TY  - CONF
AU  - Bufan, B.
AU  - Arsenović-Ranin, N.
AU  - Dimitrijević, M.
AU  - Nacka-Aleksić, M.
AU  - Kosec, Duško
AU  - Pilipović, Ivan
AU  - Stojanović, M.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/850
AB  - Introduction: Considering sex bias in rheumatoid arthritis prevalence, influence of biological sex on the disease development in Dark Agouti rat collagen II (CII)−induced arthritis (CIA) model of the human disease was examined. Methods: Sex bias in CD4+ T cell responses in inguinal (draining the site of immunization in preclinical CIA) and popliteal (draining inflamed joints at the peak of CIA) lymph nodes (LNs) and mechanisms controlling their development were examined using flow cytometry and/or ELISA/qRT−PCR. Results: In both inguinal and popliteal LNs greater number of CD4+CD25+Foxp3− cells, presumably activated effector T cells, was found in females compared with males, and they exhibited greater CII−specific proliferation. Consistently, more IL−17+, IFN−γ+ and IL−17+IFN−γ+ T cells were retrieved from both inguinal and popliteal female rat LNs. Moreover, more GM−CSF+ and IL−17+IFN−γ+GM−CSF+ T cells were retrieved from female compared with male rat popliteal LNs. On the other hand, lower frequency of PD−1+ cells among CD4+CD25+Foxp3+ regulatory T cells (Tregs) from female popliteal and inguinal LNs suggested lower suppressive capacity of their Tregs. Additionally, from female rat popliteal LNs fewer Tregs were recovered. Furthermore, the number of regulatory LN B10 cells was lower in females. Moreover, compared with males, in females was shifted LN INF−γ+/IL−4+ T−cell ratio towards the former, and accordingly serum CII−specific IgG2a/IgG1 antibody ratio was shifted towards pathogenic IgG2a antibodies. Conclusion: The study suggests that a less efficient control of (auto)immune Th1/Th17 cell responses during CIA development contributes to sex bias in the susceptibility to CIA.
C3  - 5th European Congress of Immunology, Septembar 2-5, Abstract Book
T1  - Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis
SP  - P.C1.01.02
UR  - https://hdl.handle.net/21.15107/rcub_intor_850
ER  - 

@conference{
author = "Bufan, B. and Arsenović-Ranin, N. and Dimitrijević, M. and Nacka-Aleksić, M. and Kosec, Duško and Pilipović, Ivan and Stojanović, M. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: Considering sex bias in rheumatoid arthritis prevalence, influence of biological sex on the disease development in Dark Agouti rat collagen II (CII)−induced arthritis (CIA) model of the human disease was examined. Methods: Sex bias in CD4+ T cell responses in inguinal (draining the site of immunization in preclinical CIA) and popliteal (draining inflamed joints at the peak of CIA) lymph nodes (LNs) and mechanisms controlling their development were examined using flow cytometry and/or ELISA/qRT−PCR. Results: In both inguinal and popliteal LNs greater number of CD4+CD25+Foxp3− cells, presumably activated effector T cells, was found in females compared with males, and they exhibited greater CII−specific proliferation. Consistently, more IL−17+, IFN−γ+ and IL−17+IFN−γ+ T cells were retrieved from both inguinal and popliteal female rat LNs. Moreover, more GM−CSF+ and IL−17+IFN−γ+GM−CSF+ T cells were retrieved from female compared with male rat popliteal LNs. On the other hand, lower frequency of PD−1+ cells among CD4+CD25+Foxp3+ regulatory T cells (Tregs) from female popliteal and inguinal LNs suggested lower suppressive capacity of their Tregs. Additionally, from female rat popliteal LNs fewer Tregs were recovered. Furthermore, the number of regulatory LN B10 cells was lower in females. Moreover, compared with males, in females was shifted LN INF−γ+/IL−4+ T−cell ratio towards the former, and accordingly serum CII−specific IgG2a/IgG1 antibody ratio was shifted towards pathogenic IgG2a antibodies. Conclusion: The study suggests that a less efficient control of (auto)immune Th1/Th17 cell responses during CIA development contributes to sex bias in the susceptibility to CIA.",
journal = "5th European Congress of Immunology, Septembar 2-5, Abstract Book",
title = "Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis",
pages = "P.C1.01.02",
url = "https://hdl.handle.net/21.15107/rcub_intor_850"
}

