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Mouse strain and sex as determinants of immune response to trivalent influenza vaccine
dc.creator | Petrović, Raisa | |
dc.creator | Bufan, Biljana | |
dc.creator | Arsenović-Ranin, Nevena | |
dc.creator | Živković, Irena | |
dc.creator | Minić, Rajna | |
dc.creator | Radojević, Katarina | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2021-02-18T10:49:20Z | |
dc.date.available | 2021-02-18T10:49:20Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0024-3205 | |
dc.identifier.uri | http://intor.torlakinstitut.com/handle/123456789/501 | |
dc.description.abstract | Aims: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). Main methods: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-gamma and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively. Key findings: BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sexmatched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-gamma than those from C57BL/6 mice. Significance: The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity. | en |
dc.publisher | Pergamon-Elsevier Science Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.rights | restrictedAccess | |
dc.source | Life Sciences | |
dc.subject | Mouse genetic background | en |
dc.subject | Influenza vaccine | en |
dc.subject | Antibody response | en |
dc.subject | Germinal center reaction | en |
dc.subject | Sex differences | en |
dc.title | Mouse strain and sex as determinants of immune response to trivalent influenza vaccine | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 126 | |
dc.citation.other | 207: 117-126 | |
dc.citation.rank | M21 | |
dc.citation.spage | 117 | |
dc.citation.volume | 207 | |
dc.identifier.doi | 10.1016/j.lfs.2018.05.056 | |
dc.identifier.pmid | 29859986 | |
dc.identifier.scopus | 2-s2.0-85048462717 | |
dc.identifier.wos | 000439876800015 | |
dc.type.version | publishedVersion |