Stability evaluation of house dust mite vaccines for sublingual immunotherapy
Ispitivanje stabilnosti vakcine kućne prašine za sublingvalnu imunoterapiju
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Allergen-specific immunotherapy with house dust mite (HDM) allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt) mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 °C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65-80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4°C preserved their IgE-binding potential during a 12 month period, implying their s...uitability for sublingual immunotherapeutic treatment of HDM allergy.
Alergen-specifična imunoterapija predstavlja postupak koji može da promeni tok bolesti kod alergijskog rinitisa i astme. Kvalitet vakcine pripremljene od kućnih grinja značajno utiče na efikasnost imunoterapeutskog tretmana. Ispitivanje je imalo za cilj procenu stabilnosti vakcine kućnih grinja namenjene za sublingvalnu imunoterapiju. Telo liofilozovanih Dermatophagoides pteronyssinus grinja (Dpt-grinja) upotrebljeno je kao polazni materijal za proizvodnju sublingvalne vakcine u 4 različita terapeutska razblaženja. Potentnost Dpt vakcina praćena ELISA-inhibicijom u 4 terapeutska razblaženja, nakon 12 meseci zadržan je u svim razblaženjima u opsegu 65-80%. Ispitivanje je pokazalo da glicerolom stabilizovane Dpt vakcine za subligvalnu imunoterapiju, uz čuvanje na 4°C zadržavaju alergeni potencijal ( gt 65%) nakon 12 meseci od njihove pripreme, i kao takve mogu se koristiti za tretman alergije na kućne grinje.
Кључне речи:
Dermatophagoides pteronyssinus / allergen extract / vaccines / ELISA inhibition / stabilityИзвор:
Journal of the Serbian Chemical Society, 2010, 75, 1, 19-26Издавач:
- Srpsko hemijsko društvo, Beograd
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-MESTD-MPN2006-2010-142020)
DOI: 10.2298/JSC1001019B
ISSN: 0352-5139
WoS: 000274586300003
Scopus: 2-s2.0-77950951080
Институција/група
TorlakTY - JOUR AU - Burazer, Lidija AU - Milovanović, Katarina AU - Ćirković-Veličković, Tanja AU - Gavrović-Jankulović, Marija PY - 2010 UR - http://intor.torlakinstitut.com/handle/123456789/314 AB - Allergen-specific immunotherapy with house dust mite (HDM) allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt) mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 °C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65-80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4°C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy. AB - Alergen-specifična imunoterapija predstavlja postupak koji može da promeni tok bolesti kod alergijskog rinitisa i astme. Kvalitet vakcine pripremljene od kućnih grinja značajno utiče na efikasnost imunoterapeutskog tretmana. Ispitivanje je imalo za cilj procenu stabilnosti vakcine kućnih grinja namenjene za sublingvalnu imunoterapiju. Telo liofilozovanih Dermatophagoides pteronyssinus grinja (Dpt-grinja) upotrebljeno je kao polazni materijal za proizvodnju sublingvalne vakcine u 4 različita terapeutska razblaženja. Potentnost Dpt vakcina praćena ELISA-inhibicijom u 4 terapeutska razblaženja, nakon 12 meseci zadržan je u svim razblaženjima u opsegu 65-80%. Ispitivanje je pokazalo da glicerolom stabilizovane Dpt vakcine za subligvalnu imunoterapiju, uz čuvanje na 4°C zadržavaju alergeni potencijal ( gt 65%) nakon 12 meseci od njihove pripreme, i kao takve mogu se koristiti za tretman alergije na kućne grinje. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - Stability evaluation of house dust mite vaccines for sublingual immunotherapy T1 - Ispitivanje stabilnosti vakcine kućne prašine za sublingvalnu imunoterapiju EP - 26 IS - 1 SP - 19 VL - 75 DO - 10.2298/JSC1001019B ER -
@article{ author = "Burazer, Lidija and Milovanović, Katarina and Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija", year = "2010", abstract = "Allergen-specific immunotherapy with house dust mite (HDM) allergen extracts can effectively alleviate the symptoms of allergic rhinitis and asthma. The efficacy of the immunotherapeutic treatment is highly dependent on the quality of house dust mite vaccines. This study was performed to assess the stability of house dust mite allergen vaccines prepared for sublingual immunotherapy. Lyophilized Dermatophagoides pteronyssinus (Dpt) mite bodies were the starting material for the production of sublingual vaccines in four therapeutic concentrations. The stability of the extract for vaccine production, which was stored below 4 °C for one month, showed consistence in the protein profile in SDS PAGE. ELISA-inhibition showed that the potencies of Dpt vaccines during a 12 month period were to 65-80 % preserved at all analyzed therapeutic concentrations. This study showed that glycerinated Dpt vaccines stored at 4°C preserved their IgE-binding potential during a 12 month period, implying their suitability for sublingual immunotherapeutic treatment of HDM allergy., Alergen-specifična imunoterapija predstavlja postupak koji može da promeni tok bolesti kod alergijskog rinitisa i astme. Kvalitet vakcine pripremljene od kućnih grinja značajno utiče na efikasnost imunoterapeutskog tretmana. Ispitivanje je imalo za cilj procenu stabilnosti vakcine kućnih grinja namenjene za sublingvalnu imunoterapiju. Telo liofilozovanih Dermatophagoides pteronyssinus grinja (Dpt-grinja) upotrebljeno je kao polazni materijal za proizvodnju sublingvalne vakcine u 4 različita terapeutska razblaženja. Potentnost Dpt vakcina praćena ELISA-inhibicijom u 4 terapeutska razblaženja, nakon 12 meseci zadržan je u svim razblaženjima u opsegu 65-80%. Ispitivanje je pokazalo da glicerolom stabilizovane Dpt vakcine za subligvalnu imunoterapiju, uz čuvanje na 4°C zadržavaju alergeni potencijal ( gt 65%) nakon 12 meseci od njihove pripreme, i kao takve mogu se koristiti za tretman alergije na kućne grinje.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "Stability evaluation of house dust mite vaccines for sublingual immunotherapy, Ispitivanje stabilnosti vakcine kućne prašine za sublingvalnu imunoterapiju", pages = "26-19", number = "1", volume = "75", doi = "10.2298/JSC1001019B" }
Burazer, L., Milovanović, K., Ćirković-Veličković, T.,& Gavrović-Jankulović, M.. (2010). Stability evaluation of house dust mite vaccines for sublingual immunotherapy. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 75(1), 19-26. https://doi.org/10.2298/JSC1001019B
Burazer L, Milovanović K, Ćirković-Veličković T, Gavrović-Jankulović M. Stability evaluation of house dust mite vaccines for sublingual immunotherapy. in Journal of the Serbian Chemical Society. 2010;75(1):19-26. doi:10.2298/JSC1001019B .
Burazer, Lidija, Milovanović, Katarina, Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, "Stability evaluation of house dust mite vaccines for sublingual immunotherapy" in Journal of the Serbian Chemical Society, 75, no. 1 (2010):19-26, https://doi.org/10.2298/JSC1001019B . .