TY  - JOUR
AU  - Lukić, Ivana
AU  - Blagojević, Veljko
AU  - Minić, Rajna
AU  - Ivanović, Saša
AU  - Borozan, Sunčica
AU  - Ćupić, Vitomir
AU  - Živković, Irena
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/976
AB  - that can most closely evaluate the biological activity of the drug of interest. For testing the potency
of antivenom, these are the methods used to assess cytotoxicity. The aim of this study was to evaluate
the most commonly used cytotoxicity methods for determining the protective potency of the antivenom
Viekvin, which neutralizes Vipera ammodytes venom.
Material and methods: The selected methods are based on different biological mechanisms: MTT
assay, based on the activity of cell oxidoreductase enzymes; crystal violet staining, based on the degree
of cell adhesion; trypan blue staining, based on cell membrane permeability, and propidium iodide
staining, based on measurement of nucleic acids of dead cells. The pro-apoptotic effect of the venom
was also determined with annexin V staining.
Results: The IC50 value of V. ammodytes venom obtained by these methods was very similar, while
the EC50 values differed significantly.
Conclusions: We concluded that the choice of the method used to measure the anticytotoxic antivenom
potency depends on the immunogenicity of the venom components that cause cell death; for each
venom/antivenom pair, it is necessary to select the appropriate assay separately, and at present, none
of the standard cytotoxic methods can be universally applied to determine antivenom potency.
PB  - Termedia Publishing
T2  - Central European Journal of Immunology
T1  - Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom
EP  - 104
IS  - 2
SP  - 94
VL  - 49
DO  - 10.5114/ceji.2024.142417
ER  - 

@article{
author = "Lukić, Ivana and Blagojević, Veljko and Minić, Rajna and Ivanović, Saša and Borozan, Sunčica and Ćupić, Vitomir and Živković, Irena",
year = "2024",
abstract = "that can most closely evaluate the biological activity of the drug of interest. For testing the potency
of antivenom, these are the methods used to assess cytotoxicity. The aim of this study was to evaluate
the most commonly used cytotoxicity methods for determining the protective potency of the antivenom
Viekvin, which neutralizes Vipera ammodytes venom.
Material and methods: The selected methods are based on different biological mechanisms: MTT
assay, based on the activity of cell oxidoreductase enzymes; crystal violet staining, based on the degree
of cell adhesion; trypan blue staining, based on cell membrane permeability, and propidium iodide
staining, based on measurement of nucleic acids of dead cells. The pro-apoptotic effect of the venom
was also determined with annexin V staining.
Results: The IC50 value of V. ammodytes venom obtained by these methods was very similar, while
the EC50 values differed significantly.
Conclusions: We concluded that the choice of the method used to measure the anticytotoxic antivenom
potency depends on the immunogenicity of the venom components that cause cell death; for each
venom/antivenom pair, it is necessary to select the appropriate assay separately, and at present, none
of the standard cytotoxic methods can be universally applied to determine antivenom potency.",
publisher = "Termedia Publishing",
journal = "Central European Journal of Immunology",
title = "Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom",
pages = "104-94",
number = "2",
volume = "49",
doi = "10.5114/ceji.2024.142417"
}

Lukić, I., Blagojević, V., Minić, R., Ivanović, S., Borozan, S., Ćupić, V.,& Živković, I.. (2024). Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom. in Central European Journal of Immunology
Termedia Publishing., 49(2), 94-104.
https://doi.org/10.5114/ceji.2024.142417

Lukić I, Blagojević V, Minić R, Ivanović S, Borozan S, Ćupić V, Živković I. Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom. in Central European Journal of Immunology. 2024;49(2):94-104.
doi:10.5114/ceji.2024.142417 .

Lukić, Ivana, Blagojević, Veljko, Minić, Rajna, Ivanović, Saša, Borozan, Sunčica, Ćupić, Vitomir, Živković, Irena, "Comparison of cytotoxicity methods for studying Vipera ammodytes venom and the anticytotoxic potency of antivenom" in Central European Journal of Immunology, 49, no. 2 (2024):94-104,
https://doi.org/10.5114/ceji.2024.142417 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Veljović, Katarina
AU  - Radojević, Dušan
AU  - Brdarić, Emilija
AU  - Stevanović, Dušan
AU  - Živković, Milica
AU  - Kojić, Milan
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/975
AB  - This study aimed to investigate the probiogenomic features of artisanal bacteriocin-producing Enterococcus faecium BGPAS1-3 and the use of the improved pMALc5HisEk expression vector for overexpressing class II bacteriocins and the application of purified bacteriocin 31 in a milk model as a preservative against L. monocytogenes. The BGPAS1-3 strain was isolated from traditional fresh soft cheese manufactured in households on a small scale in rural locations surrounding Pale Mountain City in Bosnia and Herzegovina. The whole-genome sequencing approach and bioinformatics analyses revealed that the strain BGPAS1-3 was non-pathogenic to humans. The presence of bacteriocin operons suggested the ability of the isolate to suppress the growth of pathogens. Coding regions for three maturated bacteriocins (bacteriocin 31, bacteriocin 32, and enterocin P) produced by BGPAS1-3 were amplified and expressed in Escherichia coli ER2523 using the pMALc5HisEk system. All three bacteriocins were successfully overexpressed and purified after enterokinase cleavage but showed different antimicrobial activity. Bacteriocin 31 showed significantly stronger antimicrobial activity compared with bacteriocin 32. It was the only one that proved to be suitable for use as a food preservative against L. monocytogenes in a milk model.
PB  - MDPI
T2  - Foods
T1  - Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin
IS  - 16
SP  - 2637
VL  - 13
DO  - 10.3390/foods13162637
ER  - 

@article{
author = "Popović, Nikola and Veljović, Katarina and Radojević, Dušan and Brdarić, Emilija and Stevanović, Dušan and Živković, Milica and Kojić, Milan",
year = "2024",
abstract = "This study aimed to investigate the probiogenomic features of artisanal bacteriocin-producing Enterococcus faecium BGPAS1-3 and the use of the improved pMALc5HisEk expression vector for overexpressing class II bacteriocins and the application of purified bacteriocin 31 in a milk model as a preservative against L. monocytogenes. The BGPAS1-3 strain was isolated from traditional fresh soft cheese manufactured in households on a small scale in rural locations surrounding Pale Mountain City in Bosnia and Herzegovina. The whole-genome sequencing approach and bioinformatics analyses revealed that the strain BGPAS1-3 was non-pathogenic to humans. The presence of bacteriocin operons suggested the ability of the isolate to suppress the growth of pathogens. Coding regions for three maturated bacteriocins (bacteriocin 31, bacteriocin 32, and enterocin P) produced by BGPAS1-3 were amplified and expressed in Escherichia coli ER2523 using the pMALc5HisEk system. All three bacteriocins were successfully overexpressed and purified after enterokinase cleavage but showed different antimicrobial activity. Bacteriocin 31 showed significantly stronger antimicrobial activity compared with bacteriocin 32. It was the only one that proved to be suitable for use as a food preservative against L. monocytogenes in a milk model.",
publisher = "MDPI",
journal = "Foods",
title = "Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin",
number = "16",
pages = "2637",
volume = "13",
doi = "10.3390/foods13162637"
}

Popović, N., Veljović, K., Radojević, D., Brdarić, E., Stevanović, D., Živković, M.,& Kojić, M.. (2024). Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin. in Foods
MDPI., 13(16), 2637.
https://doi.org/10.3390/foods13162637

Popović N, Veljović K, Radojević D, Brdarić E, Stevanović D, Živković M, Kojić M. Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin. in Foods. 2024;13(16):2637.
doi:10.3390/foods13162637 .

Popović, Nikola, Veljović, Katarina, Radojević, Dušan, Brdarić, Emilija, Stevanović, Dušan, Živković, Milica, Kojić, Milan, "Insight into the Probiogenomic Potential of Enterococcus faecium BGPAS1-3 and Application of a Potent Thermostable Bacteriocin" in Foods, 13, no. 16 (2024):2637,
https://doi.org/10.3390/foods13162637 . .

TY  - DATA
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Vasić, Marko
AU  - Bogdanović, Andrija
AU  - Blagojević, Veljko
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/974
AB  - We provide one excel (with eight sheets) and 1 jpg file. Data files named ’Raw data_Fig. 1,2,3,5,6,7,8,9.xls’ and ’Raw data _histology_Fig.4.jpg’ contain raw data on analyzed traits in the submitted manuscript. Data files named ’Raw data_Fig. 1,2,3,5,6,7,8,9.xls’  consists of 8 sheets. The first sheet named ’Fig. 1’ contains data contains data showing the number of AO and DA rats (A) and the proportion of their survival (B) over 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The second sheet named ’Fig. 2’ contains data  showing length of colon segment  of AO and DA rats affected by necrosis or hyperemia/edema  7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The third sheet named ’Fig. 3’ contains data  showing consumption of food (A), water (B) and weight loss (C) of AO and DA rats following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The fourth sheet named ’Fig. 5’ contains data showing the level of NO, urea (A), cytokines (B) and relative IL-17/IFN-γ balance of production, in colon tissue homogenates of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The fifth sheet named ’Fig. 6’ contains data showing the expression of CD11b and CD4 (B), the proportion of HIS48hiCD43+ cells among CD11bintCD4low cells, and the proportion of CD163+MHCIIlo and CD163-MHCIIhi cells among resident CD11bhi CD4hi cells (C) in peritoneal washing of  AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The sixth sheet name ’Fig. 7’ contains data showing the concentration of  NO, Urea  in unstimulated  (A), and  of TNF, IL-6 and IL-10 in LPS-stimulated (B) adherent peritoneal cells  of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The seventh sheet name ’Fig. 8’ contains data showing

the proportion of CD4+, CD4+CD25+, CD8+ and CD45RA+ cells in mesenteric lymph nodes (A), the concentration of  IL-17 and IFN- IFN-γ (B) and the relative balance of  IL-17/IFN-γ production (C) in Con A-stimulated mesenteric lymph node cells of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The eight sheet named ’Fig. 9’ contains data showing the concentration of proteins  in sera of  AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.) (A), the  levels of specific anti-E. coli antibodies of IgG1, IgG2a,  IgG2b and IgA classes in sera of saline-injected AO and DA rats  (B), and the  levels of specific anti-E. coli antibodies of IgG1 (C), IgG2a (D),  IgG2b (E) and IgA (F) classes in sera of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). Data files names ’Raw data _histology_Fig.4.jpg’ contains original photomicrographs od HE-stained slides with colon tissue obtained from AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.).
T1  - Raw data for the article: Stanojević, S.; Vujić, V.; Ćuruvija, I.; Vasić, M.; Bogdanović, A.; Blagojević, V. Inflammation Makes All the Difference: Chemically-Induced Colitis in Two Inbred Rat Strains as Possible Model for Search of Therapy Targeting Inflammatory Processes. 2024.
UR  - https://hdl.handle.net/21.15107/rcub_intor_974
ER  - 

@misc{
author = "Stanojević, Stanislava and Vujić, Vesna and Ćuruvija, Ivana and Vasić, Marko and Bogdanović, Andrija and Blagojević, Veljko",
year = "2024",
abstract = "We provide one excel (with eight sheets) and 1 jpg file. Data files named ’Raw data_Fig. 1,2,3,5,6,7,8,9.xls’ and ’Raw data _histology_Fig.4.jpg’ contain raw data on analyzed traits in the submitted manuscript. Data files named ’Raw data_Fig. 1,2,3,5,6,7,8,9.xls’  consists of 8 sheets. The first sheet named ’Fig. 1’ contains data contains data showing the number of AO and DA rats (A) and the proportion of their survival (B) over 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The second sheet named ’Fig. 2’ contains data  showing length of colon segment  of AO and DA rats affected by necrosis or hyperemia/edema  7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The third sheet named ’Fig. 3’ contains data  showing consumption of food (A), water (B) and weight loss (C) of AO and DA rats following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The fourth sheet named ’Fig. 5’ contains data showing the level of NO, urea (A), cytokines (B) and relative IL-17/IFN-γ balance of production, in colon tissue homogenates of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The fifth sheet named ’Fig. 6’ contains data showing the expression of CD11b and CD4 (B), the proportion of HIS48hiCD43+ cells among CD11bintCD4low cells, and the proportion of CD163+MHCIIlo and CD163-MHCIIhi cells among resident CD11bhi CD4hi cells (C) in peritoneal washing of  AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The sixth sheet name ’Fig. 7’ contains data showing the concentration of  NO, Urea  in unstimulated  (A), and  of TNF, IL-6 and IL-10 in LPS-stimulated (B) adherent peritoneal cells  of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The seventh sheet name ’Fig. 8’ contains data showing

the proportion of CD4+, CD4+CD25+, CD8+ and CD45RA+ cells in mesenteric lymph nodes (A), the concentration of  IL-17 and IFN- IFN-γ (B) and the relative balance of  IL-17/IFN-γ production (C) in Con A-stimulated mesenteric lymph node cells of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). The eight sheet named ’Fig. 9’ contains data showing the concentration of proteins  in sera of  AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.) (A), the  levels of specific anti-E. coli antibodies of IgG1, IgG2a,  IgG2b and IgA classes in sera of saline-injected AO and DA rats  (B), and the  levels of specific anti-E. coli antibodies of IgG1 (C), IgG2a (D),  IgG2b (E) and IgA (F) classes in sera of AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.). Data files names ’Raw data _histology_Fig.4.jpg’ contains original photomicrographs od HE-stained slides with colon tissue obtained from AO and DA rats 7 days following intrarectal instillation of 300 µl of saline, ethanol and TNBS (10 and 40 mg/kg b.w.).",
title = "Raw data for the article: Stanojević, S.; Vujić, V.; Ćuruvija, I.; Vasić, M.; Bogdanović, A.; Blagojević, V. Inflammation Makes All the Difference: Chemically-Induced Colitis in Two Inbred Rat Strains as Possible Model for Search of Therapy Targeting Inflammatory Processes. 2024.",
url = "https://hdl.handle.net/21.15107/rcub_intor_974"
}

Stanojević, S., Vujić, V., Ćuruvija, I., Vasić, M., Bogdanović, A.,& Blagojević, V.. (2024). Raw data for the article: Stanojević, S.; Vujić, V.; Ćuruvija, I.; Vasić, M.; Bogdanović, A.; Blagojević, V. Inflammation Makes All the Difference: Chemically-Induced Colitis in Two Inbred Rat Strains as Possible Model for Search of Therapy Targeting Inflammatory Processes. 2024.. .
https://hdl.handle.net/21.15107/rcub_intor_974

Stanojević S, Vujić V, Ćuruvija I, Vasić M, Bogdanović A, Blagojević V. Raw data for the article: Stanojević, S.; Vujić, V.; Ćuruvija, I.; Vasić, M.; Bogdanović, A.; Blagojević, V. Inflammation Makes All the Difference: Chemically-Induced Colitis in Two Inbred Rat Strains as Possible Model for Search of Therapy Targeting Inflammatory Processes. 2024.. 2024;.
https://hdl.handle.net/21.15107/rcub_intor_974 .

Stanojević, Stanislava, Vujić, Vesna, Ćuruvija, Ivana, Vasić, Marko, Bogdanović, Andrija, Blagojević, Veljko, "Raw data for the article: Stanojević, S.; Vujić, V.; Ćuruvija, I.; Vasić, M.; Bogdanović, A.; Blagojević, V. Inflammation Makes All the Difference: Chemically-Induced Colitis in Two Inbred Rat Strains as Possible Model for Search of Therapy Targeting Inflammatory Processes. 2024." (2024),
https://hdl.handle.net/21.15107/rcub_intor_974 .

