Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation
Само за регистроване кориснике
2006
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Autochthonous strains of lactic acid bacteria (LAB) have been isolated from traditionally homemade cheeses collected from specific ecological localities across Serbia and Montenegro. Genetic and biochemical analysis of this LAB revealed that they produce bacteriocins, proteinases and exopolysaccharides. LAB produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. Apart from the metabolic end products, some strains also secrete antimicrobial substances known as bacteriocins. Among the natural isolates of LAB from homemade cheeses, bacteriocin producers were found in both lactococci and lactobacilli. Lactococcus lactis subsp. lactis BGMN1-5 was found to produce three narrow spectrum class II heat-stable bacteriocins. In addition to bacteriocin production, BGMN1-5 synthesized a cell envelope-associated protemase (CEP) and shows an aggregation phenotype. Another isolate, L. lactis subsp. lactis BGSM1-19 produces low molecular mass (7 ...kDa) bacteriocin SM19 that showed antimicrobial activity against Staphylococcus aureus, Micrococcus,flavus and partially against Salmonella paratyphi. Production of bacteriocin reaches a plateau after 8 h of BGSM1-19 growth. Bacteriocin SM19 retained activity within the wide pH range from I to 12 and after the treatment at 100 degrees C for 15 min. Among collection of lactobacilli, the isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces heat-stable bacteriocin SJ (approx. 5 kDa) polypeptide. It retained activity after treatment for 1 h at 100 degrees C, and in the pH range from 2 to 11. In addition to isolates from cheeses, bacteriocin-producing human oral lactobacilli were detected. Most of them showed antimicrobial activity against streptococci, staphylococci and micrococci, but not against Candida. Isolate BGHO1 that showed the highest antimicrobial activity was determined as L. paracasei. Interestingly, Lactobacillus helveticus BGRA43, which was isolated from the human intestine showed strong activity against Clostridium sporogenes, but it was not possible to detect any bacteriocin production in this isolate by using standard procedures. Further analysis of antimicrobial activity revealed that BGRA43 has a relatively broad spectrum. Lactobacilli resistant to nisin were also detected among natural isolates. They produce bacteriocins, which have no activity against nisin producing lactococci.
Кључне речи:
resistance to nisin / LAB natural isolates / exopolysaccharides / bacteriocinsИзвор:
International Journal of Food Microbiology, 2006, 112, 3, 230-235Издавач:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/j.ijfoodmicro.2006.04.009
ISSN: 0168-1605
PubMed: 16764959
WoS: 000242639400007
Scopus: 2-s2.0-33750721735
Институција/група
TorlakTY - CONF AU - Topisirović, Ljubiša AU - Kojić, Milan AU - Fira, Đorđe AU - Golić, Nataša AU - Strahinić, Ivana AU - Lozo, Jelena PY - 2006 UR - http://intor.torlakinstitut.com/handle/123456789/811 AB - Autochthonous strains of lactic acid bacteria (LAB) have been isolated from traditionally homemade cheeses collected from specific ecological localities across Serbia and Montenegro. Genetic and biochemical analysis of this LAB revealed that they produce bacteriocins, proteinases and exopolysaccharides. LAB produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. Apart from the metabolic end products, some strains also secrete antimicrobial substances known as bacteriocins. Among the natural isolates of LAB from homemade cheeses, bacteriocin producers were found in both lactococci and lactobacilli. Lactococcus lactis subsp. lactis BGMN1-5 was found to produce three narrow spectrum class II heat-stable bacteriocins. In addition to bacteriocin production, BGMN1-5 synthesized a cell envelope-associated protemase (CEP) and shows an aggregation phenotype. Another isolate, L. lactis subsp. lactis BGSM1-19 produces low molecular mass (7 kDa) bacteriocin SM19 that showed antimicrobial activity against Staphylococcus aureus, Micrococcus,flavus and partially against Salmonella paratyphi. Production of bacteriocin reaches a plateau after 8 h of BGSM1-19 growth. Bacteriocin SM19 retained activity within the wide pH range from I to 12 and after the treatment at 100 degrees C for 15 min. Among collection of lactobacilli, the isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces heat-stable bacteriocin SJ (approx. 