Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes
Само за регистроване кориснике
2018
Аутори
Vujinović, I.Pilipović, Ivan
Petrović, R.
Stojić−Vukanić, Z.
Arsenović−Ranin, N.
Leposavić, Gordana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairi...ng migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.
Извор:
Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, 2018, P.A5.06.07-Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Vujinović, I. AU - Pilipović, Ivan AU - Petrović, R. AU - Stojić−Vukanić, Z. AU - Arsenović−Ranin, N. AU - Leposavić, Gordana PY - 2018 UR - http://intor.torlakinstitut.com/handle/123456789/852 AB - Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization. Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development. C3 - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands T1 - Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes SP - P.A5.06.07 UR - https://hdl.handle.net/21.15107/rcub_intor_852 ER -
@conference{ author = "Vujinović, I. and Pilipović, Ivan and Petrović, R. and Stojić−Vukanić, Z. and Arsenović−Ranin, N. and Leposavić, Gordana", year = "2018", abstract = "Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization. Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.", journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands", title = "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes", pages = "P.A5.06.07", url = "https://hdl.handle.net/21.15107/rcub_intor_852" }
Vujinović, I., Pilipović, I., Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N.,& Leposavić, G.. (2018). Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A5.06.07. https://hdl.handle.net/21.15107/rcub_intor_852
Vujinović I, Pilipović I, Petrović R, Stojić−Vukanić Z, Arsenović−Ranin N, Leposavić G. Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A5.06.07. https://hdl.handle.net/21.15107/rcub_intor_852 .
Vujinović, I., Pilipović, Ivan, Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N., Leposavić, Gordana, "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A5.06.07, https://hdl.handle.net/21.15107/rcub_intor_852 .