Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection
Само за регистроване кориснике
2016
Аутори
Belij-Rammerstorfer, SandraInić-Kanada, Aleksandra
Stojanović, Marijana
Marinković, Emilija
Lukić, Ivana
Stein, Elisabeth
Montanaro, Jacqueline
Bintner, Nora
Schuerer, Nadine
Ghasemian, Ehsan
Kundi, Michael
Barisani-Asenbauer, Talin
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The l...ower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Кључне речи:
Trachoma / Infectious dose / Chlamydia caviae / Guinea pig ocular animal modelИзвор:
Microbes and Infection, 2016, 18, 4, 254-262Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Laura Bassi Centers of Expertise (FFG) [822768] - Austrian Research Promotion Agency
DOI: 10.1016/j.micinf.2015.12.001
ISSN: 1286-4579
PubMed: 26706818
WoS: 000374920600005
Scopus: 2-s2.0-84955327760
Институција/група
TorlakTY - JOUR AU - Belij-Rammerstorfer, Sandra AU - Inić-Kanada, Aleksandra AU - Stojanović, Marijana AU - Marinković, Emilija AU - Lukić, Ivana AU - Stein, Elisabeth AU - Montanaro, Jacqueline AU - Bintner, Nora AU - Schuerer, Nadine AU - Ghasemian, Ehsan AU - Kundi, Michael AU - Barisani-Asenbauer, Talin PY - 2016 UR - http://intor.torlakinstitut.com/handle/123456789/470 AB - The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Microbes and Infection T1 - Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection EP - 262 IS - 4 SP - 254 VL - 18 DO - 10.1016/j.micinf.2015.12.001 ER -
@article{ author = "Belij-Rammerstorfer, Sandra and Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Lukić, Ivana and Stein, Elisabeth and Montanaro, Jacqueline and Bintner, Nora and Schuerer, Nadine and Ghasemian, Ehsan and Kundi, Michael and Barisani-Asenbauer, Talin", year = "2016", abstract = "The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Microbes and Infection", title = "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection", pages = "262-254", number = "4", volume = "18", doi = "10.1016/j.micinf.2015.12.001" }
Belij-Rammerstorfer, S., Inić-Kanada, A., Stojanović, M., Marinković, E., Lukić, I., Stein, E., Montanaro, J., Bintner, N., Schuerer, N., Ghasemian, E., Kundi, M.,& Barisani-Asenbauer, T.. (2016). Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection Elsevier Science Bv, Amsterdam., 18(4), 254-262. https://doi.org/10.1016/j.micinf.2015.12.001
Belij-Rammerstorfer S, Inić-Kanada A, Stojanović M, Marinković E, Lukić I, Stein E, Montanaro J, Bintner N, Schuerer N, Ghasemian E, Kundi M, Barisani-Asenbauer T. Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection. 2016;18(4):254-262. doi:10.1016/j.micinf.2015.12.001 .
Belij-Rammerstorfer, Sandra, Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Lukić, Ivana, Stein, Elisabeth, Montanaro, Jacqueline, Bintner, Nora, Schuerer, Nadine, Ghasemian, Ehsan, Kundi, Michael, Barisani-Asenbauer, Talin, "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection" in Microbes and Infection, 18, no. 4 (2016):254-262, https://doi.org/10.1016/j.micinf.2015.12.001 . .