Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes
Само за регистроване кориснике
2011
Аутори
Stenovec, MatjazMilošević, Milena

Petrušić, Vladimir
Potokar, Maja
Stević, Zorica
Prebil, Mateja
Kreft, Marko
Trkov, Saša
Anđus, Pavle
Zorec, Robert

Чланак у часопису (Објављена верзија)

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Aim: We examined the effect of purified immunoglobulins G (IgG) from patients with amyotrophic lateral sclerosis (ALS) on the mobility and exocytotic release from Lysotracker-stained vesicles in cultured rat astrocytes. Methods: Time-lapse confocal images were acquired, and vesicle mobility was analysed before and after the application of ALS IgG. The vesicle counts were obtained to assess cargo exocytosis from stained organelles. Results: At rest, when mobility was monitored for 2 min in bath with Ca2+, two vesicle populations were discovered: (1) non-mobile vesicles (6.1%) with total track length (TL) lt 1 mu m, averaging at 0.33 +/- 0.01 mu m (n = 1305) and (2) mobile vesicles (93.9%) with TL gt 1 mu m, averaging at 3.03 +/- 0.01 mu m (n = 20 200). ALS IgG (0.1 mg mL(-1)) from 12 of 13 patients increased the TL of mobile vesicles by approx. 24% and maximal displacement (MD) by approx. 26% within 4 min, while the IgG from control group did not alter the vesicle mobility. The mobi...lity enhancement by ALS IgG was reduced in extracellular solution devoid of Ca2+, indicating that ALS IgG vesicle mobility enhancement involves changes in Ca2+ homeostasis. To examine whether enhanced mobility relates to elevated Ca2+ activity, cells were stimulated by 1 mm ATP, a cytosolic Ca2+ increasing agent, in the presence (2 mm) and in the absence of extracellular Ca2+. ATP stimulation triggered an increase in TL by approx. 7% and 12% and a decrease in MD by approx. 11% and 1%, within 4 min respectively. Interestingly, none of the stimuli triggered the release of vesicle cargo. Conclusion: Amyotrophic lateral sclerosis-IgG-enhanced vesicle mobility in astrocytes engages changes in calcium homeostasis.
Кључне речи:
amyotrophic lateral sclerosis / astrocytes / endosomes / IgG / lysosomes / vesicle mobilityИзвор:
Acta Physiologica, 2011, 203, 4, 457-471Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Slovenian Research Agency (ARRS)Slovenian Research Agency - Slovenia [P3 310 0381]
- EduGlia ESR
- NEUROIMAGE SSA-INCO [024600, 41005]
DOI: 10.1111/j.1748-1716.2011.02337.x
ISSN: 1748-1708
PubMed: 21726417