TY  - JOUR
AU  - Babić, Tamara
AU  - Ugrin, Milena
AU  - Jeremić, Sanja
AU  - Kojić, Milan
AU  - Dinić, Jelena
AU  - Banović Đeri, Bojana
AU  - Zodiakis, Jerome
AU  - Nikolić, Aleksandra
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/958
AB  - ackground: The pervasive role of alternative promoters in context-specific isoform expression and
the importance of promoter choice over its level of transcriptional activity have been recently implied
based on pan-cancer in silico studies. We aimed to explore this phenomenon at the cellular level on the
example of a major tumor suppressor SMAD4 in search of molecular mechanisms in colorectal cancer
that could be exploited for novel biomarkers or therapeutic approaches.
Methods: Multi-omics technologies, in silico tools and in vitro functional assays were applied to analyze
the transcripts expression and the alternative promoters’ function of the SMAD4 gene in colon cell lines
HCEC-1CT, HCT116, DLD-1, SW480 and SW620.
Results: High expression of the transcript SMAD4-213 emerged as a hallmark of colon cancer cells,
while in silico tools point to its possible additional role and potential for sponging miRNAs. Based on the
observed dysregulation of SMAD4-209 and SMAD4-213 in malignant vs. non-malignant colon cells, we
propose that their expression ratio might be a solid biomarker candidate for colorectal cancer detection.
Conclusions: A differential pattern of the respective promoters’ activity was observed that corresponds
to the expression of transcripts, confirming the role of alternative promoters in context-specific isoform
expression. The investigated SMAD4 promoters and transcripts harbor translational potential that should
be further investigated.
PB  - Ivyspring International Publisher
T2  - Journal of Cancer
T1  - Dysregulation of transcripts SMAD4-209 and SMAD4-213 and their respective promoters in colon cancer cell lines
EP  - 5131
IS  - 15
SP  - 5118
VL  - 15
DO  - 10.7150/jca.98911
ER  - 

@article{
author = "Babić, Tamara and Ugrin, Milena and Jeremić, Sanja and Kojić, Milan and Dinić, Jelena and Banović Đeri, Bojana and Zodiakis, Jerome and Nikolić, Aleksandra",
year = "2024",
abstract = "ackground: The pervasive role of alternative promoters in context-specific isoform expression and
the importance of promoter choice over its level of transcriptional activity have been recently implied
based on pan-cancer in silico studies. We aimed to explore this phenomenon at the cellular level on the
example of a major tumor suppressor SMAD4 in search of molecular mechanisms in colorectal cancer
that could be exploited for novel biomarkers or therapeutic approaches.
Methods: Multi-omics technologies, in silico tools and in vitro functional assays were applied to analyze
the transcripts expression and the alternative promoters’ function of the SMAD4 gene in colon cell lines
HCEC-1CT, HCT116, DLD-1, SW480 and SW620.
Results: High expression of the transcript SMAD4-213 emerged as a hallmark of colon cancer cells,
while in silico tools point to its possible additional role and potential for sponging miRNAs. Based on the
observed dysregulation of SMAD4-209 and SMAD4-213 in malignant vs. non-malignant colon cells, we
propose that their expression ratio might be a solid biomarker candidate for colorectal cancer detection.
Conclusions: A differential pattern of the respective promoters’ activity was observed that corresponds
to the expression of transcripts, confirming the role of alternative promoters in context-specific isoform
expression. The investigated SMAD4 promoters and transcripts harbor translational potential that should
be further investigated.",
publisher = "Ivyspring International Publisher",
journal = "Journal of Cancer",
title = "Dysregulation of transcripts SMAD4-209 and SMAD4-213 and their respective promoters in colon cancer cell lines",
pages = "5131-5118",
number = "15",
volume = "15",
doi = "10.7150/jca.98911"
}

Babić, T., Ugrin, M., Jeremić, S., Kojić, M., Dinić, J., Banović Đeri, B., Zodiakis, J.,& Nikolić, A.. (2024). Dysregulation of transcripts SMAD4-209 and SMAD4-213 and their respective promoters in colon cancer cell lines. in Journal of Cancer
Ivyspring International Publisher., 15(15), 5118-5131.
https://doi.org/10.7150/jca.98911

Babić T, Ugrin M, Jeremić S, Kojić M, Dinić J, Banović Đeri B, Zodiakis J, Nikolić A. Dysregulation of transcripts SMAD4-209 and SMAD4-213 and their respective promoters in colon cancer cell lines. in Journal of Cancer. 2024;15(15):5118-5131.
doi:10.7150/jca.98911 .

Babić, Tamara, Ugrin, Milena, Jeremić, Sanja, Kojić, Milan, Dinić, Jelena, Banović Đeri, Bojana, Zodiakis, Jerome, Nikolić, Aleksandra, "Dysregulation of transcripts SMAD4-209 and SMAD4-213 and their respective promoters in colon cancer cell lines" in Journal of Cancer, 15, no. 15 (2024):5118-5131,
https://doi.org/10.7150/jca.98911 . .

TY  - JOUR
AU  - Okuka, Nina
AU  - Milinković, Neda
AU  - Veličković, Ksenija
AU  - Polovina, Snežana
AU  - Šumarac-Dumanović, Mirjana
AU  - Minić, Rajna
AU  - Korčok, Davor
AU  - Djordjevic, Brižita
AU  - Ivanović, Nevena Dj.
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/891
AB  - Obesity is often accompanied by low-grade chronic inflammation and metabolic syndrome. It has been established that microbiota influences many physiological processes, including the development of obesity, and dysbiosis has been observed in obese individuals. In this study, we aimed to evaluate the impact of a new probiotic formulation, containing two probiotic strains and the bioactive compound octacosanol, on body weight, metabolic parameters, and concentrations of certain adipocytokines and appetite-regulating hormones in obese women. This double blind placebo-controlled supplementary intervention study included twenty-five women in the intervention group and twenty-three in the placebo group, and it lasted 12 weeks. Daily oral supplementation included 7 × 1010 CFU of Lactiplantibacillus plantarum 299v (DSM9843), 5 × 109 CFU of Saccharomyces cerevisiae var. boulardii (DBVPG6763), and 40 mg of octacosanol or placebo. Body weight, metabolic parameters, adipocytokines, and appetite-regulating hormones were assessed before (T0) and after the intervention (T1). After the intervention, significantly lower median concentrations of CRP (p = 0.005) and IL-6 (p = 0.012) were measured in the intervention group than the baseline, while the median concentrations of ghrelin (p = 0.026) and HDL-cholesterol (p = 0.03) were significantly increased. The intervention group had lower CRP levels (p = 0.023) and higher ghrelin levels (p = 0.006) than the placebo group. Significant changes in BMI between groups were not observed. In summary, although the new probiotic formulation showed beneficial effects on IL-6, CRP, HDL, and ghrelin levels, its potential effects on regulating triglyceride, insulin, and glucose levels require further studies before the novel dietary intervention could be considered a useful adjuvant therapy and an effective strategy for the management of obesity and obesity-associated comorbidities.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Beneficial effects of a new probiotic formulation on adipocytokines, appetite-regulating hormones, and metabolic parameters in obese women
IS  - 14
SP  - 7658
VL  - 15
DO  - 10.1039/D4FO01269K
ER  - 

@article{
author = "Okuka, Nina and Milinković, Neda and Veličković, Ksenija and Polovina, Snežana and Šumarac-Dumanović, Mirjana and Minić, Rajna and Korčok, Davor and Djordjevic, Brižita and Ivanović, Nevena Dj.",
year = "2024",
abstract = "Obesity is often accompanied by low-grade chronic inflammation and metabolic syndrome. It has been established that microbiota influences many physiological processes, including the development of obesity, and dysbiosis has been observed in obese individuals. In this study, we aimed to evaluate the impact of a new probiotic formulation, containing two probiotic strains and the bioactive compound octacosanol, on body weight, metabolic parameters, and concentrations of certain adipocytokines and appetite-regulating hormones in obese women. This double blind placebo-controlled supplementary intervention study included twenty-five women in the intervention group and twenty-three in the placebo group, and it lasted 12 weeks. Daily oral supplementation included 7 × 1010 CFU of Lactiplantibacillus plantarum 299v (DSM9843), 5 × 109 CFU of Saccharomyces cerevisiae var. boulardii (DBVPG6763), and 40 mg of octacosanol or placebo. Body weight, metabolic parameters, adipocytokines, and appetite-regulating hormones were assessed before (T0) and after the intervention (T1). After the intervention, significantly lower median concentrations of CRP (p = 0.005) and IL-6 (p = 0.012) were measured in the intervention group than the baseline, while the median concentrations of ghrelin (p = 0.026) and HDL-cholesterol (p = 0.03) were significantly increased. The intervention group had lower CRP levels (p = 0.023) and higher ghrelin levels (p = 0.006) than the placebo group. Significant changes in BMI between groups were not observed. In summary, although the new probiotic formulation showed beneficial effects on IL-6, CRP, HDL, and ghrelin levels, its potential effects on regulating triglyceride, insulin, and glucose levels require further studies before the novel dietary intervention could be considered a useful adjuvant therapy and an effective strategy for the management of obesity and obesity-associated comorbidities.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Beneficial effects of a new probiotic formulation on adipocytokines, appetite-regulating hormones, and metabolic parameters in obese women",
number = "14",
pages = "7658",
volume = "15",
doi = "10.1039/D4FO01269K"
}

Okuka, N., Milinković, N., Veličković, K., Polovina, S., Šumarac-Dumanović, M., Minić, R., Korčok, D., Djordjevic, B.,& Ivanović, N. Dj.. (2024). Beneficial effects of a new probiotic formulation on adipocytokines, appetite-regulating hormones, and metabolic parameters in obese women. in Food & Function
Royal Society of Chemistry., 15(14), 7658.
https://doi.org/10.1039/D4FO01269K

Okuka N, Milinković N, Veličković K, Polovina S, Šumarac-Dumanović M, Minić R, Korčok D, Djordjevic B, Ivanović ND. Beneficial effects of a new probiotic formulation on adipocytokines, appetite-regulating hormones, and metabolic parameters in obese women. in Food & Function. 2024;15(14):7658.
doi:10.1039/D4FO01269K .

Okuka, Nina, Milinković, Neda, Veličković, Ksenija, Polovina, Snežana, Šumarac-Dumanović, Mirjana, Minić, Rajna, Korčok, Davor, Djordjevic, Brižita, Ivanović, Nevena Dj., "Beneficial effects of a new probiotic formulation on adipocytokines, appetite-regulating hormones, and metabolic parameters in obese women" in Food & Function, 15, no. 14 (2024):7658,
https://doi.org/10.1039/D4FO01269K . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Arsenović-Ranin, Nevena
AU  - Živković, Irena
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Dragačević, Luka
AU  - Kovačević, Ana
AU  - Kotur-Stevuljević, Jelena
AU  - Leposavić, Gordana
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/893
AB  - Notwithstanding prevalence gaps in micronutrients supporting immune functions, the significance of their deficits/supplementation for the efficacy of vaccines is underinvestigated. Thus, the influence of supplementation combining vitamins C and D, zinc, selenium, manganese, and N-acetyl cysteine on immune correlates/surrogates of protection conferred by a quadrivalent influenza vaccine (QIV) in mice was investigated. The supplementation starting 5 days before the first of two QIV injections given 28 days apart increased the serum titres of total and neutralizing IgG against each of four influenza strains from QIV. Accordingly, the frequencies of germinal center B cells, follicular CD4+ T helper (Th) cells, and IL-21-producing Th cells increased in secondary lymphoid organs (SLOs). Additionally, the supplementation improved already increased IgG response to the second QIV injection by augmenting not only neutralizing antibody production, but also IgG2a response, which is important for virus clearance, through favoring Th1 differentiation as indicated by Th1 (IFN-γ)/Th2 (IL-4) signature cytokine level ratio upon QIV restimulation in SLO cell cultures. This most likely partly reflected antioxidant action of the supplement as indicated by splenic redox status analyses. Thus, the study provides a solid scientific background for further research aimed at repurposing the use of this safe and inexpensive micronutrient combination to improve response to the influenza vaccine.
PB  - MDPI
T2  - Vaccines
T1  - Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation
IS  - 7
SP  - 743
VL  - 12
DO  - 10.3390/vaccines12070743
ER  - 

@article{
author = "Bufan, Biljana and Arsenović-Ranin, Nevena and Živković, Irena and Ćuruvija, Ivana and Blagojević, Veljko and Dragačević, Luka and Kovačević, Ana and Kotur-Stevuljević, Jelena and Leposavić, Gordana",
year = "2024",
abstract = "Notwithstanding prevalence gaps in micronutrients supporting immune functions, the significance of their deficits/supplementation for the efficacy of vaccines is underinvestigated. Thus, the influence of supplementation combining vitamins C and D, zinc, selenium, manganese, and N-acetyl cysteine on immune correlates/surrogates of protection conferred by a quadrivalent influenza vaccine (QIV) in mice was investigated. The supplementation starting 5 days before the first of two QIV injections given 28 days apart increased the serum titres of total and neutralizing IgG against each of four influenza strains from QIV. Accordingly, the frequencies of germinal center B cells, follicular CD4+ T helper (Th) cells, and IL-21-producing Th cells increased in secondary lymphoid organs (SLOs). Additionally, the supplementation improved already increased IgG response to the second QIV injection by augmenting not only neutralizing antibody production, but also IgG2a response, which is important for virus clearance, through favoring Th1 differentiation as indicated by Th1 (IFN-γ)/Th2 (IL-4) signature cytokine level ratio upon QIV restimulation in SLO cell cultures. This most likely partly reflected antioxidant action of the supplement as indicated by splenic redox status analyses. Thus, the study provides a solid scientific background for further research aimed at repurposing the use of this safe and inexpensive micronutrient combination to improve response to the influenza vaccine.",
publisher = "MDPI",
journal = "Vaccines",
title = "Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation",
number = "7",
pages = "743",
volume = "12",
doi = "10.3390/vaccines12070743"
}

Bufan, B., Arsenović-Ranin, N., Živković, I., Ćuruvija, I., Blagojević, V., Dragačević, L., Kovačević, A., Kotur-Stevuljević, J.,& Leposavić, G.. (2024). Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation. in Vaccines
MDPI., 12(7), 743.
https://doi.org/10.3390/vaccines12070743

Bufan B, Arsenović-Ranin N, Živković I, Ćuruvija I, Blagojević V, Dragačević L, Kovačević A, Kotur-Stevuljević J, Leposavić G. Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation. in Vaccines. 2024;12(7):743.
doi:10.3390/vaccines12070743 .

Bufan, Biljana, Arsenović-Ranin, Nevena, Živković, Irena, Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Kovačević, Ana, Kotur-Stevuljević, Jelena, Leposavić, Gordana, "Modulation of T-Cell-Dependent Humoral Immune Response to Influenza Vaccine by Multiple Antioxidant/Immunomodulatory Micronutrient Supplementation" in Vaccines, 12, no. 7 (2024):743,
https://doi.org/10.3390/vaccines12070743 . .

