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dc.creatorDragačević, Luka
dc.creatorLopandić, Zorana
dc.creatorGavrović-Jankulović, Marija
dc.creatorŽivković, Irena
dc.creatorBlagojević, Veljko
dc.creatorPolović, Natalija
dc.creatorMinić, Rajna
dc.date.accessioned2022-05-17T06:29:58Z
dc.date.available2022-05-17T06:29:58Z
dc.date.issued2022
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291 (eISSN)
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/621
dc.description.abstractThe surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.sr
dc.language.isoensr
dc.publisherSpringersr
dc.relationMinistry of Education, Science and Technological Development of the Republic of Serbia, Grant 451-03-9/2021-14sr
dc.relation.isreferencedbyhttps://hdl.handle.net/21.15107/rcub_intor_642
dc.rightsrestrictedAccesssr
dc.sourceApplied Biochemistry and Biotechnologysr
dc.subjectGlycosylationsr
dc.subjectPlant lectinssr
dc.subjectMicroorganismssr
dc.subjectYeastssr
dc.subjectSalmonella Lactobacillussr
dc.titleComparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycanssr
dc.typearticlesr
dc.rights.licenseARRsr
dc.citation.epage2060
dc.citation.rankM22
dc.citation.spage2047
dc.citation.volume194
dc.description.otherSupplementary information: [https://hdl.handle.net/21.15107/rcub_intor_642]
dc.identifier.doi10.1007/s12010-021-03772-w
dc.identifier.scopus2-s2.0-85122876661
dc.identifier.wos000741601700005
dc.type.versionpublishedVersionsr


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Приказ основних података о документу