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dc.creatorMrkić, Ivan
dc.creatorMinić, Rajna
dc.creatorBulat, Tanja
dc.creatorAradska, Jana
dc.creatorAtanasković-Marković, Marina
dc.creatorDrakulić, Branko
dc.creatorGavrović-Jankulović, Marija
dc.date.accessioned2021-02-18T10:48:16Z
dc.date.available2021-02-18T10:48:16Z
dc.date.issued2017
dc.identifier.issn0268-2575
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/485
dc.description.abstractBACKGROUND: Group 1 and group 2 allergens from house dust mite are the major elicitors of respiratory allergic diseases and the main candidates for immunotherapy. RESULTS: The potential therapeutic role of a chimera composed of recombinant Der p 2 (D2) linked to Influenza A virus hemagglutinin 1 (H1) for intranasal application was created, expressed and tested in a mouse model. H1D2 and D2 were produced by genetic engineering in Escherichia coli and their primary structure was confirmed by mass fingerprint. Both antigens preserved IgE reactivity in immunoblot with serum from seven house dust mite allergic persons. Balb/c mice were sensitized with D2 allergen in alum and subsequently received H1D2 or D2, intranasally. The reduced levels of serum D2 specific IgE, together with the increased serum specific IgG and IgA were detected in both groups which received H1D2 and D2 intranasally. A higher level of effector CD4+CD25+ spleen lymphocytes was found only in the group of mice which received i.n. H1D2. CONCLUSION: H1D2 chimera can have therapeutic potential in Der p 2 allergic persons as dual vaccine which, beside protective allergen specific, can provide protective antibodies against Influenza A virus hemagglutinin 1. (C) 2016 Society of Chemical Industryen
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chemical Technology and Biotechnology
dc.subjectallergen-specific immunotherapyen
dc.subjectDer p 2en
dc.subjecthaemagglutinin 1en
dc.subjectinfluenza virusen
dc.subjecthouse dust mite allergyen
dc.titleModulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimeraen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1335
dc.citation.issue6
dc.citation.other92(6): 1328-1335
dc.citation.rankM22
dc.citation.spage1328
dc.citation.volume92
dc.identifier.doi10.1002/jctb.5127
dc.identifier.rcubconv_413
dc.identifier.scopus2-s2.0-85002657252
dc.identifier.wos000403025100022
dc.type.versionpublishedVersion


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