TY  - JOUR
AU  - Virijević, Katarina
AU  - Živanović, Marko
AU  - Pavić, Jelena
AU  - Dragačević, Luka
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Živanović, Suzana
AU  - Milenković, Strahinja
AU  - Radojević, Ivana
AU  - Filipović, Nenad
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/892
AB  - Recent advances in regenerative medicine provide encouraging strategies to produce artificial skin substitutes. Gelatin scaffolds are successfully used as wound-dressing materials due to their superior properties, such as biocompatibility and the ability to mimic the extracellular matrix of the surrounding environment. In this study, five gelatin combination solutions were prepared and successfully electrospun using an electrospinning technique. After careful screening, the optimal concentration of the most promising combination was selected for further investigation. The obtained scaffolds were crosslinked with 25% glutaraldehyde vapor and characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy. The incorporation of antibiotic agents such as ciprofloxacin hydrochloride and gentamicin sulfate into gelatin membranes improved the already existing antibacterial properties of antibiotic-free gelatin scaffolds against Pseudomonas aeruginosa and Staphylococcus aureus. Also, the outcomes from the in vivo model study revealed that skin regeneration was significantly accelerated with gelatin/ciprofloxacin scaffold treatment. Moreover, the gelatin nanofibers were found to strongly promote the neoangiogenic process in the in vivo chick embryo chorioallantoic membrane assay. Finally, the combination of gelatin’s extracellular matrix and antibacterial agents in the scaffold suggests its potential for effective wound-healing treatments, emphasizing the importance of gelatin scaffolds in tissue engineering.
T2  - Pharmaceuticals
T1  - Electrospun Gelatin Scaffolds with Incorporated Antibiotics for Skin Wound Healing
IS  - 7
SP  - 851
VL  - 17
DO  - 10.3390/ph17070851
ER  - 

@article{
author = "Virijević, Katarina and Živanović, Marko and Pavić, Jelena and Dragačević, Luka and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Živanović, Suzana and Milenković, Strahinja and Radojević, Ivana and Filipović, Nenad",
year = "2024",
abstract = "Recent advances in regenerative medicine provide encouraging strategies to produce artificial skin substitutes. Gelatin scaffolds are successfully used as wound-dressing materials due to their superior properties, such as biocompatibility and the ability to mimic the extracellular matrix of the surrounding environment. In this study, five gelatin combination solutions were prepared and successfully electrospun using an electrospinning technique. After careful screening, the optimal concentration of the most promising combination was selected for further investigation. The obtained scaffolds were crosslinked with 25% glutaraldehyde vapor and characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy. The incorporation of antibiotic agents such as ciprofloxacin hydrochloride and gentamicin sulfate into gelatin membranes improved the already existing antibacterial properties of antibiotic-free gelatin scaffolds against Pseudomonas aeruginosa and Staphylococcus aureus. Also, the outcomes from the in vivo model study revealed that skin regeneration was significantly accelerated with gelatin/ciprofloxacin scaffold treatment. Moreover, the gelatin nanofibers were found to strongly promote the neoangiogenic process in the in vivo chick embryo chorioallantoic membrane assay. Finally, the combination of gelatin’s extracellular matrix and antibacterial agents in the scaffold suggests its potential for effective wound-healing treatments, emphasizing the importance of gelatin scaffolds in tissue engineering.",
journal = "Pharmaceuticals",
title = "Electrospun Gelatin Scaffolds with Incorporated Antibiotics for Skin Wound Healing",
number = "7",
pages = "851",
volume = "17",
doi = "10.3390/ph17070851"
}

Virijević, K., Živanović, M., Pavić, J., Dragačević, L., Ljujić, B., Miletić Kovačević, M., Papić, M., Živanović, S., Milenković, S., Radojević, I.,& Filipović, N.. (2024). Electrospun Gelatin Scaffolds with Incorporated Antibiotics for Skin Wound Healing. in Pharmaceuticals, 17(7), 851.
https://doi.org/10.3390/ph17070851

Virijević K, Živanović M, Pavić J, Dragačević L, Ljujić B, Miletić Kovačević M, Papić M, Živanović S, Milenković S, Radojević I, Filipović N. Electrospun Gelatin Scaffolds with Incorporated Antibiotics for Skin Wound Healing. in Pharmaceuticals. 2024;17(7):851.
doi:10.3390/ph17070851 .

Virijević, Katarina, Živanović, Marko, Pavić, Jelena, Dragačević, Luka, Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Živanović, Suzana, Milenković, Strahinja, Radojević, Ivana, Filipović, Nenad, "Electrospun Gelatin Scaffolds with Incorporated Antibiotics for Skin Wound Healing" in Pharmaceuticals, 17, no. 7 (2024):851,
https://doi.org/10.3390/ph17070851 . .

TY  - JOUR
AU  - Trivić, Tijana
AU  - Blagojević, Gordan
AU  - Živančević-Simonović, Snežana
AU  - Janjušević, Ana
AU  - Dragačević, Luka
AU  - Burazer, Lidija
AU  - Prodić, Ivana
AU  - Minić, Rajna
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/889
AB  - Porcine thyroglobulin was important in the discovery of alpha-Gal allergy. Here, the linkage of porcine thyroglobulin-specific IgE with IgE positivity to routinely assessed allergens and to the incoming diagnosis within a population of suspected atopic individuals is explored. IgE, IgA, total IgG and IgG subclasses to porcine thyroglobulin, IgE to bovine, human thyroglobulin and meat extract were measured with ELISA. The following correlations were observed in IgE binding to porcine and bovine thyroglobulin (r = 0.910, p = 1x10−17), porcine and human thyroglobulin (r = 0.635, p = 4x10−6), human and bovine thyroglobulin (r = 0.746, p = 6x10−9) and porcine thyroglobulin and meat extract (r = 0.482, p = 0.0009). Only one out of ten samples which showed binding to porcine thyroglobulin in ELISA tested positive with ImmunoCAP alpha-Gal, implying different epitope/s. Increased IgE binding was detected towards a more electronegative fraction of porcine thyroglobulin separated according to charge and the binding could be partially inhibited by galactose. Anti-thyroglobulin IgE was found in 29.7% of the population, in subjects who were significantly younger, p < 0.0001 and it occurred more frequently in patients referred for testing penicillin specific IgE (OR 2.48, p = 0.0059) and were negative. IgE specific to porcine, bovine and possibly human thyroglobulin may be implicated in post-infectious skin manifestation misinterpreted as penicillin allergy.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Thyroglobulin specific IgE and a possible link to suspected penicillin induced allergic skin manifestations – cross sectional study
SP  - 114795
VL  - 190
DO  - 10.1016/j.fct.2024.114795
ER  - 

@article{
author = "Trivić, Tijana and Blagojević, Gordan and Živančević-Simonović, Snežana and Janjušević, Ana and Dragačević, Luka and Burazer, Lidija and Prodić, Ivana and Minić, Rajna",
year = "2024",
abstract = "Porcine thyroglobulin was important in the discovery of alpha-Gal allergy. Here, the linkage of porcine thyroglobulin-specific IgE with IgE positivity to routinely assessed allergens and to the incoming diagnosis within a population of suspected atopic individuals is explored. IgE, IgA, total IgG and IgG subclasses to porcine thyroglobulin, IgE to bovine, human thyroglobulin and meat extract were measured with ELISA. The following correlations were observed in IgE binding to porcine and bovine thyroglobulin (r = 0.910, p = 1x10−17), porcine and human thyroglobulin (r = 0.635, p = 4x10−6), human and bovine thyroglobulin (r = 0.746, p = 6x10−9) and porcine thyroglobulin and meat extract (r = 0.482, p = 0.0009). Only one out of ten samples which showed binding to porcine thyroglobulin in ELISA tested positive with ImmunoCAP alpha-Gal, implying different epitope/s. Increased IgE binding was detected towards a more electronegative fraction of porcine thyroglobulin separated according to charge and the binding could be partially inhibited by galactose. Anti-thyroglobulin IgE was found in 29.7% of the population, in subjects who were significantly younger, p < 0.0001 and it occurred more frequently in patients referred for testing penicillin specific IgE (OR 2.48, p = 0.0059) and were negative. IgE specific to porcine, bovine and possibly human thyroglobulin may be implicated in post-infectious skin manifestation misinterpreted as penicillin allergy.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Thyroglobulin specific IgE and a possible link to suspected penicillin induced allergic skin manifestations – cross sectional study",
pages = "114795",
volume = "190",
doi = "10.1016/j.fct.2024.114795"
}

Trivić, T., Blagojević, G., Živančević-Simonović, S., Janjušević, A., Dragačević, L., Burazer, L., Prodić, I.,& Minić, R.. (2024). Thyroglobulin specific IgE and a possible link to suspected penicillin induced allergic skin manifestations – cross sectional study. in Food and Chemical Toxicology
Elsevier., 190, 114795.
https://doi.org/10.1016/j.fct.2024.114795

Trivić T, Blagojević G, Živančević-Simonović S, Janjušević A, Dragačević L, Burazer L, Prodić I, Minić R. Thyroglobulin specific IgE and a possible link to suspected penicillin induced allergic skin manifestations – cross sectional study. in Food and Chemical Toxicology. 2024;190:114795.
doi:10.1016/j.fct.2024.114795 .

Trivić, Tijana, Blagojević, Gordan, Živančević-Simonović, Snežana, Janjušević, Ana, Dragačević, Luka, Burazer, Lidija, Prodić, Ivana, Minić, Rajna, "Thyroglobulin specific IgE and a possible link to suspected penicillin induced allergic skin manifestations – cross sectional study" in Food and Chemical Toxicology, 190 (2024):114795,
https://doi.org/10.1016/j.fct.2024.114795 . .

TY  - JOUR
AU  - Virijević, Katarina
AU  - Živanović, Marko N.
AU  - Nikolić, Dalibor
AU  - Milivojević, Nevena
AU  - Pavić, Jelena
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Dragačević, Luka
AU  - Thurner, Philipp J.
AU  - Rufin, Manuel
AU  - Andriotis, Orestis G.
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Filipović, Nenad
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/872
AB  - Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.
PB  - American Chemical Society
T2  - ACS Applied Materials & Interfaces
T2  - ACS Applied Materials & InterfacesACS Appl. Mater. Interfaces
T1  - AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing
DO  - 10.1021/acsami.4c03266
ER  - 

@article{
author = "Virijević, Katarina and Živanović, Marko N. and Nikolić, Dalibor and Milivojević, Nevena and Pavić, Jelena and Morić, Ivana and Šenerović, Lidija and Dragačević, Luka and Thurner, Philipp J. and Rufin, Manuel and Andriotis, Orestis G. and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Filipović, Nenad",
year = "2024",
abstract = "Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials & Interfaces, ACS Applied Materials & InterfacesACS Appl. Mater. Interfaces",
title = "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing",
doi = "10.1021/acsami.4c03266"
}

Virijević, K., Živanović, M. N., Nikolić, D., Milivojević, N., Pavić, J., Morić, I., Šenerović, L., Dragačević, L., Thurner, P. J., Rufin, M., Andriotis, O. G., Ljujić, B., Miletić Kovačević, M., Papić, M.,& Filipović, N.. (2024). AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces
American Chemical Society..
https://doi.org/10.1021/acsami.4c03266

Virijević K, Živanović MN, Nikolić D, Milivojević N, Pavić J, Morić I, Šenerović L, Dragačević L, Thurner PJ, Rufin M, Andriotis OG, Ljujić B, Miletić Kovačević M, Papić M, Filipović N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces. 2024;.
doi:10.1021/acsami.4c03266 .

Virijević, Katarina, Živanović, Marko N., Nikolić, Dalibor, Milivojević, Nevena, Pavić, Jelena, Morić, Ivana, Šenerović, Lidija, Dragačević, Luka, Thurner, Philipp J., Rufin, Manuel, Andriotis, Orestis G., Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Filipović, Nenad, "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing" in ACS Applied Materials & Interfaces (2024),
https://doi.org/10.1021/acsami.4c03266 . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljevic, Milica
AU  - Janković, Radmila
AU  - Djuretić, Jasmina
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/867
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
PB  - MDPI
T2  - Biomedicines
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
IS  - 4
SP  - 717
VL  - 12
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Biljana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljevic, Milica and Janković, Radmila and Djuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.",
publisher = "MDPI",
journal = "Biomedicines, Biomedicines",
title = "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats",
number = "4",
pages = "717",
volume = "12",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljevic, M., Janković, R.,& Djuretić, J.. (2024). NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
MDPI., 12(4), 717.
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljevic M, Janković R, Djuretić J. NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4):717.
doi:10.3390/biomedicines12040717 .

Bufan, Biljana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljevic, Milica, Janković, Radmila, Djuretić, Jasmina, "NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024):717,
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Patiño-Guillén, Gerardo
AU  - Pešović, Jovan
AU  - Panić, Marko
AU  - Savić-Pavićević, Dušanka
AU  - Bošković, Filip
AU  - Keyser, Ulrich Felix
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/865
AB  - Transcription, a critical process in molecular biology, has found many applications in RNA synthesis, including mRNA vaccines and RNA therapeutics. However, current RNA characterization technologies suffer from amplification and enzymatic biases that lead to loss of native information. Here, we introduce a strategy to quantitatively study both transcription and RNA polymerase behaviour by sizing RNA with RNA nanotechnology and nanopores. To begin, we utilize T7 RNA polymerase to transcribe linear DNA lacking termination sequences. Surprisingly, we discover alternative transcription termination in the origin of replication sequence. Next, we employ circular DNA without transcription terminators to perform rolling circle transcription. This allows us to gain valuable insights into the processivity and transcription behaviour of RNA polymerase at the single-molecule level. Our work demonstrates how RNA nanotechnology and nanopores may be used in tandem for the direct and quantitative analysis of RNA transcripts. This methodology provides a promising pathway for accurate RNA structural mapping by enabling the study of full-length RNA transcripts at the single-molecule level.
PB  - Nature
T2  - Nature Communications
T1  - Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination
IS  - 1
SP  - 1699
VL  - 15
DO  - 10.1038/s41467-024-45968-8
ER  - 

@article{
author = "Patiño-Guillén, Gerardo and Pešović, Jovan and Panić, Marko and Savić-Pavićević, Dušanka and Bošković, Filip and Keyser, Ulrich Felix",
year = "2024",
abstract = "Transcription, a critical process in molecular biology, has found many applications in RNA synthesis, including mRNA vaccines and RNA therapeutics. However, current RNA characterization technologies suffer from amplification and enzymatic biases that lead to loss of native information. Here, we introduce a strategy to quantitatively study both transcription and RNA polymerase behaviour by sizing RNA with RNA nanotechnology and nanopores. To begin, we utilize T7 RNA polymerase to transcribe linear DNA lacking termination sequences. Surprisingly, we discover alternative transcription termination in the origin of replication sequence. Next, we employ circular DNA without transcription terminators to perform rolling circle transcription. This allows us to gain valuable insights into the processivity and transcription behaviour of RNA polymerase at the single-molecule level. Our work demonstrates how RNA nanotechnology and nanopores may be used in tandem for the direct and quantitative analysis of RNA transcripts. This methodology provides a promising pathway for accurate RNA structural mapping by enabling the study of full-length RNA transcripts at the single-molecule level.",
publisher = "Nature",
journal = "Nature Communications",
title = "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination",
number = "1",
pages = "1699",
volume = "15",
doi = "10.1038/s41467-024-45968-8"
}

Patiño-Guillén, G., Pešović, J., Panić, M., Savić-Pavićević, D., Bošković, F.,& Keyser, U. F.. (2024). Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination. in Nature Communications
Nature., 15(1), 1699.
https://doi.org/10.1038/s41467-024-45968-8

Patiño-Guillén G, Pešović J, Panić M, Savić-Pavićević D, Bošković F, Keyser UF. Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination. in Nature Communications. 2024;15(1):1699.
doi:10.1038/s41467-024-45968-8 .

Patiño-Guillén, Gerardo, Pešović, Jovan, Panić, Marko, Savić-Pavićević, Dušanka, Bošković, Filip, Keyser, Ulrich Felix, "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination" in Nature Communications, 15, no. 1 (2024):1699,
https://doi.org/10.1038/s41467-024-45968-8 . .

TY  - JOUR
AU  - Ćurčić, Jovana
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/864
AB  - Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo
SP  - 130421
DO  - 10.1016/j.ijbiomac.2024.130421
ER  - 

@article{
author = "Ćurčić, Jovana and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2024",
abstract = "Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo",
pages = "130421",
doi = "10.1016/j.ijbiomac.2024.130421"
}

Ćurčić, J., Dinić, M., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2024). A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules
Elsevier., 130421.
https://doi.org/10.1016/j.ijbiomac.2024.130421

Ćurčić J, Dinić M, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules. 2024;:130421.
doi:10.1016/j.ijbiomac.2024.130421 .

Ćurčić, Jovana, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo" in International Journal of Biological Macromolecules (2024):130421,
https://doi.org/10.1016/j.ijbiomac.2024.130421 . .

