Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9
Аутори
Tolinački, MajaKojić, Milan
Lozo, Jelena
Terzić-Vidojević, Amarela
Topisirović, Ljubiša
Fira, Đorđe
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seeme...d to be responsible for both bacteriocin BacUB9 production and host immunity.
Кључне речи:
Lactobacillus / bacteriocin / antimicrobial activityИзвор:
Archives of Biological Sciences, 2010, 62, 4, 889-899Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Изучавање регулације експресије гена одабраних индустријских микроорганизама (RS-MESTD-MPN2006-2010-143036)
DOI: 10.2298/ABS1004889T
ISSN: 0354-4664
WoS: 000287217500004
Scopus: 2-s2.0-78650987452
Институција/група
TorlakTY - JOUR AU - Tolinački, Maja AU - Kojić, Milan AU - Lozo, Jelena AU - Terzić-Vidojević, Amarela AU - Topisirović, Ljubiša AU - Fira, Đorđe PY - 2010 UR - http://intor.torlakinstitut.com/handle/123456789/719 AB - The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9 EP - 899 IS - 4 SP - 889 VL - 62 DO - 10.2298/ABS1004889T ER -
@article{ author = "Tolinački, Maja and Kojić, Milan and Lozo, Jelena and Terzić-Vidojević, Amarela and Topisirović, Ljubiša and Fira, Đorđe", year = "2010", abstract = "The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9", pages = "899-889", number = "4", volume = "62", doi = "10.2298/ABS1004889T" }
Tolinački, M., Kojić, M., Lozo, J., Terzić-Vidojević, A., Topisirović, L.,& Fira, Đ.. (2010). Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 62(4), 889-899. https://doi.org/10.2298/ABS1004889T
Tolinački M, Kojić M, Lozo J, Terzić-Vidojević A, Topisirović L, Fira Đ. Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences. 2010;62(4):889-899. doi:10.2298/ABS1004889T .
Tolinački, Maja, Kojić, Milan, Lozo, Jelena, Terzić-Vidojević, Amarela, Topisirović, Ljubiša, Fira, Đorđe, "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9" in Archives of Biological Sciences, 62, no. 4 (2010):889-899, https://doi.org/10.2298/ABS1004889T . .