Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic
Autori
Maslovarić, IrinaVukov, Nataša
Stojković, Aleksandra
Kosanović, Dejana
Jovanova-Nešić, Katica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mic...e.
Ključne reči:
EAE / MOG / lymph nodes / spleenIzvor:
Archives of Biological Sciences, 2013, 65, 4, 1317-1322Izdavač:
- Srpsko biološko društvo, Beograd, i dr.
Finansiranje / projekti:
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
- Plastičnost imunskog sistema tokom starenja: imunomodulatorni potencijal estrogena (RS-MESTD-Basic Research (BR or ON)-175050)
DOI: 10.2298/ABS1304317M
ISSN: 0354-4664
WoS: 000330326600007
Scopus: 2-s2.0-84884697733
Institucija/grupa
TorlakTY - JOUR AU - Maslovarić, Irina AU - Vukov, Nataša AU - Stojković, Aleksandra AU - Kosanović, Dejana AU - Jovanova-Nešić, Katica PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/368 AB - Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic EP - 1322 IS - 4 SP - 1317 VL - 65 DO - 10.2298/ABS1304317M ER -
@article{ author = "Maslovarić, Irina and Vukov, Nataša and Stojković, Aleksandra and Kosanović, Dejana and Jovanova-Nešić, Katica", year = "2013", abstract = "Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic", pages = "1322-1317", number = "4", volume = "65", doi = "10.2298/ABS1304317M" }
Maslovarić, I., Vukov, N., Stojković, A., Kosanović, D.,& Jovanova-Nešić, K.. (2013). Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(4), 1317-1322. https://doi.org/10.2298/ABS1304317M
Maslovarić I, Vukov N, Stojković A, Kosanović D, Jovanova-Nešić K. Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences. 2013;65(4):1317-1322. doi:10.2298/ABS1304317M .
Maslovarić, Irina, Vukov, Nataša, Stojković, Aleksandra, Kosanović, Dejana, Jovanova-Nešić, Katica, "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic" in Archives of Biological Sciences, 65, no. 4 (2013):1317-1322, https://doi.org/10.2298/ABS1304317M . .