TY  - JOUR
AU  - Protić-Rosić, Isidora
AU  - Lopandić, Zorana
AU  - Popović, Dragan
AU  - Blagojević, Gordan
AU  - Gavrović-Jankulović, Marija
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/861
AB  - Novel allergen immunotherapy (AIT) approaches necessitate the use of more effective and safe therapeutics, which can be accomplished by employing novel adjuvants for improved innate immune cell activation, as well as hypoallergenic allergen forms. In this study, we investigate the immunomodulatory effects of a chimera rBet v 1a-BanLecwt (rBv1a-BLwt; Cwt) composed of the major birch pollen allergen Bet v 1a and banana lectin (BanLecwt; BLwt) and two novel chimeras, rBv1l-BLH84T (rBet v 1l-BanLecH84T; C1) and rBLH84T-Bv1l (rBanLecH84T-Bet v 1l; C2), both composed of BLH84T and hypoallergenic birch pollen allergen Bv1l in the co-culture model Caco-2/THP-1, and PBMCs from donors with birch pollen allergy. The chimeric molecules rBv1l-BLH84T (C1) and rBLH84T-Bv1l (C2) were created in silico and then produced in E. coli using recombinant DNA technology. Real-time PCR analysis of gene expression following compound treatment in the co-culture model revealed that all three chimeras have the potential to induce the anti-inflammatory cytokine IL-10 gene expression in Caco-2 cells and IFN-γ gene expression in THP-1 cells. Sandwich ELISA revealed that Cwt increased IL-10 secretion and IFN-/IL-4 levels in PBMCs from birch pollen allergic donors, whereas C1 and C2 were less effective. The findings suggest that Cwt should be analyzed further due to its potential benefit in AIT.
PB  - Elsevier
T2  - International Immunopharmacology
T1  - rBet v 1a-BanLecwt induce upregulation of IL-10 and IFN-γ gene expression in Caco-2/THP-1 co-culture and secretion of IL-10 and IFN-γ/IL-4 levels in PBMCs of birch pollen allergic donors
SP  - 111607
VL  - 129
DO  - 10.1016/j.intimp.2024.111607
ER  - 

@article{
author = "Protić-Rosić, Isidora and Lopandić, Zorana and Popović, Dragan and Blagojević, Gordan and Gavrović-Jankulović, Marija",
year = "2024",
abstract = "Novel allergen immunotherapy (AIT) approaches necessitate the use of more effective and safe therapeutics, which can be accomplished by employing novel adjuvants for improved innate immune cell activation, as well as hypoallergenic allergen forms. In this study, we investigate the immunomodulatory effects of a chimera rBet v 1a-BanLecwt (rBv1a-BLwt; Cwt) composed of the major birch pollen allergen Bet v 1a and banana lectin (BanLecwt; BLwt) and two novel chimeras, rBv1l-BLH84T (rBet v 1l-BanLecH84T; C1) and rBLH84T-Bv1l (rBanLecH84T-Bet v 1l; C2), both composed of BLH84T and hypoallergenic birch pollen allergen Bv1l in the co-culture model Caco-2/THP-1, and PBMCs from donors with birch pollen allergy. The chimeric molecules rBv1l-BLH84T (C1) and rBLH84T-Bv1l (C2) were created in silico and then produced in E. coli using recombinant DNA technology. Real-time PCR analysis of gene expression following compound treatment in the co-culture model revealed that all three chimeras have the potential to induce the anti-inflammatory cytokine IL-10 gene expression in Caco-2 cells and IFN-γ gene expression in THP-1 cells. Sandwich ELISA revealed that Cwt increased IL-10 secretion and IFN-/IL-4 levels in PBMCs from birch pollen allergic donors, whereas C1 and C2 were less effective. The findings suggest that Cwt should be analyzed further due to its potential benefit in AIT.",
publisher = "Elsevier",
journal = "International Immunopharmacology",
title = "rBet v 1a-BanLecwt induce upregulation of IL-10 and IFN-γ gene expression in Caco-2/THP-1 co-culture and secretion of IL-10 and IFN-γ/IL-4 levels in PBMCs of birch pollen allergic donors",
pages = "111607",
volume = "129",
doi = "10.1016/j.intimp.2024.111607"
}

Protić-Rosić, I., Lopandić, Z., Popović, D., Blagojević, G.,& Gavrović-Jankulović, M.. (2024). rBet v 1a-BanLecwt induce upregulation of IL-10 and IFN-γ gene expression in Caco-2/THP-1 co-culture and secretion of IL-10 and IFN-γ/IL-4 levels in PBMCs of birch pollen allergic donors. in International Immunopharmacology
Elsevier., 129, 111607.
https://doi.org/10.1016/j.intimp.2024.111607

Protić-Rosić I, Lopandić Z, Popović D, Blagojević G, Gavrović-Jankulović M. rBet v 1a-BanLecwt induce upregulation of IL-10 and IFN-γ gene expression in Caco-2/THP-1 co-culture and secretion of IL-10 and IFN-γ/IL-4 levels in PBMCs of birch pollen allergic donors. in International Immunopharmacology. 2024;129:111607.
doi:10.1016/j.intimp.2024.111607 .

Protić-Rosić, Isidora, Lopandić, Zorana, Popović, Dragan, Blagojević, Gordan, Gavrović-Jankulović, Marija, "rBet v 1a-BanLecwt induce upregulation of IL-10 and IFN-γ gene expression in Caco-2/THP-1 co-culture and secretion of IL-10 and IFN-γ/IL-4 levels in PBMCs of birch pollen allergic donors" in International Immunopharmacology, 129 (2024):111607,
https://doi.org/10.1016/j.intimp.2024.111607 . .

TY  - CONF
AU  - Pavlović, Marija
AU  - Kojić, Milan
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Vujčić, Zoran
AU  - Šokarda Slavić, Marinela
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/936
AB  - This research deals with the characterization of a thermostable endo-1,4-beta-xylanase enzyme from Anoxybacillus vranjensis ST4, a thermophilic bacterium isolated from Vranjska Banja hot spring, Serbia. The enzyme shows a high degree of identity with the same type of enzyme from other species of the genera Anoxybacillus (97%), Geobacillus (74%) and Paenibacillus (65%). The gene for endo-1,4-beta-xylanase from the thermophilic strain ST4 was cloned into the pQE_Ek expression vector and successfully expressed and purified from the Escherichia coli M15[pREP4]. The study encompasses recombinant production, purification, and the comprehensive characterization of the enzymatic properties of endo-1,4-beta-xylanase. This is the first successful overexpression, purification and characterization of a recombinant thermostable endo-1,4-beta-xylanase enzyme from Anoxybacillus. With a monomeric structure of 38.7 kDa, the enzyme demonstrates peak activity at 70°C and pH 6.5. Notably, it exhibits remarkable stability across a wide pH range and at high temperatures, rendering it suitable for diverse industrial applications. Investigation into the enzyme’s kinetic parameters, substrate specificity, and its ability to degrade xylan into high-energy value products further enhances understanding of its biotechnological potential. These findings underscore the significance of thermophilic bacteria and their thermostable enzymes in various industrial processes.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia, Book of abstracts, April 4-6, 2024, Belgrade, Serbia
T1  - Cloning, overexpression and characterization of a thermostable endo-1,4-beta-xylanase from Anoxybacillus vranjensis ST4
EP  - 31
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_intor_936
ER  - 

@conference{
author = "Pavlović, Marija and Kojić, Milan and Ristović, Marina and Stojanović, Sanja and Margetić, Aleksandra and Vujčić, Zoran and Šokarda Slavić, Marinela",
year = "2024",
abstract = "This research deals with the characterization of a thermostable endo-1,4-beta-xylanase enzyme from Anoxybacillus vranjensis ST4, a thermophilic bacterium isolated from Vranjska Banja hot spring, Serbia. The enzyme shows a high degree of identity with the same type of enzyme from other species of the genera Anoxybacillus (97%), Geobacillus (74%) and Paenibacillus (65%). The gene for endo-1,4-beta-xylanase from the thermophilic strain ST4 was cloned into the pQE_Ek expression vector and successfully expressed and purified from the Escherichia coli M15[pREP4]. The study encompasses recombinant production, purification, and the comprehensive characterization of the enzymatic properties of endo-1,4-beta-xylanase. This is the first successful overexpression, purification and characterization of a recombinant thermostable endo-1,4-beta-xylanase enzyme from Anoxybacillus. With a monomeric structure of 38.7 kDa, the enzyme demonstrates peak activity at 70°C and pH 6.5. Notably, it exhibits remarkable stability across a wide pH range and at high temperatures, rendering it suitable for diverse industrial applications. Investigation into the enzyme’s kinetic parameters, substrate specificity, and its ability to degrade xylan into high-energy value products further enhances understanding of its biotechnological potential. These findings underscore the significance of thermophilic bacteria and their thermostable enzymes in various industrial processes.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia, Book of abstracts, April 4-6, 2024, Belgrade, Serbia",
title = "Cloning, overexpression and characterization of a thermostable endo-1,4-beta-xylanase from Anoxybacillus vranjensis ST4",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_intor_936"
}

Pavlović, M., Kojić, M., Ristović, M., Stojanović, S., Margetić, A., Vujčić, Z.,& Šokarda Slavić, M.. (2024). Cloning, overexpression and characterization of a thermostable endo-1,4-beta-xylanase from Anoxybacillus vranjensis ST4. in XIII Congress of microbiologists of Serbia, Book of abstracts, April 4-6, 2024, Belgrade, Serbia
Serbian Society for Microbiology., 31-31.
https://hdl.handle.net/21.15107/rcub_intor_936

Pavlović M, Kojić M, Ristović M, Stojanović S, Margetić A, Vujčić Z, Šokarda Slavić M. Cloning, overexpression and characterization of a thermostable endo-1,4-beta-xylanase from Anoxybacillus vranjensis ST4. in XIII Congress of microbiologists of Serbia, Book of abstracts, April 4-6, 2024, Belgrade, Serbia. 2024;:31-31.
https://hdl.handle.net/21.15107/rcub_intor_936 .

Pavlović, Marija, Kojić, Milan, Ristović, Marina, Stojanović, Sanja, Margetić, Aleksandra, Vujčić, Zoran, Šokarda Slavić, Marinela, "Cloning, overexpression and characterization of a thermostable endo-1,4-beta-xylanase from Anoxybacillus vranjensis ST4" in XIII Congress of microbiologists of Serbia, Book of abstracts, April 4-6, 2024, Belgrade, Serbia (2024):31-31,
https://hdl.handle.net/21.15107/rcub_intor_936 .

TY  - JOUR
AU  - Prodić, Ivana
AU  - Minić, Rajna
AU  - Stojadinović, Marija
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/890
AB  - Grass pollen is the most common cause of pollen allergies in Europe. However, growing evidence suggests that air pollution and climate change may contribute to the rising number of allergic cases and worsening symptoms. This narrative review article aims to summarize the impacts of increased health complications based on pollution research in recent years, obtained from ecological, molecular and clinical studies to provide a new perspective on the impact of pollutants on the environment and human health. Our detailed literature review includes studies on pollution and its effect on pollen allergens, which cause allergy symptoms, but only in the case of three grass species: Dactylis glomerata, Lolium perenne and Phleum pratense.
PB  - Springer
T2  - Aerobiologia
T1  - The influence of environmental pollution on the allergenic potential of grass pollen
DO  - 10.1007/s10453-024-09829-7
ER  - 

@article{
author = "Prodić, Ivana and Minić, Rajna and Stojadinović, Marija",
year = "2024",
abstract = "Grass pollen is the most common cause of pollen allergies in Europe. However, growing evidence suggests that air pollution and climate change may contribute to the rising number of allergic cases and worsening symptoms. This narrative review article aims to summarize the impacts of increased health complications based on pollution research in recent years, obtained from ecological, molecular and clinical studies to provide a new perspective on the impact of pollutants on the environment and human health. Our detailed literature review includes studies on pollution and its effect on pollen allergens, which cause allergy symptoms, but only in the case of three grass species: Dactylis glomerata, Lolium perenne and Phleum pratense.",
publisher = "Springer",
journal = "Aerobiologia",
title = "The influence of environmental pollution on the allergenic potential of grass pollen",
doi = "10.1007/s10453-024-09829-7"
}

Prodić, I., Minić, R.,& Stojadinović, M.. (2024). The influence of environmental pollution on the allergenic potential of grass pollen. in Aerobiologia
Springer..
https://doi.org/10.1007/s10453-024-09829-7

Prodić I, Minić R, Stojadinović M. The influence of environmental pollution on the allergenic potential of grass pollen. in Aerobiologia. 2024;.
doi:10.1007/s10453-024-09829-7 .

Prodić, Ivana, Minić, Rajna, Stojadinović, Marija, "The influence of environmental pollution on the allergenic potential of grass pollen" in Aerobiologia (2024),
https://doi.org/10.1007/s10453-024-09829-7 . .

TY  - JOUR
AU  - Pavlović, Marija
AU  - Slavić, Marinela Šokarda
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Drulović, Nenad
AU  - Vujčić, Zoran
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/888
AB  - Microbial pectinolytic enzymes are important in various industries, particularly food processing. This study focuses on uncovering insights into a novel pectin lyase, BvPelB, from Bacillus velezensis 16B, with the aim of enhancing fruit juice processing. The study examines the structural and functional characteristics of pectinolytic enzyme, underscoring the critical nature of substrate specificity and enzymatic reaction mechanisms. BvPelB was successfully expressed and purified, exhibiting robust activity under alkaline conditions and thermal stability. Structural analysis revealed similarities with other pectin lyases, despite limited sequence identity. Biochemical characterization showed BvPelB's preference for highly methylated pectins and its endo-acting mode of cleavage. Treatment with BvPelB significantly increased juice yield and clarity without generating excessive methanol, making it a promising candidate for fruit juice processing. Overall, this study provides valuable insights into the enzymatic properties of BvPelB and its potential industrial applications in improving fruit juice processing efficiency and quality.
PB  - Elsevier
T2  - Food Chemistry
T1  - Unveiling novel insights into Bacillus velezensis 16B pectin lyase for improved fruit juice processing
SP  - 140030
DO  - 10.1016/j.foodchem.2024.140030
ER  - 

@article{
author = "Pavlović, Marija and Slavić, Marinela Šokarda and Kojić, Milan and Margetić, Aleksandra and Ristović, Marina and Drulović, Nenad and Vujčić, Zoran",
year = "2024",
abstract = "Microbial pectinolytic enzymes are important in various industries, particularly food processing. This study focuses on uncovering insights into a novel pectin lyase, BvPelB, from Bacillus velezensis 16B, with the aim of enhancing fruit juice processing. The study examines the structural and functional characteristics of pectinolytic enzyme, underscoring the critical nature of substrate specificity and enzymatic reaction mechanisms. BvPelB was successfully expressed and purified, exhibiting robust activity under alkaline conditions and thermal stability. Structural analysis revealed similarities with other pectin lyases, despite limited sequence identity. Biochemical characterization showed BvPelB's preference for highly methylated pectins and its endo-acting mode of cleavage. Treatment with BvPelB significantly increased juice yield and clarity without generating excessive methanol, making it a promising candidate for fruit juice processing. Overall, this study provides valuable insights into the enzymatic properties of BvPelB and its potential industrial applications in improving fruit juice processing efficiency and quality.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Unveiling novel insights into Bacillus velezensis 16B pectin lyase for improved fruit juice processing",
pages = "140030",
doi = "10.1016/j.foodchem.2024.140030"
}

Pavlović, M., Slavić, M. Š., Kojić, M., Margetić, A., Ristović, M., Drulović, N.,& Vujčić, Z.. (2024). Unveiling novel insights into Bacillus velezensis 16B pectin lyase for improved fruit juice processing. in Food Chemistry
Elsevier., 140030.
https://doi.org/10.1016/j.foodchem.2024.140030

Pavlović M, Slavić MŠ, Kojić M, Margetić A, Ristović M, Drulović N, Vujčić Z. Unveiling novel insights into Bacillus velezensis 16B pectin lyase for improved fruit juice processing. in Food Chemistry. 2024;:140030.
doi:10.1016/j.foodchem.2024.140030 .

Pavlović, Marija, Slavić, Marinela Šokarda, Kojić, Milan, Margetić, Aleksandra, Ristović, Marina, Drulović, Nenad, Vujčić, Zoran, "Unveiling novel insights into Bacillus velezensis 16B pectin lyase for improved fruit juice processing" in Food Chemistry (2024):140030,
https://doi.org/10.1016/j.foodchem.2024.140030 . .

TY  - DATA
AU  - Virijević, Katarina
AU  - Živanović, Marko N.
AU  - Nikolić, Dalibor
AU  - Milivojević, Nevena
AU  - Pavić, Jelena
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Dragačević, Luka
AU  - Thurner, Philipp J.
AU  - Rufin, Manuel
AU  - Andriotis, Orestis G.
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Filipović, Nenad
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/873
AB  - Figure S1. Publication Trends in “Electrospinning”, “Electrospinning + PCL + PEG”, “Electrospinning + Wound Healing” and “Electrospinning + Artificial Intelligence + Neural Network” Research (2001-2022) Table S1. Synonyms and Related Terms for Electrospinning in Research (2001-2022) Table S2. Input data structured for ANN – CSV data file Figure S2. Basic visualization of the dependence of output data (vertical axis) on individual input data (horizontal axis). Figure S3. Schematic representation of the neural network Figure S4. Graph of RMSE relation between training data set (blue line) and validation data set (orange line) depending on the number of neurons in the hidden layer Figure S5. Visual representation of ANN precision; the horizontal axis represents the percent of the real result, and the vertical axis represents the percent of ANN prediction; the training set (blue dots), prediction set (orange dots) Scheme S1. Electrospinning-Ready Polymer and Solvent Combinations. “Substance” is PCL or PCL combined with PEG. The substance is dissolved in mass concentrations from 17 to 28% in CHCl3 or a combination of CHCl3 and DMF. Figure S6. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 1: PCL in CHCl3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S7. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 2: PCL in CHCl3:DMF=1:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S8. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 3: PCL in CHCl3:DMF=1:3. A) 17% B) 18% C) 19% Figure S9. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 4: PCL in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% Figure S10. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 5: PCL:PEG=1:1 in CHCl3. A) 17% B) 18% C) 20% D) 21% E) 22% F) 24% G) 25% H) 26% I) 27% J) 28% Figure S11. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 6: PCL:PEG=1:1 in CHCl3:DMF=1:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S12. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 7: PCL:PEG=1:1 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% Figure S13. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 8: PCL:PEG=3:1 in CHCl3:DMF=1:1. A) 17% B) 19% C) 20% D) 21% E) 22% F) 24% G) 26% Figure S14. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 9: PCL:PEG=3:1 in CHCl3:DMF=1:3. A) 17% B) 18% C) 19% D) 22% Figure S15. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 10: PCL:PEG=3:1 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% Figure S16. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 15: PCL:PEG=1:3 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% Figure S17. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 16: PCL:PEG=7:3 in CHCl3:DMF=7:3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S18. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 17: PCL:PEG=3:1 in CHCl3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% Figure S19. The action of scaffolds bearing antibiotics on selected strains of bacteria by disk diffusion method. Figure S20. Chick Embryo CAM Assay Procedure: A) Egg selection B) Egg disinfection with 10% of iodine solution C) Inoculation and preparation for scaffold insertion D) Egg’s incubation E) Daily monitoring of embryo development and possible contamination F) Sacrifice of treated embryos and fixation with 4% PFA G) CAM membrane preparation H) Image capture and evaluation of blood vessels
PB  - American Chemical Society
T2  - ACS Applied Materials & Interfaces
T1  - Supplementary information for the article: Virijević, K.; Živanović, M. N.; Nikolić, D.; Milivojević, N.; Pavić, J.; Morić, I.; Šenerović, L.; Dragačević, L.; Thurner, P. J.; Rufin, M.; Andriotis, O. G.; Ljujić, B.; Miletić Kovačević, M.; Papić, M.; Filipović, N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. ACS Appl. Mater. Interfaces 2024. https://doi.org/10.1021/acsami.4c03266.
DO  - doi.org/10.1021/acsami.4c03266
ER  - 

@misc{
author = "Virijević, Katarina and Živanović, Marko N. and Nikolić, Dalibor and Milivojević, Nevena and Pavić, Jelena and Morić, Ivana and Šenerović, Lidija and Dragačević, Luka and Thurner, Philipp J. and Rufin, Manuel and Andriotis, Orestis G. and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Filipović, Nenad",
year = "2024",
abstract = "Figure S1. Publication Trends in “Electrospinning”, “Electrospinning + PCL + PEG”, “Electrospinning + Wound Healing” and “Electrospinning + Artificial Intelligence + Neural Network” Research (2001-2022) Table S1. Synonyms and Related Terms for Electrospinning in Research (2001-2022) Table S2. Input data structured for ANN – CSV data file Figure S2. Basic visualization of the dependence of output data (vertical axis) on individual input data (horizontal axis). Figure S3. Schematic representation of the neural network Figure S4. Graph of RMSE relation between training data set (blue line) and validation data set (orange line) depending on the number of neurons in the hidden layer Figure S5. Visual representation of ANN precision; the horizontal axis represents the percent of the real result, and the vertical axis represents the percent of ANN prediction; the training set (blue dots), prediction set (orange dots) Scheme S1. Electrospinning-Ready Polymer and Solvent Combinations. “Substance” is PCL or PCL combined with PEG. The substance is dissolved in mass concentrations from 17 to 28% in CHCl3 or a combination of CHCl3 and DMF. Figure S6. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 1: PCL in CHCl3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S7. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 2: PCL in CHCl3:DMF=1:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S8. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 3: PCL in CHCl3:DMF=1:3. A) 17% B) 18% C) 19% Figure S9. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 4: PCL in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% Figure S10. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 5: PCL:PEG=1:1 in CHCl3. A) 17% B) 18% C) 20% D) 21% E) 22% F) 24% G) 25% H) 26% I) 27% J) 28% Figure S11. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 6: PCL:PEG=1:1 in CHCl3:DMF=1:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S12. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 7: PCL:PEG=1:1 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% Figure S13. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 8: PCL:PEG=3:1 in CHCl3:DMF=1:1. A) 17% B) 19% C) 20% D) 21% E) 22% F) 24% G) 26% Figure S14. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 9: PCL:PEG=3:1 in CHCl3:DMF=1:3. A) 17% B) 18% C) 19% D) 22% Figure S15. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 10: PCL:PEG=3:1 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% Figure S16. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 15: PCL:PEG=1:3 in CHCl3:DMF=3:1. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% Figure S17. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 16: PCL:PEG=7:3 in CHCl3:DMF=7:3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% G) 23% H) 24% I) 25% J) 26% K) 27% Figure S18. Fiber Diameter Distribution Analysis of Electrospun-Derived Scaffold for Series 17: PCL:PEG=3:1 in CHCl3. A) 17% B) 18% C) 19% D) 20% E) 21% F) 22% Figure S19. The action of scaffolds bearing antibiotics on selected strains of bacteria by disk diffusion method. Figure S20. Chick Embryo CAM Assay Procedure: A) Egg selection B) Egg disinfection with 10% of iodine solution C) Inoculation and preparation for scaffold insertion D) Egg’s incubation E) Daily monitoring of embryo development and possible contamination F) Sacrifice of treated embryos and fixation with 4% PFA G) CAM membrane preparation H) Image capture and evaluation of blood vessels",
publisher = "American Chemical Society",
journal = "ACS Applied Materials & Interfaces",
title = "Supplementary information for the article: Virijević, K.; Živanović, M. N.; Nikolić, D.; Milivojević, N.; Pavić, J.; Morić, I.; Šenerović, L.; Dragačević, L.; Thurner, P. J.; Rufin, M.; Andriotis, O. G.; Ljujić, B.; Miletić Kovačević, M.; Papić, M.; Filipović, N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. ACS Appl. Mater. Interfaces 2024. https://doi.org/10.1021/acsami.4c03266.",
doi = "doi.org/10.1021/acsami.4c03266"
}

Virijević, K., Živanović, M. N., Nikolić, D., Milivojević, N., Pavić, J., Morić, I., Šenerović, L., Dragačević, L., Thurner, P. J., Rufin, M., Andriotis, O. G., Ljujić, B., Miletić Kovačević, M., Papić, M.,& Filipović, N.. (2024). Supplementary information for the article: Virijević, K.; Živanović, M. N.; Nikolić, D.; Milivojević, N.; Pavić, J.; Morić, I.; Šenerović, L.; Dragačević, L.; Thurner, P. J.; Rufin, M.; Andriotis, O. G.; Ljujić, B.; Miletić Kovačević, M.; Papić, M.; Filipović, N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. ACS Appl. Mater. Interfaces 2024. https://doi.org/10.1021/acsami.4c03266.. in ACS Applied Materials & Interfaces
American Chemical Society..
https://doi.org/doi.org/10.1021/acsami.4c03266

Virijević K, Živanović MN, Nikolić D, Milivojević N, Pavić J, Morić I, Šenerović L, Dragačević L, Thurner PJ, Rufin M, Andriotis OG, Ljujić B, Miletić Kovačević M, Papić M, Filipović N. Supplementary information for the article: Virijević, K.; Živanović, M. N.; Nikolić, D.; Milivojević, N.; Pavić, J.; Morić, I.; Šenerović, L.; Dragačević, L.; Thurner, P. J.; Rufin, M.; Andriotis, O. G.; Ljujić, B.; Miletić Kovačević, M.; Papić, M.; Filipović, N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. ACS Appl. Mater. Interfaces 2024. https://doi.org/10.1021/acsami.4c03266.. in ACS Applied Materials & Interfaces. 2024;.
doi:doi.org/10.1021/acsami.4c03266 .

Virijević, Katarina, Živanović, Marko N., Nikolić, Dalibor, Milivojević, Nevena, Pavić, Jelena, Morić, Ivana, Šenerović, Lidija, Dragačević, Luka, Thurner, Philipp J., Rufin, Manuel, Andriotis, Orestis G., Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Filipović, Nenad, "Supplementary information for the article: Virijević, K.; Živanović, M. N.; Nikolić, D.; Milivojević, N.; Pavić, J.; Morić, I.; Šenerović, L.; Dragačević, L.; Thurner, P. J.; Rufin, M.; Andriotis, O. G.; Ljujić, B.; Miletić Kovačević, M.; Papić, M.; Filipović, N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. ACS Appl. Mater. Interfaces 2024. https://doi.org/10.1021/acsami.4c03266." in ACS Applied Materials & Interfaces (2024),
https://doi.org/doi.org/10.1021/acsami.4c03266 . .

TY  - JOUR
AU  - Sinnathamby, Mary A.
AU  - Meslé, Margaux M. I.
AU  - Mook, Piers
AU  - Pebody, Richard
AU  - Bino, Silvia
AU  - Hasibra, Iris
AU  - Bakunts, Nune
AU  - Abovyan, Romella
AU  - Khachtryan, Evgenia
AU  - Redlberger‐Fritz, Monika
AU  - Mursalova, Nazifa
AU  - Aliyeva, Firuza
AU  - Vysotskaya, Veronika
AU  - Shmialiova, Natallia
AU  - Karaban, Inna
AU  - Bossuyt, Nathalie
AU  - Barbezange, Cyril
AU  - Musa, Sanja
AU  - Vukmir, Nina Rodić
AU  - Ljubović, Amela Dedeić
AU  - Baštinac, Dijana
AU  - Vladimirov, Nadezhda
AU  - Korsun, Neli
AU  - Trifonova, Ivelina
AU  - Tabain, Irena
AU  - Petrović, Goranka
AU  - Karagiannis, Christos
AU  - Haralambous, Christos
AU  - Jirincova, Helena
AU  - Kyncl, Jan
AU  - Trebbien, Ramona
AU  - Vestergaard, Lasse Skafte
AU  - Sadikova, Olga
AU  - Eero, Irina
AU  - Metsoja, Eliisa
AU  - Ikonen, Niina
AU  - Lyytikäinen, Outi
AU  - Nohynek, Hanna
AU  - Fournier, Lucie
AU  - Guerrisi, Caroline
AU  - Valette, Martine
AU  - Machablishvili, Ani
AU  - Buda, Silke
AU  - Dürrwald, Ralf
AU  - Gioula, Georgia
AU  - Mary, Emmanouil
AU  - Mellou, Kassiani
AU  - Rózsa, Mónika
AU  - Molnár, Zsuzsanna
AU  - Armannsdottir, Brynja
AU  - Aspelund, Guðrún
AU  - O’Donnell, Joan
AU  - Domegan, Lisa
AU  - Connell, Jeff
AU  - Mandelboim, Michal
AU  - Glatman‐Freedman, Aharona
AU  - Puzelli, Simona
AU  - Palamara, Anna Teresa
AU  - Maraglino, Francesco
AU  - Smagulova, Smagulova Meiramgul Kanapiyanovna
AU  - Userbayev, Aidar Sharipkhanuly
AU  - Kalaveshi, Ariana
AU  - Jakupi, Xhevat
AU  - Gunga, Zana Kaçaniku
AU  - Otorbaeva, D. S
AU  - Abdyldaeva, S. Zh
AU  - Esengeldiev, G. M
AU  - Vasiļevska, Darja
AU  - Tomašūna, Kate Karolīna
AU  - Savicka, Oksana
AU  - Gargasiene, Greta
AU  - Muralyte, Svajune
AU  - Nguyen, Trung‐Nguyen
AU  - Mossong, Joel
AU  - Abdelrahman, Tamir T.
AU  - Melillo, Tanya
AU  - Zahra, Graziella
AU  - Melillo, Jackie
AU  - Rakocevic, Bozidarka
AU  - Zekovic, Zeljka
AU  - Medenica, Sanja
AU  - de Lange, Marit
AU  - Meijer, Adam
AU  - Kochinski, Dragan
AU  - Boshevska, Golubinka
AU  - Paulsen, Trinehessevik
AU  - Bragstad, Karoline
AU  - Brydak, Lidia B.
AU  - Hallmann, Ewelina
AU  - Szymański, Karol
AU  - Rodrigues, Ana Paula
AU  - Verdasca, Nuno
AU  - Guiomar, Raquel
AU  - Druc, Alina
AU  - Apostol, Mariana
AU  - Popescu, Rodica
AU  - Popovici, Odette
AU  - Lazar, Mihaela
AU  - Komissarov, Andrey B.
AU  - Fadeev, Artem
AU  - Stolyarov, Kirill
AU  - Protić, Jelena
AU  - Avdičová, Mária
AU  - Mečochová, Adriana
AU  - Staroňová, Edita
AU  - Sočan, Maja
AU  - Prosenc, Katarina
AU  - Berginc, Nataša
AU  - Larrauri, Amparo
AU  - Mazagatos, Clara
AU  - Pozo, Francisco
AU  - Appelqvist, Emma
AU  - Carnahan, AnnaSara
AU  - Cabecinhas, Ana Rita Goncalves
AU  - Spedaliero, Tania
AU  - Jafarov, Navaruz
AU  - Safarova, Tamanno
AU  - Barotova, Barno
AU  - Kovalchuk, Tatyana
AU  - Altas, Ayse Basak
AU  - Avci, Emine
AU  - Ozdemir, Betul
AU  - Ovliyakulova, Gurbangul
AU  - Dykhanovska, Tetiana
AU  - Demchyshyna, Iryna
AU  - Koshako, Oksana
AU  - Watson, Conall
AU  - Zambon, Maria
AU  - Lakhani, Anissa
AU  - Shepherd, Samantha
AU  - Bradley, Declan T.
AU  - Curran, Tanya
AU  - Moore, Catherine
AU  - Cottrell, Simon
AU  - Kudasheva, Lyudmila
AU  - Djemileva, Sultan
AU  - Pleshkov, Boris
PY  - 2024
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11081882/
UR  - http://intor.torlakinstitut.com/handle/123456789/881
AB  - Background: The emergence of SARS‐CoV‐2 in late 2019 saw the implementation of public health and social measures (PHSM) by countries across Europe to reduce its transmission and impact on populations. Consequently, countries reported changes in influenza circulation and extensive disruptions to routine surveillance systems.MethodsWe describe the epidemiology of influenza in Europe between Weeks 40/2020 and 39/2022 compared to the 2016/17 to 2019/20 seasons, to assess the impact of the COVID‐19 pandemic and PHSM on surveillance systems and influenza circulation.ResultsLow detections of influenza were observed through primary care sentinel sources during seasonal influenza periods (Week 40 to 20); 56 (of 39,457 specimens tested; < 1% positivity) in 2020/21 and 7261 (of 64,153 specimens tested; 11% positivity) detections in 2021/22 were observed, compared to an average of 18,383 (of 50,544 specimens tested; 36% positivity) detections in 2016/17 to 2019/20. Similarly, 11 (of 19,989 specimens tested; < 1% positivity) and 1488 (of 23,636 specimens tested; 6% positivity) detections were reported through SARI surveillance sources in 2020/21 and 2021/22, respectively, compared to an average of 2850 (of 10,389 specimens tested; 27% positivity) detections in 2016/17 to 2019/20. However, the 2021/22 interseasonal period saw unusual increases in influenza detections across surveillance site types when PHSM were easing.ConclusionIn conclusion, findings suggest that the restriction and easing of PHSM measures were associated with variations in influenza detections. Our observations of out‐of‐season influenza activity highlight the importance of an integrated respiratory surveillance strategy to monitor circulating respiratory viruses throughout the year to inform optimal prevention and control strategies.
PB  - Wiley
T2  - Influenza and Other Respiratory Viruses
T1  - Impact of the COVID‐19 Pandemic on Influenza Circulation During the 2020/21 and 2021/22 Seasons, in Europe
IS  - 5
SP  - e13297
VL  - 18
DO  - 10.1111/irv.13297
ER  - 

