Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid
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2013
Authors
Stojanović, Marijana
Petrušić, Vladimir
Živković, Irena
Inić-Kanada, Aleksandra

Stojičević, Ivana
Marinković, Emilija
Dimitrijević, Ljiljana
Article (Published version)

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Show full item recordAbstract
It is known that tetanus toxoid (TTd)-hyperimmunization induces increased titer of sera beta 2-glycoprotein I (beta 2GPI)-specific antibodies (Abs) in Balb/c mice. The concentrations of such induced anti-beta 2GPI Abs as well as their pathogenic potential are strongly influenced by the context of TTd application. beta 2GPI-specific immune response is established as a part of TTd-specific immune response by molecular mimicry mechanism due to structural homology between TTd and beta 2GPI. This finding is supported by the following facts: (1) cross-reactive Abs that recognize both TTd and beta 2GPI epitopes are present in Balb/c mice sera; (2) anti-TTd Abs secretion in splenic cultures is induced after beta 2GPI stimulation and vice versa. However, analyses of (1) IL-10 production following in vitro stimulation of immunized Balb/c mice splenocytes by TTd, beta 2GPI or glutaraldehyde-treated beta 2GPI and (2) specific impact of ConA and agonists of TLR2, TLR4, and TLR9 on anti-TTd and auto...reactive Abs secretion strongly imply that these two branches of the TTd-induced immune response do not use identical cell populations and are regulated in a different way. Results presented in this paper describe that structural homology between foreign and self-antigens could focus mounted autoreactive immune response toward specific self-structure, but the context of antigen application, including a history of previous immune stimulations and adjuvants applied together with the antigen, are the main factors which determine the outcome of the induced immune response.
Keywords:
Tetanus toxoid / beta 2-Glycoprotein I / Molecular mimicry / Polyclonal cell activationSource:
Immunologic Research, 2013, 56, 1, 20-31Publisher:
- Humana Press Inc, Totowa
Funding / projects:
DOI: 10.1007/s12026-012-8343-1
ISSN: 0257-277X
PubMed: 22875539