Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo
Аутори
Inić-Kanada, AleksandraStein, Elisabeth
Stojanović, Marijana
Schuerer, Nadine
Ghasemian, Ehsan
Filipović, Ana
Marinković, Emilija
Kosanović, Dejana
Barisani-Asenbauer, Talin
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C red...uces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.
Кључне речи:
Carrageenan / Trachoma / Natural products / ChlamydiaИзвор:
Journal of Applied Phycology, 2018, 30, 4, 2601-2610Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Laura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-172049)
DOI: 10.1007/s10811-018-1435-0
ISSN: 0921-8971
PubMed: 30147240
WoS: 000440782100039
Scopus: 2-s2.0-85043697809
Институција/група
TorlakTY - JOUR AU - Inić-Kanada, Aleksandra AU - Stein, Elisabeth AU - Stojanović, Marijana AU - Schuerer, Nadine AU - Ghasemian, Ehsan AU - Filipović, Ana AU - Marinković, Emilija AU - Kosanović, Dejana AU - Barisani-Asenbauer, Talin PY - 2018 UR - http://intor.torlakinstitut.com/handle/123456789/502 AB - Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell. PB - Springer, Dordrecht T2 - Journal of Applied Phycology T1 - Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo EP - 2610 IS - 4 SP - 2601 VL - 30 DO - 10.1007/s10811-018-1435-0 ER -
@article{ author = "Inić-Kanada, Aleksandra and Stein, Elisabeth and Stojanović, Marijana and Schuerer, Nadine and Ghasemian, Ehsan and Filipović, Ana and Marinković, Emilija and Kosanović, Dejana and Barisani-Asenbauer, Talin", year = "2018", abstract = "Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.", publisher = "Springer, Dordrecht", journal = "Journal of Applied Phycology", title = "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo", pages = "2610-2601", number = "4", volume = "30", doi = "10.1007/s10811-018-1435-0" }
Inić-Kanada, A., Stein, E., Stojanović, M., Schuerer, N., Ghasemian, E., Filipović, A., Marinković, E., Kosanović, D.,& Barisani-Asenbauer, T.. (2018). Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology Springer, Dordrecht., 30(4), 2601-2610. https://doi.org/10.1007/s10811-018-1435-0
Inić-Kanada A, Stein E, Stojanović M, Schuerer N, Ghasemian E, Filipović A, Marinković E, Kosanović D, Barisani-Asenbauer T. Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology. 2018;30(4):2601-2610. doi:10.1007/s10811-018-1435-0 .
Inić-Kanada, Aleksandra, Stein, Elisabeth, Stojanović, Marijana, Schuerer, Nadine, Ghasemian, Ehsan, Filipović, Ana, Marinković, Emilija, Kosanović, Dejana, Barisani-Asenbauer, Talin, "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo" in Journal of Applied Phycology, 30, no. 4 (2018):2601-2610, https://doi.org/10.1007/s10811-018-1435-0 . .