@article{
author = "Sinnathamby, Mary A. and Meslé, Margaux M. I. and Mook, Piers and Pebody, Richard and Bino, Silvia and Hasibra, Iris and Bakunts, Nune and Abovyan, Romella and Khachtryan, Evgenia and Redlberger‐Fritz, Monika and Mursalova, Nazifa and Aliyeva, Firuza and Vysotskaya, Veronika and Shmialiova, Natallia and Karaban, Inna and Bossuyt, Nathalie and Barbezange, Cyril and Musa, Sanja and Vukmir, Nina Rodić and Ljubović, Amela Dedeić and Baštinac, Dijana and Vladimirov, Nadezhda and Korsun, Neli and Trifonova, Ivelina and Tabain, Irena and Petrović, Goranka and Karagiannis, Christos and Haralambous, Christos and Jirincova, Helena and Kyncl, Jan and Trebbien, Ramona and Vestergaard, Lasse Skafte and Sadikova, Olga and Eero, Irina and Metsoja, Eliisa and Ikonen, Niina and Lyytikäinen, Outi and Nohynek, Hanna and Fournier, Lucie and Guerrisi, Caroline and Valette, Martine and Machablishvili, Ani and Buda, Silke and Dürrwald, Ralf and Gioula, Georgia and Mary, Emmanouil and Mellou, Kassiani and Rózsa, Mónika and Molnár, Zsuzsanna and Armannsdottir, Brynja and Aspelund, Guðrún and O’Donnell, Joan and Domegan, Lisa and Connell, Jeff and Mandelboim, Michal and Glatman‐Freedman, Aharona and Puzelli, Simona and Palamara, Anna Teresa and Maraglino, Francesco and Smagulova, Smagulova Meiramgul Kanapiyanovna and Userbayev, Aidar Sharipkhanuly and Kalaveshi, Ariana and Jakupi, Xhevat and Gunga, Zana Kaçaniku and Otorbaeva, D. S and Abdyldaeva, S. Zh and Esengeldiev, G. M and Vasiļevska, Darja and Tomašūna, Kate Karolīna and Savicka, Oksana and Gargasiene, Greta and Muralyte, Svajune and Nguyen, Trung‐Nguyen and Mossong, Joel and Abdelrahman, Tamir T. and Melillo, Tanya and Zahra, Graziella and Melillo, Jackie and Rakocevic, Bozidarka and Zekovic, Zeljka and Medenica, Sanja and de Lange, Marit and Meijer, Adam and Kochinski, Dragan and Boshevska, Golubinka and Paulsen, Trinehessevik and Bragstad, Karoline and Brydak, Lidia B. and Hallmann, Ewelina and Szymański, Karol and Rodrigues, Ana Paula and Verdasca, Nuno and Guiomar, Raquel and Druc, Alina and Apostol, Mariana and Popescu, Rodica and Popovici, Odette and Lazar, Mihaela and Komissarov, Andrey B. and Fadeev, Artem and Stolyarov, Kirill and Protić, Jelena and Avdičová, Mária and Mečochová, Adriana and Staroňová, Edita and Sočan, Maja and Prosenc, Katarina and Berginc, Nataša and Larrauri, Amparo and Mazagatos, Clara and Pozo, Francisco and Appelqvist, Emma and Carnahan, AnnaSara and Cabecinhas, Ana Rita Goncalves and Spedaliero, Tania and Jafarov, Navaruz and Safarova, Tamanno and Barotova, Barno and Kovalchuk, Tatyana and Altas, Ayse Basak and Avci, Emine and Ozdemir, Betul and Ovliyakulova, Gurbangul and Dykhanovska, Tetiana and Demchyshyna, Iryna and Koshako, Oksana and Watson, Conall and Zambon, Maria and Lakhani, Anissa and Shepherd, Samantha and Bradley, Declan T. and Curran, Tanya and Moore, Catherine and Cottrell, Simon and Kudasheva, Lyudmila and Djemileva, Sultan and Pleshkov, Boris",
year = "2024",
abstract = "Background: The emergence of SARS‐CoV‐2 in late 2019 saw the implementation of public health and social measures (PHSM) by countries across Europe to reduce its transmission and impact on populations. Consequently, countries reported changes in influenza circulation and extensive disruptions to routine surveillance systems.MethodsWe describe the epidemiology of influenza in Europe between Weeks 40/2020 and 39/2022 compared to the 2016/17 to 2019/20 seasons, to assess the impact of the COVID‐19 pandemic and PHSM on surveillance systems and influenza circulation.ResultsLow detections of influenza were observed through primary care sentinel sources during seasonal influenza periods (Week 40 to 20); 56 (of 39,457 specimens tested; < 1% positivity) in 2020/21 and 7261 (of 64,153 specimens tested; 11% positivity) detections in 2021/22 were observed, compared to an average of 18,383 (of 50,544 specimens tested; 36% positivity) detections in 2016/17 to 2019/20. Similarly, 11 (of 19,989 specimens tested; < 1% positivity) and 1488 (of 23,636 specimens tested; 6% positivity) detections were reported through SARI surveillance sources in 2020/21 and 2021/22, respectively, compared to an average of 2850 (of 10,389 specimens tested; 27% positivity) detections in 2016/17 to 2019/20. However, the 2021/22 interseasonal period saw unusual increases in influenza detections across surveillance site types when PHSM were easing.ConclusionIn conclusion, findings suggest that the restriction and easing of PHSM measures were associated with variations in influenza detections. Our observations of out‐of‐season influenza activity highlight the importance of an integrated respiratory surveillance strategy to monitor circulating respiratory viruses throughout the year to inform optimal prevention and control strategies.",
publisher = "Wiley",
journal = "Influenza and Other Respiratory Viruses",
title = "Impact of the COVID‐19 Pandemic on Influenza Circulation During the 2020/21 and 2021/22 Seasons, in Europe",
number = "5",
pages = "e13297",
volume = "18",
doi = "10.1111/irv.13297"
}

Sinnathamby, Mary A., Meslé, Margaux M. I., Mook, P., Pebody, R., Bino, S., Hasibra, I., Bakunts, N., Abovyan, R., Khachtryan, E., Redlberger‐Fritz, M., Mursalova, N., Aliyeva, F., Vysotskaya, V., Shmialiova, N., Karaban, I., Bossuyt, N., Barbezange, C., Musa, S., Vukmir, N. R., Ljubović, A. D., Baštinac, D., Vladimirov, N., Korsun, N., Trifonova, I., Tabain, I., Petrović, G., Karagiannis, C., Haralambous, C., Jirincova, H., Kyncl, J., Trebbien, R., Vestergaard, L. S., Sadikova, O., Eero, I., Metsoja, E., Ikonen, N., Lyytikäinen, O., Nohynek, H., Fournier, L., Guerrisi, C., Valette, M., Machablishvili, A., Buda, S., Dürrwald, R., Gioula, G., Mary, E., Mellou, K., Rózsa, M., Molnár, Z., Armannsdottir, B., Aspelund, G., O’Donnell, J., Domegan, L., Connell, J., Mandelboim, M., Glatman‐Freedman, A., Puzelli, S., Palamara, A. T., Maraglino, F., Smagulova, S. M. K., Userbayev, A. S., Kalaveshi, A., Jakupi, X., Gunga, Z. K., Otorbaeva, D. S., Abdyldaeva, S. Z., Esengeldiev, G. M., Vasiļevska, D., Tomašūna, K. K., Savicka, O., Gargasiene, G., Muralyte, S., Nguyen, T., Mossong, J., Abdelrahman, T. T., Melillo, T., Zahra, G., Melillo, J., Rakocevic, B., Zekovic, Z., Medenica, S., de Lange, M., Meijer, A., Kochinski, D., Boshevska, G., Paulsen, T., Bragstad, K., Brydak, L. B., Hallmann, E., Szymański, K., Rodrigues, A. P., Verdasca, N., Guiomar, R., Druc, A., Apostol, M., Popescu, R., Popovici, O., Lazar, M., Komissarov, A. B., Fadeev, A., Stolyarov, K., Protić, J., Avdičová, M., Mečochová, A., Staroňová, E., Sočan, M., Prosenc, K., Berginc, N., Larrauri, A., Mazagatos, C., Pozo, F., Appelqvist, E., Carnahan, A., Cabecinhas, A. R. G., Spedaliero, T., Jafarov, N., Safarova, T., Barotova, B., Kovalchuk, T., Altas, A. B., Avci, E., Ozdemir, B., Ovliyakulova, G., Dykhanovska, T., Demchyshyna, I., Koshako, O., Watson, C., Zambon, M., Lakhani, A., Shepherd, S., Bradley, D. T., Curran, T., Moore, C., Cottrell, S., Kudasheva, L., Djemileva, S.,& Pleshkov, B.. (2024). Impact of the COVID‐19 Pandemic on Influenza Circulation During the 2020/21 and 2021/22 Seasons, in Europe. in Influenza and Other Respiratory Viruses
Wiley., 18(5), e13297.
https://doi.org/10.1111/irv.13297

Sinnathamby M, Meslé M, Mook P, Pebody R, Bino S, Hasibra I, Bakunts N, Abovyan R, Khachtryan E, Redlberger‐Fritz M, Mursalova N, Aliyeva F, Vysotskaya V, Shmialiova N, Karaban I, Bossuyt N, Barbezange C, Musa S, Vukmir NR, Ljubović AD, Baštinac D, Vladimirov N, Korsun N, Trifonova I, Tabain I, Petrović G, Karagiannis C, Haralambous C, Jirincova H, Kyncl J, Trebbien R, Vestergaard LS, Sadikova O, Eero I, Metsoja E, Ikonen N, Lyytikäinen O, Nohynek H, Fournier L, Guerrisi C, Valette M, Machablishvili A, Buda S, Dürrwald R, Gioula G, Mary E, Mellou K, Rózsa M, Molnár Z, Armannsdottir B, Aspelund G, O’Donnell J, Domegan L, Connell J, Mandelboim M, Glatman‐Freedman A, Puzelli S, Palamara AT, Maraglino F, Smagulova SMK, Userbayev AS, Kalaveshi A, Jakupi X, Gunga ZK, Otorbaeva DS, Abdyldaeva SZ, Esengeldiev GM, Vasiļevska D, Tomašūna KK, Savicka O, Gargasiene G, Muralyte S, Nguyen T, Mossong J, Abdelrahman TT, Melillo T, Zahra G, Melillo J, Rakocevic B, Zekovic Z, Medenica S, de Lange M, Meijer A, Kochinski D, Boshevska G, Paulsen T, Bragstad K, Brydak LB, Hallmann E, Szymański K, Rodrigues AP, Verdasca N, Guiomar R, Druc A, Apostol M, Popescu R, Popovici O, Lazar M, Komissarov AB, Fadeev A, Stolyarov K, Protić J, Avdičová M, Mečochová A, Staroňová E, Sočan M, Prosenc K, Berginc N, Larrauri A, Mazagatos C, Pozo F, Appelqvist E, Carnahan A, Cabecinhas ARG, Spedaliero T, Jafarov N, Safarova T, Barotova B, Kovalchuk T, Altas AB, Avci E, Ozdemir B, Ovliyakulova G, Dykhanovska T, Demchyshyna I, Koshako O, Watson C, Zambon M, Lakhani A, Shepherd S, Bradley DT, Curran T, Moore C, Cottrell S, Kudasheva L, Djemileva S, Pleshkov B. Impact of the COVID‐19 Pandemic on Influenza Circulation During the 2020/21 and 2021/22 Seasons, in Europe. in Influenza and Other Respiratory Viruses. 2024;18(5):e13297.
doi:10.1111/irv.13297 .

Sinnathamby, Mary A., Meslé, Margaux M. I., Mook, Piers, Pebody, Richard, Bino, Silvia, Hasibra, Iris, Bakunts, Nune, Abovyan, Romella, Khachtryan, Evgenia, Redlberger‐Fritz, Monika, Mursalova, Nazifa, Aliyeva, Firuza, Vysotskaya, Veronika, Shmialiova, Natallia, Karaban, Inna, Bossuyt, Nathalie, Barbezange, Cyril, Musa, Sanja, Vukmir, Nina Rodić, Ljubović, Amela Dedeić, Baštinac, Dijana, Vladimirov, Nadezhda, Korsun, Neli, Trifonova, Ivelina, Tabain, Irena, Petrović, Goranka, Karagiannis, Christos, Haralambous, Christos, Jirincova, Helena, Kyncl, Jan, Trebbien, Ramona, Vestergaard, Lasse Skafte, Sadikova, Olga, Eero, Irina, Metsoja, Eliisa, Ikonen, Niina, Lyytikäinen, Outi, Nohynek, Hanna, Fournier, Lucie, Guerrisi, Caroline, Valette, Martine, Machablishvili, Ani, Buda, Silke, Dürrwald, Ralf, Gioula, Georgia, Mary, Emmanouil, Mellou, Kassiani, Rózsa, Mónika, Molnár, Zsuzsanna, Armannsdottir, Brynja, Aspelund, Guðrún, O’Donnell, Joan, Domegan, Lisa, Connell, Jeff, Mandelboim, Michal, Glatman‐Freedman, Aharona, Puzelli, Simona, Palamara, Anna Teresa, Maraglino, Francesco, Smagulova, Smagulova Meiramgul Kanapiyanovna, Userbayev, Aidar Sharipkhanuly, Kalaveshi, Ariana, Jakupi, Xhevat, Gunga, Zana Kaçaniku, Otorbaeva, D. S, Abdyldaeva, S. Zh, Esengeldiev, G. M, Vasiļevska, Darja, Tomašūna, Kate Karolīna, Savicka, Oksana, Gargasiene, Greta, Muralyte, Svajune, Nguyen, Trung‐Nguyen, Mossong, Joel, Abdelrahman, Tamir T., Melillo, Tanya, Zahra, Graziella, Melillo, Jackie, Rakocevic, Bozidarka, Zekovic, Zeljka, Medenica, Sanja, de Lange, Marit, Meijer, Adam, Kochinski, Dragan, Boshevska, Golubinka, Paulsen, Trinehessevik, Bragstad, Karoline, Brydak, Lidia B., Hallmann, Ewelina, Szymański, Karol, Rodrigues, Ana Paula, Verdasca, Nuno, Guiomar, Raquel, Druc, Alina, Apostol, Mariana, Popescu, Rodica, Popovici, Odette, Lazar, Mihaela, Komissarov, Andrey B., Fadeev, Artem, Stolyarov, Kirill, Protić, Jelena, Avdičová, Mária, Mečochová, Adriana, Staroňová, Edita, Sočan, Maja, Prosenc, Katarina, Berginc, Nataša, Larrauri, Amparo, Mazagatos, Clara, Pozo, Francisco, Appelqvist, Emma, Carnahan, AnnaSara, Cabecinhas, Ana Rita Goncalves, Spedaliero, Tania, Jafarov, Navaruz, Safarova, Tamanno, Barotova, Barno, Kovalchuk, Tatyana, Altas, Ayse Basak, Avci, Emine, Ozdemir, Betul, Ovliyakulova, Gurbangul, Dykhanovska, Tetiana, Demchyshyna, Iryna, Koshako, Oksana, Watson, Conall, Zambon, Maria, Lakhani, Anissa, Shepherd, Samantha, Bradley, Declan T., Curran, Tanya, Moore, Catherine, Cottrell, Simon, Kudasheva, Lyudmila, Djemileva, Sultan, Pleshkov, Boris, "Impact of the COVID‐19 Pandemic on Influenza Circulation During the 2020/21 and 2021/22 Seasons, in Europe" in Influenza and Other Respiratory Viruses, 18, no. 5 (2024):e13297,
https://doi.org/10.1111/irv.13297 . .

