lactis BGSM1-19, izolovan iz sira tradicionalno proizvedenog u domaćinstvu, sintetiše dva bakteriocina: bakteriocin BacSMa koji pripada grupi laktokokcina B i bakteriocin BacSMb koji pokazuje visoku homologiju sa lakticinom RM. Čišćenjem plazmida, sa relativno niskim prinosom (0,33%), dobijene su dve grupe derivata: BacSMa- BacSMas derivat i BacSMa- BacSMas, BacSMb-, BacSMbs.. Sinteza bakteriocina je ispitivana tokom logaritamske faze rasta pri čemu je utvrđen maksimum proizvodnje u kulturi staroj 8 sati gajenoj na temperaturi od 30oC, što odgovara ranoj stacionarnoj fazi rasta. Biohemijska karakterizacija je ukazala da soj BGSM1-19 zadržava antimikrobnu aktivnost u opsegu pH vrednosti od 1 do 12 kao i posle tretmana na 100 oC u trajanju od 15 minuta. Utvrđeno je da se antimikrobna aktivnost potpuno gubi nakon tretmana različitim proteolitičkim enzimima. Soj BGSM1-19 poseduje pet plazmida. U eksperimentima čišćenja od plazmida utvrđeno je da se geni za sintezu i imunost na bakteriocine nalaze na plazmidima. Pored toga, BGSM1-19 pokazuje antimikrobnu aktivnost na ispitivane patogene bakterije kao što su Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa i Staphylococcus aureus.
AB  - The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33%) of BacSMa- BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Karakterizacija i antimikrobna aktivnost prirodnog izolata Lactococcus lactis subsp. lactis BGSM1-19
T1  - Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19
EP  - 521
IS  - 5-6
SP  - 509
VL  - 57
DO  - 10.2298/AVB0706509S
ER  - 

@article{
author = "Strahinić, Ivana and Cvetanović, D. and Kojić, Milan and Fira, Đorđe and Tolinački, Maja and Topisirović, Ljubiša",
year = "2007",
abstract = "Soj Lactococcus lactis subsp. lactis BGSM1-19, izolovan iz sira tradicionalno proizvedenog u domaćinstvu, sintetiše dva bakteriocina: bakteriocin BacSMa koji pripada grupi laktokokcina B i bakteriocin BacSMb koji pokazuje visoku homologiju sa lakticinom RM. Čišćenjem plazmida, sa relativno niskim prinosom (0,33%), dobijene su dve grupe derivata: BacSMa- BacSMas derivat i BacSMa- BacSMas, BacSMb-, BacSMbs.. Sinteza bakteriocina je ispitivana tokom logaritamske faze rasta pri čemu je utvrđen maksimum proizvodnje u kulturi staroj 8 sati gajenoj na temperaturi od 30oC, što odgovara ranoj stacionarnoj fazi rasta. Biohemijska karakterizacija je ukazala da soj BGSM1-19 zadržava antimikrobnu aktivnost u opsegu pH vrednosti od 1 do 12 kao i posle tretmana na 100 oC u trajanju od 15 minuta. Utvrđeno je da se antimikrobna aktivnost potpuno gubi nakon tretmana različitim proteolitičkim enzimima. Soj BGSM1-19 poseduje pet plazmida. U eksperimentima čišćenja od plazmida utvrđeno je da se geni za sintezu i imunost na bakteriocine nalaze na plazmidima. Pored toga, BGSM1-19 pokazuje antimikrobnu aktivnost na ispitivane patogene bakterije kao što su Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa i Staphylococcus aureus., The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33%) of BacSMa- BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Karakterizacija i antimikrobna aktivnost prirodnog izolata Lactococcus lactis subsp. lactis BGSM1-19, Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19",
pages = "521-509",
number = "5-6",
volume = "57",
doi = "10.2298/AVB0706509S"
}

Strahinić, I., Cvetanović, D., Kojić, M., Fira, Đ., Tolinački, M.,& Topisirović, L.. (2007). Karakterizacija i antimikrobna aktivnost prirodnog izolata Lactococcus lactis subsp. lactis BGSM1-19. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 509-521.
https://doi.org/10.2298/AVB0706509S

Strahinić I, Cvetanović D, Kojić M, Fira Đ, Tolinački M, Topisirović L. Karakterizacija i antimikrobna aktivnost prirodnog izolata Lactococcus lactis subsp. lactis BGSM1-19. in Acta Veterinaria-Beograd. 2007;57(5-6):509-521.
doi:10.2298/AVB0706509S .

