Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
Аутори
Begović, JelenaHuys, Geert
Mayo, Baltasar
D'Haene, Klaas
Belen Florez, Ana
Lozo, Jelena
Kojić, Milan
Strahinić, Ivana
Topisirović, Ljubiša
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g ...mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.
Кључне речи:
Vaginal lactobacilli / Lactobacillus rhamnosus / Erythromycin resistance / 23S rRNA mutationИзвор:
Research in Microbiology, 2009, 160, 6, 421-426Издавач:
- Elsevier
Финансирање / пројекти:
- Изучавање регулације експресије гена одабраних индустријских микроорганизама (RS-MESTD-MPN2006-2010-143036)
- EU project within the VI Frame Program (ACE-ART, ref. CT-2003-506214)
Напомена:
- This is the peer reviewed version of the paper: Begovic, J., Huys, G., Mayo, B., D’Haene, K., Florez, A. B., Lozo, J., Kojic, M., Strahinic, I., & Topisirovic, L. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. Research in Microbiology, 160(6), 421–426. https://doi.org/10.1016/j.resmic.2009.07.005
- Published version: http://intor.torlakinstitut.com/handle/123456789/748
Повезане информације:
- Друга верзија
https://doi.org/10.1016/j.resmic.2009.07.005 - Друга верзија
http://intor.torlakinstitut.com/handle/123456789/748
DOI: 10.1016/j.resmic.2009.07.005
ISSN: 0923-2508
PubMed: 19619642
WoS: 000270162300008
Scopus: 2-s2.0-69249213404
Институција/група
TorlakTY - JOUR AU - Begović, Jelena AU - Huys, Geert AU - Mayo, Baltasar AU - D'Haene, Klaas AU - Belen Florez, Ana AU - Lozo, Jelena AU - Kojić, Milan AU - Strahinić, Ivana AU - Topisirović, Ljubiša PY - 2009 UR - http://intor.torlakinstitut.com/handle/123456789/749 AB - Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli. PB - Elsevier T2 - Research in Microbiology T1 - Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance EP - 426 IS - 6 SP - 421 VL - 160 DO - 10.1016/j.resmic.2009.07.005 ER -
@article{ author = "Begović, Jelena and Huys, Geert and Mayo, Baltasar and D'Haene, Klaas and Belen Florez, Ana and Lozo, Jelena and Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša", year = "2009", abstract = "Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.", publisher = "Elsevier", journal = "Research in Microbiology", title = "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance", pages = "426-421", number = "6", volume = "160", doi = "10.1016/j.resmic.2009.07.005" }
Begović, J., Huys, G., Mayo, B., D'Haene, K., Belen Florez, A., Lozo, J., Kojić, M., Strahinić, I.,& Topisirović, L.. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology Elsevier., 160(6), 421-426. https://doi.org/10.1016/j.resmic.2009.07.005
Begović J, Huys G, Mayo B, D'Haene K, Belen Florez A, Lozo J, Kojić M, Strahinić I, Topisirović L. Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology. 2009;160(6):421-426. doi:10.1016/j.resmic.2009.07.005 .
Begović, Jelena, Huys, Geert, Mayo, Baltasar, D'Haene, Klaas, Belen Florez, Ana, Lozo, Jelena, Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance" in Research in Microbiology, 160, no. 6 (2009):421-426, https://doi.org/10.1016/j.resmic.2009.07.005 . .