Bufan, B., Arsenović-Ranin, N., Dimitrijević, M., Nacka-Aleksić, M., Kosec, D., Pilipović, I., Stojanović, M.,& Leposavić, G.. (2018). Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis. in 5th European Congress of Immunology, Septembar 2-5, Abstract Book, P.C1.01.02.
https://hdl.handle.net/21.15107/rcub_intor_850

Bufan B, Arsenović-Ranin N, Dimitrijević M, Nacka-Aleksić M, Kosec D, Pilipović I, Stojanović M, Leposavić G. Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis. in 5th European Congress of Immunology, Septembar 2-5, Abstract Book. 2018;:P.C1.01.02.
https://hdl.handle.net/21.15107/rcub_intor_850 .

Bufan, B., Arsenović-Ranin, N., Dimitrijević, M., Nacka-Aleksić, M., Kosec, Duško, Pilipović, Ivan, Stojanović, M., Leposavić, Gordana, "Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis" in 5th European Congress of Immunology, Septembar 2-5, Abstract Book (2018):P.C1.01.02,
https://hdl.handle.net/21.15107/rcub_intor_850 .

TY  - CONF
AU  - Vujinović, I.
AU  - Pilipović, Ivan
AU  - Petrović, R.
AU  - Stojić−Vukanić, Z.
AU  - Arsenović−Ranin, N.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/852
AB  - Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.
C3  - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands
T1  - Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes
SP  - P.A5.06.07
UR  - https://hdl.handle.net/21.15107/rcub_intor_852
ER  - 

@conference{
author = "Vujinović, I. and Pilipović, Ivan and Petrović, R. and Stojić−Vukanić, Z. and Arsenović−Ranin, N. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.",
journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands",
title = "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes",
pages = "P.A5.06.07",
url = "https://hdl.handle.net/21.15107/rcub_intor_852"
}

Vujinović, I., Pilipović, I., Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N.,& Leposavić, G.. (2018). Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852

Vujinović I, Pilipović I, Petrović R, Stojić−Vukanić Z, Arsenović−Ranin N, Leposavić G. Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852 .

Vujinović, I., Pilipović, Ivan, Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N., Leposavić, Gordana, "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A5.06.07,
https://hdl.handle.net/21.15107/rcub_intor_852 .

TY  - CONF
AU  - Pešić, V.
AU  - Stojić-Vukanić, Z.
AU  - Perišić, M.
AU  - Pilipović, Ivan
AU  - Radojević, K.
AU  - Kosec, Duško
AU  - Arsenović-Ranin, N.
AU  - Leposavić, Gordana
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/847
AB  - We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.
PB  - The Japanese Society for Immunology
PB  - The Japan Society for Clinical Immunology
PB  - Science Council of Japan
PB  - International Union of Immunological Societies
C3  - 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
T1  - Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats
EP  - iv 152
SP  - iv 151
SP  - PP-105-30
UR  - https://hdl.handle.net/21.15107/rcub_intor_847
ER  - 

@conference{
author = "Pešić, V. and Stojić-Vukanić, Z. and Perišić, M. and Pilipović, Ivan and Radojević, K. and Kosec, Duško and Arsenović-Ranin, N. and Leposavić, Gordana",
year = "2010",
abstract = "We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.",
publisher = "The Japanese Society for Immunology, The Japan Society for Clinical Immunology, Science Council of Japan, International Union of Immunological Societies",
journal = "14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan",
title = "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats",
pages = "iv 152-iv 151-PP-105-30",
url = "https://hdl.handle.net/21.15107/rcub_intor_847"
}

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, I., Radojević, K., Kosec, D., Arsenović-Ranin, N.,& Leposavić, G.. (2010). Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
The Japanese Society for Immunology., iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847

Pešić V, Stojić-Vukanić Z, Perišić M, Pilipović I, Radojević K, Kosec D, Arsenović-Ranin N, Leposavić G. Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan. 2010;:iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847 .

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, Ivan, Radojević, K., Kosec, Duško, Arsenović-Ranin, N., Leposavić, Gordana, "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats" in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan (2010):iv 151-iv 152,
https://hdl.handle.net/21.15107/rcub_intor_847 .