Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon
Authorized Users Only
2016
Authors
Marinković, EmilijaLukić, Ivana
Kosanović, Dejana
Inić-Kanada, Aleksandra
Gavrović-Jankulović, Marija
Stojanović, Marijana
Article (Published version)
Metadata
Show full item recordAbstract
Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. ...(C) 2015 Elsevier Ltd. All rights reserved.
Keywords:
Banana lectin / Colon / ImmunostimulationSource:
Journal of Functional Foods, 2016, 20, 68-78Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1016/j.jff.2015.10.019
ISSN: 1756-4646
WoS: 000375634900007
Scopus: 2-s2.0-84953386247
Collections
Institution/Community
TorlakTY - JOUR AU - Marinković, Emilija AU - Lukić, Ivana AU - Kosanović, Dejana AU - Inić-Kanada, Aleksandra AU - Gavrović-Jankulović, Marija AU - Stojanović, Marijana PY - 2016 UR - http://intor.torlakinstitut.com/handle/123456789/471 AB - Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved. PB - Elsevier, Amsterdam T2 - Journal of Functional Foods T1 - Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon EP - 78 SP - 68 VL - 20 DO - 10.1016/j.jff.2015.10.019 ER -
@article{ author = "Marinković, Emilija and Lukić, Ivana and Kosanović, Dejana and Inić-Kanada, Aleksandra and Gavrović-Jankulović, Marija and Stojanović, Marijana", year = "2016", abstract = "Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Journal of Functional Foods", title = "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon", pages = "78-68", volume = "20", doi = "10.1016/j.jff.2015.10.019" }
Marinković, E., Lukić, I., Kosanović, D., Inić-Kanada, A., Gavrović-Jankulović, M.,& Stojanović, M.. (2016). Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods Elsevier, Amsterdam., 20, 68-78. https://doi.org/10.1016/j.jff.2015.10.019
Marinković E, Lukić I, Kosanović D, Inić-Kanada A, Gavrović-Jankulović M, Stojanović M. Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods. 2016;20:68-78. doi:10.1016/j.jff.2015.10.019 .
Marinković, Emilija, Lukić, Ivana, Kosanović, Dejana, Inić-Kanada, Aleksandra, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon" in Journal of Functional Foods, 20 (2016):68-78, https://doi.org/10.1016/j.jff.2015.10.019 . .