Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk
Аутори
Begović, JelenaJovčić, Branko
Papić-Obradović, Milena
Veljović, Katarina
Lukić, Jovanka
Kojić, Milan
Topisirović, Ljubiša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/ad...hesin AtLE, point to their pathogenic potential.
Кључне речи:
Virulence / S. epidermidis / Human breast milk / Antibiotic susceptibilityИзвор:
Microbiological Research, 2013, 168, 2, 77-83Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1016/j.micres.2012.09.004
ISSN: 0944-5013
PubMed: 23098640
WoS: 000315179400003
Scopus: 2-s2.0-84872598109
Институција/група
TorlakTY - JOUR AU - Begović, Jelena AU - Jovčić, Branko AU - Papić-Obradović, Milena AU - Veljović, Katarina AU - Lukić, Jovanka AU - Kojić, Milan AU - Topisirović, Ljubiša PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/750 AB - Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential. PB - Elsevier Gmbh, Munich T2 - Microbiological Research T1 - Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk EP - 83 IS - 2 SP - 77 VL - 168 DO - 10.1016/j.micres.2012.09.004 ER -
@article{ author = "Begović, Jelena and Jovčić, Branko and Papić-Obradović, Milena and Veljović, Katarina and Lukić, Jovanka and Kojić, Milan and Topisirović, Ljubiša", year = "2013", abstract = "Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential.", publisher = "Elsevier Gmbh, Munich", journal = "Microbiological Research", title = "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk", pages = "83-77", number = "2", volume = "168", doi = "10.1016/j.micres.2012.09.004" }
Begović, J., Jovčić, B., Papić-Obradović, M., Veljović, K., Lukić, J., Kojić, M.,& Topisirović, L.. (2013). Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research Elsevier Gmbh, Munich., 168(2), 77-83. https://doi.org/10.1016/j.micres.2012.09.004
Begović J, Jovčić B, Papić-Obradović M, Veljović K, Lukić J, Kojić M, Topisirović L. Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research. 2013;168(2):77-83. doi:10.1016/j.micres.2012.09.004 .
Begović, Jelena, Jovčić, Branko, Papić-Obradović, Milena, Veljović, Katarina, Lukić, Jovanka, Kojić, Milan, Topisirović, Ljubiša, "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk" in Microbiological Research, 168, no. 2 (2013):77-83, https://doi.org/10.1016/j.micres.2012.09.004 . .