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Noradrenaline modulates CD4+T cell priming in rat experimental autoimmune encephalomyelitis: a role for the alpha(1)-adrenoceptor

Само за регистроване кориснике
2019
Аутори
Pilipović, Ivan
Vujnović, Ivana
Stojić-Vukanić, Zorica
Petrović, Raisa
Kosec, Duško
Nacka-Aleksić, Mirjana
Jasnić, Nebojša
Leposavić, Gordana
Чланак у часопису (Објављена верзија)
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Апстракт
Pharmacological blockade of alpha(1)-adrenoceptor is shown to influence development of experimental autoimmune encephalomyelitis (EAE), an IL-17-producing CD4+TCR+ (Th17) cell-mediated disease mimicking multiple sclerosis. Considering significance of CD4+ cell priming for the clinical outcome of EAE, the study examined alpha(1)-adrenoceptor-mediated influence of catecholamines, particularly those derived from draining lymph node (dLN) cells (as catecholamine supply from nerve fibers decreases with the initiation of autoimmune diseases) for CD4+ cell priming. The results confirmed diminishing effect of immunization on nerve fiber-derived noradrenaline supply and showed that antigen presenting and CD4+ cells synthesize catecholamines, while antigen presenting cells and only CD4+CD25+Foxp3+ regulatory T cells (Tregs) express alpha(1)-adrenoceptor. The analysis of influence of alpha(1)-adrenoceptor antagonist prazosin on the myelin basic protein (MBP)-stimulated CD4+ lymphocytes in dLN cel...l culture showed their diminished proliferation in the presence of prazosin. This was consistent with prazosin enhancing effect on Treg frequency and their Foxp3 expression in these cultures. The latter was associated with upregulation of TGF-beta expression. Additionally, prazosin decreased antigen presenting cell activation and affected their cytokine profile by diminishing the frequency of cells that produce Th17 polarizing cytokines (IL-1 beta and IL-23) and increasing that of IL-10-producing cells. Consistently, the frequency of all IL-17A+ cells and those co-expressing GM-CSF within CD4+ lymphocytes was decreased in prazosin-supplemented MBP-stimulated dLN cell cultures. Collectively, the results indicated that dLN cell-derived catecholamines may influence EAE development by modulating interactions between distinct subtypes of CD4+ T cells and antigen presenting cells through alpha(1)-adrenoceptor and consequently CD4+ T cell priming.

Кључне речи:
Noradrenaline / alpha(1)-Adrenoceptor / EAE / Tregs / Th17 / CD4+lymphocyte proliferation
Извор:
Immunologic Research, 2019, 67, 2-3, 223-240
Издавач:
  • Humana Press Inc, Totowa
Финансирање / пројекти:
  • Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-175050)

DOI: 10.1007/s12026-019-09082-y

ISSN: 0257-277X

PubMed: 31396845

WoS: 000484525000008

Scopus: 2-s2.0-85070290853
[ Google Scholar ]
URI
http://intor.torlakinstitut.com/handle/123456789/531
Колекције
  • Radovi istraživača / Researchers’ publications
Институција/група
Torlak

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