TY  - JOUR
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Topisirović, Ljubiša
AU  - Degrassi, Giuliano
AU  - Myers, Michael
AU  - Venturi, Vittorio
AU  - Fira, Đorđe
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/574
UR  - http://intor.torlakinstitut.com/handle/123456789/691
AB  - Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria
EP  - 31
IS  - 1
SP  - 25
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_imagine_574
ER  - 

@article{
author = "Berić, Tanja and Kojić, Milan and Stanković, Slaviša and Topisirović, Ljubiša and Degrassi, Giuliano and Myers, Michael and Venturi, Vittorio and Fira, Đorđe",
year = "2012",
abstract = "Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria",
pages = "31-25",
number = "1",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_imagine_574"
}

Berić, T., Kojić, M., Stanković, S., Topisirović, L., Degrassi, G., Myers, M., Venturi, V.,& Fira, Đ.. (2012). Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology
University of Zagreb., 50(1), 25-31.
https://hdl.handle.net/21.15107/rcub_imagine_574

Berić T, Kojić M, Stanković S, Topisirović L, Degrassi G, Myers M, Venturi V, Fira Đ. Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria. in Food Technology and Biotechnology. 2012;50(1):25-31.
https://hdl.handle.net/21.15107/rcub_imagine_574 .

Berić, Tanja, Kojić, Milan, Stanković, Slaviša, Topisirović, Ljubiša, Degrassi, Giuliano, Myers, Michael, Venturi, Vittorio, Fira, Đorđe, "Antimicrobial Activity of Bacillus sp Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria" in Food Technology and Biotechnology, 50, no. 1 (2012):25-31,
https://hdl.handle.net/21.15107/rcub_imagine_574 .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Venturi, Vittorio
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/534
UR  - http://intor.torlakinstitut.com/handle/123456789/827
AB  - Pseudomonas sp. strain ATCC19151 is a natural isolate from sewage with the ability to degrade detergents. Genes encoding potential choline sulfatase (betC), substrate-binding ABC transporter protein (betD), sulfate transporter (betE), and divergent putative transcriptional regulator (betR) were cloned and characterized from strain ATCC19151. In silico analysis revealed that (1) the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I, (2) BetR belongs to the LysR family of transcriptional regulators, (3) BetD is part of the PBPb superfamily of periplasmic and membrane-associated proteins, and (4) BetE is a permease and contains STAS domain. Insertional mutagenesis and genetic complementation show that betC gene encodes a functional choline sulfatase. Analysis of the betC (P (betC) ) and betR (P (betR) ) promoters revealed that they are inducible. BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate, BetR represses its own transcription. It was further established that BetR directly binds to betC-betR intergenic region in vitro, with higher affinity in the presence of choline sulfate as cofactor. Transcription of betC and betR was not induced in the presence of high concentration of NaCl.
PB  - Springer, New York
T2  - Archives of Microbiology
T1  - Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151
EP  - 405
IS  - 6
SP  - 399
VL  - 193
DO  - 10.1007/s00203-011-0685-x
ER  - 

@article{
author = "Jovčić, Branko and Venturi, Vittorio and Topisirović, Ljubiša and Kojić, Milan",
year = "2011",
abstract = "Pseudomonas sp. strain ATCC19151 is a natural isolate from sewage with the ability to degrade detergents. Genes encoding potential choline sulfatase (betC), substrate-binding ABC transporter protein (betD), sulfate transporter (betE), and divergent putative transcriptional regulator (betR) were cloned and characterized from strain ATCC19151. In silico analysis revealed that (1) the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I, (2) BetR belongs to the LysR family of transcriptional regulators, (3) BetD is part of the PBPb superfamily of periplasmic and membrane-associated proteins, and (4) BetE is a permease and contains STAS domain. Insertional mutagenesis and genetic complementation show that betC gene encodes a functional choline sulfatase. Analysis of the betC (P (betC) ) and betR (P (betR) ) promoters revealed that they are inducible. BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate, BetR represses its own transcription. It was further established that BetR directly binds to betC-betR intergenic region in vitro, with higher affinity in the presence of choline sulfate as cofactor. Transcription of betC and betR was not induced in the presence of high concentration of NaCl.",
publisher = "Springer, New York",
journal = "Archives of Microbiology",
title = "Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151",
pages = "405-399",
number = "6",
volume = "193",
doi = "10.1007/s00203-011-0685-x"
}

Jovčić, B., Venturi, V., Topisirović, L.,& Kojić, M.. (2011). Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151. in Archives of Microbiology
Springer, New York., 193(6), 399-405.
https://doi.org/10.1007/s00203-011-0685-x

Jovčić B, Venturi V, Topisirović L, Kojić M. Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151. in Archives of Microbiology. 2011;193(6):399-405.
doi:10.1007/s00203-011-0685-x .

Jovčić, Branko, Venturi, Vittorio, Topisirović, Ljubiša, Kojić, Milan, "Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151" in Archives of Microbiology, 193, no. 6 (2011):399-405,
https://doi.org/10.1007/s00203-011-0685-x . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Bertani, Iris
AU  - Venturi, Vittorio
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/333
UR  - http://intor.torlakinstitut.com/handle/123456789/679
AB  - The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.
PB  - Microbiology Soc Korea, Seoul
T2  - Journal of Microbiology
T1  - 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation
EP  - 61
IS  - 1
SP  - 56
VL  - 46
DO  - 10.1007/s12275-007-0127-2
ER  - 

@article{
author = "Jovčić, Branko and Bertani, Iris and Venturi, Vittorio and Topisirović, Ljubiša and Kojić, Milan",
year = "2008",
abstract = "The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.",
publisher = "Microbiology Soc Korea, Seoul",
journal = "Journal of Microbiology",
title = "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation",
pages = "61-56",
number = "1",
volume = "46",
doi = "10.1007/s12275-007-0127-2"
}

Jovčić, B., Bertani, I., Venturi, V., Topisirović, L.,& Kojić, M.. (2008). 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology
Microbiology Soc Korea, Seoul., 46(1), 56-61.
https://doi.org/10.1007/s12275-007-0127-2

Jovčić B, Bertani I, Venturi V, Topisirović L, Kojić M. 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology. 2008;46(1):56-61.
doi:10.1007/s12275-007-0127-2 .

Jovčić, Branko, Bertani, Iris, Venturi, Vittorio, Topisirović, Ljubiša, Kojić, Milan, "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation" in Journal of Microbiology, 46, no. 1 (2008):56-61,
https://doi.org/10.1007/s12275-007-0127-2 . .