TY  - JOUR
AU  - Miljković, Radmila
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Kovačević, Ana
AU  - Lopandić, Zorana
AU  - Popović, Mina
AU  - Gavrović-Jankulović, Marija
AU  - Schabussova, Irma
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/886
AB  - Compared to the general population, patients with inflammatory bowel disease (IBD) are less likely to be vaccinated, putting them at an increased risk of vaccine-preventable illnesses. This risk is further compounded by the immunosuppressive therapies commonly used in IBD management. Therefore, developing new treatments for IBD that maintain immune function is crucial, as successful management can lead to better vaccination outcomes and overall health for these patients. Here, we investigate the potential of recombinant banana lectin (rBanLec) as a supporting therapeutic measure to improve IBD control and possibly increase vaccination rates among IBD patients. By examining the therapeutic efficacy of rBanLec in a murine model of experimental colitis, we aim to lay the foundation for its application in improving vaccination outcomes. After inducing experimental colitis in C57BL/6 and BALB/c mice with 2,4,6-trinitrobenzene sulfonic acid, we treated animals orally with varying doses of rBanLec 0.1–10 µg/mL (0.01—1 µg/dose) during the course of the disease. We assessed the severity of colitis and rBanLec’s modulation of the immune response compared to control groups. rBanLec administration resulted in an inverse dose–response reduction in colitis severity (less pronounced weight loss, less shortening of the colon) and an improved recovery profile, highlighting its therapeutic potential. Notably, rBanLec-treated mice exhibited significant modulation of the immune response, favoring anti-inflammatory pathways (primarily reduction in a local [TNFα]/[IL-10]) crucial for effective vaccination. Our findings suggest that rBanLec could mitigate the adverse effects of immunosuppressive therapy on vaccine responsiveness in IBD patients. By improving the underlying immune response, rBanLec may increase the efficacy of vaccinations, offering a dual benefit of disease management and prevention of vaccine-preventable illnesses. Further studies are required to translate these findings into clinical practice.
PB  - MDPI
T2  - Nutrients
T2  - Nutrients
T1  - Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease
IS  - 11
SP  - 1705
VL  - 16
DO  - 10.3390/nu16111705
ER  - 

@article{
author = "Miljković, Radmila and Marinković, Emilija and Lukić, Ivana and Kovačević, Ana and Lopandić, Zorana and Popović, Mina and Gavrović-Jankulović, Marija and Schabussova, Irma and Inić-Kanada, Aleksandra and Stojanović, Marijana",
year = "2024",
abstract = "Compared to the general population, patients with inflammatory bowel disease (IBD) are less likely to be vaccinated, putting them at an increased risk of vaccine-preventable illnesses. This risk is further compounded by the immunosuppressive therapies commonly used in IBD management. Therefore, developing new treatments for IBD that maintain immune function is crucial, as successful management can lead to better vaccination outcomes and overall health for these patients. Here, we investigate the potential of recombinant banana lectin (rBanLec) as a supporting therapeutic measure to improve IBD control and possibly increase vaccination rates among IBD patients. By examining the therapeutic efficacy of rBanLec in a murine model of experimental colitis, we aim to lay the foundation for its application in improving vaccination outcomes. After inducing experimental colitis in C57BL/6 and BALB/c mice with 2,4,6-trinitrobenzene sulfonic acid, we treated animals orally with varying doses of rBanLec 0.1–10 µg/mL (0.01—1 µg/dose) during the course of the disease. We assessed the severity of colitis and rBanLec’s modulation of the immune response compared to control groups. rBanLec administration resulted in an inverse dose–response reduction in colitis severity (less pronounced weight loss, less shortening of the colon) and an improved recovery profile, highlighting its therapeutic potential. Notably, rBanLec-treated mice exhibited significant modulation of the immune response, favoring anti-inflammatory pathways (primarily reduction in a local [TNFα]/[IL-10]) crucial for effective vaccination. Our findings suggest that rBanLec could mitigate the adverse effects of immunosuppressive therapy on vaccine responsiveness in IBD patients. By improving the underlying immune response, rBanLec may increase the efficacy of vaccinations, offering a dual benefit of disease management and prevention of vaccine-preventable illnesses. Further studies are required to translate these findings into clinical practice.",
publisher = "MDPI",
journal = "Nutrients, Nutrients",
title = "Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease",
number = "11",
pages = "1705",
volume = "16",
doi = "10.3390/nu16111705"
}

Miljković, R., Marinković, E., Lukić, I., Kovačević, A., Lopandić, Z., Popović, M., Gavrović-Jankulović, M., Schabussova, I., Inić-Kanada, A.,& Stojanović, M.. (2024). Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease. in Nutrients
MDPI., 16(11), 1705.
https://doi.org/10.3390/nu16111705

Miljković R, Marinković E, Lukić I, Kovačević A, Lopandić Z, Popović M, Gavrović-Jankulović M, Schabussova I, Inić-Kanada A, Stojanović M. Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease. in Nutrients. 2024;16(11):1705.
doi:10.3390/nu16111705 .

Miljković, Radmila, Marinković, Emilija, Lukić, Ivana, Kovačević, Ana, Lopandić, Zorana, Popović, Mina, Gavrović-Jankulović, Marija, Schabussova, Irma, Inić-Kanada, Aleksandra, Stojanović, Marijana, "Banana Lectin: A Novel Immunomodulatory Strategy for Mitigating Inflammatory Bowel Disease" in Nutrients, 16, no. 11 (2024):1705,
https://doi.org/10.3390/nu16111705 . .

TY  - DATA
AU  - Patiño-Guillén, Gerardo
AU  - Pešović, Jovan
AU  - Panić, Marko
AU  - Savić-Pavićević, Dušanka
AU  - Bošković, Filip
AU  - Keyser, Ulrich Felix
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/866
AB  - This PDF file includes: → Supplementary Figures 1 to 29 → Supplementary Tables 1 to 8
PB  - Nature
T2  - Nature Communications
T1  - Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.
IS  - 1
VL  - 15
DO  - 10.17863/CAM.104528
ER  - 

@misc{
author = "Patiño-Guillén, Gerardo and Pešović, Jovan and Panić, Marko and Savić-Pavićević, Dušanka and Bošković, Filip and Keyser, Ulrich Felix",
year = "2024",
abstract = "This PDF file includes: → Supplementary Figures 1 to 29 → Supplementary Tables 1 to 8",
publisher = "Nature",
journal = "Nature Communications",
title = "Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.",
number = "1",
volume = "15",
doi = "10.17863/CAM.104528"
}

Patiño-Guillén, G., Pešović, J., Panić, M., Savić-Pavićević, D., Bošković, F.,& Keyser, U. F.. (2024). Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.. in Nature Communications
Nature., 15(1).
https://doi.org/10.17863/CAM.104528

Patiño-Guillén G, Pešović J, Panić M, Savić-Pavićević D, Bošković F, Keyser UF. Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.. in Nature Communications. 2024;15(1).
doi:10.17863/CAM.104528 .

Patiño-Guillén, Gerardo, Pešović, Jovan, Panić, Marko, Savić-Pavićević, Dušanka, Bošković, Filip, Keyser, Ulrich Felix, "Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8." in Nature Communications, 15, no. 1 (2024),
https://doi.org/10.17863/CAM.104528 . .

TY  - CONF
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Pavić, Aleksandar
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/885
AB  - Antimicrobial resistance (AMR) arises whenbacteria and other microbes stop respondingto medications. AMR is now recognized as oneof serious global health threats, repeatedlyappearing in the World Health Organization’s(WHO) lists of urgent global health challenges,including the 2024 list. It is taking a fatal toll– nearly 5 million deaths globally per year areassociated with AMR, encompassing 1.27 milliondirectly attributed to AMR. The COVID-19pandemic paved the way for aggravation ofbacterial AMR – primarily due to enhancementin unspecific and unjustified prescription anduse of broad-spectrum antibiotics, resulting inwhat is now recognized as „silent pandemic ofAMR“. Bacteriophages (phages) are natural andspecific predators of bacteria - viruses that caninfect, replicate inside and lyse arguably anybacteria. Their therapeutic potential is beinghastily evaluated through different approaches:in silico, in vitro, ex vivo and in vivo – in laboratoryanimals as well as in human case and clinicalstudies. Although the results are promising,bacteria rapidly develop resistance againstphages, which why the isolation and researchof new phages is needed. Our work is concentratedon three bacterial species for which criticalpriority by WHO has been declared – carbapenem-resistant Acinetobacter baumannii,Pseudomonas aeruginosa and Klebsiella pneumoniae.Twenty distinct pathogenic strains ofA. baumannii, 6 K. pneumoniae and 6 P. aeruginosawere used as targets for bacteriophageisolation, and total of 14, 22 and 8 potentiallydistinct phages were collected, respectively. Allstrains were nosocomial isolates obtained fromvarious tissues, including from terminally ill patients.Six phages were characterized in detail.In particular, phage vB_AbaM_ISTD was appliedagainst A. baumannii in zebrafish embryomodel of systemic infection, and demonstratedpowerful therapeutic potential, eradicating theinfection. Interestingly, its DNA was characterizedwith highly modified thymidine (amassing1228 Da), making it the largest non-canonicaldeoxynucleoside reported so far.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - Bacteriophages of multidrug-resistant nosocomial pathogens – Belgrade experience
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_intor_885
ER  - 

@conference{
author = "Vukotić, Goran and Obradović, Mina and Plačkić, Nikola and Kljajević, Nemanja and Pavić, Aleksandar and Kekić, Dušan and Gajić, Ina and Kojić, Milan and Stanisavljević, Nemanja",
year = "2024",
abstract = "Antimicrobial resistance (AMR) arises whenbacteria and other microbes stop respondingto medications. AMR is now recognized as oneof serious global health threats, repeatedlyappearing in the World Health Organization’s(WHO) lists of urgent global health challenges,including the 2024 list. It is taking a fatal toll– nearly 5 million deaths globally per year areassociated with AMR, encompassing 1.27 milliondirectly attributed to AMR. The COVID-19pandemic paved the way for aggravation ofbacterial AMR – primarily due to enhancementin unspecific and unjustified prescription anduse of broad-spectrum antibiotics, resulting inwhat is now recognized as „silent pandemic ofAMR“. Bacteriophages (phages) are natural andspecific predators of bacteria - viruses that caninfect, replicate inside and lyse arguably anybacteria. Their therapeutic potential is beinghastily evaluated through different approaches:in silico, in vitro, ex vivo and in vivo – in laboratoryanimals as well as in human case and clinicalstudies. Although the results are promising,bacteria rapidly develop resistance againstphages, which why the isolation and researchof new phages is needed. Our work is concentratedon three bacterial species for which criticalpriority by WHO has been declared – carbapenem-resistant Acinetobacter baumannii,Pseudomonas aeruginosa and Klebsiella pneumoniae.Twenty distinct pathogenic strains ofA. baumannii, 6 K. pneumoniae and 6 P. aeruginosawere used as targets for bacteriophageisolation, and total of 14, 22 and 8 potentiallydistinct phages were collected, respectively. Allstrains were nosocomial isolates obtained fromvarious tissues, including from terminally ill patients.Six phages were characterized in detail.In particular, phage vB_AbaM_ISTD was appliedagainst A. baumannii in zebrafish embryomodel of systemic infection, and demonstratedpowerful therapeutic potential, eradicating theinfection. Interestingly, its DNA was characterizedwith highly modified thymidine (amassing1228 Da), making it the largest non-canonicaldeoxynucleoside reported so far.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "Bacteriophages of multidrug-resistant nosocomial pathogens – Belgrade experience",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_intor_885"
}

Vukotić, G., Obradović, M., Plačkić, N., Kljajević, N., Pavić, A., Kekić, D., Gajić, I., Kojić, M.,& Stanisavljević, N.. (2024). Bacteriophages of multidrug-resistant nosocomial pathogens – Belgrade experience. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 121-121.
https://hdl.handle.net/21.15107/rcub_intor_885

Vukotić G, Obradović M, Plačkić N, Kljajević N, Pavić A, Kekić D, Gajić I, Kojić M, Stanisavljević N. Bacteriophages of multidrug-resistant nosocomial pathogens – Belgrade experience. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:121-121.
https://hdl.handle.net/21.15107/rcub_intor_885 .

Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, Kljajević, Nemanja, Pavić, Aleksandar, Kekić, Dušan, Gajić, Ina, Kojić, Milan, Stanisavljević, Nemanja, "Bacteriophages of multidrug-resistant nosocomial pathogens – Belgrade experience" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):121-121,
https://hdl.handle.net/21.15107/rcub_intor_885 .

TY  - JOUR
AU  - Đurašinović, Tatjana
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Ravnsborg, Tina
AU  - Blagojević, Gordan
AU  - Burazer, Lidija
AU  - Jensen, Ole N.
AU  - Gavrović-Jankulović, Marija
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/882
AB  - Food allergies mediated by specific IgE (sIgE) have a significant socioeconomic impact on society. Evaluating the IgE cross-reactivity between allergens from different allergen sources can enable the better management of these potentially life-threatening adverse reactions to food proteins and enhance food safety. A novel banana fruit allergen, S-adenosyl-L-homocysteine hydrolase (SAHH), has been recently identified and its recombinant homolog was heterologously overproduced in E. coli. In this study, we performed a search in the NCBI (National Center for Biotechnology Information) for SAHH homologs in ryegrass, latex, and kiwifruit, all of which are commonly associated with pollen-latex-fruit syndrome. In addition, Western immunoblot analysis was utilized to identify the cross-reactive IgE to banana SAHH in the sera of patients with a latex allergy, kiwifruit allergy, and ryegrass allergy. ClustalOmega analysis showed more than 92% amino acid sequence identity among the banana SAHH homologs in ryegrass, latex, and kiwifruit. In addition to five B-cell epitopes, in silico analysis predicted eleven T-cell epitopes in banana SAHH, seventeen in kiwifruit SAHH, twelve in ryegrass SAHH, and eight in latex SAHH, which were related to the seven-allele HLA reference set (HLA-DRB1*03:01, HLA-DRB1*07:01, HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DRB3*02:02, HLA-DRB4*01:01, HLA-DRB5*01:01). Four T-cell epitopes were identical in banana and kiwifruit SAHH (positions 328, 278, 142, 341), as well as banana and ryegrass SAHH (positions 278, 142, 96, and 341). All four SAHHs shared two T-cell epitopes (positions 278 and 341). In line with the high amino acid sequence identity and B-cell epitope homology among the analyzed proteins, the cross-reactive IgE to banana SAHH was detected in three of three latex-allergic patients, five of six ryegrass-allergic patients, and two of three kiwifruit-allergic patients. Although banana SAHH has only been studied in a small group of allergic individuals, it is a novel cross-reactive food allergen that should be considered when testing for pollen-latex-fruit syndrome.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals
IS  - 11
SP  - 5800
VL  - 25
DO  - 10.3390/ijms25115800
ER  - 

@article{
author = "Đurašinović, Tatjana and Lopandić, Zorana and Protić-Rosić, Isidora and Ravnsborg, Tina and Blagojević, Gordan and Burazer, Lidija and Jensen, Ole N. and Gavrović-Jankulović, Marija",
year = "2024",
abstract = "Food allergies mediated by specific IgE (sIgE) have a significant socioeconomic impact on society. Evaluating the IgE cross-reactivity between allergens from different allergen sources can enable the better management of these potentially life-threatening adverse reactions to food proteins and enhance food safety. A novel banana fruit allergen, S-adenosyl-L-homocysteine hydrolase (SAHH), has been recently identified and its recombinant homolog was heterologously overproduced in E. coli. In this study, we performed a search in the NCBI (National Center for Biotechnology Information) for SAHH homologs in ryegrass, latex, and kiwifruit, all of which are commonly associated with pollen-latex-fruit syndrome. In addition, Western immunoblot analysis was utilized to identify the cross-reactive IgE to banana SAHH in the sera of patients with a latex allergy, kiwifruit allergy, and ryegrass allergy. ClustalOmega analysis showed more than 92% amino acid sequence identity among the banana SAHH homologs in ryegrass, latex, and kiwifruit. In addition to five B-cell epitopes, in silico analysis predicted eleven T-cell epitopes in banana SAHH, seventeen in kiwifruit SAHH, twelve in ryegrass SAHH, and eight in latex SAHH, which were related to the seven-allele HLA reference set (HLA-DRB1*03:01, HLA-DRB1*07:01, HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DRB3*02:02, HLA-DRB4*01:01, HLA-DRB5*01:01). Four T-cell epitopes were identical in banana and kiwifruit SAHH (positions 328, 278, 142, 341), as well as banana and ryegrass SAHH (positions 278, 142, 96, and 341). All four SAHHs shared two T-cell epitopes (positions 278 and 341). In line with the high amino acid sequence identity and B-cell epitope homology among the analyzed proteins, the cross-reactive IgE to banana SAHH was detected in three of three latex-allergic patients, five of six ryegrass-allergic patients, and two of three kiwifruit-allergic patients. Although banana SAHH has only been studied in a small group of allergic individuals, it is a novel cross-reactive food allergen that should be considered when testing for pollen-latex-fruit syndrome.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals",
number = "11",
pages = "5800",
volume = "25",
doi = "10.3390/ijms25115800"
}

Đurašinović, T., Lopandić, Z., Protić-Rosić, I., Ravnsborg, T., Blagojević, G., Burazer, L., Jensen, O. N.,& Gavrović-Jankulović, M.. (2024). Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. in International Journal of Molecular Sciences
MDPI., 25(11), 5800.
https://doi.org/10.3390/ijms25115800

Đurašinović T, Lopandić Z, Protić-Rosić I, Ravnsborg T, Blagojević G, Burazer L, Jensen ON, Gavrović-Jankulović M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. in International Journal of Molecular Sciences. 2024;25(11):5800.
doi:10.3390/ijms25115800 .

