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Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes
dc.creator | Vujinović, I. | |
dc.creator | Pilipović, Ivan | |
dc.creator | Petrović, R. | |
dc.creator | Stojić−Vukanić, Z. | |
dc.creator | Arsenović−Ranin, N. | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2023-11-29T14:07:54Z | |
dc.date.available | 2023-11-29T14:07:54Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/852 | |
dc.description.abstract | Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization. Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development. | sr |
dc.language.iso | en | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands | sr |
dc.title | Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.spage | P.A5.06.07 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_intor_852 | |
dc.type.version | publishedVersion | sr |