Strahinić, Ivana, Cvetanović, D., Kojić, Milan, Fira, Đorđe, Tolinački, Maja, Topisirović, Ljubiša, "Karakterizacija i antimikrobna aktivnost prirodnog izolata Lactococcus lactis subsp. lactis BGSM1-19" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):509-521,
https://doi.org/10.2298/AVB0706509S . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Topisirović, Ljubiša
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/810
AB  - In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains produced two additional bacteriocins (LsbA and LsbB) with genes responsible for their production and immunity located on the small rolling circle-replicating plasmid pMN5. Using deletion experiments of pMN5, minimal replicon of the plasmid and involvement of a bacteriocin locus in plasmid maintenance were identified. In addition, plasmid curing experiments showed that genes for catabolism or transport of 10 carbohydrates in the strain BGMN1-5 were plasmid located.
PB  - Canadian Science Publishing, Ottawa
T2  - Canadian Journal of Microbiology
T1  - Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese
EP  - 1120
IS  - 11
SP  - 1110
VL  - 52
DO  - 10.1139/W06-072
ER  - 

@article{
author = "Kojić, Milan and Strahinić, Ivana and Fira, Đorđe and Jovčić, Branko and Topisirović, Ljubiša",
year = "2006",
abstract = "In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains produced two additional bacteriocins (LsbA and LsbB) with genes responsible for their production and immunity located on the small rolling circle-replicating plasmid pMN5. Using deletion experiments of pMN5, minimal replicon of the plasmid and involvement of a bacteriocin locus in plasmid maintenance were identified. In addition, plasmid curing experiments showed that genes for catabolism or transport of 10 carbohydrates in the strain BGMN1-5 were plasmid located.",
publisher = "Canadian Science Publishing, Ottawa",
journal = "Canadian Journal of Microbiology",
title = "Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese",
pages = "1120-1110",
number = "11",
volume = "52",
doi = "10.1139/W06-072"
}

Kojić, M., Strahinić, I., Fira, Đ., Jovčić, B.,& Topisirović, L.. (2006). Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese. in Canadian Journal of Microbiology
Canadian Science Publishing, Ottawa., 52(11), 1110-1120.
https://doi.org/10.1139/W06-072

Kojić M, Strahinić I, Fira Đ, Jovčić B, Topisirović L. Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese. in Canadian Journal of Microbiology. 2006;52(11):1110-1120.
doi:10.1139/W06-072 .

Kojić, Milan, Strahinić, Ivana, Fira, Đorđe, Jovčić, Branko, Topisirović, Ljubiša, "Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese" in Canadian Journal of Microbiology, 52, no. 11 (2006):1110-1120,
https://doi.org/10.1139/W06-072 . .

TY  - CONF
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Golić, Nataša
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/811
AB  - Autochthonous strains of lactic acid bacteria (LAB) have been isolated from traditionally homemade cheeses collected from specific ecological localities across Serbia and Montenegro. Genetic and biochemical analysis of this LAB revealed that they produce bacteriocins, proteinases and exopolysaccharides. LAB produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. Apart from the metabolic end products, some strains also secrete antimicrobial substances known as bacteriocins. Among the natural isolates of LAB from homemade cheeses, bacteriocin producers were found in both lactococci and lactobacilli. Lactococcus lactis subsp. lactis BGMN1-5 was found to produce three narrow spectrum class II heat-stable bacteriocins. In addition to bacteriocin production, BGMN1-5 synthesized a cell envelope-associated protemase (CEP) and shows an aggregation phenotype. Another isolate, L. lactis subsp. lactis BGSM1-19 produces low molecular mass (7 kDa) bacteriocin SM19 that showed antimicrobial activity against Staphylococcus aureus, Micrococcus,flavus and partially against Salmonella paratyphi. Production of bacteriocin reaches a plateau after 8 h of BGSM1-19 growth. Bacteriocin SM19 retained activity within the wide pH range from I to 12 and after the treatment at 100 degrees C for 15 min. Among collection of lactobacilli, the isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces heat-stable bacteriocin SJ (approx. 5 kDa) polypeptide. It retained activity after treatment for 1 h at 100 degrees C, and in the pH range from 2 to 11. In addition to isolates from cheeses, bacteriocin-producing human oral lactobacilli were detected. Most of them showed antimicrobial activity against streptococci, staphylococci and micrococci, but not against Candida. Isolate BGHO1 that showed the highest antimicrobial activity was determined as L. paracasei. Interestingly, Lactobacillus helveticus BGRA43, which was isolated from the human intestine showed strong activity against Clostridium sporogenes, but it was not possible to detect any bacteriocin production in this isolate by using standard procedures. Further analysis of antimicrobial activity revealed that BGRA43 has a relatively broad spectrum. Lactobacilli resistant to nisin were also detected among natural isolates. They produce bacteriocins, which have no activity against nisin producing lactococci.
PB  - Elsevier Science Bv, Amsterdam
C3  - International Journal of Food Microbiology
T1  - Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation
EP  - 235
IS  - 3
SP  - 230
VL  - 112
DO  - 10.1016/j.ijfoodmicro.2006.04.009
ER  - 

