InTOR - Repository of the Institute “Torlak”
Institute of Virology, Vaccines and Sera “Torlak”
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors

Authorized Users Only
2000
Authors
Dimitrijević, Mirjana
Stanojević, Stanislava
Kovačević-Jovanović, Vesna
Miletić, Tatjana
Vujić-Redžić, V.
Radulović, Jelena
Article (Published version)
Metadata
Show full item record
Abstract
We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenou...s opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.

Keywords:
methionine-enkephalin / leucine-enkephalin / opioid receptors, OF1, OP2, OF3 / plaque-forming cell response / central nervous system
Source:
Immunopharmacology, 2000, 49, 3, 255-262
Publisher:
  • Elsevier, Amsterdam

DOI: 10.1016/S0162-3109(00)00213-7

ISSN: 0162-3109

PubMed: 10996023

WoS: 000089568600004

Scopus: 2-s2.0-0033829010
[ Google Scholar ]
13
10
URI
http://intor.torlakinstitut.com/handle/123456789/124
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić-Redžić, V.
AU  - Radulović, Jelena
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/124
AB  - We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Immunopharmacology
T1  - Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors
EP  - 262
IS  - 3
SP  - 255
VL  - 49
DO  - 10.1016/S0162-3109(00)00213-7
UR  - conv_119
ER  - 
@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić-Redžić, V. and Radulović, Jelena",
year = "2000",
abstract = "We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Immunopharmacology",
title = "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors",
pages = "262-255",
number = "3",
volume = "49",
doi = "10.1016/S0162-3109(00)00213-7",
url = "conv_119"
}
Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T., Vujić-Redžić, V.,& Radulović, J.. (2000). Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology
Elsevier, Amsterdam., 49(3), 255-262.
https://doi.org/10.1016/S0162-3109(00)00213-7
conv_119
Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Vujić-Redžić V, Radulović J. Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology. 2000;49(3):255-262.
doi:10.1016/S0162-3109(00)00213-7
conv_119 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić-Redžić, V., Radulović, Jelena, "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors" in Immunopharmacology, 49, no. 3 (2000):255-262,
https://doi.org/10.1016/S0162-3109(00)00213-7 .,
conv_119 .

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB