Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue
Samo za registrovane korisnike
2014
Autori
Hidalgo-Cantabrana, ClaudioŽivković, Milica
Lopez, Patricia
Suarez, Ana
Miljković, Marija
Kojić, Milan
Margolles, Abelardo
Golić, Nataša
Ruas-Madiedo, Patricia
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The effect of exopolysaccharide (EPS) producing bifidobacteria, and the EPS derived thereof, on the modulation of immune response was evaluated. Cells isolated from gut associated lymphoid tissue (GALT) and from peripheral blood mononuclear cells (PBMC) of naive rats were used. The proliferation and cytokine production of these immune cells in the presence of the three isogenic Bifidobacterium animalis subsp. lactis strains (A1, A1dOx and A1dOxR), as well as their purified polymers, were in vitro analysed. The cytokine pattern produced by immune cells isolated from GALT showed that most levels remained stable in the presence of the three strains or their corresponding polymers. However, in PBMC the UV-inactivated bacteria induced higher levels of the ratios IFN gamma/IL-17, TNF alpha/IL-10 and TNF alpha/TGF beta, and no variation in the ratio IFN gamma/IL-4. Thus, B. animalis subsp. lactis strains were able to activate blood monocytes as well as T lymphocytes towards a mild inflammator...y Th1 response. Furthermore, only the EPS-A1dOxR was able to stimulate a response in a similar way than its EPS-producing bacterium. Our work supports the notion that some bifidobacterial EPS could play a role in mediating the dialog of these microorganisms with the immune system. In addition, this study emphasizes the effect that the origin of the immune cells has in results obtained; this could explain the great amount of contradiction found in literature about the immunomodulation capability of EPS from probiotic bacteria.
Ključne reči:
PBMC / Immune response / GALT / Exopolysaccharide / Cytokine / BifidobacteriaIzvor:
Anaerobe, 2014, 26, 24-30Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Spanish Ministry of Science and Innovation (MICINN)
- FEDER European Union [AGL2009-09445]
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
- bilateral collaboration project [AIB2010SE-00386]
- FPI fellowship
DOI: 10.1016/j.anaerobe.2014.01.003
ISSN: 1075-9964
PubMed: 24445155
WoS: 000333510700005
Scopus: 2-s2.0-84893039270
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/778http://intor.torlakinstitut.com/handle/123456789/739
Institucija/grupa
TorlakTY - JOUR AU - Hidalgo-Cantabrana, Claudio AU - Živković, Milica AU - Lopez, Patricia AU - Suarez, Ana AU - Miljković, Marija AU - Kojić, Milan AU - Margolles, Abelardo AU - Golić, Nataša AU - Ruas-Madiedo, Patricia PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/778 UR - http://intor.torlakinstitut.com/handle/123456789/739 AB - The effect of exopolysaccharide (EPS) producing bifidobacteria, and the EPS derived thereof, on the modulation of immune response was evaluated. Cells isolated from gut associated lymphoid tissue (GALT) and from peripheral blood mononuclear cells (PBMC) of naive rats were used. The proliferation and cytokine production of these immune cells in the presence of the three isogenic Bifidobacterium animalis subsp. lactis strains (A1, A1dOx and A1dOxR), as well as their purified polymers, were in vitro analysed. The cytokine pattern produced by immune cells isolated from GALT showed that most levels remained stable in the presence of the three strains or their corresponding polymers. However, in PBMC the UV-inactivated bacteria induced higher levels of the ratios IFN gamma/IL-17, TNF alpha/IL-10 and TNF alpha/TGF beta, and no variation in the ratio IFN gamma/IL-4. Thus, B. animalis subsp. lactis strains were able to activate blood monocytes as well as T lymphocytes towards a mild inflammatory Th1 response. Furthermore, only the EPS-A1dOxR was able to stimulate a response in a similar way than its EPS-producing bacterium. Our work supports the notion that some bifidobacterial EPS could play a role in mediating the dialog of these microorganisms with the immune system. In addition, this study emphasizes the effect that the origin of the immune cells has in results obtained; this could explain the great amount of contradiction found in literature about the immunomodulation capability of EPS from probiotic bacteria. PB - Elsevier Sci Ltd, Oxford T2 - Anaerobe T1 - Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue EP - 30 SP - 24 VL - 26 DO - 10.1016/j.anaerobe.2014.01.003 ER -
@article{ author = "Hidalgo-Cantabrana, Claudio and Živković, Milica and Lopez, Patricia and Suarez, Ana and Miljković, Marija and Kojić, Milan and Margolles, Abelardo and Golić, Nataša and Ruas-Madiedo, Patricia", year = "2014", abstract = "The effect of exopolysaccharide (EPS) producing bifidobacteria, and the EPS derived thereof, on the modulation of immune response was evaluated. Cells isolated from gut associated lymphoid tissue (GALT) and from peripheral blood mononuclear cells (PBMC) of naive rats were used. The proliferation and cytokine production of these immune cells in the presence of the three isogenic Bifidobacterium animalis subsp. lactis strains (A1, A1dOx and A1dOxR), as well as their purified polymers, were in vitro analysed. The cytokine pattern produced by immune cells isolated from GALT showed that most levels remained stable in the presence of the three strains or their corresponding polymers. However, in PBMC the UV-inactivated bacteria induced higher levels of the ratios IFN gamma/IL-17, TNF alpha/IL-10 and TNF alpha/TGF beta, and no variation in the ratio IFN gamma/IL-4. Thus, B. animalis subsp. lactis strains were able to activate blood monocytes as well as T lymphocytes towards a mild inflammatory Th1 response. Furthermore, only the EPS-A1dOxR was able to stimulate a response in a similar way than its EPS-producing bacterium. Our work supports the notion that some bifidobacterial EPS could play a role in mediating the dialog of these microorganisms with the immune system. In addition, this study emphasizes the effect that the origin of the immune cells has in results obtained; this could explain the great amount of contradiction found in literature about the immunomodulation capability of EPS from probiotic bacteria.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Anaerobe", title = "Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue", pages = "30-24", volume = "26", doi = "10.1016/j.anaerobe.2014.01.003" }
Hidalgo-Cantabrana, C., Živković, M., Lopez, P., Suarez, A., Miljković, M., Kojić, M., Margolles, A., Golić, N.,& Ruas-Madiedo, P.. (2014). Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue. in Anaerobe Elsevier Sci Ltd, Oxford., 26, 24-30. https://doi.org/10.1016/j.anaerobe.2014.01.003
Hidalgo-Cantabrana C, Živković M, Lopez P, Suarez A, Miljković M, Kojić M, Margolles A, Golić N, Ruas-Madiedo P. Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue. in Anaerobe. 2014;26:24-30. doi:10.1016/j.anaerobe.2014.01.003 .
Hidalgo-Cantabrana, Claudio, Živković, Milica, Lopez, Patricia, Suarez, Ana, Miljković, Marija, Kojić, Milan, Margolles, Abelardo, Golić, Nataša, Ruas-Madiedo, Patricia, "Exopolysaccharide-producing Bifidobacterium animalis subsp lactis strains and their polymers elicit different responses on immune cells from blood and gut associated lymphoid tissue" in Anaerobe, 26 (2014):24-30, https://doi.org/10.1016/j.anaerobe.2014.01.003 . .