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dc.creatorĆuruvija, Ivana
dc.creatorStanojević, Stanislava
dc.creatorArsenović-Ranin, Nevena
dc.creatorBlagojević, Veljko
dc.creatorDimitrijević, Mirjana
dc.creatorVidić-Danković, Biljana
dc.creatorVujić, Vesna
dc.date.accessioned2021-02-18T10:48:59Z
dc.date.available2021-02-18T10:48:59Z
dc.date.issued2017
dc.identifier.issn0360-3997
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/496
dc.description.abstractThe aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.en
dc.publisherSpringer/Plenum Publishers, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsrestrictedAccess
dc.sourceInflammation
dc.subjectestradiolen
dc.subjectestrogen receptor alpha (ER alpha)en
dc.subjectestrogen receptor beta (ER beta)en
dc.subjectmiddle-aged ratsen
dc.subjectthioglycollate-elicited peritoneal macrophagesen
dc.subjectsex differencesen
dc.titleSex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expressionen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1101
dc.citation.issue3
dc.citation.other40(3): 1087-1101
dc.citation.rankM22
dc.citation.spage1087
dc.citation.volume40
dc.identifier.doi10.1007/s10753-017-0551-3
dc.identifier.pmid28353029
dc.identifier.rcubconv_410
dc.identifier.scopus2-s2.0-85016127131
dc.identifier.wos000401264900034
dc.type.versionpublishedVersion


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