Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria
Само за регистроване кориснике
2012
Аутори
Berić, TanjaKojić, Milan
Stanković, Slaviša
Topisirović, Ljubiša
Degrassi, Giuliano
Myers, Michael
Venturi, Vittorio
Fira, Đorđe
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with ...potential for biocontrol of X. oryzae pv. oryzae.
Кључне речи:
Xanthomonas oryzae pv. oryzae / lipopeptides / iturin / bacteriocin / Bacillus sp.Извор:
Food Technology and Biotechnology, 2012, 50, 1, 25-31Издавач:
- University of Zagreb
Финансирање / пројекти:
- Молекуларна карактеризација бактерија из родова Bacillus и Pseudomonas као потенцијалних агенаса за биолошку контролу (RS-MESTD-Basic Research (BR or ON)-173026)
- International Centre for Genetic Engineering and Biotechnology, Italy [CRP-YUG06/01]
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/574http://intor.torlakinstitut.com/handle/123456789/691
Институција/група
TorlakTY - JOUR AU - Berić, Tanja AU - Kojić, Milan AU - Stanković, Slaviša AU - Topisirović, Ljubiša AU - Degrassi, Giuliano AU - Myers, Michael AU - Venturi, Vittorio AU - Fira, Đorđe PY - 2012 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/574 UR - http://intor.torlakinstitut.com/handle/123456789/691 AB - Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae. PB - University of Zagreb T2 - Food Technology and Biotechnology T1 - Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria EP - 31 IS - 1 SP - 25 VL - 50 UR - https://hdl.handle.net/21.15107/rcub_imagine_574 ER -
@article{ author = "Berić, Tanja and Kojić, Milan and Stanković, Slaviša and Topisirović, Ljubiša and Degrassi, Giuliano and Myers, Michael and Venturi, Vittorio and Fira, Đorđe", year = "2012", abstract = "Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.", publisher = "University of Zagreb", journal = "Food Technology and Biotechnology", title = "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria", pages = "31-25", number = "1", volume = "50", url = "https://hdl.handle.net/21.15107/rcub_imagine_574" }
Berić, T., Kojić, M., Stanković, S., Topisirović, L., Degrassi, G., Myers, M., Venturi, V.,& Fira, Đ.. (2012). Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology University of Zagreb., 50(1), 25-31. https://hdl.handle.net/21.15107/rcub_imagine_574
Berić T, Kojić M, Stanković S, Topisirović L, Degrassi G, Myers M, Venturi V, Fira Đ. Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology. 2012;50(1):25-31. https://hdl.handle.net/21.15107/rcub_imagine_574 .
Berić, Tanja, Kojić, Milan, Stanković, Slaviša, Topisirović, Ljubiša, Degrassi, Giuliano, Myers, Michael, Venturi, Vittorio, Fira, Đorđe, "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria" in Food Technology and Biotechnology, 50, no. 1 (2012):25-31, https://hdl.handle.net/21.15107/rcub_imagine_574 .