Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter
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2020
Authors
Michalickova, DanicaMinić, Rajna
Kotur-Stevuljević, Jelena
Anđelković, Marija
Dikić, Nenad
Kostić-Vučićević, Marija
Slanar, Ondrej
Đorđević, Brižita
Article (Published version)
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Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, M, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter.J Strength Cond Res34(10): 2965-2973, 2020-The current study monitored markers of immunological and oxidative status in 9 male elite endurance athletes: V?o(2)max: 68 +/- 11 ml center dot kg(-1)center dot min(-1), age: 24 +/- 2.5 years, and training loads: 128 +/- 21 metabolic equivalents-h center dot wk(-1)during a 3-month preparation period in winter (January-March). Self-rated state of moods evaluation (by Profile of Mood States questionnaire) was performed, and blood samples were collected at the beginning and end of the study. Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. The level of concanavalin A (ConA)-stimulated interferon-gamma (IFN-gamma) from peripheral blood mononuc...lear cells (PBMCs) was increased (562 [147-852] vs. 1,097 [451-1842] pg center dot ml(-1),p= 0.013). Also, the level of transforming growth factor-1 (TGF-beta 1) in serum was elevated (2.5 [1.4-5.1] vs. 7.2 [4.9-8.2] ng center dot ml(-1),p= 0.015). There was no change in the level of peptidoglycan (PGN)-stimulated interleukin (IL)-10 from PBMCs. There were no significant changes in PBMCs proliferation/viability on stimulation with ConA and PGN during the study. No changes in superoxide dismutase, prooxidative-antioxidative balance, total oxidant status (TOS), and thiobarbituric acid reactive substances were observed along the study. Total antioxidant status (TAS) was increased (910 +/- 174 vs. 1,090 +/- 102 mu mol center dot L-1,p= 0.018), and activity of paraoxonase (PON1) was decreased (523 +/- 295 vs. 335 +/- 183 U center dot L-1,p= 0.003) at the end of the study. Advanced oxidation protein products were increased (25 +/- 7.9 vs. 42 +/- 7.6 mu mol center dot L-1,p= 0.011). The self-rated sense of vigor significantly declined (20 +/- 2.1 vs. 14 +/- 3.4,p= 0.045). In conclusion, 3 months of regular training in winter induced prominent changes in cytokines, biomarkers of oxidative stress, and antioxidative enzyme activity. These changes might increase susceptibility of athletes to disease and muscle damage and consequently lead to performance reduction.
Keywords:
cytokines / salivary IgA antibodies / POMSSource:
Journal of Strength and Conditioning Research, 2020, 34, 10, 2965-2973Publisher:
- Lippincott Williams & Wilkins, Philadelphia
Funding / projects:
- Charles University Project Progress Q25
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-46009)
- Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
DOI: 10.1519/JSC.0000000000002780
ISSN: 1064-8011
PubMed: 30199454
WoS: 000575992200033
Scopus: 2-s2.0-85071996741
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Institution/Community
TorlakTY - JOUR AU - Michalickova, Danica AU - Minić, Rajna AU - Kotur-Stevuljević, Jelena AU - Anđelković, Marija AU - Dikić, Nenad AU - Kostić-Vučićević, Marija AU - Slanar, Ondrej AU - Đorđević, Brižita PY - 2020 UR - http://intor.torlakinstitut.com/handle/123456789/545 AB - Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, M, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter.J Strength Cond Res34(10): 2965-2973, 2020-The current study monitored markers of immunological and oxidative status in 9 male elite endurance athletes: V?o(2)max: 68 +/- 11 ml center dot kg(-1)center dot min(-1), age: 24 +/- 2.5 years, and training loads: 128 +/- 21 metabolic equivalents-h center dot wk(-1)during a 3-month preparation period in winter (January-March). Self-rated state of moods evaluation (by Profile of Mood States questionnaire) was performed, and blood samples were collected at the beginning and end of the study. Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. The level of concanavalin A (ConA)-stimulated interferon-gamma (IFN-gamma) from peripheral blood mononuclear cells (PBMCs) was increased (562 [147-852] vs. 1,097 [451-1842] pg center dot ml(-1),p= 0.013). Also, the level of transforming growth factor-1 (TGF-beta 1) in serum was elevated (2.5 [1.4-5.1] vs. 7.2 [4.9-8.2] ng center dot ml(-1),p= 0.015). There was no change in the level of peptidoglycan (PGN)-stimulated interleukin (IL)-10 from PBMCs. There were no significant changes in PBMCs proliferation/viability on stimulation with ConA and PGN during the study. No changes in superoxide dismutase, prooxidative-antioxidative balance, total oxidant status (TOS), and thiobarbituric acid reactive substances were observed along the study. Total antioxidant status (TAS) was increased (910 +/- 174 vs. 1,090 +/- 102 mu mol center dot L-1,p= 0.018), and activity of paraoxonase (PON1) was decreased (523 +/- 295 vs. 335 +/- 183 U center dot L-1,p= 0.003) at the end of the study. Advanced oxidation protein products were increased (25 +/- 7.9 vs. 42 +/- 7.6 mu mol center dot L-1,p= 0.011). The self-rated sense of vigor significantly declined (20 +/- 2.1 vs. 14 +/- 3.4,p= 0.045). In conclusion, 3 months of regular training in winter induced prominent changes in cytokines, biomarkers of oxidative stress, and antioxidative enzyme activity. These changes might increase susceptibility of athletes to disease and muscle damage and consequently lead to performance reduction. PB - Lippincott Williams & Wilkins, Philadelphia T2 - Journal of Strength and Conditioning Research T1 - Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter EP - 2973 IS - 10 SP - 2965 VL - 34 DO - 10.1519/JSC.0000000000002780 ER -
@article{ author = "Michalickova, Danica and Minić, Rajna and Kotur-Stevuljević, Jelena and Anđelković, Marija and Dikić, Nenad and Kostić-Vučićević, Marija and Slanar, Ondrej and Đorđević, Brižita", year = "2020", abstract = "Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, M, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter.J Strength Cond Res34(10): 2965-2973, 2020-The current study monitored markers of immunological and oxidative status in 9 male elite endurance athletes: V?o(2)max: 68 +/- 11 ml center dot kg(-1)center dot min(-1), age: 24 +/- 2.5 years, and training loads: 128 +/- 21 metabolic equivalents-h center dot wk(-1)during a 3-month preparation period in winter (January-March). Self-rated state of moods evaluation (by Profile of Mood States questionnaire) was performed, and blood samples were collected at the beginning and end of the study. Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. The level of concanavalin A (ConA)-stimulated interferon-gamma (IFN-gamma) from peripheral blood mononuclear cells (PBMCs) was increased (562 [147-852] vs. 1,097 [451-1842] pg center dot ml(-1),p= 0.013). Also, the level of transforming growth factor-1 (TGF-beta 1) in serum was elevated (2.5 [1.4-5.1] vs. 7.2 [4.9-8.2] ng center dot ml(-1),p= 0.015). There was no change in the level of peptidoglycan (PGN)-stimulated interleukin (IL)-10 from PBMCs. There were no significant changes in PBMCs proliferation/viability on stimulation with ConA and PGN during the study. No changes in superoxide dismutase, prooxidative-antioxidative balance, total oxidant status (TOS), and thiobarbituric acid reactive substances were observed along the study. Total antioxidant status (TAS) was increased (910 +/- 174 vs. 1,090 +/- 102 mu mol center dot L-1,p= 0.018), and activity of paraoxonase (PON1) was decreased (523 +/- 295 vs. 335 +/- 183 U center dot L-1,p= 0.003) at the end of the study. Advanced oxidation protein products were increased (25 +/- 7.9 vs. 42 +/- 7.6 mu mol center dot L-1,p= 0.011). The self-rated sense of vigor significantly declined (20 +/- 2.1 vs. 14 +/- 3.4,p= 0.045). In conclusion, 3 months of regular training in winter induced prominent changes in cytokines, biomarkers of oxidative stress, and antioxidative enzyme activity. These changes might increase susceptibility of athletes to disease and muscle damage and consequently lead to performance reduction.", publisher = "Lippincott Williams & Wilkins, Philadelphia", journal = "Journal of Strength and Conditioning Research", title = "Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter", pages = "2973-2965", number = "10", volume = "34", doi = "10.1519/JSC.0000000000002780" }
Michalickova, D., Minić, R., Kotur-Stevuljević, J., Anđelković, M., Dikić, N., Kostić-Vučićević, M., Slanar, O.,& Đorđević, B.. (2020). Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter. in Journal of Strength and Conditioning Research Lippincott Williams & Wilkins, Philadelphia., 34(10), 2965-2973. https://doi.org/10.1519/JSC.0000000000002780
Michalickova D, Minić R, Kotur-Stevuljević J, Anđelković M, Dikić N, Kostić-Vučićević M, Slanar O, Đorđević B. Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter. in Journal of Strength and Conditioning Research. 2020;34(10):2965-2973. doi:10.1519/JSC.0000000000002780 .
Michalickova, Danica, Minić, Rajna, Kotur-Stevuljević, Jelena, Anđelković, Marija, Dikić, Nenad, Kostić-Vučićević, Marija, Slanar, Ondrej, Đorđević, Brižita, "Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter" in Journal of Strength and Conditioning Research, 34, no. 10 (2020):2965-2973, https://doi.org/10.1519/JSC.0000000000002780 . .