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Immunoglobulins G from patients with sporadic amyotrophic lateral sclerosis affects cytosolic Ca2+ homeostasis in cultured rat astrocytes
dc.creator | Milošević, Milena | |
dc.creator | Stenovec, Matjaz | |
dc.creator | Kreft, Marko | |
dc.creator | Petrušić, Vladimir | |
dc.creator | Stević, Zorica | |
dc.creator | Trkov, Saša | |
dc.creator | Anđus, Pavle | |
dc.creator | Zorec, Robert | |
dc.date.accessioned | 2021-02-18T10:40:47Z | |
dc.date.available | 2021-02-18T10:40:47Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0143-4160 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/378 | |
dc.description.abstract | Astrocytes are considered essential in the etiopathogenesis of amyotrophic lateral sclerosis (ALS). We have demonstrated previously that immunoglobulins G (IgG) isolated from patients with ALS enhance the mobility of acidic vesicles in cultured astrocytes in a Ca2+-dependent manner. Here we directly examined the impact of purified sporadic ALS IgG on cytosolic [Ca2+] ([Ca2+](i)) in astrocytes. Confocal time-lapse images were acquired and fluorescence of a non-ratiometric Ca2+ indicator was recorded before and after the application of IgG. ALS IgG (0.1 mg/ml) from 7 patients evoked transient increases in [Ca2+](i) in similar to 50% of tested astrocytes. The probability of observing a response was independent of extracellular Ca2+. The peak increase in [Ca2+](i) developed similar to 3 times faster and the time integral of evoked transients was similar to 2-fold larger; the peak amplitude itself was not affected by extracellular Ca2+. Application of pharmacological inhibitors revealed that activation of inositol-1,4,5-triphosphate receptors is necessary and sufficient to initiate transients in [Ca2+](i) the Ca2+ influx through store-operated calcium entry prolongs the transient increase in [Ca2+](i). Thus, ALS IgG acutely affect [Ca2+](i) by mobilizing both, intra- and extracellular Ca2+ into the cytosol of cultured astrocytes. (C) 2013 Elsevier Ltd. All rights reserved. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | Slovenian Research Agency (ARRS)Slovenian Research Agency - Slovenia [P3 310 0381] | |
dc.relation | EduGlia ESR grant | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41005/RS// | |
dc.rights | restrictedAccess | |
dc.source | Cell Calcium | |
dc.subject | Amyotrophic lateral sclerosis | en |
dc.subject | Astrocytes | en |
dc.subject | IgG | en |
dc.subject | Calcium homeostasis | en |
dc.subject | IP3 receptors | en |
dc.title | Immunoglobulins G from patients with sporadic amyotrophic lateral sclerosis affects cytosolic Ca2+ homeostasis in cultured rat astrocytes | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 25 | |
dc.citation.issue | 1 | |
dc.citation.other | 54(1): 17-25 | |
dc.citation.rank | M22 | |
dc.citation.spage | 17 | |
dc.citation.volume | 54 | |
dc.identifier.doi | 10.1016/j.ceca.2013.03.005 | |
dc.identifier.pmid | 23623373 | |
dc.identifier.scopus | 2-s2.0-84878218847 | |
dc.identifier.wos | 000320681000002 | |
dc.type.version | publishedVersion |