TY  - CONF
AU  - Pilipović, Ivan
AU  - Vujnović, I.
AU  - Petrović, Raisa
AU  - Kosec, Duško
AU  - Stojić-Vukanić, Z.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/853
AB  - Introduction: It has been suggested that: i) noradrenaline synthesis in ,,adrenergic“ immune cells changes during development of EAE and multiple sclerosis and ii) noradrenaline influences EAE development through a1−adrenoceptor. To elucidate mechanisms standing behind this phenomenon, a1−adrenoceptor−mediated influence of draining lymph node (dLN) cell−derived noradrenaline on CD4+ T−cell response in dLNs from Dark Agouti rats of both sexes immunized for EAE was examined. Methods: Cells recovered from dLNs on 7th day post−immunization were examined for noradrenaline synthesis/content and a1B−adrenoceptor expression using HPLC and/or flow cytometry. Additionally, effects of prazosin (a1- AR blocker) on CD4+ T−cell proliferation, the frequency of IL−17+ CD4+ T−cells and regulatory (Foxp3+CD25+) CD4+ T−cells (Tregs), activational/maturational molecule expression on antigen presenting cells (APCs) and their cytokine profile in dLN cell culture were examined using flow cytometry and/or qRT−PCR/ELISA. Results: Irrespective of sex, conventional CD4+ T−cells and Tregs, and APCs from rat dLNs synthesized noradrenaline, while only Tregs and APCs expressed a1B-adrenoceptor.
In myelin basic protein−stimulated dLN cell cultures from rats of both sexes prazosin increased Treg frequency and Foxp3 expression, but diminished co−stimulatory CD80 and CD86
molecule expression on APCs, thereby reducing CD4+ cell proliferation.
C3  - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands
T1  - Noradrenaline synthesized locally in draining lymph node cells modulates CD4+ T-cell development in rat EAE model: a role for a1-adrenoceptor
SP  - P.A5.06.15
UR  - https://hdl.handle.net/21.15107/rcub_intor_853
ER  - 

@conference{
author = "Pilipović, Ivan and Vujnović, I. and Petrović, Raisa and Kosec, Duško and Stojić-Vukanić, Z. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: It has been suggested that: i) noradrenaline synthesis in ,,adrenergic“ immune cells changes during development of EAE and multiple sclerosis and ii) noradrenaline influences EAE development through a1−adrenoceptor. To elucidate mechanisms standing behind this phenomenon, a1−adrenoceptor−mediated influence of draining lymph node (dLN) cell−derived noradrenaline on CD4+ T−cell response in dLNs from Dark Agouti rats of both sexes immunized for EAE was examined. Methods: Cells recovered from dLNs on 7th day post−immunization were examined for noradrenaline synthesis/content and a1B−adrenoceptor expression using HPLC and/or flow cytometry. Additionally, effects of prazosin (a1- AR blocker) on CD4+ T−cell proliferation, the frequency of IL−17+ CD4+ T−cells and regulatory (Foxp3+CD25+) CD4+ T−cells (Tregs), activational/maturational molecule expression on antigen presenting cells (APCs) and their cytokine profile in dLN cell culture were examined using flow cytometry and/or qRT−PCR/ELISA. Results: Irrespective of sex, conventional CD4+ T−cells and Tregs, and APCs from rat dLNs synthesized noradrenaline, while only Tregs and APCs expressed a1B-adrenoceptor.
In myelin basic protein−stimulated dLN cell cultures from rats of both sexes prazosin increased Treg frequency and Foxp3 expression, but diminished co−stimulatory CD80 and CD86
molecule expression on APCs, thereby reducing CD4+ cell proliferation.",
journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands",
title = "Noradrenaline synthesized locally in draining lymph node cells modulates CD4+ T-cell development in rat EAE model: a role for a1-adrenoceptor",
pages = "P.A5.06.15",
url = "https://hdl.handle.net/21.15107/rcub_intor_853"
}