@conference{
author = "Topisirović, Ljubiša and Kojić, Milan and Fira, Đorđe and Golić, Nataša and Strahinić, Ivana and Lozo, Jelena",
year = "2006",
abstract = "Autochthonous strains of lactic acid bacteria (LAB) have been isolated from traditionally homemade cheeses collected from specific ecological localities across Serbia and Montenegro. Genetic and biochemical analysis of this LAB revealed that they produce bacteriocins, proteinases and exopolysaccharides. LAB produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. Apart from the metabolic end products, some strains also secrete antimicrobial substances known as bacteriocins. Among the natural isolates of LAB from homemade cheeses, bacteriocin producers were found in both lactococci and lactobacilli. Lactococcus lactis subsp. lactis BGMN1-5 was found to produce three narrow spectrum class II heat-stable bacteriocins. In addition to bacteriocin production, BGMN1-5 synthesized a cell envelope-associated protemase (CEP) and shows an aggregation phenotype. Another isolate, L. lactis subsp. lactis BGSM1-19 produces low molecular mass (7 kDa) bacteriocin SM19 that showed antimicrobial activity against Staphylococcus aureus, Micrococcus,flavus and partially against Salmonella paratyphi. Production of bacteriocin reaches a plateau after 8 h of BGSM1-19 growth. Bacteriocin SM19 retained activity within the wide pH range from I to 12 and after the treatment at 100 degrees C for 15 min. Among collection of lactobacilli, the isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces heat-stable bacteriocin SJ (approx. 5 kDa) polypeptide. It retained activity after treatment for 1 h at 100 degrees C, and in the pH range from 2 to 11. In addition to isolates from cheeses, bacteriocin-producing human oral lactobacilli were detected. Most of them showed antimicrobial activity against streptococci, staphylococci and micrococci, but not against Candida. Isolate BGHO1 that showed the highest antimicrobial activity was determined as L. paracasei. Interestingly, Lactobacillus helveticus BGRA43, which was isolated from the human intestine showed strong activity against Clostridium sporogenes, but it was not possible to detect any bacteriocin production in this isolate by using standard procedures. Further analysis of antimicrobial activity revealed that BGRA43 has a relatively broad spectrum. Lactobacilli resistant to nisin were also detected among natural isolates. They produce bacteriocins, which have no activity against nisin producing lactococci.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation",
pages = "235-230",
number = "3",
volume = "112",
doi = "10.1016/j.ijfoodmicro.2006.04.009"
}

Topisirović, L., Kojić, M., Fira, Đ., Golić, N., Strahinić, I.,& Lozo, J.. (2006). Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. in International Journal of Food Microbiology
Elsevier Science Bv, Amsterdam., 112(3), 230-235.
https://doi.org/10.1016/j.ijfoodmicro.2006.04.009

Topisirović L, Kojić M, Fira Đ, Golić N, Strahinić I, Lozo J. Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. in International Journal of Food Microbiology. 2006;112(3):230-235.
doi:10.1016/j.ijfoodmicro.2006.04.009 .

Topisirović, Ljubiša, Kojić, Milan, Fira, Đorđe, Golić, Nataša, Strahinić, Ivana, Lozo, Jelena, "Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation" in International Journal of Food Microbiology, 112, no. 3 (2006):230-235,
https://doi.org/10.1016/j.ijfoodmicro.2006.04.009 . .

