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Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62

Authorized Users Only
2012
Authors
Petrušić, Vladimir
Živković, Irena
Stojanović, Marijana
Lukić, Ivana
Marinković, Emilija
Dimitrijević, Ljiljana
Article (Published version)
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Abstract
Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as r...etrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments. (C) 2011 Elsevier GmbH. All rights reserved.

Keywords:
Hybridoma technology / Immunohistochemistry / Monoclonal antibody / PC 12 cells / Tetanus toxin
Source:
Acta Histochemica, 2012, 114, 5, 480-486
Publisher:
  • Elsevier Gmbh, Urban & Fischer Verlag, Jena
Funding / projects:
  • Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)

DOI: 10.1016/j.acthis.2011.09.001

ISSN: 0065-1281

PubMed: 21975055

WoS: 000305303900009

Scopus: 2-s2.0-84860386322
[ Google Scholar ]
5
4
URI
http://intor.torlakinstitut.com/handle/123456789/349
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Stojanović, Marijana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Dimitrijević, Ljiljana
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/349
AB  - Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as retrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments. (C) 2011 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Acta Histochemica
T1  - Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62
EP  - 486
IS  - 5
SP  - 480
VL  - 114
DO  - 10.1016/j.acthis.2011.09.001
ER  - 
@article{
author = "Petrušić, Vladimir and Živković, Irena and Stojanović, Marijana and Lukić, Ivana and Marinković, Emilija and Dimitrijević, Ljiljana",
year = "2012",
abstract = "Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as retrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments. (C) 2011 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Acta Histochemica",
title = "Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62",
pages = "486-480",
number = "5",
volume = "114",
doi = "10.1016/j.acthis.2011.09.001"
}
Petrušić, V., Živković, I., Stojanović, M., Lukić, I., Marinković, E.,& Dimitrijević, L.. (2012). Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62. in Acta Histochemica
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 114(5), 480-486.
https://doi.org/10.1016/j.acthis.2011.09.001
Petrušić V, Živković I, Stojanović M, Lukić I, Marinković E, Dimitrijević L. Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62. in Acta Histochemica. 2012;114(5):480-486.
doi:10.1016/j.acthis.2011.09.001 .
Petrušić, Vladimir, Živković, Irena, Stojanović, Marijana, Lukić, Ivana, Marinković, Emilija, Dimitrijević, Ljiljana, "Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62" in Acta Histochemica, 114, no. 5 (2012):480-486,
https://doi.org/10.1016/j.acthis.2011.09.001 . .

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