Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production
Abstract
PROBLEM: In addition to having an indirect effect on the T-cell development by controlling the production of ovarian steroids, an accumulating body of evidence suggest that GnRH analogue (GnRH-A) administration may exert a thymopoietic regulatory effect that is not mediated by ovarian hormones. METHOD OF STUDY: In non-ovariectomized (non-OVX) and OVX adult female AO rats treated s.c. with GnRH-A or saline (controls), over 14 days, were estimated the thymic cellularity and thymocyte expression of CD4/CD8/TCRalphabeta by stereological analysis and three-color flow cytometry, respectively. RESULTS: GnRH-A in both groups of rats diminished the thymic cellularity. In non-OVX rats GnRH-A increased the relative numbers of immature cells (CD4-8-TCRalphabeta(-), CD4-8-TCRalphabeta(low) and CD4+8-TCRalphabeta(low)), and reduced those of positively selected CD4+8+TCRalphabeta(high) and mature (CD4-8+TCRalphabeta(high), CD4+8-TCRalphabeta(high)) cells, suggesting decelerated expression of TCRalpha...beta followed by less efficient positive selection and further maturation of the selected cells. Differently, in OVX rats GnRH-A decreased the percentage of immature (CD4-8-TCRalphabeta(-), CD4+8+ TCRalphabeta(-)) cells and increased those of all TCRalphabeta(high) subsets, suggesting an increased rate of early thymocyte differentiation, more efficient positive selection and further maturation of the selected cells. CONCLUSIONS: The effect of GnRH-A administration is affected by the presence of ovarian steroids.
Keywords:
distribution of thymocyte subsets / GnRH agonist chronic treatment / thymic cellularitySource:
American Journal of Reproductive Immunology, 2005, 53, 1, 30-41Publisher:
- Wiley, Hoboken
DOI: 10.1111/j.1600-0897.2004.00244.x
ISSN: 1046-7408
PubMed: 15667523
WoS: 000226513500004
Scopus: 2-s2.0-13844266205
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TorlakTY - JOUR AU - Leposavić, Gordana AU - Pekić, S. AU - Kosec, Duško PY - 2005 UR - http://intor.torlakinstitut.com/handle/123456789/192 AB - PROBLEM: In addition to having an indirect effect on the T-cell development by controlling the production of ovarian steroids, an accumulating body of evidence suggest that GnRH analogue (GnRH-A) administration may exert a thymopoietic regulatory effect that is not mediated by ovarian hormones. METHOD OF STUDY: In non-ovariectomized (non-OVX) and OVX adult female AO rats treated s.c. with GnRH-A or saline (controls), over 14 days, were estimated the thymic cellularity and thymocyte expression of CD4/CD8/TCRalphabeta by stereological analysis and three-color flow cytometry, respectively. RESULTS: GnRH-A in both groups of rats diminished the thymic cellularity. In non-OVX rats GnRH-A increased the relative numbers of immature cells (CD4-8-TCRalphabeta(-), CD4-8-TCRalphabeta(low) and CD4+8-TCRalphabeta(low)), and reduced those of positively selected CD4+8+TCRalphabeta(high) and mature (CD4-8+TCRalphabeta(high), CD4+8-TCRalphabeta(high)) cells, suggesting decelerated expression of TCRalphabeta followed by less efficient positive selection and further maturation of the selected cells. Differently, in OVX rats GnRH-A decreased the percentage of immature (CD4-8-TCRalphabeta(-), CD4+8+ TCRalphabeta(-)) cells and increased those of all TCRalphabeta(high) subsets, suggesting an increased rate of early thymocyte differentiation, more efficient positive selection and further maturation of the selected cells. CONCLUSIONS: The effect of GnRH-A administration is affected by the presence of ovarian steroids. PB - Wiley, Hoboken T2 - American Journal of Reproductive Immunology T1 - Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production EP - 41 IS - 1 SP - 30 VL - 53 DO - 10.1111/j.1600-0897.2004.00244.x ER -
@article{ author = "Leposavić, Gordana and Pekić, S. and Kosec, Duško", year = "2005", abstract = "PROBLEM: In addition to having an indirect effect on the T-cell development by controlling the production of ovarian steroids, an accumulating body of evidence suggest that GnRH analogue (GnRH-A) administration may exert a thymopoietic regulatory effect that is not mediated by ovarian hormones. METHOD OF STUDY: In non-ovariectomized (non-OVX) and OVX adult female AO rats treated s.c. with GnRH-A or saline (controls), over 14 days, were estimated the thymic cellularity and thymocyte expression of CD4/CD8/TCRalphabeta by stereological analysis and three-color flow cytometry, respectively. RESULTS: GnRH-A in both groups of rats diminished the thymic cellularity. In non-OVX rats GnRH-A increased the relative numbers of immature cells (CD4-8-TCRalphabeta(-), CD4-8-TCRalphabeta(low) and CD4+8-TCRalphabeta(low)), and reduced those of positively selected CD4+8+TCRalphabeta(high) and mature (CD4-8+TCRalphabeta(high), CD4+8-TCRalphabeta(high)) cells, suggesting decelerated expression of TCRalphabeta followed by less efficient positive selection and further maturation of the selected cells. Differently, in OVX rats GnRH-A decreased the percentage of immature (CD4-8-TCRalphabeta(-), CD4+8+ TCRalphabeta(-)) cells and increased those of all TCRalphabeta(high) subsets, suggesting an increased rate of early thymocyte differentiation, more efficient positive selection and further maturation of the selected cells. CONCLUSIONS: The effect of GnRH-A administration is affected by the presence of ovarian steroids.", publisher = "Wiley, Hoboken", journal = "American Journal of Reproductive Immunology", title = "Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production", pages = "41-30", number = "1", volume = "53", doi = "10.1111/j.1600-0897.2004.00244.x" }
Leposavić, G., Pekić, S.,& Kosec, D.. (2005). Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production. in American Journal of Reproductive Immunology Wiley, Hoboken., 53(1), 30-41. https://doi.org/10.1111/j.1600-0897.2004.00244.x
Leposavić G, Pekić S, Kosec D. Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production. in American Journal of Reproductive Immunology. 2005;53(1):30-41. doi:10.1111/j.1600-0897.2004.00244.x .
Leposavić, Gordana, Pekić, S., Kosec, Duško, "Gonadotropin-releasing hormone agonist administration affects the thymopoiesis in adult female rats independently on gonadal hormone production" in American Journal of Reproductive Immunology, 53, no. 1 (2005):30-41, https://doi.org/10.1111/j.1600-0897.2004.00244.x . .