TY  - JOUR
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Banina, Ana
AU  - Spasojević, I
AU  - Strahinić, Ivana
AU  - Topisirović, Ljubiša
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/715
AB  - The proteolytic activities of two natural isolates of thermophilic lactobacilli, Lactobacillus acidophilus BGRA43 and Lact, delbrueckii BGPF1, and Lact. acidophilus CH2 (Chr. Hansen's strain) and Lact, acidophilus V74 (Visby's strain), were compared. Results revealed that optimal pH for all four proteinases is 6.5, whereas temperature optimum varied among proteinases. Determination of caseinolytic activity done under optimal conditions for each strain revealed that the CH2 and V74 proteinases completely hydrolysed both alpha (s1)-casein and beta -casein, showing very low activity towards kappa -casein. The BGPF1 proteinase completely hydrolysed only beta -casein. The BGRA43 proteinase completely hydrolysed all three casein fractions. The proteolytic activities of whole cells were inhibited by serine proteinase inhibitors, suggesting that all four strains produce serine proteinases. DNA-DNA hybridization and PCR analysis showed that BGPF1 contains the prtB-like proteinase gene. Characterized thermophilic strains BGPF1 and BGRA43 were successfully used as starter cultures for production of yoghurt and acidophilus milk, respectively.
PB  - Wiley
T2  - Journal of Applied Microbiology
T1  - Characterization of cell envelope-associated proteinases of thermophilic lactobacilli
EP  - 130
IS  - 1
SP  - 123
VL  - 90
DO  - 10.1046/j.1365-2672.2001.01226.x
ER  - 

@article{
author = "Fira, Đorđe and Kojić, Milan and Banina, Ana and Spasojević, I and Strahinić, Ivana and Topisirović, Ljubiša",
year = "2001",
abstract = "The proteolytic activities of two natural isolates of thermophilic lactobacilli, Lactobacillus acidophilus BGRA43 and Lact, delbrueckii BGPF1, and Lact. acidophilus CH2 (Chr. Hansen's strain) and Lact, acidophilus V74 (Visby's strain), were compared. Results revealed that optimal pH for all four proteinases is 6.5, whereas temperature optimum varied among proteinases. Determination of caseinolytic activity done under optimal conditions for each strain revealed that the CH2 and V74 proteinases completely hydrolysed both alpha (s1)-casein and beta -casein, showing very low activity towards kappa -casein. The BGPF1 proteinase completely hydrolysed only beta -casein. The BGRA43 proteinase completely hydrolysed all three casein fractions. The proteolytic activities of whole cells were inhibited by serine proteinase inhibitors, suggesting that all four strains produce serine proteinases. DNA-DNA hybridization and PCR analysis showed that BGPF1 contains the prtB-like proteinase gene. Characterized thermophilic strains BGPF1 and BGRA43 were successfully used as starter cultures for production of yoghurt and acidophilus milk, respectively.",
publisher = "Wiley",
journal = "Journal of Applied Microbiology",
title = "Characterization of cell envelope-associated proteinases of thermophilic lactobacilli",
pages = "130-123",
number = "1",
volume = "90",
doi = "10.1046/j.1365-2672.2001.01226.x"
}

Fira, Đ., Kojić, M., Banina, A., Spasojević, I., Strahinić, I.,& Topisirović, L.. (2001). Characterization of cell envelope-associated proteinases of thermophilic lactobacilli. in Journal of Applied Microbiology
Wiley., 90(1), 123-130.
https://doi.org/10.1046/j.1365-2672.2001.01226.x

Fira Đ, Kojić M, Banina A, Spasojević I, Strahinić I, Topisirović L. Characterization of cell envelope-associated proteinases of thermophilic lactobacilli. in Journal of Applied Microbiology. 2001;90(1):123-130.
doi:10.1046/j.1365-2672.2001.01226.x .

Fira, Đorđe, Kojić, Milan, Banina, Ana, Spasojević, I, Strahinić, Ivana, Topisirović, Ljubiša, "Characterization of cell envelope-associated proteinases of thermophilic lactobacilli" in Journal of Applied Microbiology, 90, no. 1 (2001):123-130,
https://doi.org/10.1046/j.1365-2672.2001.01226.x . .

