Influence of aging on germinal centre reaction and antibody response to inactivated influenza virus antigens in mice: sex-based differences
Само за регистроване кориснике
2019
Аутори
Arsenović-Ranin, Nevena
Petrović, Raisa
Živković, Irena
Bufan, Biljana

Stoiljković, Vera

Leposavić, Gordana

Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
The study examined sex-specificities in age-related changes in BALB/c mice IgG antibody responses to immunisation with trivalent inactivated split-virus influenza bulk. Aging diminished the total serum IgG antibody responses to H1N1 and H3N2 and B influenza virus antigens in mice of both sexes, but they remained greater in aged females. This sex difference in aged mice correlated with the greater post-immunisation increase in the frequency of spleen germinal centre (GC) B cells and more favourable T follicular regulatory (Tfr)/GC B cell ratio, as Tfr cells are suggested to control antibody production through suppression of glycolysis. The greater post-immunisation GC B cell response in aged females compared with males correlated with the greater proliferation of B cells and CD4+ cells in splenocyte cultures from aged females restimulated with inactivated split-virus influenza from the bulk. To support the greater post-immunisation increase in the frequency GC B cell in aged females was... more favourable Tfr/T follicular helper (Tfh) cell ratio. Additionally, compared with aged males, in age-matched females the greater avidity of serum IgG antibodies was found. However, in aged females IgG2a/IgG1 antibody ratio, reflecting spleen Th1/Th2 cytokine balance, was shifted towards IgG1 when compared with age-matched male mice. This shift was ascribed to a more prominent decline in the titres of functionally important IgG2a antibodies in females with aging. The study suggest that biological sex should be considered as a variable in designing strategies to manipulate with immune outcome of immunisation in aged animals, and possibly, at very long distance, humans.
Кључне речи:
Aging / Sex differences / Influenza vaccine / IgG antibody avidity / IgG2a / IgG1 ratio / Tfr / Tfh cell ratioИзвор:
Biogerontology, 2019, 20, 4, 475-496Издавач:
- Springer, New York
Финансирање / пројекти:
DOI: 10.1007/s10522-019-09811-8
ISSN: 1389-5729
PubMed: 31049769