Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain... L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.
Кључне речи:
replication / plasmid sequences / oriT / conjugationИзвор:
Journal of Applied Microbiology, 2009, 106, 1, 78-88Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Изучавање регулације експресије гена одабраних индустријских микроорганизама (RS-MESTD-MPN2006-2010-143036)
DOI: 10.1111/j.1365-2672.2008.03977.x
ISSN: 1364-5072
PubMed: 19040703
WoS: 000262475600009
Scopus: 2-s2.0-58249102177
Институција/група
TorlakTY - JOUR AU - Strahinić, Ivana AU - Kojić, Milan AU - Tolinački, Maja AU - Fira, Đorđe AU - Topisirović, Ljubiša PY - 2009 UR - http://intor.torlakinstitut.com/handle/123456789/821 AB - Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci. PB - Wiley, Hoboken T2 - Journal of Applied Microbiology T1 - Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors EP - 88 IS - 1 SP - 78 VL - 106 DO - 10.1111/j.1365-2672.2008.03977.x ER -
@article{ author = "Strahinić, Ivana and Kojić, Milan and Tolinački, Maja and Fira, Đorđe and Topisirović, Ljubiša", year = "2009", abstract = "Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.", publisher = "Wiley, Hoboken", journal = "Journal of Applied Microbiology", title = "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors", pages = "88-78", number = "1", volume = "106", doi = "10.1111/j.1365-2672.2008.03977.x" }
Strahinić, I., Kojić, M., Tolinački, M., Fira, Đ.,& Topisirović, L.. (2009). Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology Wiley, Hoboken., 106(1), 78-88. https://doi.org/10.1111/j.1365-2672.2008.03977.x
Strahinić I, Kojić M, Tolinački M, Fira Đ, Topisirović L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology. 2009;106(1):78-88. doi:10.1111/j.1365-2672.2008.03977.x .
Strahinić, Ivana, Kojić, Milan, Tolinački, Maja, Fira, Đorđe, Topisirović, Ljubiša, "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors" in Journal of Applied Microbiology, 106, no. 1 (2009):78-88, https://doi.org/10.1111/j.1365-2672.2008.03977.x . .