Đurašinović, Tatjana, Lopandić, Zorana, Protić-Rosić, Isidora, Ravnsborg, Tina, Blagojević, Gordan, Burazer, Lidija, Jensen, Ole N., Gavrović-Jankulović, Marija, "Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals" in International Journal of Molecular Sciences, 25, no. 11 (2024):5800,
https://doi.org/10.3390/ijms25115800 . .

TY  - DATA
AU  - Đurašinović, Tatjana
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Ravnsborg, Tina
AU  - Blagojević, Gordan
AU  - Burazer, Lidija
AU  - Jensen, Ole N.
AU  - Gavrović-Jankulović, Marija
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/883
AB  - Supplementary Table S2: A)List of T-cell epitopes in kiwifruit SAHH. B)List of T cell epitopes in latex SAHH.C)List of T-cell epitopes in ryegrass SAHH
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary information for the article: Đurašinović, T.; Lopandić, Z.; Protić-Rosić, I.; Ravnsborg, T.; Blagojević, G.; Burazer, L.; Jensen, O. N.; Gavrović-Jankulović, M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. International Journal of Molecular Sciences 2024, 25 (11), 5800. https://doi.org/10.3390/ijms25115800.
UR  - https://hdl.handle.net/21.15107/rcub_intor_883
ER  - 

@misc{
author = "Đurašinović, Tatjana and Lopandić, Zorana and Protić-Rosić, Isidora and Ravnsborg, Tina and Blagojević, Gordan and Burazer, Lidija and Jensen, Ole N. and Gavrović-Jankulović, Marija",
year = "2024",
abstract = "Supplementary Table S2: A)List of T-cell epitopes in kiwifruit SAHH. B)List of T cell epitopes in latex SAHH.C)List of T-cell epitopes in ryegrass SAHH",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary information for the article: Đurašinović, T.; Lopandić, Z.; Protić-Rosić, I.; Ravnsborg, T.; Blagojević, G.; Burazer, L.; Jensen, O. N.; Gavrović-Jankulović, M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. International Journal of Molecular Sciences 2024, 25 (11), 5800. https://doi.org/10.3390/ijms25115800.",
url = "https://hdl.handle.net/21.15107/rcub_intor_883"
}

Đurašinović, T., Lopandić, Z., Protić-Rosić, I., Ravnsborg, T., Blagojević, G., Burazer, L., Jensen, O. N.,& Gavrović-Jankulović, M.. (2024). Supplementary information for the article: Đurašinović, T.; Lopandić, Z.; Protić-Rosić, I.; Ravnsborg, T.; Blagojević, G.; Burazer, L.; Jensen, O. N.; Gavrović-Jankulović, M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. International Journal of Molecular Sciences 2024, 25 (11), 5800. https://doi.org/10.3390/ijms25115800.. in International Journal of Molecular Sciences
MDPI..
https://hdl.handle.net/21.15107/rcub_intor_883

Đurašinović T, Lopandić Z, Protić-Rosić I, Ravnsborg T, Blagojević G, Burazer L, Jensen ON, Gavrović-Jankulović M. Supplementary information for the article: Đurašinović, T.; Lopandić, Z.; Protić-Rosić, I.; Ravnsborg, T.; Blagojević, G.; Burazer, L.; Jensen, O. N.; Gavrović-Jankulović, M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. International Journal of Molecular Sciences 2024, 25 (11), 5800. https://doi.org/10.3390/ijms25115800.. in International Journal of Molecular Sciences. 2024;.
https://hdl.handle.net/21.15107/rcub_intor_883 .

Đurašinović, Tatjana, Lopandić, Zorana, Protić-Rosić, Isidora, Ravnsborg, Tina, Blagojević, Gordan, Burazer, Lidija, Jensen, Ole N., Gavrović-Jankulović, Marija, "Supplementary information for the article: Đurašinović, T.; Lopandić, Z.; Protić-Rosić, I.; Ravnsborg, T.; Blagojević, G.; Burazer, L.; Jensen, O. N.; Gavrović-Jankulović, M. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals. International Journal of Molecular Sciences 2024, 25 (11), 5800. https://doi.org/10.3390/ijms25115800." in International Journal of Molecular Sciences (2024),
https://hdl.handle.net/21.15107/rcub_intor_883 .

TY  - JOUR
AU  - Pavlović, Marija
AU  - Margetić, Aleksandra
AU  - Leonardi, Adrijana
AU  - Križaj, Igor
AU  - Kojić, Milan
AU  - Vujčić, Zoran
AU  - Šokarda Slavić, Marinela
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/863
AB  - This study focuses on the isolation, purification, and characterisation of endo-polygalacturonase II from Aspergillus tubingensis FAT43, particularly emphasising its potential applications in the fruit juice industry. A comprehensive screening test revealed the temporal dynamics of endo-polygalacturonase production during a 96-hour fermentation process. The purification process, involving ammonium sulfate and ethanol precipitation followed by ion-exchange chromatography, resulted in a 3.3-fold purification of PG II with a yield of 16% and a specific activity of 6001.67 U mg−1. Molecular analysis confirmed the identity of PG II, its gene (pgaII), and a high degree of sequence identity with Aspergillus tubingensis in the SWISS-PROT database. The optimal pH for PG II activity was 3.5–4.5, with robust stability across a broad pH spectrum (3–7). The enzyme exhibited optimal temperature activity at 45 °C, with a retention of 90% activity at 50 °C. The calculated activation energy for PG II was 62.1 kJ mol−1, indicating good stability. Inactivation kinetics revealed a half-life of 13.7 h at 40 °C, 5.4 h at 50 °C, and 0.85 h at 60 °C, with an activation energy of denaturation of 32.8 kJ mol−1. Compared to literature-reported PGs, PG II from A. tubingensis FAT43 demonstrated superior thermal stability. Hydrolysis experiments on different pectins revealed the highest specificity for non-methylated substrates (polygalacturonic acid). In fruit juice processing, PG II significantly increased juice yield and clarity, with the highest impact observed in strawberry juice. Antioxidant activity assays indicated enhanced antioxidant potential in enzyme-treated juices, especially strawberry, quince, and apple juices. The study highlights PG II's potential as an industrially valuable enzyme for fruit juice processing, offering improved thermostability and versatility across various fruit types.
PB  - Royal Society of Chemistry
T2  - Food & Function
T1  - Improvement of fruit juice quality: novel endo-polygalacturonase II from Aspergillus tubingensis FAT 43 for enhanced liquefaction, clarification, and antioxidant potential
DO  - 10.1039/D3FO05297D
ER  - 

@article{
author = "Pavlović, Marija and Margetić, Aleksandra and Leonardi, Adrijana and Križaj, Igor and Kojić, Milan and Vujčić, Zoran and Šokarda Slavić, Marinela",
year = "2024",
abstract = "This study focuses on the isolation, purification, and characterisation of endo-polygalacturonase II from Aspergillus tubingensis FAT43, particularly emphasising its potential applications in the fruit juice industry. A comprehensive screening test revealed the temporal dynamics of endo-polygalacturonase production during a 96-hour fermentation process. The purification process, involving ammonium sulfate and ethanol precipitation followed by ion-exchange chromatography, resulted in a 3.3-fold purification of PG II with a yield of 16% and a specific activity of 6001.67 U mg−1. Molecular analysis confirmed the identity of PG II, its gene (pgaII), and a high degree of sequence identity with Aspergillus tubingensis in the SWISS-PROT database. The optimal pH for PG II activity was 3.5–4.5, with robust stability across a broad pH spectrum (3–7). The enzyme exhibited optimal temperature activity at 45 °C, with a retention of 90% activity at 50 °C. The calculated activation energy for PG II was 62.1 kJ mol−1, indicating good stability. Inactivation kinetics revealed a half-life of 13.7 h at 40 °C, 5.4 h at 50 °C, and 0.85 h at 60 °C, with an activation energy of denaturation of 32.8 kJ mol−1. Compared to literature-reported PGs, PG II from A. tubingensis FAT43 demonstrated superior thermal stability. Hydrolysis experiments on different pectins revealed the highest specificity for non-methylated substrates (polygalacturonic acid). In fruit juice processing, PG II significantly increased juice yield and clarity, with the highest impact observed in strawberry juice. Antioxidant activity assays indicated enhanced antioxidant potential in enzyme-treated juices, especially strawberry, quince, and apple juices. The study highlights PG II's potential as an industrially valuable enzyme for fruit juice processing, offering improved thermostability and versatility across various fruit types.",
publisher = "Royal Society of Chemistry",
journal = "Food & Function",
title = "Improvement of fruit juice quality: novel endo-polygalacturonase II from Aspergillus tubingensis FAT 43 for enhanced liquefaction, clarification, and antioxidant potential",
doi = "10.1039/D3FO05297D"
}

Pavlović, M., Margetić, A., Leonardi, A., Križaj, I., Kojić, M., Vujčić, Z.,& Šokarda Slavić, M.. (2024). Improvement of fruit juice quality: novel endo-polygalacturonase II from Aspergillus tubingensis FAT 43 for enhanced liquefaction, clarification, and antioxidant potential. in Food & Function
Royal Society of Chemistry..
https://doi.org/10.1039/D3FO05297D

Pavlović M, Margetić A, Leonardi A, Križaj I, Kojić M, Vujčić Z, Šokarda Slavić M. Improvement of fruit juice quality: novel endo-polygalacturonase II from Aspergillus tubingensis FAT 43 for enhanced liquefaction, clarification, and antioxidant potential. in Food & Function. 2024;.
doi:10.1039/D3FO05297D .

Pavlović, Marija, Margetić, Aleksandra, Leonardi, Adrijana, Križaj, Igor, Kojić, Milan, Vujčić, Zoran, Šokarda Slavić, Marinela, "Improvement of fruit juice quality: novel endo-polygalacturonase II from Aspergillus tubingensis FAT 43 for enhanced liquefaction, clarification, and antioxidant potential" in Food & Function (2024),
https://doi.org/10.1039/D3FO05297D . .

TY  - CONF
AU  - Panić, Marko
AU  - Prijić, Ivana
AU  - Simić, Mihajlo
AU  - Ćuruvija, Ivana
AU  - Lukić, Ivana
AU  - Drgačević, Luka
AU  - Kojić, Milan
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/880
AB  - Diphtheria and tetanus, once formidable causes of morbidity and mortality worldwide, have seen their threats markedly diminished through the advent and widespread use of vaccines. This review article delves into the historical journey of diphtheria and tetanus vaccines, evaluates their current status in global immunization programs, and explores future perspectives in their evolution and implementation. The inception of diphtheria and tetanus vaccines marked a pivotal shift in infectious disease control. The development of diphtheria toxoid by Emil von Behring in the late 19th century and the subsequent creation of tetanus toxoid in the early 20th century set the stage for large-scale immunization efforts. These efforts were bolstered in the mid-20th century with the integration of these toxoids into combination vaccines, notably the DTP (diphtheria-tetanus-pertussis) vaccine, facilitating broader immunization coverage and enhanced public health outcomes. Currently, the inclusion of diphtheria and tetanus vaccines in national immunization schedules has led to a significant decline in the incidence of these diseases globally. However, challenges remain, including disparities in vaccine coverage and the emergence of non-toxigenic strains causing diphtheria. The review highlights the WHO’s strategies towards achieving higher immunization coverage and the importance of maintaining high vaccination rates to prevent resurgence. Looking forward, the review discusses the ongoing research and development aimed at improving vaccine formulations, reducing adverse reactions, and enhancing the efficacy and durability of protection. Innovations such as nanoparticle vaccines and DNA vaccines are explored as potential avenues for future advancements. Additionally, the review addresses the critical role of global health governance in addressing vaccine hesitancy, improving access in low-resource settings, and coordinating responses to outbreaks. In conclusion, while the battle against diphtheria and tetanus has seen significant victories, continuous efforts in vaccine innovation, policy implementation, and global cooperation are essential to sustain these gains and achieve the ultimate goal of global eradication.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5, From biotechnology to human and planetary health, 4-6 april
T1  - Diphtheria and tetanus vaccines: a historical overview, present achievements, and future directions
EP  - 169
SP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_intor_880
ER  - 

@conference{
author = "Panić, Marko and Prijić, Ivana and Simić, Mihajlo and Ćuruvija, Ivana and Lukić, Ivana and Drgačević, Luka and Kojić, Milan",
year = "2024",
abstract = "Diphtheria and tetanus, once formidable causes of morbidity and mortality worldwide, have seen their threats markedly diminished through the advent and widespread use of vaccines. This review article delves into the historical journey of diphtheria and tetanus vaccines, evaluates their current status in global immunization programs, and explores future perspectives in their evolution and implementation. The inception of diphtheria and tetanus vaccines marked a pivotal shift in infectious disease control. The development of diphtheria toxoid by Emil von Behring in the late 19th century and the subsequent creation of tetanus toxoid in the early 20th century set the stage for large-scale immunization efforts. These efforts were bolstered in the mid-20th century with the integration of these toxoids into combination vaccines, notably the DTP (diphtheria-tetanus-pertussis) vaccine, facilitating broader immunization coverage and enhanced public health outcomes. Currently, the inclusion of diphtheria and tetanus vaccines in national immunization schedules has led to a significant decline in the incidence of these diseases globally. However, challenges remain, including disparities in vaccine coverage and the emergence of non-toxigenic strains causing diphtheria. The review highlights the WHO’s strategies towards achieving higher immunization coverage and the importance of maintaining high vaccination rates to prevent resurgence. Looking forward, the review discusses the ongoing research and development aimed at improving vaccine formulations, reducing adverse reactions, and enhancing the efficacy and durability of protection. Innovations such as nanoparticle vaccines and DNA vaccines are explored as potential avenues for future advancements. Additionally, the review addresses the critical role of global health governance in addressing vaccine hesitancy, improving access in low-resource settings, and coordinating responses to outbreaks. In conclusion, while the battle against diphtheria and tetanus has seen significant victories, continuous efforts in vaccine innovation, policy implementation, and global cooperation are essential to sustain these gains and achieve the ultimate goal of global eradication.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5, From biotechnology to human and planetary health, 4-6 april",
title = "Diphtheria and tetanus vaccines: a historical overview, present achievements, and future directions",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_intor_880"
}

Panić, M., Prijić, I., Simić, M., Ćuruvija, I., Lukić, I., Drgačević, L.,& Kojić, M.. (2024). Diphtheria and tetanus vaccines: a historical overview, present achievements, and future directions. in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5, From biotechnology to human and planetary health, 4-6 april
Serbian Society for Microbiology., 169-169.
https://hdl.handle.net/21.15107/rcub_intor_880

Panić M, Prijić I, Simić M, Ćuruvija I, Lukić I, Drgačević L, Kojić M. Diphtheria and tetanus vaccines: a historical overview, present achievements, and future directions. in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5, From biotechnology to human and planetary health, 4-6 april. 2024;:169-169.
https://hdl.handle.net/21.15107/rcub_intor_880 .

Panić, Marko, Prijić, Ivana, Simić, Mihajlo, Ćuruvija, Ivana, Lukić, Ivana, Drgačević, Luka, Kojić, Milan, "Diphtheria and tetanus vaccines: a historical overview, present achievements, and future directions" in XIII Congress of microbiologists of Serbia with international participation, Mikromed regio 5, From biotechnology to human and planetary health, 4-6 april (2024):169-169,
https://hdl.handle.net/21.15107/rcub_intor_880 .