Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50
Abstract
A large chromosomal inversion that confers resistance to high concentrations of the antibiotic spectinomycin in Lactococcus lactis subsp. lactis bv. diacetylactis S50 was identified by pulsed field gel electrophoresis. The same type of inversion was identified in 4 independent experiments and in 4 different derivatives of strain S50, indicating the same position and the same mechanism of recombination as a response to antibiotic selective pressure in all derivatives. An analysis of ribosomal operons in strain S50 and mutants revealed that ribosomal operons are not endpoints of the recombination. Spectinomycin-resistant mutants appeared in a population of S50 derivatives at a high frequency of 2 x 10(-7). These spectinomycin-resistant mutants were not able to compete successfully with the wild-type strain during 25 generations (48 h) of co-culture in vitro, indicating that inversion had a significant fitness cost. Results demonstrate that as a mechanism of genome plasticity, inversion c...an be directly involved in one-step development of the adaptation to a high concentration of spectinomycin.
Keywords:
spectinomycin resistance / PFGE / lactococcus / chromosomal inversionSource:
Canadian Journal of Microbiology, 2008, 54, 2, 143-149Publisher:
- Natl Research Council Canada-N R C Research Press, Ottawa
DOI: 10.1139/W07-121
ISSN: 0008-4166
PubMed: 18388984
WoS: 000254702200009
Scopus: 2-s2.0-39749114910
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TorlakTY - JOUR AU - Kojić, Milan AU - Jovčić, Branko AU - Begović, Jelena AU - Fira, Đorđe AU - Topisirović, Ljubiša PY - 2008 UR - http://intor.torlakinstitut.com/handle/123456789/819 AB - A large chromosomal inversion that confers resistance to high concentrations of the antibiotic spectinomycin in Lactococcus lactis subsp. lactis bv. diacetylactis S50 was identified by pulsed field gel electrophoresis. The same type of inversion was identified in 4 independent experiments and in 4 different derivatives of strain S50, indicating the same position and the same mechanism of recombination as a response to antibiotic selective pressure in all derivatives. An analysis of ribosomal operons in strain S50 and mutants revealed that ribosomal operons are not endpoints of the recombination. Spectinomycin-resistant mutants appeared in a population of S50 derivatives at a high frequency of 2 x 10(-7). These spectinomycin-resistant mutants were not able to compete successfully with the wild-type strain during 25 generations (48 h) of co-culture in vitro, indicating that inversion had a significant fitness cost. Results demonstrate that as a mechanism of genome plasticity, inversion can be directly involved in one-step development of the adaptation to a high concentration of spectinomycin. PB - Natl Research Council Canada-N R C Research Press, Ottawa T2 - Canadian Journal of Microbiology T1 - Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50 EP - 149 IS - 2 SP - 143 VL - 54 DO - 10.1139/W07-121 ER -
@article{ author = "Kojić, Milan and Jovčić, Branko and Begović, Jelena and Fira, Đorđe and Topisirović, Ljubiša", year = "2008", abstract = "A large chromosomal inversion that confers resistance to high concentrations of the antibiotic spectinomycin in Lactococcus lactis subsp. lactis bv. diacetylactis S50 was identified by pulsed field gel electrophoresis. The same type of inversion was identified in 4 independent experiments and in 4 different derivatives of strain S50, indicating the same position and the same mechanism of recombination as a response to antibiotic selective pressure in all derivatives. An analysis of ribosomal operons in strain S50 and mutants revealed that ribosomal operons are not endpoints of the recombination. Spectinomycin-resistant mutants appeared in a population of S50 derivatives at a high frequency of 2 x 10(-7). These spectinomycin-resistant mutants were not able to compete successfully with the wild-type strain during 25 generations (48 h) of co-culture in vitro, indicating that inversion had a significant fitness cost. Results demonstrate that as a mechanism of genome plasticity, inversion can be directly involved in one-step development of the adaptation to a high concentration of spectinomycin.", publisher = "Natl Research Council Canada-N R C Research Press, Ottawa", journal = "Canadian Journal of Microbiology", title = "Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50", pages = "149-143", number = "2", volume = "54", doi = "10.1139/W07-121" }
Kojić, M., Jovčić, B., Begović, J., Fira, Đ.,& Topisirović, L.. (2008). Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology Natl Research Council Canada-N R C Research Press, Ottawa., 54(2), 143-149. https://doi.org/10.1139/W07-121
Kojić M, Jovčić B, Begović J, Fira Đ, Topisirović L. Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology. 2008;54(2):143-149. doi:10.1139/W07-121 .
Kojić, Milan, Jovčić, Branko, Begović, Jelena, Fira, Đorđe, Topisirović, Ljubiša, "Large chromosomal inversion correlated with spectinomycin resistance in Lactococcus lactis subsp lactis bv. diacetylactis S50" in Canadian Journal of Microbiology, 54, no. 2 (2008):143-149, https://doi.org/10.1139/W07-121 . .