TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić-Redžić, V.
AU  - Radulović, Jelena
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/124
AB  - We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Immunopharmacology
T1  - Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors
EP  - 262
IS  - 3
SP  - 255
VL  - 49
DO  - 10.1016/S0162-3109(00)00213-7
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić-Redžić, V. and Radulović, Jelena",
year = "2000",
abstract = "We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Immunopharmacology",
title = "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors",
pages = "262-255",
number = "3",
volume = "49",
doi = "10.1016/S0162-3109(00)00213-7"
}

Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T., Vujić-Redžić, V.,& Radulović, J.. (2000). Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology
Elsevier, Amsterdam., 49(3), 255-262.
https://doi.org/10.1016/S0162-3109(00)00213-7

Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Vujić-Redžić V, Radulović J. Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology. 2000;49(3):255-262.
doi:10.1016/S0162-3109(00)00213-7 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić-Redžić, V., Radulović, Jelena, "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors" in Immunopharmacology, 49, no. 3 (2000):255-262,
https://doi.org/10.1016/S0162-3109(00)00213-7 . .

TY  - JOUR
AU  - Vujić-Redžić, V.
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Radulović, Jelena
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/115
AB  - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat
EP  - 77
IS  - 2
SP  - 70
VL  - 8
DO  - 10.1159/000026455
ER  - 

@article{
author = "Vujić-Redžić, V. and Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Radulović, Jelena",
year = "2000",
abstract = "Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat",
pages = "77-70",
number = "2",
volume = "8",
doi = "10.1159/000026455"
}

Vujić-Redžić, V., Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T.,& Radulović, J.. (2000). Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation
Karger, Basel., 8(2), 70-77.
https://doi.org/10.1159/000026455

Vujić-Redžić V, Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Radulović J. Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation. 2000;8(2):70-77.
doi:10.1159/000026455 .

Vujić-Redžić, V., Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Radulović, Jelena, "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat" in Neuroimmunomodulation, 8, no. 2 (2000):70-77,
https://doi.org/10.1159/000026455 . .