Pilipović, I., Vujnović, I., Petrović, R., Kosec, D., Stojić-Vukanić, Z.,& Leposavić, G.. (2018). Noradrenaline synthesized locally in draining lymph node cells modulates CD4+ T-cell development in rat EAE model: a role for a1-adrenoceptor. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A5.06.15.
https://hdl.handle.net/21.15107/rcub_intor_853

Pilipović I, Vujnović I, Petrović R, Kosec D, Stojić-Vukanić Z, Leposavić G. Noradrenaline synthesized locally in draining lymph node cells modulates CD4+ T-cell development in rat EAE model: a role for a1-adrenoceptor. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A5.06.15.
https://hdl.handle.net/21.15107/rcub_intor_853 .

Pilipović, Ivan, Vujnović, I., Petrović, Raisa, Kosec, Duško, Stojić-Vukanić, Z., Leposavić, Gordana, "Noradrenaline synthesized locally in draining lymph node cells modulates CD4+ T-cell development in rat EAE model: a role for a1-adrenoceptor" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A5.06.15,
https://hdl.handle.net/21.15107/rcub_intor_853 .

TY  - CONF
AU  - Vujinović, I.
AU  - Pilipović, Ivan
AU  - Petrović, R.
AU  - Stojić−Vukanić, Z.
AU  - Arsenović−Ranin, N.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/852
AB  - Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.
C3  - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands
T1  - Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes
SP  - P.A5.06.07
UR  - https://hdl.handle.net/21.15107/rcub_intor_852
ER  - 

@conference{
author = "Vujinović, I. and Pilipović, Ivan and Petrović, R. and Stojić−Vukanić, Z. and Arsenović−Ranin, N. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.",
journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands",
title = "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes",
pages = "P.A5.06.07",
url = "https://hdl.handle.net/21.15107/rcub_intor_852"
}

Vujinović, I., Pilipović, I., Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N.,& Leposavić, G.. (2018). Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852

Vujinović I, Pilipović I, Petrović R, Stojić−Vukanić Z, Arsenović−Ranin N, Leposavić G. Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852 .

Vujinović, I., Pilipović, Ivan, Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N., Leposavić, Gordana, "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A5.06.07,
https://hdl.handle.net/21.15107/rcub_intor_852 .

TY  - CONF
AU  - Pešić, V.
AU  - Stojić-Vukanić, Z.
AU  - Perišić, M.
AU  - Pilipović, Ivan
AU  - Radojević, K.
AU  - Kosec, Duško
AU  - Arsenović-Ranin, N.
AU  - Leposavić, Gordana
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/847
AB  - We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.
PB  - The Japanese Society for Immunology
PB  - The Japan Society for Clinical Immunology
PB  - Science Council of Japan
PB  - International Union of Immunological Societies
C3  - 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
T1  - Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats
EP  - iv 152
SP  - iv 151
SP  - PP-105-30
UR  - https://hdl.handle.net/21.15107/rcub_intor_847
ER  - 

@conference{
author = "Pešić, V. and Stojić-Vukanić, Z. and Perišić, M. and Pilipović, Ivan and Radojević, K. and Kosec, Duško and Arsenović-Ranin, N. and Leposavić, Gordana",
year = "2010",
abstract = "We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.",
publisher = "The Japanese Society for Immunology, The Japan Society for Clinical Immunology, Science Council of Japan, International Union of Immunological Societies",
journal = "14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan",
title = "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats",
pages = "iv 152-iv 151-PP-105-30",
url = "https://hdl.handle.net/21.15107/rcub_intor_847"
}

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, I., Radojević, K., Kosec, D., Arsenović-Ranin, N.,& Leposavić, G.. (2010). Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
The Japanese Society for Immunology., iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847

Pešić V, Stojić-Vukanić Z, Perišić M, Pilipović I, Radojević K, Kosec D, Arsenović-Ranin N, Leposavić G. Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan. 2010;:iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847 .

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, Ivan, Radojević, K., Kosec, Duško, Arsenović-Ranin, N., Leposavić, Gordana, "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats" in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan (2010):iv 151-iv 152,
https://hdl.handle.net/21.15107/rcub_intor_847 .