TY  - JOUR
AU  - Begović, Jelena
AU  - Huys, Geert
AU  - Mayo, Baltasar
AU  - D'Haene, Klaas
AU  - Belen Florez, Ana
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/748
AB  - Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
EP  - 426
IS  - 6
SP  - 421
VL  - 160
DO  - 10.1016/j.resmic.2009.07.005
ER  - 

@article{
author = "Begović, Jelena and Huys, Geert and Mayo, Baltasar and D'Haene, Klaas and Belen Florez, Ana and Lozo, Jelena and Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša",
year = "2009",
abstract = "Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance",
pages = "426-421",
number = "6",
volume = "160",
doi = "10.1016/j.resmic.2009.07.005"
}

Begović, J., Huys, G., Mayo, B., D'Haene, K., Belen Florez, A., Lozo, J., Kojić, M., Strahinić, I.,& Topisirović, L.. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology
Elsevier, Amsterdam., 160(6), 421-426.
https://doi.org/10.1016/j.resmic.2009.07.005

Begović J, Huys G, Mayo B, D'Haene K, Belen Florez A, Lozo J, Kojić M, Strahinić I, Topisirović L. Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology. 2009;160(6):421-426.
doi:10.1016/j.resmic.2009.07.005 .

Begović, Jelena, Huys, Geert, Mayo, Baltasar, D'Haene, Klaas, Belen Florez, Ana, Lozo, Jelena, Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance" in Research in Microbiology, 160, no. 6 (2009):421-426,
https://doi.org/10.1016/j.resmic.2009.07.005 . .

TY  - JOUR
AU  - Begović, Jelena
AU  - Huys, Geert
AU  - Mayo, Baltasar
AU  - D'Haene, Klaas
AU  - Belen Florez, Ana
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/749
AB  - Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.
PB  - Elsevier
T2  - Research in Microbiology
T1  - Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
EP  - 426
IS  - 6
SP  - 421
VL  - 160
DO  - 10.1016/j.resmic.2009.07.005
ER  - 

@article{
author = "Begović, Jelena and Huys, Geert and Mayo, Baltasar and D'Haene, Klaas and Belen Florez, Ana and Lozo, Jelena and Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša",
year = "2009",
abstract = "Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.",
publisher = "Elsevier",
journal = "Research in Microbiology",
title = "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance",
pages = "426-421",
number = "6",
volume = "160",
doi = "10.1016/j.resmic.2009.07.005"
}

Begović, J., Huys, G., Mayo, B., D'Haene, K., Belen Florez, A., Lozo, J., Kojić, M., Strahinić, I.,& Topisirović, L.. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology
Elsevier., 160(6), 421-426.
https://doi.org/10.1016/j.resmic.2009.07.005

Begović J, Huys G, Mayo B, D'Haene K, Belen Florez A, Lozo J, Kojić M, Strahinić I, Topisirović L. Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology. 2009;160(6):421-426.
doi:10.1016/j.resmic.2009.07.005 .

Begović, Jelena, Huys, Geert, Mayo, Baltasar, D'Haene, Klaas, Belen Florez, Ana, Lozo, Jelena, Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance" in Research in Microbiology, 160, no. 6 (2009):421-426,
https://doi.org/10.1016/j.resmic.2009.07.005 . .