The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The Bacillus pumilus gene encoding acetyl xylan esterase tare) was identified and characterized. The axe gene was expressed and the recombinant enzyme produced in Escherichia coli was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) from B. pumilus. The AXE primary structure was 76% identical to the cephalosporin C deacetylase of B. subtilis, and 40% to two recently identified AXEs from Thermoanaerobacterium and Thermotoga maritima. These four proteins are of similar sire and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.
Кључне речи:
cephalosporin C deacetylase / Bacillus pumilus / acetyl xylan esteraseИзвор:
Microbiology-Sgm, 2000, 146, 1585-1591Издавач:
- Microbiology Soc, London
DOI: 10.1099/00221287-146-7-1585
ISSN: 1350-0872
PubMed: 10878123
WoS: 000088174000010
Scopus: 2-s2.0-0033910674
Институција/група
TorlakTY - JOUR AU - Degrassi, G AU - Kojić, Milan AU - Ljubijankić, G AU - Venturi, V PY - 2000 UR - http://intor.torlakinstitut.com/handle/123456789/791 AB - The Bacillus pumilus gene encoding acetyl xylan esterase tare) was identified and characterized. The axe gene was expressed and the recombinant enzyme produced in Escherichia coli was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) from B. pumilus. The AXE primary structure was 76% identical to the cephalosporin C deacetylase of B. subtilis, and 40% to two recently identified AXEs from Thermoanaerobacterium and Thermotoga maritima. These four proteins are of similar sire and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C. PB - Microbiology Soc, London T2 - Microbiology-Sgm T1 - The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity EP - 1591 SP - 1585 VL - 146 DO - 10.1099/00221287-146-7-1585 ER -
@article{ author = "Degrassi, G and Kojić, Milan and Ljubijankić, G and Venturi, V", year = "2000", abstract = "The Bacillus pumilus gene encoding acetyl xylan esterase tare) was identified and characterized. The axe gene was expressed and the recombinant enzyme produced in Escherichia coli was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) from B. pumilus. The AXE primary structure was 76% identical to the cephalosporin C deacetylase of B. subtilis, and 40% to two recently identified AXEs from Thermoanaerobacterium and Thermotoga maritima. These four proteins are of similar sire and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.", publisher = "Microbiology Soc, London", journal = "Microbiology-Sgm", title = "The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity", pages = "1591-1585", volume = "146", doi = "10.1099/00221287-146-7-1585" }
Degrassi, G., Kojić, M., Ljubijankić, G.,& Venturi, V.. (2000). The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity. in Microbiology-Sgm Microbiology Soc, London., 146, 1585-1591. https://doi.org/10.1099/00221287-146-7-1585
Degrassi G, Kojić M, Ljubijankić G, Venturi V. The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity. in Microbiology-Sgm. 2000;146:1585-1591. doi:10.1099/00221287-146-7-1585 .
Degrassi, G, Kojić, Milan, Ljubijankić, G, Venturi, V, "The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity" in Microbiology-Sgm, 146 (2000):1585-1591, https://doi.org/10.1099/00221287-146-7-1585 . .