Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese
Само за регистроване кориснике
2006
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains produced two additional bacteriocins (LsbA and LsbB) with genes responsible for their production and immunity located on the small rolling circle-replicating plasmid pMN5. Using deletion experiments of pMN5, minimal replicon of the plasmid and involvement of a bacteriocin locus in plasmid maintenance were identified. In addition, plasmid curing experiments showed that genes for catabolism or tran...sport of 10 carbohydrates in the strain BGMN1-5 were plasmid located.
Кључне речи:
plasmid curing / natural isolates / lactococci / bacteriocinИзвор:
Canadian Journal of Microbiology, 2006, 52, 11, 1110-1120Издавач:
- Canadian Science Publishing, Ottawa
DOI: 10.1139/W06-072
ISSN: 0008-4166
PubMed: 17215903
WoS: 000243822100012
Scopus: 2-s2.0-33846963757
Институција/група
TorlakTY - JOUR AU - Kojić, Milan AU - Strahinić, Ivana AU - Fira, Đorđe AU - Jovčić, Branko AU - Topisirović, Ljubiša PY - 2006 UR - http://intor.torlakinstitut.com/handle/123456789/810 AB - In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains produced two additional bacteriocins (LsbA and LsbB) with genes responsible for their production and immunity located on the small rolling circle-replicating plasmid pMN5. Using deletion experiments of pMN5, minimal replicon of the plasmid and involvement of a bacteriocin locus in plasmid maintenance were identified. In addition, plasmid curing experiments showed that genes for catabolism or transport of 10 carbohydrates in the strain BGMN1-5 were plasmid located. PB - Canadian Science Publishing, Ottawa T2 - Canadian Journal of Microbiology T1 - Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese EP - 1120 IS - 11 SP - 1110 VL - 52 DO - 10.1139/W06-072 ER -
@article{ author = "Kojić, Milan and Strahinić, Ivana and Fira, Đorđe and Jovčić, Branko and Topisirović, Ljubiša", year = "2006", abstract = "In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains produced two additional bacteriocins (LsbA and LsbB) with genes responsible for their production and immunity located on the small rolling circle-replicating plasmid pMN5. Using deletion experiments of pMN5, minimal replicon of the plasmid and involvement of a bacteriocin locus in plasmid maintenance were identified. In addition, plasmid curing experiments showed that genes for catabolism or transport of 10 carbohydrates in the strain BGMN1-5 were plasmid located.", publisher = "Canadian Science Publishing, Ottawa", journal = "Canadian Journal of Microbiology", title = "Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese", pages = "1120-1110", number = "11", volume = "52", doi = "10.1139/W06-072" }
Kojić, M., Strahinić, I., Fira, Đ., Jovčić, B.,& Topisirović, L.. (2006). Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese. in Canadian Journal of Microbiology Canadian Science Publishing, Ottawa., 52(11), 1110-1120. https://doi.org/10.1139/W06-072
Kojić M, Strahinić I, Fira Đ, Jovčić B, Topisirović L. Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese. in Canadian Journal of Microbiology. 2006;52(11):1110-1120. doi:10.1139/W06-072 .
Kojić, Milan, Strahinić, Ivana, Fira, Đorđe, Jovčić, Branko, Topisirović, Ljubiša, "Plasmid content and bacteriocin production by five strains of Lactococcus lactis isolated from semi-hard homemade cheese" in Canadian Journal of Microbiology, 52, no. 11 (2006):1110-1120, https://doi.org/10.1139/W06-072 . .