5 kDa) polypeptide. It retained activity after treatment for 1 h at 100 degrees C, and in the pH range from 2 to 11. In addition to isolates from cheeses, bacteriocin-producing human oral lactobacilli were detected. Most of them showed antimicrobial activity against streptococci, staphylococci and micrococci, but not against Candida. Isolate BGHO1 that showed the highest antimicrobial activity was determined as L. paracasei. Interestingly, Lactobacillus helveticus BGRA43, which was isolated from the human intestine showed strong activity against Clostridium sporogenes, but it was not possible to detect any bacteriocin production in this isolate by using standard procedures. Further analysis of antimicrobial activity revealed that BGRA43 has a relatively broad spectrum. Lactobacilli resistant to nisin were also detected among natural isolates. They produce bacteriocins, which have no activity against nisin producing lactococci. PB - Elsevier Science Bv, Amsterdam C3 - International Journal of Food Microbiology T1 - Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation EP - 235 IS - 3 SP - 230 VL - 112 DO - 10.1016/j.ijfoodmicro.2006.04.009 ER -
@conference{ author = "Topisirović, Ljubiša and Kojić, Milan and Fira, Đorđe and Golić, Nataša and Strahinić, Ivana and Lozo, Jelena", year = "2006", abstract = "Autochthonous strains of lactic acid bacteria (LAB) have been isolated from traditionally homemade cheeses collected from specific ecological localities across Serbia and Montenegro. Genetic and biochemical analysis of this LAB revealed that they produce bacteriocins, proteinases and exopolysaccharides. LAB produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. Apart from the metabolic end products, some strains also secrete antimicrobial substances known as bacteriocins. Among the natural isolates of LAB from homemade cheeses, bacteriocin producers were found in both lactococci and lactobacilli. Lactococcus lactis subsp. lactis BGMN1-5 was found to produce three narrow spectrum class II heat-stable bacteriocins. In addition to bacteriocin production, BGMN1-5 synthesized a cell envelope-associated protemase (CEP) and shows an aggregation phenotype. Another isolate, L. lactis subsp. lactis BGSM1-19 produces low molecular mass (7 kDa) bacteriocin SM19 that showed antimicrobial activity against Staphylococcus aureus, Micrococcus,flavus and partially against Salmonella paratyphi. Production of bacteriocin reaches a plateau after 8 h of BGSM1-19 growth. Bacteriocin SM19 retained activity within the wide pH range from I to 12 and after the treatment at 100 degrees C for 15 min. Among collection of lactobacilli, the isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8 produces heat-stable bacteriocin SJ (approx. 5 kDa) polypeptide. It retained activity after treatment for 1 h at 100 degrees C, and in the pH range from 2 to 11. In addition to isolates from cheeses, bacteriocin-producing human oral lactobacilli were detected. Most of them showed antimicrobial activity against streptococci, staphylococci and micrococci, but not against Candida. Isolate BGHO1 that showed the highest antimicrobial activity was determined as L. paracasei. Interestingly, Lactobacillus helveticus BGRA43, which was isolated from the human intestine showed strong activity against Clostridium sporogenes, but it was not possible to detect any bacteriocin production in this isolate by using standard procedures. Further analysis of antimicrobial activity revealed that BGRA43 has a relatively broad spectrum. Lactobacilli resistant to nisin were also detected among natural isolates. They produce bacteriocins, which have no activity against nisin producing lactococci.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Journal of Food Microbiology", title = "Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation", pages = "235-230", number = "3", volume = "112", doi = "10.1016/j.ijfoodmicro.2006.04.009" }
Topisirović, L., Kojić, M., Fira, Đ., Golić, N., Strahinić, I.,& Lozo, J.. (2006). Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. in International Journal of Food Microbiology Elsevier Science Bv, Amsterdam., 112(3), 230-235. https://doi.org/10.1016/j.ijfoodmicro.2006.04.009
Topisirović L, Kojić M, Fira Đ, Golić N, Strahinić I, Lozo J. Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. in International Journal of Food Microbiology. 2006;112(3):230-235. doi:10.1016/j.ijfoodmicro.2006.04.009 .
Topisirović, Ljubiša, Kojić, Milan, Fira, Đorđe, Golić, Nataša, Strahinić, Ivana, Lozo, Jelena, "Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation" in International Journal of Food Microbiology, 112, no. 3 (2006):230-235, https://doi.org/10.1016/j.ijfoodmicro.2006.04.009 . .