TY  - JOUR
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Miladinov, Nataša
AU  - Strahinić, Ivana
AU  - Gajić, Olivera
AU  - Arsenijević, Slavica
AU  - Spasojević, Irena
AU  - Vukasinović, Maja
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/793
AB  - Autohtoni sojevi bakterija mlečne kiseline (BMK) su izolovane iz fermentisanih mlečnih proizvoda rađenih u domaćoj radinosti. Fermentisani mlečni proizvodi su sakupljani sa specifičnih ekoloških lokaliteta kao što su visoke planine (iznad 1200 m nadmorske visine), planinske visoravni, rečne doline, ostrva, obala Jadranskog mora, itd. Analiza kolekcije prirodnih izolata BMK je pokazala da izolati proizvode proteinaze, bakteriocine i egzopolisaharide. Pokazano je, takođe, da neki izolati laktokoka i laktobacila proizvode istovremeno dva različita bakteriocina. Pored toga određeni broj izolata proizvodi i proteinaze i bakteriocine. Nađeno je da prirodni izolati sintetišu specifične proteinaze. Analiza organizacije prt gena u prirodnim izolatima BMK je pokazala da četiri laktobacila poseduju organizaciju prt gena koja se razlikuje od do sada opisanih. Izučavanje regulacije gena koji kodiraju proteinaze, bakteriocine ili egzopolisaharide može olakšati konstrukciju specifičnih starter kultura za proizvodnju autohtonih fermentisanih mlečnih proizvoda, tj. fermentisanih proizvoda sa geografskim poreklom.
AB  - Autochthonous strains of lactic acid bacteria (LAB) were isolated from homemade fermented milk products. These products were collected from specific ecological localities such as high mountains (above 1200 m above sea level) mountain plateaus, river valleys, islands, Adriatic coast, etc. Analysis of LAB from the collection of natural isolates revealed that they produce proteinases, bacteriocins and exopolysaccharides. It was also shown that some isolates of lactococci and lactobacilli produce two bacteriocins simultaneously. According to their antimicrobial and biochemical properties most of the analyzed bacteriocins in natural isolates of lactococci were class 11 bacteriocins. In addition some isolates produced both proteinase and bacteriocin. Biosynthesis of specific proteinases was detected in natural isolates. Analysis of the prt gene organization among natural isolates of LAB showed that four isolates of lactobacilli exhibited prt genes organization different from those described so far. Elucidation of the regulation of the genes encoding proteinases, bacteriocins and exopolysaccharides could facilitate the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. fermented products with a geographical origin.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Molekularna genetika autohtonih bakterija mlečne kiseline
T1  - Molecular genetics of autochthonous lactic acid bacteria
EP  - 127
IS  - 2
SP  - 115
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_intor_793
ER  - 

@article{
author = "Topisirović, Ljubiša and Kojić, Milan and Fira, Đorđe and Miladinov, Nataša and Strahinić, Ivana and Gajić, Olivera and Arsenijević, Slavica and Spasojević, Irena and Vukasinović, Maja",
year = "2000",
abstract = "Autohtoni sojevi bakterija mlečne kiseline (BMK) su izolovane iz fermentisanih mlečnih proizvoda rađenih u domaćoj radinosti. Fermentisani mlečni proizvodi su sakupljani sa specifičnih ekoloških lokaliteta kao što su visoke planine (iznad 1200 m nadmorske visine), planinske visoravni, rečne doline, ostrva, obala Jadranskog mora, itd. Analiza kolekcije prirodnih izolata BMK je pokazala da izolati proizvode proteinaze, bakteriocine i egzopolisaharide. Pokazano je, takođe, da neki izolati laktokoka i laktobacila proizvode istovremeno dva različita bakteriocina. Pored toga određeni broj izolata proizvodi i proteinaze i bakteriocine. Nađeno je da prirodni izolati sintetišu specifične proteinaze. Analiza organizacije prt gena u prirodnim izolatima BMK je pokazala da četiri laktobacila poseduju organizaciju prt gena koja se razlikuje od do sada opisanih. Izučavanje regulacije gena koji kodiraju proteinaze, bakteriocine ili egzopolisaharide može olakšati konstrukciju specifičnih starter kultura za proizvodnju autohtonih fermentisanih mlečnih proizvoda, tj. fermentisanih proizvoda sa geografskim poreklom., Autochthonous strains of lactic acid bacteria (LAB) were isolated from homemade fermented milk products. These products were collected from specific ecological localities such as high mountains (above 1200 m above sea level) mountain plateaus, river valleys, islands, Adriatic coast, etc. Analysis of LAB from the collection of natural isolates revealed that they produce proteinases, bacteriocins and exopolysaccharides. It was also shown that some isolates of lactococci and lactobacilli produce two bacteriocins simultaneously. According to their antimicrobial and biochemical properties most of the analyzed bacteriocins in natural isolates of lactococci were class 11 bacteriocins. In addition some isolates produced both proteinase and bacteriocin. Biosynthesis of specific proteinases was detected in natural isolates. Analysis of the prt gene organization among natural isolates of LAB showed that four isolates of lactobacilli exhibited prt genes organization different from those described so far. Elucidation of the regulation of the genes encoding proteinases, bacteriocins and exopolysaccharides could facilitate the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. fermented products with a geographical origin.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Molekularna genetika autohtonih bakterija mlečne kiseline, Molecular genetics of autochthonous lactic acid bacteria",
pages = "127-115",
number = "2",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_intor_793"
}

