One of the established animal models for autoimmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein beta(2)GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-beta(2)GPI) antibodies induced in BALB/c mice during secondary immune response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoimmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-beta(2)GPI IgG antibodies, and (3) change in fecundity and fertility during secondary immune response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outc...ome, this time associated with the processes involving low affinity, natural antibodies.
TY - JOUR
AU - Živković, Irena
AU - Petrušić, Vladimir
AU - Dimitrijević, Rajna
AU - Stojanović, Marijana
AU - Dimitrijević, Ljiljana
PY - 2013
UR - http://intor.torlakinstitut.com/handle/123456789/394
AB - One of the established animal models for autoimmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein beta(2)GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-beta(2)GPI) antibodies induced in BALB/c mice during secondary immune response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoimmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-beta(2)GPI IgG antibodies, and (3) change in fecundity and fertility during secondary immune response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.
PB - Humana Press Inc, Totowa
T2 - Immunologic Research
T1 - Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice
EP - 149
IS - 1
SP - 143
VL - 56
DO - 10.1007/s12026-012-8378-3
ER -
@article{
author = "Živković, Irena and Petrušić, Vladimir and Dimitrijević, Rajna and Stojanović, Marijana and Dimitrijević, Ljiljana",
year = "2013",
abstract = "One of the established animal models for autoimmune disease antiphospholipid syndrome (APS) is TTd hyperimmunization of mice. Tetanus toxoid (TTd) and plasma protein beta(2)GPI share structural homology so that immunization with TTd induces appearance of cross-reactive antibodies. In this paper, we have investigated the presence and dynamic of fluctuation of specific (anti-TTd) and auto (anti-beta(2)GPI) antibodies induced in BALB/c mice during secondary immune response after TTd immunization with alhydrogel or glycerol as adjuvants. In addition, we followed the induced reproductive pathology as a sign of autoimmune outcome. We show undoubtedly adjuvant dependance of (1) level of induced anti-TTd IgG antibodies, (2) changes in levels of low-affinity anti-beta(2)GPI IgG antibodies, and (3) change in fecundity and fertility during secondary immune response. These findings once more indicate the importance of chosen adjuvants used for successful immunization and eventual autoantibody outcome, this time associated with the processes involving low affinity, natural antibodies.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice",
pages = "149-143",
number = "1",
volume = "56",
doi = "10.1007/s12026-012-8378-3"
}
Živković, I., Petrušić, V., Dimitrijević, R., Stojanović, M.,& Dimitrijević, L.. (2013). Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice. in Immunologic Research
Humana Press Inc, Totowa., 56(1), 143-149.
https://doi.org/10.1007/s12026-012-8378-3
Živković I, Petrušić V, Dimitrijević R, Stojanović M, Dimitrijević L. Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice. in Immunologic Research. 2013;56(1):143-149.
doi:10.1007/s12026-012-8378-3 .
Živković, Irena, Petrušić, Vladimir, Dimitrijević, Rajna, Stojanović, Marijana, Dimitrijević, Ljiljana, "Adjuvant dependence of APS pathology-related low-affinity antibodies during secondary immune response to tetanus toxoid in BALB/c mice" in Immunologic Research, 56, no. 1 (2013):143-149,
https://doi.org/10.1007/s12026-012-8378-3 . .
