Recent data concerning antiphospholipid syndrome (APS) induction have shown that beta(2)-glycoprotein I (beta(2)GPI) binds lipopolysaccharide (LPS), which results in conformational changes, exposition of a cryptic epitope and possible pathological anti-beta(2)GPI antibody production. In order to investigate the effects of LPS on the induction of APS-related pathology, we performed hyperimmunization of BALB/c and C57BL/6 mice with LPS, alone or in combination with tetanus toxoid (TTd), a protein structurally similar to beta(2)GPI. We report that, although high affinity pathological anti-beta(2)GPI antibodies were produced in all groups of animals, the reproductive pathology was recorded only in mice that received both LPS and TTd, implying on the important roles of both infections and molecular mimicry in APS pathogenesis. Moreover, APS-related reproductive pathology was more pronounced in BALB/c (lowered fertility and fecundity) than C57BL/6 mice (lowered fecundity), which correlated w...ell with the disruption in natural antibody network observed in BALB/c mouse strain.
TY - JOUR
AU - Petrušić, Vladimir
AU - Todorović, Nevena
AU - Živković, Irena
AU - Dimitrijević, Rajna
AU - Muhandes, Lina
AU - Rajnpreht, Irena
AU - Dimitrijević, Ljiljana
PY - 2015
UR - http://intor.torlakinstitut.com/handle/123456789/446
AB - Recent data concerning antiphospholipid syndrome (APS) induction have shown that beta(2)-glycoprotein I (beta(2)GPI) binds lipopolysaccharide (LPS), which results in conformational changes, exposition of a cryptic epitope and possible pathological anti-beta(2)GPI antibody production. In order to investigate the effects of LPS on the induction of APS-related pathology, we performed hyperimmunization of BALB/c and C57BL/6 mice with LPS, alone or in combination with tetanus toxoid (TTd), a protein structurally similar to beta(2)GPI. We report that, although high affinity pathological anti-beta(2)GPI antibodies were produced in all groups of animals, the reproductive pathology was recorded only in mice that received both LPS and TTd, implying on the important roles of both infections and molecular mimicry in APS pathogenesis. Moreover, APS-related reproductive pathology was more pronounced in BALB/c (lowered fertility and fecundity) than C57BL/6 mice (lowered fecundity), which correlated well with the disruption in natural antibody network observed in BALB/c mouse strain.
PB - Taylor & Francis Ltd, Abingdon
T2 - Autoimmunity
T1 - Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice
EP - 99
IS - 2
SP - 87
VL - 48
DO - 10.3109/08916934.2014.961061
ER -
@article{
author = "Petrušić, Vladimir and Todorović, Nevena and Živković, Irena and Dimitrijević, Rajna and Muhandes, Lina and Rajnpreht, Irena and Dimitrijević, Ljiljana",
year = "2015",
abstract = "Recent data concerning antiphospholipid syndrome (APS) induction have shown that beta(2)-glycoprotein I (beta(2)GPI) binds lipopolysaccharide (LPS), which results in conformational changes, exposition of a cryptic epitope and possible pathological anti-beta(2)GPI antibody production. In order to investigate the effects of LPS on the induction of APS-related pathology, we performed hyperimmunization of BALB/c and C57BL/6 mice with LPS, alone or in combination with tetanus toxoid (TTd), a protein structurally similar to beta(2)GPI. We report that, although high affinity pathological anti-beta(2)GPI antibodies were produced in all groups of animals, the reproductive pathology was recorded only in mice that received both LPS and TTd, implying on the important roles of both infections and molecular mimicry in APS pathogenesis. Moreover, APS-related reproductive pathology was more pronounced in BALB/c (lowered fertility and fecundity) than C57BL/6 mice (lowered fecundity), which correlated well with the disruption in natural antibody network observed in BALB/c mouse strain.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Autoimmunity",
title = "Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice",
pages = "99-87",
number = "2",
volume = "48",
doi = "10.3109/08916934.2014.961061"
}
Petrušić, V., Todorović, N., Živković, I., Dimitrijević, R., Muhandes, L., Rajnpreht, I.,& Dimitrijević, L.. (2015). Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice. in Autoimmunity
Taylor & Francis Ltd, Abingdon., 48(2), 87-99.
https://doi.org/10.3109/08916934.2014.961061
Petrušić V, Todorović N, Živković I, Dimitrijević R, Muhandes L, Rajnpreht I, Dimitrijević L. Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice. in Autoimmunity. 2015;48(2):87-99.
doi:10.3109/08916934.2014.961061 .
Petrušić, Vladimir, Todorović, Nevena, Živković, Irena, Dimitrijević, Rajna, Muhandes, Lina, Rajnpreht, Irena, Dimitrijević, Ljiljana, "Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice" in Autoimmunity, 48, no. 2 (2015):87-99,
https://doi.org/10.3109/08916934.2014.961061 . .