Topisirović, L., Kojić, M., Fira, Đ., Miladinov, N., Strahinić, I., Gajić, O., Arsenijević, S., Spasojević, I.,& Vukasinović, M.. (2000). Molekularna genetika autohtonih bakterija mlečne kiseline. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 32(2), 115-127.
https://hdl.handle.net/21.15107/rcub_intor_793

Topisirović L, Kojić M, Fira Đ, Miladinov N, Strahinić I, Gajić O, Arsenijević S, Spasojević I, Vukasinović M. Molekularna genetika autohtonih bakterija mlečne kiseline. in Genetika-Belgrade. 2000;32(2):115-127.
https://hdl.handle.net/21.15107/rcub_intor_793 .

Topisirović, Ljubiša, Kojić, Milan, Fira, Đorđe, Miladinov, Nataša, Strahinić, Ivana, Gajić, Olivera, Arsenijević, Slavica, Spasojević, Irena, Vukasinović, Maja, "Molekularna genetika autohtonih bakterija mlečne kiseline" in Genetika-Belgrade, 32, no. 2 (2000):115-127,
https://hdl.handle.net/21.15107/rcub_intor_793 .

TY  - JOUR
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Arsenijević, Slavica
AU  - Banina, Ana
AU  - Topisirović, Ljubiša
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/792
AB  - Enterococcus faecalis BGPM3 proizvodi proteinazu sposobnu da hidrolizuje ukupan kazein kao i frakcije αs1-, β-, i k-kazeina. Ova proteinaza, takođe, hidrolizuje želatin, ali ne deluje na denaturisani goveđi serum albumin ili hemoglobin. Ustanovljeno je da se optimalna hidroliza kazeina u prisuetvu BGPM3 proteinaze postiže na pH 6.5, a njegova maksimalna hidroliza na 37°C. Prisustvo proteolitičke aktivnosti u supernatantu, koji ne sadrži žive ćelije, ukazuje da izolat E. faecalis BGPM3 proizvodi ekstracelularnu proteinazu. Sinteza ove proteinaze se odigrava tokom celokupnog ciklusa rasta bakterije, pri čemu se maksimum proizvodnje postiže u stacionarnoj fazi. Trstman BGPM3 proteinaze sa helatorima metalnih jona dovodi do potpunog gubitka proteolitičke aktivnosti. Međutim, moguće je povratiti proteolitičku aktivnost (do 75%) ako se tretiranom enzimu dodaju joni Zn2+ što ukazuje da je BGPM3 proteinaza metaloenzim. Proteolitička aktivnost ovog enzima je inhibirana jonima Cu2+, čak i u prisustvu jona Zn2+. Eksperimentalni rezultati ukazuju da je proizvodnja BGPM3 proteinaze indudibilna, tj., dolazi do povećanja njene sinteze kada bakterija raste u prisustvu smeše oligopeptida (kazitona). Tako se dobija desetostruko povećanje proteolitičke aktivnosti u bezćelijskom supernatantu kada se pripremi iz kulture koja sadrži kaziton. Određivanje molekulske mase BGPM3 proteinaze je pokazalo da je to protein od oko 29 kDa.
AB  - Enterococcus faecalis BGPM3 produces a proteinase that hydrolyzes total casein as well as α s1-, β-, and k-casein fractions. This proteinase was also able to hydrolyse gelatine, but not denatured bovine serum albumin and haemoglobin. The optimal pH of casein hydrolysis was 6.5 (determined at 30°C). Maximum caseinolytic activity was obtained at 37°C. The presence of proteolytic activity in cell-free supernatant strongly indicated that E. faecalis BGPM3 produces strictly extracellular proteinase. Proteinase production occurred through the growth cycle reaching a maximum at stationary phase. Pretreatment of the BGPM3 proteinase with metal ion chelators resulted in a total loss of proteolytic activity. Restoration of activity (75%) was obtained only with Zn2+ suggesting that the BGPM3 proteinase is zinc-metalloenzyme. Cu2+ even in the presence of Zn2+ inhibited proteolytic activity. It seems that production of proteinase is induced by oligopeptides (casitone), since 10-fold higher proteolytic activity was obtained in the cell-free supernatant prepared from the cultures containing casitone. The molecular mass determination revealed that extracellular BGPM3 proteinase has a molecular mass of about 29 kDa.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Proizvodnja inducibilne ekstracelularne proteinaze pomoću prirodnog izolata Enterococcus faecalis BGPM3
T1  - Natural isolate Enterococcus faecalis BGPM3 produces an inducible extracellular proteinase
EP  - 76
IS  - 2
SP  - 67
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_intor_792
ER  - 

