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Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+lymphocytes into IL-17/GM-CSF-producing cells in vitro
dc.creator | Stojić-Vukanić, Zorica | |
dc.creator | Bufan, Biljana | |
dc.creator | Pilipović, Ivan | |
dc.creator | Vujnović, Ivana | |
dc.creator | Nacka-Aleksić, Mirjana | |
dc.creator | Petrović, Raisa | |
dc.creator | Arsenović-Ranin, Nevena | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2021-02-18T10:46:34Z | |
dc.date.available | 2021-02-18T10:46:34Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1567-5769 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/460 | |
dc.description.abstract | There are little data on modulatory effects of estrogens on rat dendritic cell (DC) responses to inflammatory stimuli, and consequently their ability to activate and polarize CD4+ T lymphocyte-mediated immune responses. Splenic conventional DCs from young female Albino Oxford rats were activated in vitro with LPS (TLR4 agonist) or R848 (TLR7/8 agonist) in the presence and absence of 17 beta-estradiol (E2), and their allostimulatory and CD4+ lymphocyte polarizing ability in mixed leukocyte culture (MLC) were studied. Irrespective of the E2 presence, LPS and R848 up-regulated the expression of MHC II on DCs, so they exhibited enhanced allostimulatory capacity in co-culture with CD4+ lymphocytes. On the other hand, E2 promoted stimulatory action of both TLRs on OX62+ DC IL-23 production, augmented their stimulatory effects on IL-6 and IL-1 beta production, but diminished their enhancing effects on the expression IL-10 and IL-27 by DCs. Consequently, in MLC, OX62+ DCs activated/matured in the co-presence of E2 and either LPS or R848 increased the levels of IL-17, the signature Th17 cell cytoldne, when compared with those activated/matured in the absence of E2. GM-CSF levels were also increased in these MLC. Given that the expression of IL-7 mRNA was diminished in DCs activated/matured in the co presence of E2 and TLR, this increase most likely did not reflect enhanced differentiation of Th cells producing GM-CSF only (Th-GM). Conclusions: E2 augments capacity of LPS- and R848-activated/matured DCs from young rat spleen to induce differentiation of IL-17- and GM-CSF-producing cells. (C) 2016 Elsevier B.V. All rights reserved. | en |
dc.publisher | Elsevier, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS// | |
dc.rights | restrictedAccess | |
dc.source | International Immunopharmacology | |
dc.subject | Conventional dendritic cell polarizing ability | en |
dc.subject | Estradiol | en |
dc.subject | LPS | en |
dc.subject | Mixed leukocyte culture | en |
dc.subject | R848 | en |
dc.title | Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+lymphocytes into IL-17/GM-CSF-producing cells in vitro | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 253 | |
dc.citation.other | 40: 244-253 | |
dc.citation.rank | M22 | |
dc.citation.spage | 244 | |
dc.citation.volume | 40 | |
dc.identifier.doi | 10.1016/j.intimp.2016.09.001 | |
dc.identifier.pmid | 27620506 | |
dc.identifier.scopus | 2-s2.0-84986321381 | |
dc.identifier.wos | 000389087500031 | |
dc.type.version | publishedVersion |