TY  - JOUR
AU  - Begović, Jelena
AU  - Jovčić, Branko
AU  - Papić-Obradović, Milena
AU  - Veljović, Katarina
AU  - Lukić, Jovanka
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/750
AB  - Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential.
PB  - Elsevier Gmbh, Munich
T2  - Microbiological Research
T1  - Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk
EP  - 83
IS  - 2
SP  - 77
VL  - 168
DO  - 10.1016/j.micres.2012.09.004
ER  - 

@article{
author = "Begović, Jelena and Jovčić, Branko and Papić-Obradović, Milena and Veljović, Katarina and Lukić, Jovanka and Kojić, Milan and Topisirović, Ljubiša",
year = "2013",
abstract = "Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential.",
publisher = "Elsevier Gmbh, Munich",
journal = "Microbiological Research",
title = "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk",
pages = "83-77",
number = "2",
volume = "168",
doi = "10.1016/j.micres.2012.09.004"
}

Begović, J., Jovčić, B., Papić-Obradović, M., Veljović, K., Lukić, J., Kojić, M.,& Topisirović, L.. (2013). Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research
Elsevier Gmbh, Munich., 168(2), 77-83.
https://doi.org/10.1016/j.micres.2012.09.004

Begović J, Jovčić B, Papić-Obradović M, Veljović K, Lukić J, Kojić M, Topisirović L. Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research. 2013;168(2):77-83.
doi:10.1016/j.micres.2012.09.004 .

Begović, Jelena, Jovčić, Branko, Papić-Obradović, Milena, Veljović, Katarina, Lukić, Jovanka, Kojić, Milan, Topisirović, Ljubiša, "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk" in Microbiological Research, 168, no. 2 (2013):77-83,
https://doi.org/10.1016/j.micres.2012.09.004 . .