@article{
author = "Fira, Đorđe and Kojić, Milan and Strahinić, Ivana and Arsenijević, Slavica and Banina, Ana and Topisirović, Ljubiša",
year = "2000",
abstract = "Enterococcus faecalis BGPM3 proizvodi proteinazu sposobnu da hidrolizuje ukupan kazein kao i frakcije αs1-, β-, i k-kazeina. Ova proteinaza, takođe, hidrolizuje želatin, ali ne deluje na denaturisani goveđi serum albumin ili hemoglobin. Ustanovljeno je da se optimalna hidroliza kazeina u prisuetvu BGPM3 proteinaze postiže na pH 6.5, a njegova maksimalna hidroliza na 37°C. Prisustvo proteolitičke aktivnosti u supernatantu, koji ne sadrži žive ćelije, ukazuje da izolat E. faecalis BGPM3 proizvodi ekstracelularnu proteinazu. Sinteza ove proteinaze se odigrava tokom celokupnog ciklusa rasta bakterije, pri čemu se maksimum proizvodnje postiže u stacionarnoj fazi. Trstman BGPM3 proteinaze sa helatorima metalnih jona dovodi do potpunog gubitka proteolitičke aktivnosti. Međutim, moguće je povratiti proteolitičku aktivnost (do 75%) ako se tretiranom enzimu dodaju joni Zn2+ što ukazuje da je BGPM3 proteinaza metaloenzim. Proteolitička aktivnost ovog enzima je inhibirana jonima Cu2+, čak i u prisustvu jona Zn2+. Eksperimentalni rezultati ukazuju da je proizvodnja BGPM3 proteinaze indudibilna, tj., dolazi do povećanja njene sinteze kada bakterija raste u prisustvu smeše oligopeptida (kazitona). Tako se dobija desetostruko povećanje proteolitičke aktivnosti u bezćelijskom supernatantu kada se pripremi iz kulture koja sadrži kaziton. Određivanje molekulske mase BGPM3 proteinaze je pokazalo da je to protein od oko 29 kDa., Enterococcus faecalis BGPM3 produces a proteinase that hydrolyzes total casein as well as α s1-, β-, and k-casein fractions. This proteinase was also able to hydrolyse gelatine, but not denatured bovine serum albumin and haemoglobin. The optimal pH of casein hydrolysis was 6.5 (determined at 30°C). Maximum caseinolytic activity was obtained at 37°C. The presence of proteolytic activity in cell-free supernatant strongly indicated that E. faecalis BGPM3 produces strictly extracellular proteinase. Proteinase production occurred through the growth cycle reaching a maximum at stationary phase. Pretreatment of the BGPM3 proteinase with metal ion chelators resulted in a total loss of proteolytic activity. Restoration of activity (75%) was obtained only with Zn2+ suggesting that the BGPM3 proteinase is zinc-metalloenzyme. Cu2+ even in the presence of Zn2+ inhibited proteolytic activity. It seems that production of proteinase is induced by oligopeptides (casitone), since 10-fold higher proteolytic activity was obtained in the cell-free supernatant prepared from the cultures containing casitone. The molecular mass determination revealed that extracellular BGPM3 proteinase has a molecular mass of about 29 kDa.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Proizvodnja inducibilne ekstracelularne proteinaze pomoću prirodnog izolata Enterococcus faecalis BGPM3, Natural isolate Enterococcus faecalis BGPM3 produces an inducible extracellular proteinase",
pages = "76-67",
number = "2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_intor_792"
}

Fira, Đ., Kojić, M., Strahinić, I., Arsenijević, S., Banina, A.,& Topisirović, L.. (2000). Proizvodnja inducibilne ekstracelularne proteinaze pomoću prirodnog izolata Enterococcus faecalis BGPM3. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 52(2), 67-76.
https://hdl.handle.net/21.15107/rcub_intor_792

Fira Đ, Kojić M, Strahinić I, Arsenijević S, Banina A, Topisirović L. Proizvodnja inducibilne ekstracelularne proteinaze pomoću prirodnog izolata Enterococcus faecalis BGPM3. in Archives of Biological Sciences. 2000;52(2):67-76.
https://hdl.handle.net/21.15107/rcub_intor_792 .

