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dc.creatorNikodijević, Slavomir
dc.creatorBlagojević, Veljko
dc.creatorĆuruvija, Ivana
dc.creatorKosanović, Dejana
dc.creatorĐukić, Tamara
dc.creatorĐorđević, Brižita
dc.creatorIlić, Vesna
dc.creatorMinić, Rajna
dc.date.accessioned2022-12-05T07:14:09Z
dc.date.available2022-12-05T07:14:09Z
dc.date.issued2022
dc.identifier.issn0300-9475
dc.identifier.issn1365-3083 (eISSN)
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/632
dc.description.abstractIncreased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r = .70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r = .86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r = .88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.sr
dc.language.isoensr
dc.publisherThe Scandinavian Foundation for Immunologysr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200177/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceScandinavian Journal of Immunologysr
dc.subjectIgAsr
dc.subjectIgA subclassessr
dc.subjectMicroorganismssr
dc.subjectsalivary IgAsr
dc.subjectserum IgAsr
dc.titleSelectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humanssr
dc.typearticlesr
dc.rights.licenseARRsr
dc.citation.rankM23
dc.citation.volume96
dc.identifier.doi10.1111/sji.13223
dc.identifier.scopus2-s2.0-85139909517
dc.type.versionpublishedVersionsr


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Приказ основних података о документу