InTOR - Repository of the Institute “Torlak”
Institute of Virology, Vaccines and Sera “Torlak”
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   InTOR
  • Torlak
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report

Thumbnail
2021
s12941-021-00423-0.pdf (1.543Mb)
Authors
Janjusevic, Ana
Markovic Denic, Ljiljana
Minic, Rajna
Anita, Grgurevic
Cirkovic, Ivana
Article (Published version)
Metadata
Show full item record
Abstract
Background: The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology of VRE is insufficiently investigated, yet it could be of key importance in infection control. The aim of this study was to provide baseline data on VRE intestinal carriage among high-risk patients in Serbian university hospitals, to determine the phenotypic/genotypic profiles of the isolated VRE, to obtain knowledge of local resistance patterns and bridge the gaps in current VRE surveillance. Methods: The VRE reservoir was investigated using stool samples from 268 inpatients. Characterization of isolated VRE stains consisted of BD Phoenix system, genotypic identification, glycopeptide and quinupristin-dalfopristin (Q-D) resistance probing, virulence gene (esp, hyl, efaA, asa1, gelE, cpd) detection and MLVA. Biofilm formation was evaluated by the microtiter plate method. Results: VRE carriage prevalence among at...-risk patients was 28.7%. All VRE strains were vanA positive multidrug-resistant Enterococcus faecium (VRfm), harboring ermB-1 (38.9%), esp (84%), efaA (71.2%), hyl (54.5%), asa1 (23.4%), gelE and cpd (11.6%) each. Ability of biofilm production was detected in 20.8%. Genetic relatedness of the isolates revealed 13 clusters, heterogeneous picture and 25 unique MTs profiles. Conclusion: The obtained prevalence of VRE intestinal carriage among high-risk inpatients in Serbia is higher than the European average, with high percentage of multidrug resistance. The emergence of resistance to Q-D is of particular concern. Close monitoring of pattern of resistance and strict adherence to specific guidelines are urgently needed in Serbia.

Keywords:
Antibiotic susceptibility / MLVA sreening / Serbia / VRE carriage
Source:
Annals of Clinical Microbiology and Antimicrobials, 2021, 20, 18-
Publisher:
  • BMC
Funding / projects:
  • Clinical and epidemiological study of adverse events in hospital patients (RS-175046)
  • Antibiotic resistant bacterial pathogens in Serbia: phenotypic and genotypic characterization (RS-175039)

DOI: 10.1186/s12941-021-00423-0

ISSN: 1476-0711

WoS: 000631141000001

Scopus: 2-s2.0-85103231168
[ Google Scholar ]
2
URI
http://intor.torlakinstitut.com/handle/123456789/614
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Janjusevic, Ana
AU  - Markovic Denic, Ljiljana
AU  - Minic, Rajna
AU  - Anita, Grgurevic
AU  - Cirkovic, Ivana
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/614
AB  - Background: The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology of VRE is insufficiently investigated, yet it could be of key importance in infection control. The aim of this study was to provide baseline data on VRE intestinal carriage among high-risk patients in Serbian university hospitals, to determine the phenotypic/genotypic profiles of the isolated VRE, to obtain knowledge of local resistance patterns and bridge the gaps in current VRE surveillance.

Methods: The VRE reservoir was investigated using stool samples from 268 inpatients. Characterization of isolated VRE stains consisted of BD Phoenix system, genotypic identification, glycopeptide and quinupristin-dalfopristin (Q-D) resistance probing, virulence gene (esp, hyl, efaA, asa1, gelE, cpd) detection and MLVA. Biofilm formation was evaluated by the microtiter plate method.

Results: VRE carriage prevalence among at-risk patients was 28.7%. All VRE strains were vanA positive multidrug-resistant Enterococcus faecium (VRfm), harboring ermB-1 (38.9%), esp (84%), efaA (71.2%), hyl (54.5%), asa1 (23.4%), gelE and cpd (11.6%) each. Ability of biofilm production was detected in 20.8%. Genetic relatedness of the isolates revealed 13 clusters, heterogeneous picture and 25 unique MTs profiles.

Conclusion: The obtained prevalence of VRE intestinal carriage among high-risk inpatients in Serbia is higher than the European average, with high percentage of multidrug resistance. The emergence of resistance to Q-D is of particular concern. Close monitoring of pattern of resistance and strict adherence to specific guidelines are urgently needed in Serbia.
PB  - BMC
T2  - Annals of Clinical Microbiology and Antimicrobials
T1  - Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report
SP  - 18
VL  - 20
DO  - 10.1186/s12941-021-00423-0
ER  - 
@article{
author = "Janjusevic, Ana and Markovic Denic, Ljiljana and Minic, Rajna and Anita, Grgurevic and Cirkovic, Ivana",
year = "2021",
abstract = "Background: The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology of VRE is insufficiently investigated, yet it could be of key importance in infection control. The aim of this study was to provide baseline data on VRE intestinal carriage among high-risk patients in Serbian university hospitals, to determine the phenotypic/genotypic profiles of the isolated VRE, to obtain knowledge of local resistance patterns and bridge the gaps in current VRE surveillance.

Methods: The VRE reservoir was investigated using stool samples from 268 inpatients. Characterization of isolated VRE stains consisted of BD Phoenix system, genotypic identification, glycopeptide and quinupristin-dalfopristin (Q-D) resistance probing, virulence gene (esp, hyl, efaA, asa1, gelE, cpd) detection and MLVA. Biofilm formation was evaluated by the microtiter plate method.

Results: VRE carriage prevalence among at-risk patients was 28.7%. All VRE strains were vanA positive multidrug-resistant Enterococcus faecium (VRfm), harboring ermB-1 (38.9%), esp (84%), efaA (71.2%), hyl (54.5%), asa1 (23.4%), gelE and cpd (11.6%) each. Ability of biofilm production was detected in 20.8%. Genetic relatedness of the isolates revealed 13 clusters, heterogeneous picture and 25 unique MTs profiles.

Conclusion: The obtained prevalence of VRE intestinal carriage among high-risk inpatients in Serbia is higher than the European average, with high percentage of multidrug resistance. The emergence of resistance to Q-D is of particular concern. Close monitoring of pattern of resistance and strict adherence to specific guidelines are urgently needed in Serbia.",
publisher = "BMC",
journal = "Annals of Clinical Microbiology and Antimicrobials",
title = "Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report",
pages = "18",
volume = "20",
doi = "10.1186/s12941-021-00423-0"
}
Janjusevic, A., Markovic Denic, L., Minic, R., Anita, G.,& Cirkovic, I.. (2021). Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report. in Annals of Clinical Microbiology and Antimicrobials
BMC., 20, 18.
https://doi.org/10.1186/s12941-021-00423-0
Janjusevic A, Markovic Denic L, Minic R, Anita G, Cirkovic I. Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report. in Annals of Clinical Microbiology and Antimicrobials. 2021;20:18.
doi:10.1186/s12941-021-00423-0 .
Janjusevic, Ana, Markovic Denic, Ljiljana, Minic, Rajna, Anita, Grgurevic, Cirkovic, Ivana, "Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report" in Annals of Clinical Microbiology and Antimicrobials, 20 (2021):18,
https://doi.org/10.1186/s12941-021-00423-0 . .

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About InTOR | Send Feedback

OpenAIRERCUB