Fira, Đorđe, Kojić, Milan, Strahinić, Ivana, Arsenijević, Slavica, Banina, Ana, Topisirović, Ljubiša, "Proizvodnja inducibilne ekstracelularne proteinaze pomoću prirodnog izolata Enterococcus faecalis BGPM3" in Archives of Biological Sciences, 52, no. 2 (2000):67-76,
https://hdl.handle.net/21.15107/rcub_intor_792 .

TY  - JOUR
AU  - Banina, Ana
AU  - Vukasinović, M
AU  - Branković, S
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 1998
UR  - http://intor.torlakinstitut.com/handle/123456789/718
AB  - Lactobacillus acidophilus BGRA43 was selected from a set of human origin isolates of Lact. acidophilus strains for the highest growth rates and antagonistic effect against both Gram-positive and Gram-negative bacteria. The strain BGRA43 also exhibited an inhibitory effect on the growth of Clostridium sporogenes. Inhibition of this strain seems to be due to lactic acid production rather than hydrogen peroxide or bacteriocin. Growth of Lact. acidophilus BGRA43 in non-fat skim milk for 6 h at 37 degrees C resulted in a lowering of the pH value to 4.53. Besides the fast acidification, this strain generated a high viscosity of skim milk. These characteristics make the strain BGRA43 attractive for acidophilus milk production. Lactobacillus acidophilus BGRA43 produces an extracellular proteinase. Whole cells efficiently degraded casein for 3 h at 37 degrees C especially alpha- and beta-casein fractions. Total DNA isolated from the strain BGRA43 did not show any hybridization with lactococcal proteinase probes indicating that this strain produces a distinctive proteinase.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production
EP  - 599
IS  - 4
SP  - 593
VL  - 84
DO  - 10.1046/j.1365-2672.1998.00386.x
ER  - 

@article{
author = "Banina, Ana and Vukasinović, M and Branković, S and Fira, Đorđe and Kojić, Milan and Topisirović, Ljubiša",
year = "1998",
abstract = "Lactobacillus acidophilus BGRA43 was selected from a set of human origin isolates of Lact. acidophilus strains for the highest growth rates and antagonistic effect against both Gram-positive and Gram-negative bacteria. The strain BGRA43 also exhibited an inhibitory effect on the growth of Clostridium sporogenes. Inhibition of this strain seems to be due to lactic acid production rather than hydrogen peroxide or bacteriocin. Growth of Lact. acidophilus BGRA43 in non-fat skim milk for 6 h at 37 degrees C resulted in a lowering of the pH value to 4.53. Besides the fast acidification, this strain generated a high viscosity of skim milk. These characteristics make the strain BGRA43 attractive for acidophilus milk production. Lactobacillus acidophilus BGRA43 produces an extracellular proteinase. Whole cells efficiently degraded casein for 3 h at 37 degrees C especially alpha- and beta-casein fractions. Total DNA isolated from the strain BGRA43 did not show any hybridization with lactococcal proteinase probes indicating that this strain produces a distinctive proteinase.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production",
pages = "599-593",
number = "4",
volume = "84",
doi = "10.1046/j.1365-2672.1998.00386.x"
}

Banina, A., Vukasinović, M., Branković, S., Fira, Đ., Kojić, M.,& Topisirović, L.. (1998). Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production. in Journal of Applied Microbiology
Wiley, Hoboken., 84(4), 593-599.
https://doi.org/10.1046/j.1365-2672.1998.00386.x

Banina A, Vukasinović M, Branković S, Fira Đ, Kojić M, Topisirović L. Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production. in Journal of Applied Microbiology. 1998;84(4):593-599.
doi:10.1046/j.1365-2672.1998.00386.x .

Banina, Ana, Vukasinović, M, Branković, S, Fira, Đorđe, Kojić, Milan, Topisirović, Ljubiša, "Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production" in Journal of Applied Microbiology, 84, no. 4 (1998):593-599,
https://doi.org/10.1046/j.1365-2672.1998.00386.x . .